Microbial competition in porous environments can select against rapid biofilm growth

Microbes often live in dense communities called biofilms where competition between strains and species is fundamental to both evolution and community function. While biofilms are commonly found in soil-like porous environments, the study of microbial interactions has largely focused on biofilms growing on flat, planar surfaces. Here we use novel microfluidic experiments, mechanistic models, and game theory to study how porous media hydrodynamics can mediate competition between bacterial genotypes. Our experiments reveal a fundamental challenge faced by microbial strains that live in porous environments: cells that rapidly form biofilms tend to block their access to fluid flow and redirect resources to competitors. To understand how these dynamics influence the evolution of bacterial growth rates we couple a model of flow-biofilm interaction with a game theory analysis. This shows that hydrodynamic interactions between competing genotypes give rise to an evolutionarily stable growth rate that stands in stark contrast with that observed in typical laboratory experiments: cells within a biofilm can outcompete other genotypes by growing more slowly. Our work reveals that hydrodynamics can profoundly affect how bacteria compete and evolve in porous environments, the habitat where most bacteria live

Acid pH activation of the PmrA/PmrB two-component regulatory system of Salmonella enterica

Acid pH often triggers changes in gene expression. However, little is known about the identity of the gene products that sense fluctuations in extracytoplasmic pH. The Gram-negative pathogen Salmonella enterica serovar Typhimurium experiences a number of acidic environments both inside and outside animal hosts. Growth in mild acid (pH 5.8) promotes transcription of genes activated by the response regulator PmrA, but the signalling pathway(s) that mediates this response has thus far remained unexplored. Here we report that this activation requires both PmrA's cognate sensor kinase PmrB, which had been previously shown to respond to Fe(3+) and Al(3+), and PmrA's post-translational activator PmrD. Substitution of a conserved histidine or of either one of four conserved glutamic acid residues in the periplasmic domain of PmrB severely decreased or abolished the mild acid-promoted transcription of PmrA-activated genes. The PmrA/PmrB system controls lipopolysaccharide modifications mediating resistance to the antibiotic polymyxin B. Wild-type Salmonella grown at pH 5.8 were > 100 000-fold more resistant to polymyxin B than organisms grown at pH 7.7. Our results suggest that protonation of the PmrB periplasmic histidine and/or of the glutamic acid residues activate the PmrA protein, and that mild acid promotes cellular changes resulting in polymyxin B resistance

Targeted nasal vaccination provides antibody-independent protection against Staphylococcus aureus.

Despite showing promise in preclinical models, anti-Staphylococcus aureus vaccines have failed in clinical trials. To date, approaches have focused on neutralizing/opsonizing antibodies; however, vaccines exclusively inducing cellular immunity have not been studied to formally test whether a cellular-only response can protect against infection. We demonstrate that nasal vaccination with targeted nanoparticles loaded with Staphylococcus aureus antigen protects against acute systemic S. aureus infection in the absence of any antigen-specific antibodies. These findings can help inform future developments in staphylococcal vaccine development and studies into the requirements for protective immunity against S. aureus

On the detectability of the CMSSM light Higgs boson at the Tevatron

We examine the prospects of detecting the light Higgs h^0 of the Constrained MSSM at the Tevatron. To this end we explore the CMSSM parameter space with \mu>0, using a Markov Chain Monte Carlo technique, and apply all relevant collider and cosmological constraints including their uncertainties, as well as those of the Standard Model parameters. Taking 50 GeV < m_{1/2}, m_0 < 4 TeV, |A_0| < 7 TeV and 2 < tan(beta) < 62 as flat priors and using the formalism of Bayesian statistics we find that the 68% posterior probability region for the h^0 mass lies between 115.4 GeV and 120.4 GeV. Otherwise, h^0 is very similar to the Standard Model Higgs boson. Nevertheless, we point out some enhancements in its couplings to bottom and tau pairs, ranging from a few per cent in most of the CMSSM parameter space, up to several per cent in the favored region of tan(beta)\sim 50 and the pseudoscalar Higgs mass of m_A\lsim 1 TeV. We also find that the other Higgs bosons are typically heavier, although not necessarily much heavier. For values of the h^0 mass within the 95% probability range as determined by our analysis, a 95% CL exclusion limit can be set with about 2/fb of integrated luminosity per experiment, or else with 4/fb (12/fb) a 3 sigma evidence (5 sigma discovery) will be guaranteed. We also emphasize that the alternative statistical measure of the mean quality-of-fit favors a somewhat lower Higgs mass range; this implies even more optimistic prospects for the CMSSM light Higgs search than the more conservative Bayesian approach. In conclusion, for the above CMSSM parameter ranges, especially m_0, either some evidence will be found at the Tevatron for the light Higgs boson or, at a high confidence level, the CMSSM will be ruled out.Comment: JHEP versio

What do young athletes implicitly understand about psychological skills?

