Impact of the European Clinical Trials Directive on prospective academic clinical trials associated with BMT

The European Clinical Trials Directive (EU 2001; 2001/20/EC) was introduced to improve the efficiency of commercial and academic clinical trials. Concerns have been raised by interested organizations and institutions regarding the potential for negative impact of the Directive on non-commercial European clinical research. Interested researchers within the European Group for Blood and Marrow Transplantation (EBMT) were surveyed to determine whether researcher experiences confirmed this view. Following a pilot study, an internet-based questionnaire was distributed to individuals in key research positions in the European haemopoietic SCT community. Seventy-one usable questionnaires were returned from participants in different EU member states. The results indicate that the perceived impact of the European Clinical Trials Directive has been negative, at least in the research areas of interest to the EBMT

Consumer attitudes towards production diseases in intensive production systems

Many members of the public and important stakeholders operating at the upper end of the food chain, may be unfamiliar with how food is produced, including within modern animal production systems. The intensification of production is becoming increasingly common in modern farming. However, intensive systems are particularly susceptible to production diseases, with potentially negative consequences for farm animal welfare (FAW). Previous research has demonstrated that the public are concerned about FAW, yet there has been little research into attitudes towards production diseases, and their approval of interventions to reduce these. This research explores the public’s attitudes towards, and preferences for, FAW interventions in five European countries (Finland, Germany, Poland, Spain and the UK). An online survey was conducted for broilers (n = 789), layers (n = 790) and pigs (n = 751). Data were analysed by means of Kruskal-Wallis ANOVA, exploratory factor analysis and structural equation modelling. The results suggest that the public have concerns regarding intensive production systems, in relation to FAW, naturalness and the use of antibiotics. The most preferred interventions were the most “proactive” interventions, namely improved housing and hygiene measures. The least preferred interventions were medicine-based, which raised humane animal care and food safety concerns amongst respondents. The results highlighted the influence of the identified concerns, perceived risks and benefits on attitudes and subsequent behavioural intention, and the importance of supply chain stakeholders addressing these concerns in the subsequent communications with the public

Factors affecting consumers’ evaluation of food derived from animals fed insect meal: A systematic review

Data availability: Data will be made available on request.Supplementary data are available online at https://www.sciencedirect.com/science/article/pii/S0924224423001620?via%3Dihub#coi0010:~:text=Appendix%20A.-,Supplementary%20data,-Data%20availability .Copyright © 2023 The Authors. . Background: Research in recent years has shown there is high potential for various insect species to converting organic substrates into high-quality feedstuffs. Insect-derived meal is increasingly being used to replace conventional feedstuffs such as soybean and fishmeal in animal diets, due to its high protein and essential amino acid content. However, research on consumer acceptance of foods derived from animals produced using insect-based meal is fragmented. Scope and approach: A systematic literature review was carried out in Scopus, Web of Science, AgEcon, and Google Scholar, with a total of 28 articles meeting the selection criteria. Papers were reviewed to identify factors affecting consumer acceptance of insect-based feed. Key Findings and Conclusions: Overall, the review indicated that insects are more acceptable to consumers as an animal feedstuff than as human food. The most important factors influencing consumer evaluation of insect-based feed included risk perceptions, knowledge and heuristic cues. Ethical and environmental concerns also played a role in the decision-making process, but their effect on consumer acceptance of using insects in animal feed was less important. Food neophobia, disgust and uncertainties about safety and health, mainly deriving from lack of knowledge on the part of consumers, emerged as critical barriers to acceptance of insects in animal feed. Greater familiarity with the technology used for the production of insect meals could alleviate disgust and even lessen the impact of neophobia, although affective emotional reactions are unlikely to be changed by awareness and provision of information alone. Technological issues (including substrates and insect species) as well as price changes in the resulting animal-based foods are relevant factors that warrant further research in relation to consumer acceptance

