Otter spotter: The Creation and evaluation of an educational resource for the association of zoos and aquariums, Rochester Institute of Technology, and the Seneca Park Zoo

Otter Spotter is an educational website providing information about otters found in Association of Zoos and Aquariums\u27 (AZA) facilities. Though the site includes five species of otter it focuses primarily on the North American river otter. It was designed to meet the needs of AZA\u27s Otter Species Survival Plan (SSP) committee, Rochester Institute of Technology\u27s (RIT) Otter Research group, and the Seneca Park Zoo. This site helps fulfill the mission of modern zoos to become centers for conservation and education. Otter Spotter contains species information, photos, video clips, research summaries, teacher resources, and a blog with the latest news related to otters. Site statistics show that Otter Spotter is being used frequently with an increase in page views when social media tools are utilized. Evaluations show that the site is appealing and easy to navigate, useful to AZA educators, and will continue to be used as a resource for AZA facilities. Pre and post-testing of the lesson plans designed for the site also indicate that the lesson plans are a useful way to teach kindergarten through fourth grade students about otters and meet the standards of the New York State Core Curriculum. Overall, the site is a successful resource and by utilizing the findings of the evaluations it will continue to grow and extend its reach beyond the AZA community

Molecular Insights into Prostate Field Cancerization: Telomere Length, EGR-­‐1 Expression, and Regulation of MIC-­‐1, PDGF-­‐A, and FAS

The diagnosis of prostate cancer (adenocarcinoma) relies on screening for elevated prostate-specific antigen (PSA) in blood samples and on digital rectal examination (DRE). With high PSA levels and/or abnormal DRE, physicians recommend a biopsy, which often misses the location of the adenocarcinoma and results in false negatives. Previous studies have shown expression of the key transcription factor early growth response 1 (EGR-1), the pro-survival factor macrophage inhibitor cytokine 1 (MIC-1), and the growth stimulatory platelet derived growth factor A (PDGF-A) to be up-regulated in histologically normal tissues 1 centimeter adjacent to prostate adenocarcinomas. We hypothesize that tumors emerge from “field cancerized” tissues, and while such tissues appear normal under gross histological examination, further analysis reveals a molecular history that adds insight into development of oncogenesis. We aim to explore EGR-1, MIC-1, and PDGF-A as essential markers of field cancerization and prostate oncogenesis that may also provide an early detection of premalignant cells by biochemical and molecular biological methods. We present here data from our ongoing investigations into the effect of tissue microenvironmental factors involved in tumorigenesis, for example oxidative stress and telomere-mediated genomic instability in human prostate cancer cell models. We also present data on the regulation of expression of MIC-1 and PDGF-A by EGR-1 using human prostate cancer cell models

Ten years of lesbian health survey research in the UK West Midlands

Abstract Background Very little is known about the physical health needs of lesbian and bisexual women in the UK; most research has looked at mental or sexual health only. This article reports the results of four surveys carried out in the West Midlands between 1995 and 2005. Methods The first two surveys were conducted in 1995–6 by a volunteer group, with participants from a lesbian health conference (n = 69) and in a convenience sample from a wide range of relevant groups and venues (n = 354). The second two surveys were commissioned by the West Midlands South Strategic Health Authority in partnership with the Gay Men's Health Network and were conducted in 2002 (n = 449) and 2005 (n = 166) and again used convenience sampling methods including the internet. Results The mean age of respondents varied between 29–33 years and 5–7% were from a non-white ethnic background. The smoking rates varied from 42% o 55%, being twice the West Midlands regional average of 21% for women aged 16 or more. Similarly, problems with alcohol were reported in 25–37% of respondents, higher than the West Midlands regional average of 7% for women aged 16+. The prevalence of any mental health problem varied between 31–35% and any suicide attempt between 20–31%. Only 29–45% had revealed their sexual orientation to their GP and of these, approximately 50% had experienced a positive reaction. Conclusion The results suggest health needs that current UK health services may not be meeting. There is a need to identify and target specific health measures for lesbians and bisexual women in order to ensure improved physical and mental health in the longer term.</p

