Proinflammatory cytokine levels in hyperlipidemic patients with periodontitis after periodontal treatment

OBJECTIVE: The aim of this study was to evaluate the effects of periodontal treatment on serum and gingival crevicular fluid (GCF) proinflammatory cytokine levels in hyperlipidemic patients with periodontitis. MATERIALS AND METHODS: Fifty-two patients with hyperlipidemia and periodontitis and 28 systemically healthy controls with periodontitis (C) were included in the study. Hyperlipidemic groups were divided into two groups as suggested diet (HD) and prescribed statin (HS). The clinical periodontal parameters, fasting venous blood, and GCF samples were obtained, and serum tumor necrosis factor-alpha (TNF-a), interleukin (IL) 1-beta, and IL-6 levels were evaluated at baseline and at 3 months follow-up (3MFU) after the completion of the non-surgical periodontal treatment that included scaling and root planning. RESULTS: Percentage of bleeding on probing was significantly higher in the HS group than both the HD and C groups. In the HD and HS groups, there were significant decreases in serum IL-6 andGCFTNF-a levels between the 3MFUand baseline. Asignificant decrease was also found in GCF IL-6 at the end of the study period in the HS group. CONCLUSION: The combination of the periodontal therapy and antilipemic treatment may provide beneficial effects on the metabolic and inflammatory control of hyperlipidemia. Oral Diseases (2012) 18, 299-30

PERİODONTİTİSLİ HİPERLİPİDEMİLİ BİREYLERDE PERİODONTAL TEDAVİNİN SALYA LAKTOFERRİN SEVİYELERİ ÜZERİNE ETKİSİNİN DEĞERLENDİRİLMESİ [The Effect of Periodontal Treatment on Salivary Lactoferrin Levels in Hyperlipidemic Patients with

ÖZET Amaç: Bu çalışmada amaç periodontitisli hiperlipidemili bireylerde cerrahi olmayan periodontal tedavinin salya laktoferrin (sLF), seviyeleri üzerine etkisinin değerlendirilmesidir. Gereç ve Yöntem: Bu çalışmada yaşları 30 ila 57 arasında değişen 52 hiperlipidemili ve yaşları 31 ila 54 arasında değişen 28 sistemik olarak sağlıklı birey (K) olmak üzere toplam 80 periodontitisli birey yer aldı. Hiperlipidemik bireyler diyet önerilen (HD) ve statin önerilen (HS) bireyler olmak üzere iki gruba ayrıldı. Tüm bireylerin plak indeksi (Pİ), gingival indeks (Gİ), cep derinliği (CD), klinik ataçman seviyesi (KAS) ve sondlamada kanama varlığı yüzdesi (SK (%))’ ni içeren ölçümlerden oluşan klinik periodontal parametreleri kaydedildi. Serum lipidleri ve sLF seviyeleri değerlendirildi. Bütün hastalara cerrahi olmayan periodontal tedavi uygulanarak, periodontal tedavinin tamamlanmasını takiben 3. ayda klinik periodontal parametreler, sLF ve serum lipidleri tekrar değerlendirildi. Bulgular: HS grubu, HD ve K gruplarına göre artmış başlangıç SK (%) değerine sahipti. HD grubunda başlangıç sLF ile CD, KAS ve SK (%), HS grubunda başlangıç sLF ile Pİ, SK (%) ve KAS arasında önemli ilişki saptandı. Hiperlipidemili gruplarda, SK (%) ve total kolesterol/yüksek densiteli lipoprotein değerleri ile sLF seviyesi arasındaki pozitif korelasyonlar başlangıçta anlamlı iken (p 0.05), 3. ayda sLF ile klinik periodontal ve serum lipid parametreleri arasında önemli korelasyon gözlenmedi. Sonuç: Periodontal tedavi hiperlipidemili bireylerde periodontal inflamasyonun azaltılması ile ilişkili olarak sLF seviyelerinde ve aterojenik lipid profilinde azalmalara neden olmuştur. Antimikrobiyal ve lipid metabolizmasını düzenleyici bir enzim olarak LF’nin periodontal hastalık ve hiperlipidemi ilişkisindeki rolünü değerlendirmeye yönelik olarak daha geniş popülasyonlarda ileri çalışmalara ihtiyaç vardır. Anahtar kelimeler: Periodontitis, Hiperlipidemi, Salya Laktoferrin ABSTRACT Objective: The purpose of this study was to evaluate the effect of non-surgical periodontal treatment on salivary lactoferrin (sLF) levels in hyperlipidemic patients with periodontitis. Material and Method: There were 52 patients with hyperlipidemia aged 30-57 and 28 systemically healthy controls (C) aged 31-54, totally 80 subjects with periodontitis in this study. Hyperlipidemic subjects were divided into two groups as suggested diet (HD) and prescribed statin (HS). The periodontal parameters, including plaque index (PI), gingival index (GI), probing pocket (PPD) depth, percentage of bleeding on probing (BOP (%)), and clinical attachment level (CAL) were recorded. Serum lipids and sLF levels were evaluated. Clinical periodontal parameters, sLF and serum lipids were reevaluated in all subjects at three months after the completion of the non-surgical periodontal treatment. Results: HS group had a higher value of baseline BOP (%) when compared to C and HD groups. Whereas in HD group there were significant correlations among baseline sLF, CD, CAL and BOP (%), in HS group there were significant correlations among baseline sLF, PI, BOP (%) and CAL. In hyperlipidemic groups, positive correlations between sLF, BOP (%) and total cholesterol/high density lipoprotein at baseline (p 0.05), while at the third month there were not significant correlations between sLF, clinical periodontal and serum lipid parameters. Conclusion: The periodontal treatment led to decreases in sLF and atherogenic lipid profile in hyperlipidemic patients. Further longitudinal studies in larger populations are needed to clarify the role of the LF in the association between periodontal disease and hyperlipidemia. Key words: Periodontitis; Hyperlipidemia; Salivary Lactoferri

