SPR imaging biosensor for the 20S proteasome: sensor development and application to measurement of proteasomes in human blood plasma

The 20S proteasome is a multicatalytic enzyme complex responsible for intracellular protein degradation in mammalian cells. Its antigen level or enzymatic activity in blood plasma are potentially useful markers for various malignant and nonmalignant diseases. We have developed a method for highly selective determination of the 20S proteasome using a Surface Plasmon Resonance Imaging (SPRI) technique. It is based on the highly selective interaction between the proteasome’s catalytic β5 subunit and immobilized inhibitors (the synthetic peptide PSI and epoxomicin). Inhibitor concentration and pH were optimized. Analytical responses, linear ranges, accuracy, precision and interferences were investigated. Biosensors based on either PSI and epoxomicin were found to be suitable for quantitative determination of the proteasome, with a precision of ±10% for each, and recoveries of 102% and 113%, respectively, and with little interference by albumin, trypsin, chymotrypsin, cathepsin B and papain. The proteasome also was determined in plasma of healthy subjects and of patients suffering from acute leukemia. Both biosensors gave comparable results (2860 ng·mL-1 on average for control, and 42300 ng·mL-1 on average for leukemia patients)

Planck early results. VI. The High Frequency Instrument data processing

We describe the processing of the 336 billion raw data samples from the High Frequency Instrument (HFI) which we performed to produce six temperature maps from the first 295 days of Planck-HFI survey data. These maps provide an accurate rendition of the sky emission at 100, 143, 217, 353, 545 and 857GHz with an angular resolution ranging from 9.9 to 4.4 . The white noise level is around 1.5 μK degree or less in the 3 main CMB channels (100–217 GHz). The photometric accuracy is better than 2% at frequencies between 100 and 353 GHz and around 7% at the two highest frequencies. The maps created by the HFI Data Processing Centre reach our goals in terms of sensitivity, resolution, and photometric accuracy. They are already sufficiently accurate and well-characterised to allow scientific analyses which are presented in an accompanying series of early papers. At this stage, HFI data appears to be of high quality and we expect that with further refinements of the data processing we should be able to achieve, or exceed, the science goals of the Planck project

Planck 2013 results. I. Overview of products and scientific results

Peer reviewe

Planck early results. II. The thermal performance of Planck

The performance of the Planck instruments in space is enabled by their low operating temperatures, 20 K for LFI and 0.1 K for HFI, achieved through a combination of passive radiative cooling and three active mechanical coolers. The scientific requirement for very broad frequency coverage led to two detector technologies with widely different temperature and cooling needs. Active coolers could satisfy these needs; a helium cryostat, as used by previous cryogenic space missions (IRAS, COBE, ISO, Spitzer, AKARI), could not. Radiative cooling is provided by three V-groove radiators and a large telescope baffle. The active coolers are a hydrogen sorption cooler (<20 K), a 4He Joule-Thomson cooler (4.7 K), and a 3He-4He dilution cooler (1.4 K and 0.1 K). The flight system was at ambient temperature at launch and cooled in space to operating conditions. The HFI bolometer plate reached 93 mK on 3 July 2009, 50 days after launch. The solar panel always faces the Sun, shadowing the rest of Planck, and operates at a mean temperature of 384 K. At the other end of the spacecraft, the telescope baffle operates at 42.3 K and the telescope primary mirror operates at 35.9 K. The temperatures of key parts of the instruments are stabilized by both active and passive methods. Temperature fluctuations are driven by changes in the distance from the Sun, sorption cooler cycling and fluctuations in gas-liquid flow, and fluctuations in cosmic ray flux on the dilution and bolometer plates. These fluctuations do not compromise the science data