A many-analysts approach to the relation between religiosity and well-being

The relation between religiosity and well-being is one of the most researched topics in the psychology of religion, yet the directionality and robustness of the effect remains debated. Here, we adopted a many-analysts approach to assess the robustness of this relation based on a new cross-cultural dataset (N=10,535 participants from 24 countries). We recruited 120 analysis teams to investigate (1) whether religious people self-report higher well-being, and (2) whether the relation between religiosity and self-reported well-being depends on perceived cultural norms of religion (i.e., whether it is considered normal and desirable to be religious in a given country). In a two-stage procedure, the teams first created an analysis plan and then executed their planned analysis on the data. For the first research question, all but 3 teams reported positive effect sizes with credible/confidence intervals excluding zero (median reported β=0.120). For the second research question, this was the case for 65% of the teams (median reported β=0.039). While most teams applied (multilevel) linear regression models, there was considerable variability in the choice of items used to construct the independent variables, the dependent variable, and the included covariates

A Many-analysts Approach to the Relation Between Religiosity and Well-being

The relation between religiosity and well-being is one of the most researched topics in the psychology of religion, yet the directionality and robustness of the effect remains debated. Here, we adopted a many-analysts approach to assess the robustness of this relation based on a new cross-cultural dataset (N = 10, 535 participants from 24 countries). We recruited 120 analysis teams to investigate (1) whether religious people self-report higher well-being, and (2) whether the relation between religiosity and self-reported well-being depends on perceived cultural norms of religion (i.e., whether it is considered normal and desirable to be religious in a given country). In a two-stage procedure, the teams first created an analysis plan and then executed their planned analysis on the data. For the first research question, all but 3 teams reported positive effect sizes with credible/confidence intervals excluding zero (median reported β = 0.120). For the second research question, this was the case for 65% of the teams (median reported β = 0.039). While most teams applied (multilevel) linear regression models, there was considerable variability in the choice of items used to construct the independent variables, the dependent variable, and the included covariates

Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018.

Over the past decade, the Nomenclature Committee on Cell Death (NCCD) has formulated guidelines for the definition and interpretation of cell death from morphological, biochemical, and functional perspectives. Since the field continues to expand and novel mechanisms that orchestrate multiple cell death pathways are unveiled, we propose an updated classification of cell death subroutines focusing on mechanistic and essential (as opposed to correlative and dispensable) aspects of the process. As we provide molecularly oriented definitions of terms including intrinsic apoptosis, extrinsic apoptosis, mitochondrial permeability transition (MPT)-driven necrosis, necroptosis, ferroptosis, pyroptosis, parthanatos, entotic cell death, NETotic cell death, lysosome-dependent cell death, autophagy-dependent cell death, immunogenic cell death, cellular senescence, and mitotic catastrophe, we discuss the utility of neologisms that refer to highly specialized instances of these processes. The mission of the NCCD is to provide a widely accepted nomenclature on cell death in support of the continued development of the field

Molecular Insights into Prostate Cancerization: Telomere Length, EGR-1 Expression, and Regulation of MIC‐1, PDGF-A, and FAS

Detection of prostate cancer, while still confined to the prostate, has a good chance for successful treatment. High levels of prostate specific antigen and/or abnormal digital rectal antigen cause physicians to recommend a biopsy, which often misses the location of the adenocarcinoma and results in false negatives. Field cancerization denotes the occurrence of molecular alterations in structurally intact cells in histologically normal tissues surrounding tumors. Our research features complementary approaches towards revealing the importance of potential mediators of prostate field cancerization, such as early growth response 1 (EGR-1), fatty acid synthase (FAS), macrophage inhibitory cytokine 1 (MIC-1), and platelet derived growth factor A (PDGF-A). We explored the potential regulatory function of the transcription factor EGR-1 for FAS, MIC-1, and PDGF-A towards the identification of functional pathways of prostate field cancerization. This hypothesis was tested by over-expressing and down-regulating EGR-1 using recombinant DNA technology and determining the effect on FAS, MIC-1, and PDGF-A protein expression in human prostate cell models. A better understanding of such pathways will ultimately lead to an enhanced indication of cancer presence regardless of whether the biopsy cores contain cancerous tissue

Anterior Loculated Pericardial Effusion Misinterpreted as Right Heart Dilation Suggesting Pulmonary Embolism

We report a case of anterior loculated pericardial effusion misinterpreted on point-of-care ultrasound as a dilated right ventricle, and suggesting diagnosis of pulmonary embolism (PE), in a patient with renal failure. The compressed right ventricle from tamponade physiology appeared to be a thickened intraventricular septum. Heparin was given empirically for presumed PE. Later the same day, computed tomography of the chest showed the effusion, as did formal echocardiogram. The patient had drainage of 630 milliliters of fluid and recovered from tamponade. Loculated effusions comprise 15% of all pericardial effusions, and misdiagnosis of PE with heparin therapy could be fatal