54 research outputs found
El nuevo Geoportal de la Diputación de Badajoz: las TIG al servicio del ciudadano
El ciudadano cada vez goza de un mayor protagonismo, que viene motivado fundamentalmente por su ansias de saber, de conocer, y en una gran medida de participar, de colaborar y de sentirse parte de la sociedad en la que vive .No ajenos a esta realidad, desde el Servicio de Información Geográfica de la Diputación de Badajoz nos adentramos, hace ocho meses, en un proyecto ilusionante, de cambio radical en nuestra forma de ver, de presentar y de acercar la información al ciudadano, y por supuesto de hacerle partícipe de lo que ocurre a su alrededor
Integration of RNA-Seq data with heterogeneous microarray data for breast cancer profiling
Background: Nowadays, many public repositories containing large microarray gene expression datasets are
available. However, the problem lies in the fact that microarray technology are less powerful and accurate than more
recent Next Generation Sequencing technologies, such as RNA-Seq. In any case, information from microarrays is
truthful and robust, thus it can be exploited through the integration of microarray data with RNA-Seq data.
Additionally, information extraction and acquisition of large number of samples in RNA-Seq still entails very high costs
in terms of time and computational resources.This paper proposes a new model to find the gene signature of breast
cancer cell lines through the integration of heterogeneous data from different breast cancer datasets, obtained from
microarray and RNA-Seq technologies. Consequently, data integration is expected to provide a more robust statistical
significance to the results obtained. Finally, a classification method is proposed in order to test the robustness of the
Differentially Expressed Genes when unseen data is presented for diagnosis.
Results: The proposed data integration allows analyzing gene expression samples coming from different
technologies. The most significant genes of the whole integrated data were obtained through the intersection of the
three gene sets, corresponding to the identified expressed genes within the microarray data itself, within the RNA-Seq
data itself, and within the integrated data from both technologies. This intersection reveals 98 possible
technology-independent biomarkers. Two different heterogeneous datasets were distinguished for the classification
tasks: a training dataset for gene expression identification and classifier validation, and a test dataset with unseen data
for testing the classifier. Both of them achieved great classification accuracies, therefore confirming the validity of the
obtained set of genes as possible biomarkers for breast cancer. Through a feature selection process, a final small
subset made up by six genes was considered for breast cancer diagnosis.
Conclusions: This work proposes a novel data integration stage in the traditional gene expression analysis pipeline
through the combination of heterogeneous data from microarrays and RNA-Seq technologies. Available samples
have been successfully classified using a subset of six genes obtained by a feature selection method. Consequently, a
new classification and diagnosis tool was built and its performance was validated using previously unseen samples.This work was supported by Project TIN2015-71873-R (Spanish Ministry of
Economy and Competitiveness -MINECO- and the European Regional
Development Fund -ERDF)
Leukemia multiclass assessment and classification from Microarray and RNA-seq technologies integration at gene expression level
In more recent years, a significant increase in the number of available biological experiments
has taken place due to the widespread use of massive sequencing data. Furthermore,
the continuous developments in the machine learning and in the high performance
computing areas, are allowing a faster and more efficient analysis and processing of this
type of data. However, biological information about a certain disease is normally widespread
due to the use of different sequencing technologies and different manufacturers, in different
experiments along the years around the world. Thus, nowadays it is of paramount importance
to attain a correct integration of biologically-related data in order to achieve genuine
benefits from them. For this purpose, this work presents an integration of multiple Microarray
and RNA-seq platforms, which has led to the design of a multiclass study by collecting samples
from the main four types of leukemia, quantified at gene expression. Subsequently, in
order to find a set of differentially expressed genes with the highest discernment capability
among different types of leukemia, an innovative parameter referred to as coverage is presented
here. This parameter allows assessing the number of different pathologies that a
certain gen is able to discern. It has been evaluated together with other widely known
parameters under assessment of an ANOVA statistical test which corroborated its filtering
power when the identified genes are subjected to a machine learning process at multiclass
level. The optimal tuning of gene extraction evaluated parameters by means of this statistical
test led to the selection of 42 highly relevant expressed genes. By the use of minimum-
Redundancy Maximum-Relevance (mRMR) feature selection algorithm, these genes were
reordered and assessed under the operation of four different classification techniques. Outstanding
results were achieved by taking exclusively the first ten genes of the ranking into
consideration. Finally, specific literature was consulted on this last subset of genes, revealing
the occurrence of practically all of them with biological processes related to leukemia. At sight of these results, this study underlines the relevance of considering a new parameter
which facilitates the identification of highly valid expressed genes for simultaneously discerning
multiple types of leukemia.This work was supported by Project
TIN2015-71873-R (Spanish Ministry of Economy
and Competitiveness -MINECO- and the European Regional Development Fund -ERDF) and Junta de
Andalucı´a (P12–TIC–2082)
POR UNA CULTURA DE PAZ: UNA MIRADA DESDE LAS CIENCIAS DE LA CONDUCTA
En
virtud
de
lo
anterior,
los
estudiosos
de
las
ciencias
de
la
conducta
de
la
Universidad
Autónoma
del
Estado
de
México,
ante
la
persistencia
y
proliferación
de
estos
hechos
en
diversas
partes
del
Mundo
y
de
nuestro
país
en
particular, se
convocó
a
los
estudiosos
interesados
y
a
la
sociedad
en
general
a
presentar
trabajos
para
analizar,
debatir
y
proponer
estrategias
de
acción
y
dirección,
que
fortalezcan
una
convivencia y bienestar con sentido humanista para una cultura de paz.