SYNTHETIC BIOLOGY APPLIED IN THE AGRIFOOD SECTOR: SOCIETAL PRIORITIES AND PITFALLS

Synthetic biology offers potential for innovation in the agrifood sector, although concerns have been raised consumer rejection of applications will occur similar to that associated with the introduction of genetically modified foods. Risk-benefit assessment should address socio-economic, as well as health and environmental impacts. Ethical issues may be of particular relevance to the application synthetic biology, and may also resonate with societal concerns. A case-by-case analysis of relevant issues may be needed, and innovation must be driven by societal and consumer preferences as well as technological possibilities. Research into consumer and societal priorities is required early in the innovation trajector

Willingness to pay for personalised nutrition across Europe

yesPersonalised nutrition (PN) may promote public health. PN involves dietary advice based on individual characteristics of end users and can for example be based on lifestyle, blood and/or DNA profiling. Currently, PN is not refunded by most health insurance or health care plans. Improved public health is contingent on individual consumers being willing to pay for the service. Methods: A survey with a representative sample from the general population was conducted in eight European countries (N = 8233). Participants reported their will- 25 ingness to pay (WTP) for PN based on lifestyle information, lifestyle and blood information, and lifestyle and DNA information. WTP was elicited by contingent valuation with the price of a standard, non-PN advice used as reference. Results: About 30% of participants reported being willing to pay more for PN than for non-PN advice. They were on average prepared to pay about 150% of the reference price of a standard, non-personalised advice, with some differences related to socio-demographic factors. Conclusion: There is a potential market for PN 30 compared to non-PN advice, particularly among men on higher incomes. These findings raise questions to what extent personalized nutrition can be left to the market or should be incorporated into public health programsEC (FW7) funded Food4me projectThe full text will be available 12 months after publicatio

Sex hormone-regulated CMG2 is involved in breast and prostate cancer progression

Background/Aim: Capillary morphogenesis gene 2 (CMG2) is involved in prostate and breast cancer progression. This study aimed to investigate sex hormone receptor-mediated regulation of CMG2 in breast and prostate cancer, and its implication in disease progression. Materials and Methods: Expression of CMG2, oestrogen receptor (ER) and androgen receptor (AR) was determined in breast and prostate cancer cell lines, respectively, using real-time quantitative PCR (QPCR) and western blot. Association between CMG2 and sex hormone receptors was analysed in a number of transcriptome datasets. Immunochemical staining was performed in tissue microarrays of breast cancer (BR1505D) and prostate cancer (PR8011A). CMG2 expression was determined in 17β-oestradiol treated breast cancer cells and AR over-expressing prostate cancer cells. Results: CMG2 was found to be inversely correlated with sex hormone receptors in breast and prostate cancer. Lower expression of CMG2 was associated with a poor prognosis in ER (+) breast cancer but not ER (−) tumours. Both ER (+) breast cancer cell lines and AR (+) prostate cancer cell lines presented lower expression of CMG2, which was increased following sex hormone deprivation. Exposure to 17-β-oestradiol and AR over-expression repressed CMG2 expression in breast cancer and prostate cancer cell lines, respectively. Conclusion: CMG2 is inversely correlated with ER and AR status in breast and prostate cancer, respectively. ER and AR mediate repression of CMG2 expression in corresponding cancerous cells

Sex hormone-regulatedcmg2is involved in breast and prostate cancer progression

Background/Aim: Capillary morphogenesis gene 2 (CMG2) is involved in prostate and breast cancer progression. This study aimed to investigate sex hormone receptor-mediated regulation of CMG2 in breast and prostate cancer, and its implication in disease progression. Materials and Methods: Expression of CMG2, oestrogen receptor (ER) and androgen receptor (AR) was determined in breast and prostate cancer cell lines, respectively, using real-time quantitative PCR (QPCR) and western blot. Association between CMG2 and sex hormone receptors was analysed in a number of transcriptome datasets. Immunochemical staining was performed in tissue microarrays of breast cancer (BR1505D) and prostate cancer (PR8011A). CMG2 expression was determined in 17β-oestradiol treated breast cancer cells and AR over-expressing prostate cancer cells. Results: CMG2 was found to be inversely correlated with sex hormone receptors in breast and prostate cancer. Lower expression of CMG2 was associated with a poor prognosis in ER (+) breast cancer but not ER (−) tumours. Both ER (+) breast cancer cell lines and AR (+) prostate cancer cell lines presented lower expression of CMG2, which was increased following sex hormone deprivation. Exposure to 17-β-oestradiol and AR over-expression repressed CMG2 expression in breast cancer and prostate cancer cell lines, respectively. Conclusion: CMG2 is inversely correlated with ER and AR status in breast and prostate cancer, respectively. ER and AR mediate repression of CMG2 expression in corresponding cancerous cells