Planck 2013 results. XVII. Gravitational lensing by large-scale structure

On the arcminute angular scales probed by Planck, the cosmic microwave background (CMB) anisotropies are gently perturbed by gravitational lensing. Here we present a detailed study of this effect, detecting lensing independently in the 100, 143, and 217 GHz frequency bands with an overall significance of greater than 25σ. We use thetemperature-gradient correlations induced by lensing to reconstruct a (noisy) map of the CMB lensing potential, which provides an integrated measure of the mass distribution back to the CMB last-scattering surface. Our lensing potential map is significantly correlated with other tracers of mass, a fact which we demonstrate using several representative tracers of large-scale structure. We estimate the power spectrum of the lensing potential, finding generally good agreement with expectations from the best-fitting ΛCDM model for the Planck temperature power spectrum, showing that this measurement at z = 1100 correctly predicts the properties of the lower-redshift, later-time structures which source the lensing potential. When combined with the temperature power spectrum, our measurement provides degeneracy-breaking power for parameter constraints; it improves CMB-alone constraints on curvature by a factor of two and also partly breaks the degeneracy between the amplitude of the primordial perturbation power spectrum and the optical depth to reionization, allowing a measurement of the optical depth to reionization which is independent of large-scale polarization data. Discarding scale information, our measurement corresponds to a 4% constraint on the amplitude of the lensing potential power spectrum, or a 2% constraint on the root-mean-squared amplitude of matter fluctuations at z ~ 2

Planck early results. VI. The High Frequency Instrument data processing

We describe the processing of the 336 billion raw data samples from the High Frequency Instrument (HFI) which we performed to produce six temperature maps from the first 295 days of Planck-HFI survey data. These maps provide an accurate rendition of the sky emission at 100, 143, 217, 353, 545 and 857 GHz with an angular resolution ranging from 9.9 to 4.4^2. The white noise level is around 1.5 {\mu}K degree or less in the 3 main CMB channels (100--217GHz). The photometric accuracy is better than 2% at frequencies between 100 and 353 GHz and around 7% at the two highest frequencies. The maps created by the HFI Data Processing Centre reach our goals in terms of sensitivity, resolution, and photometric accuracy. They are already sufficiently accurate and well-characterised to allow scientific analyses which are presented in an accompanying series of early papers. At this stage, HFI data appears to be of high quality and we expect that with further refinements of the data processing we should be able to achieve, or exceed, the science goals of the Planck project.Comment: Replaced by the accepted version for publication, as part of a package of papers describing first results of the Planck mission The paper with figures at full resolution and full color tables can also be downloaded from the ESA site http://www.rssd.esa.int/Planc

Planck early results: first assessment of the High Frequency Instrument in-flight performance

The Planck High Frequency Instrument (HFI) is designed to measure the temperature and polarization anisotropies of the Cosmic Microwave Background and galactic foregrounds in six wide bands centered at 100, 143, 217, 353, 545 and 857 GHz at an angular resolution of 10' (100 GHz), 7' (143 GHz), and 5' (217 GHz and higher). HFI has been operating flawlessly since launch on 14 May 2009. The bolometers cooled to 100 mK as planned. The settings of the readout electronics, such as the bolometer bias current, that optimize HFI's noise performance on orbit are nearly the same as the ones chosen during ground testing. Observations of Mars, Jupiter, and Saturn verified both the optical system and the time response of the detection chains. The optical beams are close to predictions from physical optics modeling. The time response of the detection chains is close to pre-launch measurements. The detectors suffer from an unexpected high flux of cosmic rays related to low solar activity. Due to the redundancy of Planck's observations strategy, the removal of a few percent of data contaminated by glitches does not affect significantly the sensitivity. The cosmic rays heat up significantly the bolometer plate and the modulation on periods of days to months of the heat load creates a common drift of all bolometer signals which do not affect the scientific capabilities. Only the high energy cosmic rays showers induce inhomogeneous heating which is a probable source of low frequency noise.Comment: Submitted to A&A. 22 pages, 6 tables, 21 figures. One of a set of simultaneous papers for the Planck Missio

The spread of a financial virus through Europe and beyond

We analyse the importance of international relations between countries on the financial stability. The contagion effect in the network is tested by implementing an epidemiological model, comprising a number of European countries and using bilateral data on foreign claims between them. Banking statistics of consolidated foreign claims on ultimate risk bases, obtained from the Banks of International Settlements, allow us to measure the exposure of contagion spreading from a particular country to the other national banking systems. We show that the financial system of some countries, experiencing the debt crisis, is a source of global systemic risk because they threaten the stability of a larger system, being a global threat to the intoxication of the world economy and resulting in what we call a `financial virus'. Illustrative simulations were done in the NetLogo multi-agent programmable modelling environment and in MATLAB.publishe