New bodipy dyads for two-photons fluorescence imaging in cells

Fluorescent probes are essential tools for studying biological systems. The last decade has witnessed particular interest in the development of two-photon excitable probes, due to their advantageous features in tissue imaging compared to the corresponding one-photon probes [1]. Recently, we have designed and synthetized an aminonaphthalimide–BODIPY derivative as energy transfer cassettes and were found to show very fast and efficient BODIPY fluorescence sensitization [2]. This was observed upon one- and two-photon excitation, which extends the application range of the investigated bichromophoric dyads in terms of accessible excitation wavelengths. In order to increase the two-photon absorption of the system aminonaphthalimide fluorophore was replace with a Prodan analog (BODIPY dyad 1), which presents found a variety of applications as probes and labels in biology [3]. The two-photon absorption cross-section of the dyads is significantly incremented by the presence of the 6-acetyl-2-naphthylamine donor group. The emission maximum of a BODIPY fluorophore can significantly be red-shifted in comparison to their precursors by conjugation with aromatic aldehydes. [4] We use a synthetic strategy to obtain BODIPY dyad 2 that incorporates an imidazole ring. This molecule can be used in biological media as a near-neutral pH indicator based on one- and two-photon excitable BODIPY acceptor.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Two-Photon Fluorescence As Tool For Imaging In Cells

Fluorescent probes are essential tools for studying biological systems. The last decade has witnessed particular interest in the development of two-photon excitable probes, due to their advantageous features in tissue imaging compared to one-photon probes [1]. Recently, we have designed and synthesized an aminonaphthalimide–BODIPY derivative as energy transfer cassette which was found to show very fast and efficient BODIPY fluorescence sensitization [2]. This was observed upon one- and two-photon excitation, which extends the application range of the investigated bichromophoric dyads in terms of accessible excitation wavelengths. In order to increase the two-photon absorption of the system the aminonaphthalimide fluorophore was replaced with a Prodan analogue, which presents a variety of applications as probes and labels in biology [3]. The two-photon absorption cross-section of the dyads is significantly incremented by the presence of the 6-acetyl-2-naphthylamine donor group. We also explore in this communication the use of new fluorophores based on four-coordinate organoboron N,C-chelates containing an arylisoquinoline skeleton in confocal fluorescence microscopy that show significant two-photon absorption cross sections and allow the use of excitation wavelengths in the near-infrared region [4].References [1] H. M. Kim, B. R. Cho, Chem. Rev. 2015, 5014–5055. [2] D. Collado, P. Remón, Y. Vida, F. Nájera, P. Sen, U. Pischel, E. Perez-Inestrosa, Chem. Asian J. 2014, 9, 797–804. [3] O. A. Kucherak, P. Didier, Y. Mely, A. S. Klymchenko, J. Phys. Chem. Lett. 2010, 1, 616–620. [4] V. F. Pais, M. M. Alcaide, R. López-Rodriguez, D. Collado, F. Nájera, E. Perez-Inestrosa, E. Álvarez, J. M. Lassaletta, R. Fernández, A. Ros, U. Pischel, Chem. Eur. J. 2015, 21, 15369–15376.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Separability and aggregate shocks in the life-cycle model of consumption: evidence from Spain

The purpose of this paper is to test the life-cycle permanent income hypothesis using an unbalanced panel from the Spanish family expenditure survey. Our model accounts for aggregate shocks and non-separability in the Euler equation among consumption goods, contrary to most of the Literature in this area. Our results do not indicate excess sensitivity of consumption growth to income

INNOVATION AND JOB CREATION AND DESTRUCTION: EVIDENCE FROM SPAIN

In this paper we examine the effect of innovation on job creation and job destruction in Spanish manufacturing. Our empirical analysis is based on firm-level longitudinal data from which we have information on employment and innovation activity. The estimation approach consists of a two-step procedure that takes into account the fact that firms endogenously choose positive, negative or zero growth in employment, in which the selection mechanism is an ordered probit. Our results point out the importance of innovation variables on employment growth: innovative firms create more jobs -and destroy fewer- than non-innovative, and the degree of technological effort has a strong positive effect on net employment creation.Labour Demand; Technological Innovation; Sample Selection.

Goals/questions/metrics method and SAP implementation projects

During the last years some researchers have studied the critical success factors (CSFs) in ERP implementations. However, until now, no one has studied how these CSFs should be put in practice to help organizations achieve success in ERP implementations. This technical research report attempts to define the usage of Goals/Questions/Metrics (GQM) approach in the definition of a measurement system for ERP implementation projects. GQM approach is a mechanism for defining and interpreting operational, measurable goals. Lately, because of its intuitive nature the approach has gained widespread appeal. We present a metrics overview and a description of GQM approach. Then we provide an example of GQM application for monitoring sustained management support in ERP implementations. Sustained management support is the most cited critical success factor in ERP implementation projects.Postprint (published version

Innovation and job creation and destruction : evidence from Spain

In this paper we examine the effect of innovation on job creation and job destruction in Spanish manufacturing. Our empirical analysis is based on firm-level longitudinal data from which we have information on employment and innovation activity. The estimation approach consists of a two-step procedure that takes into account the fact that firms endogenously choose positive, negative or zero growth in employment, in which the selection mechanism is an ordered probit. Our results point out the importance of innovation variables on employment growth: innovative firms create more jobs -and destroy fewer- than non-innovative, and the degree of technological effort has a strong positive effect on net employment creation

Getting Back on Track: Effects of a Community College Program for Probationary Students

Rates of graduation and degree completion at community colleges remain distressingly low. This report evaluates two versions of a program designed to help probationary students at community college succeed in school. One version increased the average number of credits earned, the proportion of students who earned a grade point average of "C" or higher, and the proportion who moved off probation

-THE RESPONSE OF EXPENDITURES TO ANTICIPATED INCOME CHANGES: PANEL DATA ESTIMATES

Standard models of intertemporal allocation predict that the time path of expenditures should beindependent of the time path of income. Recently two papers, Parker (1999) and Souleles (1999)have suggested that U.S. households have a high marginal propensity to spend within yearanticipated income changes. We use an expenditure survey panel from Spain to re-examine thisissue. We exploit two important features of the Spanish data. First, we have quarterly panel datathat follows households for more than four quarters. Second, we use the fact that workers areexogenously sorted into one of two payment schemes: some receive the same amount eachmonth of the year and others receive an extra payment in June and December. The extra paymentis large and predictable. We examine the detailed pattern of expenditures over the year to seewhether they differ between the two groups. We fail to find even weak differences. Wecomplement this with a conventional Euler equation analysis of excess sensitivity. Our predictingequation for (quarterly) earnings growth is much better than usual and is likely to give a powerfultest of the hypothesis that predictable changes in income do not lead to changes in expenditurepatterns. The results of this analysis confirm the graphical analysis: we find no evidence ofexcess sensitivity. We conclude that households in normal times do smooth consumption overthe year. We suggest a reconciliation of our results with those of Parker and Souleles.Consumption; Excess Sensitivity; Smoothing.