Potential implication of IL-24 in lymphangiogenesis of human breast cancer

Lymphangiogenesis is involved in the dissemination of malignant cells from solid tumours to regional lymph nodes and possibly to various distant sites. Lymphangiogenesis is regulated by vascular endothelial growth factor (VEGF)-C and VEGF-D. Interleukin (IL)-24 is known as a cytokine with potent antitumour and tumour-suppressive activity which functions through its receptor (IL-22R). Expression of IL-24 has been shown to be reduced in breast cancer, and the reduced expression is associated with lymphatic metastases and a poor prognosis. However, the involvement of IL-24 in lymphangiogenesis during lymphatic metastasis remains unclear. The aim of the present study was to determine whether there is an association between IL-24, IL-22R and lymphangiogenic factors and markers in breast cancer. Analysis of IL-24, IL-22R and lymphangiogenic factors in malignant breast tissue samples (n=127) revealed a correlation between increased expression of lymphangiogenic markers (podoplanin, Prox-1 and LYVE-1) and reduced levels of IL-24 and IL-22R. Samples stained with a high degree of positivity for lymphangiogenic factors and markers whereas staining for IL-24 was weak. In vitro assays showed that the average perimeter length of microtubules formed by endothelial cells treated with IL-24 was significantly reduced compared to the control. The growth of endothelial cells was significantly reduced when exposed to a high concentration of IL-24 (250 ng/ml). Treatment of HECV cells with IL-24 resulted in significantly reduced expression of VEGF-C (P<0.05) and VEGF-D (P<0.001). In conclusion, reduced expression of IL-24 and IL-22R in breast cancer is correlated with increased expression of specific lymphangiogenic markers. IL-24 suppressed in vitro growth and microtubule formation of endothelial cells. IL-24 may downregulate the expression of lymphangiogenic markers and factors although further research is required. This suggests that IL-24 plays a profound role in suppressing tumour lymphangiogenesis, thereby, reducing the likelihood of cancer metastasis via the lymphatic route

Percepção do consumidor frente aos riscos associados aos alimentos, sua segurança e rastreabilidade.