El
presente
texto
es
producto
de
esta convocatoria
que
recoge
los
trabajos
de
los
interesados
en
la
temática,
de
diferentes
países
(España,
Argentina,
Cuba,
Brasil,
Costa
Rica
y
México)
retomando
con
ello
sus
experiencias
relativas
al
estudio,
análisis,
comprensión
e
instrumentación
de
la
cultura
de
paz
en
los
distintos
ámbitos
institucionales
en
los
que
participan:
educativo,
salud,
penitenciario,
social,
laboral,
familia,
alimentario,
psicológico,
por
mencionar
algunos.
El
presente
libro,
propicia
un
espacio
de
reflexión,
diálogo
y
posicionamiento
de
las
ciencias
de
la
conducta
para
la
apropiación,
análisis,
debate
y
propuestas
que
fortalezcan
una
cultura
de
paz
a
través
de
la
convivencia
y
el
bienestar
social
con
sentido
humanista.
El
sistema
económico
neoliberal
y
el
proceso
de
globalización
han
contribuido
al
logro
de
avances
significativos
en
la
ciencia
y
la
tecnología,
pero
también
han
propiciado
la
polarización
de
las
sociedades
lo
que
ha
impactado
de
manera
negativa
a
la
sociedad
en
su
conjunto,
pero
en
mayor
medida
a
los grupos
vulnerables. Dicha
polarización
ha
traído
consigo
un
desarrollo
desigual
del
mundo
que
se
expresa
de
diferentes
maneras
tanto
en
países
desarrollados
como
en
los
llamados
del
tercer
mundo,
en
donde
no
están
satisfechas
las
necesidades
humanas
elementales
de
todos
los
sectores
de
la
población,
siempre
falta
algo.
Si
a
esto
le
sumamos
los
conflictos
internacionales por
diferentes
motivos
que
enfrentan
algunas
naciones,
una
insuficiente
cobertura
educativa
y
de
salud,
desempleo
y
pobreza
extrema,
entre
otras
cosas;
estamos
frente
a
retos
de
gran
envergadura
para
los
gobiernos,
para
los
estudiosos
y
para
la
sociedad
civil
en
general. Uno
de
los
intentos
para
frenar
y prevenir
la
agudización
de
estas
problemáticas
es
la
cultura
de
paz,
cuyo
estudio
y propuestas
han
ido
avanzando
en
diferentes
sentidos
y
de
manera
favorable,
el
tema
está
presente
en
diferentes
Organismos
Internacionales
como
la
ONU,
la
UNESCO,
la
OCDE,
El
Banco
Mundial,
entre
otros.
