A statistical approach for detecting genomic aberrations in heterogeneous tumor samples from single nucleotide polymorphism genotyping data

We describe a statistical method for the characterization of genomic aberrations in single nucleotide polymorphism microarray data acquired from cancer genomes. Our approach allows us to model the joint effect of polyploidy, normal DNA contamination and intra-tumour heterogeneity within a single unified Bayesian framework. We demonstrate the efficacy of our method on numerous datasets including laboratory generated mixtures of normal-cancer cell lines and real primary tumours

Review and discussion: E-learning for academia and industry

This paper presents a high level review and discussion about e-learning and proposes the use of interactive learning as a recommended method for staff training in industry and academia. Interactive learning is focused on the integrated e-learning and face-to-face learning to ensure that the process of learning can stimulate learners’ interests, report their progress and have tutors to provide their feedback and guide learners to the expected targets. Learning activities and varieties have been illustrated with discussion about how industry and academia can use interactive learning. Five successful examples of interactive learning to demonstrate the effectiveness of interactive learning. Positive impacts have been reported in RBS, SMEs using SAP, University of Cambridge, University of Greenwich and Leeds Beckett University to support the positive outcomes for learners and trainers. Future directions have been discussed, particularly the use of emerging services can enhance the learning experience and satisfaction for learners and trainers

Glucose utilization via glycogen phosphorylase sustains proliferation and prevents premature senescence in cancer cells.

Metabolic reprogramming of cancer cells provides energy and multiple intermediates critical for cell growth. Hypoxia in tumors represents a hostile environment that can encourage these transformations. We report that glycogen metabolism is upregulated in tumors in vivo and in cancer cells in vitro in response to hypoxia. In vitro, hypoxia induced an early accumulation of glycogen, followed by a gradual decline. Concordantly, glycogen synthase (GYS1) showed a rapid induction, followed by a later increase of glycogen phosphorylase (PYGL). PYGL depletion and the consequent glycogen accumulation led to increased reactive oxygen species (ROS) levels that contributed to a p53-dependent induction of senescence and markedly impaired tumorigenesis in vivo. Metabolic analyses indicated that glycogen degradation by PYGL is important for the optimal function of the pentose phosphate pathway. Thus, glycogen metabolism is a key pathway induced by hypoxia, necessary for optimal glucose utilization, which represents a targetable mechanism of metabolic adaptation

Evaluating university industry collaborative research centers

This research provides performance metrics for cooperative research centers that enhance translational research through partnerships formed by government, industry and academia. Centers are part of complex ecosystems and vary greatly in the type of science conducted, organizational structures and expected outcomes. The ability to realize their objectives depends on transparent measurement systems to assist in decision making in research translation. We introduce a hierarchical decision model that uses both quantitative and qualitative metrics. A generalizable model is developed based upon program goals. The results are validated through consultation with experts. The method is illustrated using data from the National Science Foundation's industry/university cooperative research center (IUCRC) program. The methodology provides a basis for a generalizable model and measurement system to compares performance of university science and engineering focused research centers supported by industry and government

Masturbación femenina y masculina en adulto joven: beneficios y tabúes

Curso de Especial Interés: Psicología y SexualidadEste trabajo muestra una revisión teórica sobre la importancia de la masturbación en la vida del ser humano, abordando algunas de las posturas que existen y teniendo en cuenta que hay muchos prejuicios y tabúes al momento de mencionar este tema. Se encontró que la mayoría de los participantes tienen un conocimiento previo sobre el tema.RESUMEN JUSTIFICACIÓN 1. DESARROLLO DE LA INFORMACIÓN 2. METODOLOGÍA 3. ESTUDIO DE MERCADEO 4. RESULTADOS 5. CONCLUSIONES REFERENCIASPregradoPsicólog

No evidence of association between apolipoprotein E genotype and phenotypic severity in childhood onset proximal spinal muscular atrophy

The survival motor neuron (SMN) gene is present in two copies on chromosome 5q13 and the evidence is now compelling that mutations in the telomeric copy (SMNt) of the gene underlie childhood onset proximal spinal muscular atrophy (SMA). There is a correlation between the number of centromeric SMN gene copies (SMNc) and the clinical severity of the disease but this relationship is not absolute. Allelic variants of the apolipoprotein E (APOE) gene encoded on chromosome 19q are known to influence the prognosis and risk in a number of neurological disorders. We have therefore genotyped 166 unrelated cases of SMA to determine whether the presence of specific APOE genotypes correlates with severity of disease. The study failed to show the influence of any particular APOE genotype on disease severity, with specifically APOE ?4 being no more common in the milder SMA forms and APOE ?2 not over represented in type I SMA. A limited study of 23 SMA families also failed to show any influence of APOE genotype on SMA disease severity. Factors other than APOE genotype must therefore be responsible for determining SMA disease severity