Evaluation of Lipid Peroxidation and Oxidative DNA Damage in Patients With Periodontitis and Hyperlipidemia

Background: The purpose of this study is to determine the serum levels of malondialdehyde (MDA), as a lipid peroxidation marker, and 8-hydroxydeoxyguanosine (8-OHdG), as an oxidative DNA damage marker, in patients with chronic periodontitis (CP) and hyperlipidemia

Serum Lp-PLA2: as a novel viewpoint in periodontal treatment of hyperlipidaemics

Background/aim: To evaluate the effects of periodontal treatment on serum lipoprotein-associated phospholipase A2 (Lp-PLA2) and C-reactive protein (CRP) levels in hyperlipidaemic patients with periodontitis

Gingival crevicular fluid tissue/blood vessel-type plasminogen activator and plasminogen activator inhibitor-2 levels in patients with rheumatoid arthritis: Effects of nonsurgical periodontal therapy

WOS: 000399955500030PubMed ID: 27781272Background and ObjectiveThe aim of this study was to evaluate the effect of nonsurgical periodontal therapy on clinical parameters and gingival crevicular fluid levels of tissue/blood vessel-type plasminogen activator (t-PA) and plasminogen activator inhibitor-2 (PAI-2) in patients with periodontitis, with or without rheumatoid arthritis (RA). Material and MethodsFifteen patients with RA and chronic periodontitis (RA-P), 15 systemically healthy patients with chronic periodontitis (H-P) and 15 periodontally and systemically healthy volunteers (C) were included in the study. Plaque index, gingival index, probing pocket depth, clinical attachment level, bleeding on probing, gingival crevicular fluid t-PA and PAI-2 levels, erythrocyte sedimentation rate, serum C-reactive protein and disease activity score were evaluated at baseline and 3 mo after mechanical nonsurgical periodontal therapy. ResultsAll periodontal clinical parameters were significantly higher in the RA-P and H-P groups compared with the C group (p < 0.001) and decreased significantly after treatment (p < 0.001). Pretreatment t-PA levels were highest in the RA-P group and significantly decreased post-treatment (p = 0.047). Pre- and post-treatment PAI-2 levels were significantly lower in controls compared with both periodontitis groups (p < 0.05). Gingival crevicular fluid volume and the levels of t-PA and PAI-2 were significantly correlated. ConclusionIn patients with periodontitis and RA, nonsurgical periodontal therapy reduced the pretreatment gingival crevicular fluid t-PA levels, which were significantly correlated with gingival crevicular fluid PAI-2 levels. The significantly higher t-PA and PAI-2 gingival crevicular fluid levels in periodontal patients, regardless of systemic status, suggest that the plasminogen activating system plays a role in the disease process of periodontitis.Ankara University Scientific Research Projects Office, Ankara, Turkey [08B3334005]This study was supported by the Ankara University Scientific Research Projects Office, Ankara, Turkey (08B3334005). The authors declare that they have no conflicts of interest

Antioxidant effects of melatonin in heart tissue after induction of experimental periodontitis in rats

The aim of this study was to evaluate the effects of melatonin on the oxidative stress in heart tissues after induction of experimental periodontitis in rats. Thirty Wistar Albino male rats were divided into four groups as follows: healthy + saline solution (Hs, n = 7), healthy + melatonin (Hm, n = 7), periodontitis + saline solution (Ps, n = 8), and periodontitis + melatonin (Pm, n = 8). Experimental periodontitis was induced using a ligature placed at the gingival margin of the maxillary second molars. Melatonin was applied intraperitoneally (10 mg/kg) every day for 2 weeks. After sacrificing the rats, serum levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) levels, and melatonin levels were evaluated. The Pm group exhibited lower alveolar bone loss than the Ps group. Melatonin levels increased in the periodontitis groups, and the Pm group had lower MDA levels and higher GSH-Px levels than the Ps group. These findings suggest that melatonin administration reduces MDA and increases GSH-Px levels in heart tissue, and these effects may be due to its antioxidant properties. Further studies are needed to understand the effects of melatonin on the association between periodontitis and cardiovascular disease