Pero
falta
mucho
por
hacer.Universidad Autónoma del Estado de Méxic
Conferencia internacional. Desafíos de la Investigación e innovación en tiempos de Covid-19
En 2018, Treinta y nueve estudiantes femeninas del X ciclo del periodo
2018-1 de la escuela de Psicología de una universidad particular del cono
norte de Lima, ellas han descrito el romance de su personas cercanas a
ellas. Se analizaron los 266 casos de parejas de enamorados estaban el
rango de 17 a 25 años. El instrumento que se usó fue una CUESTIONARIO
CUPIDO que es una escala nominal de conductas donde las estudiantes de
psicología clasificaban los comportamientos en categorías preestablecidas,
claramente definidas y mutuamente excluyentes. La conclusión más
importante del estudio observacional fue que las parejas analizadas en un
64.4 % la mujer ero atrajo al varón y después el varón emo conectó con
la mujer. También han observado otro estilo de romance cuando el varón
ero atrajo a la mujer por su cuerpo atracción y después la mujer fue la que
emo conectó con el varón y observaron este hecho en un 35.6 % de los
casos
O mercado da identidade. O nativismo entre o colecionismo e as galerias durante o século XX
El presente artículo indagará en la presencia y circulación del nativismo en el mercado de arte argentino, entre 1910 y 1970. Para ello, realizaré una reconstrucción del contexto de surgimiento del nativismo como campo temático y, posteriormente, abordaré dos casos que se constituyen como paradigmas de su supervivencia: la trayectoria de Jorge Bermúdez (uno de los primeros artistas nativistas en alcanzar la consagración) y la obra de Antonio Berni (que circuló por el mercado durante la segunda mitad del siglo). Como hipótesis general, sostengo que la prolongada vida del nativismo se correspondió con una presencia constante de estas obras en colecciones y galerías privadas.This paper will inquire the presence and circulation of nativism in the Argentine art market between 1910 and 1970. To that end, I will first reconstruct the context of emergence of nativism as a thematic field and, later, I will address two cases which constitute paradigms of survival—the trajectory of Jorge Bermúdez (one of the first nativist artists to achieve success) and the work of Antonio Berni (which circulated through the market during the second half of the century). As a general hypothesis, I will maintain that the prolonged life of nativism was accompanied by a constant presence of these works in private collections and galleries.O presente artigo investigará a presença e a circulação do nativismo no mercado da arte argentino, entre 1910 e 1970. Para tanto, realizarei uma reconstrução do contexto de surgimento do nativismo como campo temático e, posteriormente, ocupar-me-ei de dois casos que se constituem como paradigmas da sua sobrevivência: a trajetória de Jorge Bermúdez (um dos primeiros artistas nativistas que alcançaram consagração) e a obra de Antonio Berni (que circulou pelo mercado durante a segunda metade do século). Como hipótese geral, sustento que a prolongada vida do nativismo corresponde a uma presença constante dessas obras em coleções e galerias privadas.Fil: Fasce, Pablo Javier. Universidad Nacional de San Martín. Instituto de Altos Estudios Sociales; Argentina. Universidad Nacional de San Martín. Centro de Investigaciones en Arte y Patrimonio. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Arte y Patrimonio; Argentin
Treatment with tocilizumab or corticosteroids for COVID-19 patients with hyperinflammatory state: a multicentre cohort study (SAM-COVID-19)
Objectives: The objective of this study was to estimate the association between tocilizumab or corticosteroids and the risk of intubation or death in patients with coronavirus disease 19 (COVID-19) with a hyperinflammatory state according to clinical and laboratory parameters.
Methods: A cohort study was performed in 60 Spanish hospitals including 778 patients with COVID-19 and clinical and laboratory data indicative of a hyperinflammatory state. Treatment was mainly with tocilizumab, an intermediate-high dose of corticosteroids (IHDC), a pulse dose of corticosteroids (PDC), combination therapy, or no treatment. Primary outcome was intubation or death; follow-up was 21 days. Propensity score-adjusted estimations using Cox regression (logistic regression if needed) were calculated. Propensity scores were used as confounders, matching variables and for the inverse probability of treatment weights (IPTWs).
Results: In all, 88, 117, 78 and 151 patients treated with tocilizumab, IHDC, PDC, and combination therapy, respectively, were compared with 344 untreated patients. The primary endpoint occurred in 10 (11.4%), 27 (23.1%), 12 (15.4%), 40 (25.6%) and 69 (21.1%), respectively. The IPTW-based hazard ratios (odds ratio for combination therapy) for the primary endpoint were 0.32 (95%CI 0.22-0.47; p < 0.001) for tocilizumab, 0.82 (0.71-1.30; p 0.82) for IHDC, 0.61 (0.43-0.86; p 0.006) for PDC, and 1.17 (0.86-1.58; p 0.30) for combination therapy. Other applications of the propensity score provided similar results, but were not significant for PDC. Tocilizumab was also associated with lower hazard of death alone in IPTW analysis (0.07; 0.02-0.17; p < 0.001).