An increase in consumer interest in food safety has been observed in the last few decades. Governments have been pressed to adopt measures that assure the safety of foods and, as a consequence, legislation related to the contamination of food by physical, chemical and microbiological contaminants is being implemented. Within this context it is relevant to investigate the perception of Brazilian consumers on such issues. The present work evaluated the criteria used by consumers from Rio de Janeiro and Campinas to buy foods, and their vision of the risks of food contamination. The perception of consumers on traceability and its importance in contributing to the safety of foods was also evaluated using the Focus Group methodology. Three sessions were carried out (two in Campinas and the third in Rio de Janeiro). Differences were found between the consumers from the two cities. The Campinas consumers were more concerned and interested in the matter than those from Rio de Janeiro, since they reported looking for more information on the food labels. Mention was made of the risks of food poisoning, botulism, and pesticides by respondents in both cities. The main products considered as showing health risks were meats and seafood, the latter particularly in Rio. Products considered safe by consumers included fruits (without pesticides), vegetables and dried products. All the consumer groups were concerned about food contamination by pesticides and heavy metals. In relation to microbiological contamination, the three groups highlighted the risks of bacteria, especially Salmonella. Traceability was considered relevant by the three groups, since it should contribute to a more efficient product recall if necessary. However, from the consumer perspective, it will also lead to a price increase, although increasing consumer confidence in the safety of the foods. Many of the consumer concerns reflected information published in magazines and newspapers, such as the use of hormones in chicken. Industries and retailers should communicate scientific information about safety topics, thus contributing to consumer education.Nas últimas décadas, tem-se observado um crescente interesse dos consumidores pelas questões relacionadas à segurança dos alimentos. Os governos têm sido pressionados a adotar medidas que assegurem a inocuidade dos alimentos e, consequentemente, legislações rigorosas quanto à contaminação dos alimentos por agentes físicos, químicos e microbiológicos estão sendo implantadas. Dentro desse panorama, conhecer a percepção do consumidor brasileiro sobre o tema é extremamente relevante. No presente estudo, avaliaram-se, em duas grandes cidades brasileiras (Campinas-SP e Rio de Janeiro-RJ), os critérios utilizados por consumidores para a compra de produtos alimentícios e suas visões sobre os riscos potenciais de contaminação dos alimentos. Avaliou-se também a percepção dos consumidores sobre a rastreabilidade e seu papel na melhoria da segurança dos alimentos, utilizando-se a metodologia focus group. Três sessões de discussão foram conduzidas (duas em Campinas e uma no Rio de Janeiro), tendo sido observadas diferenças entre os consumidores das distintas praças. Os consumidores de Campinas mostraram-se mais preocupados e interessados no assunto do que os do Rio de Janeiro, pois relataram buscar mais informações nos rótulos de alimentos. Menções relacionadas a riscos, como intoxicação alimentar, botulismo e agrotóxicos foram comuns para os entrevistados das duas cidades e, entre os principais produtos considerados com risco à saúde, ficaram as carnes e os frutos do mar, sendo estes últimos com maior ênfase no Rio de Janeiro. Dentre os produtos reconhecidos como seguros, destacaram-se as frutas (produzidas sem agrotóxicos), os legumes e os produtos secos/desidratados. Em todos os grupos, observou-se grande preocupação com a contaminação por agrotóxicos e metais pesados. Em termos microbiológicos, ambas as praças destacaram os riscos com bactérias, especialmente a Salmonella. O tema rastreabilidade se mostrou relevante nos três grupos, pois esta deve contribuir para o recolhimento mais eficiente de produtos, caso seja necessário. No entanto, sob a ótica do consumidor, a rastreabilidade leva ao aumento dos preços, embora aumente a confiança em relação à segurança dos alimentos. Muitas das preocupações dos consumidores refletem as informações publicadas em revistas e jornais, como o caso da crença de que hormônios são utilizados na cadeia da carne de frango. Indústria e varejistas devem incrementar a comunicação de informações científicas sobre a segurança dos alimentos, contribuindo, assim, na educação do consumidor

Psychological Determinants of Consumer Acceptance of Personalised Nutrition in 9 European Countries

YesObjective: To develop a model of the psychological factors which predict people’s intention to adopt personalised nutrition. Potential determinants of adoption included perceived risk and benefit, perceived self-efficacy, internal locus of control and health commitment. Methods: A questionnaire, developed from exploratory study data and the existing theoretical literature, and including validated psychological scales was administered to N = 9381 participants from 9 European countries (Germany, Greece, Ireland, Poland, Portugal, Spain, the Netherlands, the UK, and Norway). Results: Structural equation modelling indicated that the greater participants’ perceived benefits to be associated with personalised nutrition, the more positive their attitudes were towards personalised nutrition, and the greater their intention to adopt it. Higher levels of nutrition self-efficacy were related to more positive attitudes towards, and a greater expressed intention to adopt, personalised nutrition. Other constructs positively impacting attitudes towards personalised nutrition included more positive perceptions of the efficacy of regulatory control to protect consumers (e.g. in relation to personal data protection), higher self-reported internal health locus of control, and health commitment. Although higher perceived risk had a negative relationship with attitude and an inverse relationship with perceived benefit, its effects on attitude and intention to adopt personalised nutrition was less influential than perceived benefit. The model was stable across the different European countries, suggesting that psychological factors determining adoption of personalised nutrition have generic applicability across different European countries. Conclusion: The results suggest that transparent provision of information about potential benefits, and protection of consumers’ personal data is important for adoption, delivery of public health benefits, and commercialisation of personalised nutrition.This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement n u 265494 (http://cordis.europa.eu/fp7/home_en.html). Food4Me is the acronym of the project ‘‘Personalised nutrition: an integrated analysis of opportunities and challenges’’ (http://www.food4me.org/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript