90 research outputs found
T:G mismatch-specific thymine-DNA glycosylase (TDG) as a coregulator of transcription interacts with SRC1 family members through a novel tyrosine repeat motif
Gene activation involves protein complexes with diverse enzymatic activities, some of which are involved in chromatin modification. We have shown previously that the base excision repair enzyme thymine DNA glycosylase (TDG) acts as a potent coactivator for estrogen receptor-α. To further understand how TDG acts in this context, we studied its interaction with known coactivators of nuclear receptors. We find that TDG interacts in vitro and in vivo with the p160 coactivator SRC1, with the interaction being mediated by a previously undescribed motif encoding four equally spaced tyrosine residues in TDG, each tyrosine being separated by three amino acids. This is found to interact with two motifs in SRC1 also containing tyrosine residues separated by three amino acids. Site-directed mutagenesis shows that the tyrosines encoded in these motifs are critical for the interaction. The related p160 protein TIF2 does not interact with TDG and has the altered sequence, F-X-X-X-Y, at the equivalent positions relative to SRC1. Substitution of the phenylalanines to tyrosines is sufficient to bring about interaction of TIF2 with TDG. These findings highlight a new protein-protein interaction motif based on Y-X-X-X-Y and provide new insight into the interaction of diverse proteins in coactivator complexe
A portal of educational resources: providing evidence for matching pedagogy with technology
The TPACK (Technology, Pedagogy and Content Knowledge) model presents the three types of knowledge that are necessary to implement a successful technology-based educational activity. It highlights how the intersections between TPK (Technological Pedagogical Knowledge), PCK (Pedagogical Content Knowledge) and TCK (Technological Content Knowledge) are not a sheer sum up of their components but new types of knowledge. This paper focuses on TPK, the intersection between technology knowledge and pedagogy knowledge â a crucial field of investigation. Actually, technology in education is not just an add-on but is literally reshaping teaching/learning paradigms. Technology modifies pedagogy and pedagogy dictates requirements to technology. In order to pursue this research, an empirical approach was taken, building a repository (back-end) and a portal (front-end) of about 300 real-life educational experiences run at school. Educational portals are not new, but they generally emphasise content. Instead, in our portal, technology and pedagogy take centre stage. Experiences are classified according to more than 30 categories (âfacetsâ) and more than 200 facet values, all revolving around the pedagogical implementation and the technology used. The portal (an innovative piece of technology) supports sophisticated âexploratoryâ sessions of use, targeted at researchers (investigating the TPK intersection), teachers (looking for inspiration in their daily jobs) and decision makers (making decisions about the introduction of technology into schools)
A measurement of the muon number in showers using inclined events recorded at the Pierre Auger Observatory
The average muon content of air showers with zenith angles exceeding 62⊠is obtained as a function of calorimetric energy from events measured simultaneously with the Surface Detector Array and fluorescence telescopes of the Pierre Auger Observatory using a reconstruction method specifically designed for inclined showers. The results are presented in different energy bins above 4E18 eV and compared to predictions from current hadronic interaction models for different primary particles.Fil: Allekotte, Ingomar. Centro AtĂłmico Bariloche and Instituto Balseiro; ArgentinaFil: Almela, Daniel Alejandro. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Parque Centenario. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. ComisiĂłn Nacional de EnergĂa AtĂłmica. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. Universidad Nacional de San MartĂn. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas; ArgentinaFil: Bertou, Xavier Pierre Louis. Centro AtĂłmico Bariloche and Instituto Balseiro; ArgentinaFil: Dova, Maria Teresa. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Instituto de FĂsica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de FĂsica La Plata; ArgentinaFil: Figueira, Juan Manuel. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Parque Centenario. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. ComisiĂłn Nacional de EnergĂa AtĂłmica. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. Universidad Nacional de San MartĂn. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas; ArgentinaFil: Filevich, A.. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Parque Centenario. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. ComisiĂłn Nacional de EnergĂa AtĂłmica. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. Universidad Nacional de San MartĂn. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas; ArgentinaFil: Garcia, Beatriz Elena. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Parque Centenario. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. ComisiĂłn Nacional de EnergĂa AtĂłmica. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. Universidad Nacional de San MartĂn. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas; ArgentinaFil: Gomez Berisso, Mariano. Centro AtĂłmico Bariloche and Instituto Balseiro; ArgentinaFil: GĂłmez Vitale, Primo F.. Observatorio Pierre Auger; ArgentinaFil: GonzĂĄlez, N.. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Parque Centenario. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. ComisiĂłn Nacional de EnergĂa AtĂłmica. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. Universidad Nacional de San MartĂn. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas; ArgentinaFil: Hansen, P.. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Instituto de FĂsica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de FĂsica La Plata; ArgentinaFil: Tueros, Matias Jorge. Universidad de Santiago de Compostela. Facultad de FĂsica; España. Provincia de Buenos Aires. GobernaciĂłn. ComisiĂłn de Investigaciones CientĂficas. Instituto Argentino de RadioastronomĂa. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Instituto Argentino de RadioastronomĂa; ArgentinaFil: Harari, Diego Dario. Centro AtĂłmico Bariloche and Instituto Balseiro; ArgentinaFil: Jarne, C.. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Instituto de FĂsica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de FĂsica La Plata; ArgentinaFil: Mariazzi, Analisa Gabriela. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Instituto de FĂsica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de FĂsica La Plata; ArgentinaFil: Melo, Diego Gabriel. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Parque Centenario. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. ComisiĂłn Nacional de EnergĂa AtĂłmica. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. Universidad Nacional de San MartĂn. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas; ArgentinaFil: Micheletti, M. I.. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Rosario. Instituto de FĂsica de Rosario. Universidad Nacional de Rosario. Instituto de FĂsica de Rosario; ArgentinaFil: Mollerach, S.. Centro AtĂłmico Bariloche and Instituto Balseiro; ArgentinaFil: Pallotta, J.. Ministerio de Defensa. Instituto de Investigaciones CientĂficas y TĂ©cnicas para la Defensa; ArgentinaFil: Platino, Manuel. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Parque Centenario. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. ComisiĂłn Nacional de EnergĂa AtĂłmica. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. Universidad Nacional de San MartĂn. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas; ArgentinaFil: Quel, E. J.. Ministerio de Defensa. Instituto de Investigaciones CientĂficas y TĂ©cnicas para la Defensa; ArgentinaFil: Ristori, P.. Ministerio de Defensa. Instituto de Investigaciones CientĂficas y TĂ©cnicas para la Defensa; ArgentinaFil: Roulet, Esteban. Centro AtĂłmico Bariloche and Instituto Balseiro; ArgentinaFil: Rovero, Adrian Carlos. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Ciudad Universitaria. Instituto de AstronomĂa y FĂsica del Espacio(i); ArgentinaFil: SĂĄnchez, F.. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Parque Centenario. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. ComisiĂłn Nacional de EnergĂa AtĂłmica. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. Universidad Nacional de San MartĂn. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas; ArgentinaFil: Sato, R.. Observatorio Pierre Auger; ArgentinaFil: Sciutto, S. J.. Observatorio Pierre Auger; ArgentinaFil: Supanitsky, Alberto Daniel. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Ciudad Universitaria. Instituto de AstronomĂa y FĂsica del Espacio(i); ArgentinaFil: Tapia, A.. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Parque Centenario. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. ComisiĂłn Nacional de EnergĂa AtĂłmica. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. Universidad Nacional de San MartĂn. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas; ArgentinaFil: Videla, M.. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Parque Centenario. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. ComisiĂłn Nacional de EnergĂa AtĂłmica. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. Universidad Nacional de San MartĂn. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas; ArgentinaFil: Wahlberg, Hernan Pablo. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Instituto de FĂsica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de FĂsica La Plata; ArgentinaFil: Wainberg, Oscar Isaac. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Parque Centenario. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. ComisiĂłn Nacional de EnergĂa AtĂłmica. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas. Universidad Nacional de San MartĂn. Instituto de TecnologĂa en DetecciĂłn y AstropartĂculas; ArgentinaFil: Valiño, InĂ©s. Observatorio Pierre Auger; ArgentinaFil: The Pierre Auger Collaboration. No especifĂca;33rd International Cosmic Ray Conference (ICRC 2013)Rio de JaneiroBrasilInternational Union of Pure and Applied Physics (IUPAP
Early Detection, Diagnosis and Intervention Services for Young Children with Autism Spectrum Disorder in the European Union (ASDEU): Family and Professional Perspectives
Early services for ASD need to canvas the opinions of both parents and professionals. These opinions are seldom compared in the same research study. This study aims to ascertain the views of families and professionals on early detection, diagnosis and intervention services for young children with ASD. An online survey compiled and analysed data from 2032 respondents across 14 European countries (60.9% were parents; 39.1% professionals). Using an ordinal scale from 1 to 7, parentsâ opinions were more negative (mean = 4.6; SD 2.2) compared to those of professionals (mean = 4.9; SD 1.5) when reporting satisfaction with services. The results suggest services should take into account childâs age, delays in accessing services, and active stakeholdersâ participation when looking to improve services
Measurement of the cosmic ray spectrum above eV using inclined events detected with the Pierre Auger Observatory
A measurement of the cosmic-ray spectrum for energies exceeding
eV is presented, which is based on the analysis of showers
with zenith angles greater than detected with the Pierre Auger
Observatory between 1 January 2004 and 31 December 2013. The measured spectrum
confirms a flux suppression at the highest energies. Above
eV, the "ankle", the flux can be described by a power law with
index followed by
a smooth suppression region. For the energy () at which the
spectral flux has fallen to one-half of its extrapolated value in the absence
of suppression, we find
eV.Comment: Replaced with published version. Added journal reference and DO
Analysis of conservation priorities of Iberoamerican cattle based on autosomal microsatellite markers
Articles in International JournalsBackground: Determining the value of livestock breeds is essential to define conservation priorities, manage genetic diversity and allocate funds. Within- and between-breed genetic diversity need to be assessed to preserve the highest intra-specific variability. Information on genetic diversity and risk status is still lacking for many Creole cattle breeds from the Americas, despite their distinct evolutionary trajectories and adaptation to extreme environmental conditions.