Conclusions: Tocilizumab might be useful in COVID-19 patients with a hyperinflammatory state and should be prioritized for randomized trials in this situatio
re-habitar El Carmen : Un proyecto sobre patrimonio contemporáneo
El proyecto _re-HABITAR suponía para el propio proceder de la institución un avance más allá del reconocimiento, registro, inventario o protección patrimonial de la arquitectura del siglo XX y del Movimiento Moderno para posicionarse en la acción preventiva y conservativa de ese legado contemporáneo. Para ello, la praxis patrimonial se aferraba a un modelo: el de la vivienda social en España en la segunda mitad del siglo XX; a un caso concreto: el de la barriada de Nuestra Señora del Carmen (Recasens Méndez-Queipo de Llano, 1958); y a un requisito fundamental: analizar un objeto vivo y en uso, aún con la presencia de quienes lo vivieron y usaron desde su origen
Use of bioceramics enhanced with effective microorganisms as an additive for construction: study of physical and mechanical properties in cement mortars and gypsum plasters
Biomaterials are materials that are used to manufacture devices that interact with biological systems. According to their chemical composition, they can be classified as biometals, biopolymers, bioceramics, biocomposites and semiconductors. Thus, in the present work, the application of bioceramics, enhanced with effective microorganisms, to construction materials (cement mortars and gypsum plasters) was studied in order to see the benefits that its incorporation contributes to construction materials. This first work constitutes the first phase of an experimental campaign in which the influence of bioceramics on the physical and mechanical properties (flexural and compressive strength) of the studied materials was analyzed. Furthermore, scanning electron microscopy (SEM) and mercury intrusion porosimetry (MIP) techniques were used. According to the results, a slight improvement in the mechanical properties of the new composites was observed. Besides, a more compact matrix was observed when bioceramics were used as an aggregate to the mixtures
KnowSeq R-Bioc package: The automatic smart gene expression tool for retrieving relevant biological knowledge
This work was funded by the Spanish Ministry of Sciences, Innovation and Universities under Project RTI2018-101674-B-I00 titled "Computer Architectures and Machine Learningbased solutions for complex challenges in Bioinformatics, Biotechnology and Biomedicine", in collaboration with the Government of Andalusia under Postdoctoral Grant P12TIC2082. The funders had no role in study design, datacollection and analysis, decision to publish, or preparation of this manuscript. The results published here are in whole or part based upon data generated by the TCGA Research Network: https:// www.cancer. gov/tcga.KnowSeq R/Bioc package is designed as a powerful, scalable and modular software focused on automatizing and assembling renowned bioinformatic tools with new features and functionalities. It comprises a unified environment to perform complex gene expression analyses, covering all the needed processing steps to identify a gene signature for a specific disease to gather understandable knowledge. This process may be initiated from raw files either available at well-known platforms or provided by the users themselves, and in either case coming from different information sources and different Transcriptomic technologies. The pipeline makes use of a set of advanced algorithms, including the adaptation of a novel procedure for the selection of the most representative genes in a given multiclass problem. Similarly, an intelligent system able to classify new patients, providing the user the opportunity to choose one among a number of well-known and widespread classification and feature selection methods in Bioinformatics, is embedded. Furthermore, KnowSeq is engineered to automatically develop a complete and detailed HTML report of the whole process which is also modular and scalable. Biclass breast cancer and multiclass lung cancer study cases were addressed to rigorously assess the usability and efficiency of KnowSeq. The models built by using the Differential Expressed Genes achieved from both experiments reach high classification rates. Furthermore, biological knowledge was extracted in terms of Gene Ontologies, Pathways and related diseases with the aim of helping the expert in the decision-making process. KnowSeq is available at Bioconductor (https://bioconductor.org/packages/KnowSeq), GitHub (https://github.com/CasedUgr/KnowSeq) and Docker (https://hub.docker.com/r/casedugr/knowseq).Spanish Ministry of Sciences, Innovation and Universities RTI2018-101674-B-I00Government of Andalusia P12TIC208
- …