“Don’t Shoot The Unicorn!” Finding Transformative Happiness and Resilience Through Developing Your “Anditude”!

As a care provider and leader in your community, you understand the challenge of keeping your own resilience high, and maintaining an attitude of hope. This transformational, uplifting program will equip you to develop the skill of happiness, empowering you to lead youths by demonstrating positive approaches in your own life, and impacting them with “Infectious Resilience” as they learn to adopt your Anditude

You Live, You Lose: Supporting Youths on Their Journeys in the Land of the Loss

As youths journey through life, they experience various personally significant losses and associated grief that can negatively impact their physical/mental health, developmental trajectory, and academic success. Framed in a broad-based perspective of loss, this presentation will acquaint participants with various loss-related constructs and events, potential associated physical, intellectual, emotional, and social effects of loss experiences, and supportive interventions and resources

Next Generation Mapping of Enological Traits in an F2 Interspecific Grapevine Hybrid Family

In winegrapes (Vitis spp.), fruit quality traits such as berry color, total soluble solids content (SS), malic acid content (MA), and yeast assimilable nitrogen (YAN) affect fermentation or wine quality, and are important traits in selecting new hybrid winegrape cultivars. Given the high genetic diversity and heterozygosity of Vitis species and their tendency to exhibit inbreeding depression, linkage map construction and quantitative trait locus (QTL) mapping has relied on F1 families with the use of simple sequence repeat (SSR) and other markers. This study presents the construction of a genetic map by single nucleotide polymorphisms identified through genotyping-by-sequencing (GBS) technology in an F2 mapping family of 424 progeny derived from a cross between the wild species V. riparia Michx. and the interspecific hybrid winegrape cultivar, ‘Seyval’. The resulting map has 1449 markers spanning 2424 cM in genetic length across 19 linkage groups, covering 95% of the genome with an average distance between markers of 1.67 cM. Compared to an SSR map previously developed for this F2 family, these results represent an improved map covering a greater portion of the genome with higher marker density. The accuracy of the map was validated using the well-studied trait berry color. QTL affecting YAN, MA and SS related traits were detected. A joint MA and SS QTL spans a region with candidate genes involved in the malate metabolism pathway. We present an analytical pipeline for calling intercross GBS markers and a high-density linkage map for a large F2 family of the highly heterozygous Vitis genus. This study serves as a model for further genetic investigations of the molecular basis of additional unique characters of North American hybrid wine cultivars and to enhance the breeding process by marker-assisted selection. The GBS protocols for identifying intercross markers developed in this study can be adapted for other heterozygous species

“Don’t Shoot The Unicorn!” How to find true happiness and resilience along your self-care journey.

“As a care-provider and leader in your community, you understand the challenge of keeping your own resilience high, and maintaining an attitude of hope. This transformational, uplifting program will equip you to develop the skill of happiness, empowering you to lead youth by demonstrating positive approaches in your own life, and impacting them with “Infectious Resilience” as they learn to adopt your “Anditude!

Being a Lighthouse on the Shore of the Stormy Sea of Grief: Supporting Loss-Affected Youth

As children and adolescents journey through life, they experience personally significant losses and associated grief that can negatively impact their physical/mental health, optimal development, and learning. Applying a broad-based perspective of loss, this presentation will acquaint educators/youth workers with (a) loss-related constructs and events, (b) potential physical, intellectual, emotional, and social effects of loss experiences, (c) supportive interventions, and (d) recommended print and Internet-based resources

Wireless Instantaneous Neurotransmitter Concentration Sensing System (WINCS) for intraoperative neurochemical monitoring

The Wireless Instantaneous Neurotransmitter Concentration Sensing System (WINCS) measures extracellular neurotransmitter concentration in vivo and displays the data graphically in nearly real time. WINCS implements two electroanalytical methods, fast-scan cyclic voltammetry (FSCV) and fixed-potential amperometry (FPA), to measure neurotransmitter concentrations at an electrochemical sensor, typically a carbon-fiber microelectrode. WINCS comprises a battery-powered patient module and a custom software application (WINCSware) running on a nearby personal computer. The patient module impresses upon the electrochemical sensor either a constant potential (for FPA) or a time-varying waveform (for FSCV). A transimpedance amplifier converts the resulting current to a signal that is digitized and transmitted to the base station via a Bluetooth® radio link. WINCSware controls the operational parameters for FPA or FSCV, and records the transmitted data stream. Filtered data is displayed in various formats, including a background-subtracted plot of sequential FSCV scans - a representation that enables users to distinguish the signatures of various analytes with considerable specificity. Dopamine, glutamate, adenosine and serotonin were selected as analytes for test trials. Proof-of-principle tests included in vitro flow-injection measurements and in vivo measurements in rat and pig. Further testing demonstrated basic functionality in a 3-Tesla MRI unit. WINCS was designed in compliance with consensus standards for medical electrical device safety, and it is anticipated that its capability for real-time intraoperative monitoring of neurotransmitter release at an implanted sensor will prove useful for advancing functional neurosurgery