Methods: A comprehensive genetic analysis of 67 Iberoamerican cattle breeds was carried out with 19 FAOrecommended
microsatellites to assess conservation priorities. Contributions to global diversity were investigated using alternative methods, with different weights given to the within- and between-breed components of genetic diversity. Information on Iberoamerican plus 15 worldwide cattle breeds was used to investigate the contribution of geographical breed groups to global genetic diversity.
Results: Overall, Creole cattle breeds showed a high level of genetic diversity with the highest level found in
breeds admixed with zebu cattle, which were clearly differentiated from all other breeds. Within-breed kinships
revealed seven highly inbred Creole breeds for which measures are needed to avoid further genetic erosion.
However, if contribution to heterozygosity was the only criterion considered, some of these breeds had the lowest priority for conservation decisions. The Weitzman approach prioritized highly differentiated breeds, such as GuabalĂĄ, Romosinuano, Cr. Patagonico, Siboney and CaracĂș, while kinship-based methods prioritized mainly zebu-related breeds. With the combined approaches, breed ranking depended on the weights given to the within- and
between-breed components of diversity. Overall, the Creole groups of breeds were generally assigned a higher
priority for conservation than the European groups of breeds.
Conclusions: Conservation priorities differed significantly according to the weight given to within- and betweenbreed
genetic diversity. Thus, when establishing conservation programs, it is necessary to also take into account other features. Creole cattle and local isolated breeds retain a high level of genetic diversity. The development of sustainable breeding and crossbreeding programs for Creole breeds, and the added value resulting from their
products should be taken into consideration to ensure their long-term survival
Global maps of soil temperature
Research in global change ecology relies heavily on global climatic grids derived from estimates of air temperature in open areas at around 2 m above the ground. These climatic grids do not reflect conditions below vegetation canopies and near the ground surface, where critical ecosystem functions occur and most terrestrial species reside. Here, we provide global maps of soil temperature and bioclimatic variables at a 1-kmÂČ resolution for 0â5 and 5â15 cm soil depth. These maps were created by calculating the difference (i.e., offset) between in-situ soil temperature measurements, based on time series from over 1200 1-kmÂČ pixels (summarized from 8500 unique temperature sensors) across all the worldâs major terrestrial biomes, and coarse-grained air temperature estimates from ERA5-Land (an atmospheric reanalysis by the European Centre for Medium-Range Weather Forecasts). We show that mean annual soil temperature differs markedly from the corresponding gridded air temperature, by up to 10°C (mean = 3.0 ± 2.1°C), with substantial variation across biomes and seasons. Over the year, soils in cold and/or dry biomes are substantially warmer (+3.6 ± 2.3°C) than gridded air temperature, whereas soils in warm and humid environments are on average slightly cooler (-0.7 ± 2.3°C). The observed substantial and biome-specific offsets emphasize that the projected impacts of climate and climate change on near-surface biodiversity and ecosystem functioning are inaccurately assessed when air rather than soil temperature is used, especially in cold environments. The global soil-related bioclimatic variables provided here are an important step forward for any application in ecology and related disciplines. Nevertheless, we highlight the need to fill remaining geographic gaps by collecting more in-situ measurements of microclimate conditions to further enhance the spatiotemporal resolution of global soil temperature products for ecological applications
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