One reason sport psychologists teach psychological skills is to enhance performance in sport; but the value of psychological skills for young athletes is questionable because of the qualitative and quantitative differences between children and adults in their understanding of abstract concepts such as mental skills. To teach these skills effectively to young athletes, sport psychologists need to appreciate what young athletes implicitly understand about such skills because maturational (e.g., cognitive, social) and environmental (e.g., coaches) factors can influence the progressive development of children and youth. In the present qualitative study, we explored young athletes’ (aged 10–15 years) understanding of four basic psychological skills: goal setting, mental imagery, self-talk, and relaxation. Young athletes (n = 118: 75 males and 43 females) completed an open-ended questionnaire to report their understanding of these four basic psychological skills. Compared with the older youth athletes, the younger youth athletes were less able to explain the meaning of each psychological skill. Goal setting and mental imagery were better understood than self-talk and relaxation. Based on these findings, sport psychologists should consider adapting interventions and psychoeducational programs to match young athletes’ age and developmental level

Cosmological evolution of interacting dark energy in Lorentz violation

The cosmological evolution of an interacting scalar field model in which the scalar field interacts with dark matter, radiation, and baryon via Lorentz violation is investigated. We propose a model of interaction through the effective coupling $\bar{\beta}$. Using dynamical system analysis, we study the linear dynamics of an interacting model and show that the dynamics of critical points are completely controlled by two parameters. Some results can be mentioned as follows. Firstly, the sequence of radiation, the dark matter, and the scalar field dark energy exist and baryons are sub dominant. Secondly, the model also allows the possibility of having a universe in the phantom phase with constant potential. Thirdly, the effective gravitational constant varies with respect to time through $\bar{\beta}$. In particular, we consider a simple case where $\bar{\beta}$ has a quadratic form and has a good agreement with the modified $\Lambda$CDM and quintessence models. Finally, we also calculate the first post--Newtonian parameters for our model.Comment: 14 pages, published versio

Implications for the Constrained MSSM from a new prediction for b to s gamma

We re-examine the properties of the Constrained MSSM in light of updated constraints, paying particular attention to the impact of the recent substantial shift in the Standard Model prediction for BR(B to X_s gamma). With the help of a Markov Chain Monte Carlo scanning technique, we vary all relevant parameters simultaneously and derive Bayesian posterior probability maps. We find that the case of \mu>0 remains favored, and that for \mu<0 it is considerably more difficult to find a good global fit to current constraints. In both cases we find a strong preference for a focus point region. This leads to improved prospects for detecting neutralino dark matter in direct searches, while superpartner searches at the LHC become more problematic, especially when \mu<0. In contrast, prospects for exploring the whole mass range of the lightest Higgs boson at the Tevatron and the LHC remain very good, which should, along with dark matter searches, allow one to gain access to the otherwise experimentally challenging focus point region. An alternative measure of the mean quality-of-fit which we also employ implies that present data are not yet constraining enough to draw more definite conclusions. We also comment on the dependence of our results on the choice of priors and on some other assumptions.Comment: JHEP versio

Observation of isolated high-E_T photons in deep inelastic scattering

First measurements of cross sections for isolated prompt photon production in deep inelastic ep scattering have been made using the ZEUS detector at the HERA electron-proton collider using an integrated luminosity of 121 pb^-1. A signal for isolated photons in the transverse energy and rapidity ranges 5 < E_T^gamma < 10 GeV and -0.7 < eta^gamma < 0.9 was observed for virtualities of the exchanged photon of Q^2 > 35 GeV^2. Cross sections are presented for inclusive prompt photons and for those accompanied by a single jet in the range E_T^jet \geq 6 GeV and -1.5 \leq eta^jet < 1.8. Calculations at order alpha^3alpha_s describe the data reasonably well.Comment: 16 pages, 5 figure

Photoproduction of D∗±D^{*\pm} mesons associated with a leading neutron

The photoproduction of $D^{*\pm} (2010)$ mesons associated with a leading neutron has been observed with the ZEUS detector in $ep$ collisions at HERA using an integrated luminosity of 80 pb$^{-1}$. The neutron carries a large fraction, {$x_L>0.2$}, of the incoming proton beam energy and is detected at very small production angles, {$\theta_n<0.8$ mrad}, an indication of peripheral scattering. The $D^*$ meson is centrally produced with pseudorapidity {$|\eta| 1.9$ GeV}, which is large compared to the average transverse momentum of the neutron of 0.22 GeV. The ratio of neutron-tagged to inclusive $D^*$ production is $8.85\pm 0.93({\rm stat.})^{+0.48}_{-0.61}({\rm syst.})\%$ in the photon-proton center-of-mass energy range {$130 <W<280$ GeV}. The data suggest that the presence of a hard scale enhances the fraction of events with a leading neutron in the final state.Comment: 28 pages, 4 figures, 2 table

Measurement of beauty production in deep inelastic scattering at HERA

The beauty production cross section for deep inelastic scattering events with at least one hard jet in the Breit frame together with a muon has been measured, for photon virtualities Q^2 > 2 GeV^2, with the ZEUS detector at HERA using integrated luminosity of 72 pb^-1. The total visible cross section is sigma_b-bbar (ep -> e jet mu X) = 40.9 +- 5.7 (stat.) +6.0 -4.4 (syst.) pb. The next-to-leading order QCD prediction lies about 2.5 standard deviations below the data. The differential cross sections are in general consistent with the NLO QCD predictions; however at low values of Q^2, Bjorken x, and muon transverse momentum, and high values of jet transverse energy and muon pseudorapidity, the prediction is about two standard deviations below the data.Comment: 18 pages, 4 figure