Romanesque and territory. The construction materials of Sardinian medieval churches: new approaches to the valorization, conservation and restoration

This paper is intended to illustrate a multidisciplinary research project devoted to the study of the constructive materials of the Romanesque churches in Sardinia during the “Giudicati” period (11th -13th centuries). The project focuses on the relationship between a selection of monuments and their territory, both from a historical-architectural perspective and from a more modern perspective addressing future restoration works. The methodologies of the traditional art-historical research (study of bibliographic, epigraphic and archival sources, formal reading of artifacts) are flanked by new technologies: digital surveys executed with a 3D laser-scanner, analyses of the materials (stones, mortars, bricks) with different instrumental methods: X-ray fluorescence (XRF) and inductively coupled mass spectrometry (ICP-MS) for chemical composition, X-ray diffractometer (XRD) to determine the alteration phases (e.g., soluble salts), optical microscopy and electronic (SEM) to study textures, mineral assemblages and microstructures, termogravimetric/differential scanning, calorimetric analysis (TG/DTA) for the composition of the binder mortars. This multidisciplinary approach allows the achieving of important results in an archaeometric context: 1) from a historical point of view, with the possible identification of ancient traffics, trade routes, sources of raw materials, construction phases, wall textures; 2) from a conservative point of view, by studying chemical and physical weathering processes of stone materials compatible for replacement in case of future restoration works. Sardinian Romanesque architectural heritage is particularly remarkable: about 200 churches of different types and sizes, with the almost exclusive use of cut stones. Bi- or poly-chromy, deriving from the use of different building materials, characterizes many of these monuments, becoming also a vehicle for political and cultural meanings. The paper will present some case studies aimed to illustrate the progress of the project and the results achieved

Graffiti removal from granite cladding by abrasive water-jet

This study focuses on the assessment of a graffiti removal system based on abrasive water-jet technology (AWJ). Spray paints were applied on samples of an important Italian granite commercially-named as “Rosa Limbara”. This granite has been used worldwide as dimension stone for paving and cladding. Two different commercial abrasives have been added in the high-pressure water-jet in order to clean the granite saw-plane surfaces from graffiti. After a check by the naked eye, different criteria have been chosen for assessing the effectiveness of the cleaning process: the similarity in roughness (Ra, Rz, and Rmax) between the original surface and the one resulted after cleaning, the total colour difference (ΔE) and the depth of material removed by the AWJ action. The tests showed that excellent cleaning results can be achieved with both abrasives by correctly combining the operational parameters of the technology (i.e., water pressure, spacing between passes, stand-off distance, travel speed and abrasive flow-rate) without giving any damage to the stone material. The two abrasives showed good results in total colour difference although one of them gave better results in both roughness difference and material removal. Economic considerations on paint removal with AWJ technology are also offered

Voltage- and Temperature-Dependent Allosteric Modulation of α7 Nicotinic Receptors by PNU120596

This is the final version of the article. Available from Frontiers Media via the DOI in this record.Alpha7 nicotinic acetylcholine receptors (α7 nAChR) are widely distributed throughout the central nervous system and are found at particularly high levels in the hippocampus and cortex. Several lines of evidence indicate that pharmacological enhancement of α7 nAChRs function could be a potential therapeutic route to alleviate disease-related cognitive deficits. A recent pharmacological approach adopted to increase α7 nAChR activity has been to identify selective positive allosteric modulators (PAMs). α7 nAChR PAMs have been divided into two classes: type I PAMs increase agonist potency with only subtle effects on kinetics, whereas type II agents produce additional dramatic effects on desensitization and deactivation kinetics. Here we report novel observations concerning the pharmacology of the canonical type II PAM, PNU120596. Using patch clamp analysis of acetylcholine (ACh)-mediated currents through recombinant rat α7 nAChR we show that positive allosteric modulation measured in two different ways is greatly attenuated when the temperature is raised to near physiological levels. Furthermore, PNU120596 largely removes the strong inward rectification usually exhibited by α7 nAChR-mediated responses

Contrasting multi-taxa diversity patterns between abandoned and non-intensively managed forests in the southern Dolomites

The abandonment of silvicultural activities can lead to changes in species richness and composition of biological communities, when compared to those found in managed forests. The aim of this study was to compare the multi-taxonomical diversity of two mature silver fir-beech-spruce forests in the southern Dolomites (Italy), corresponding to the European Union habitat type 9130. The two sites share similar ecological and structural characteristics, but differ in their recent management histories. In the last 50 years, one site underwent non-intensive management, while the other was left unmanaged and was included in a forest reserve. The species richness and composition of eight taxa were surveyed in the two sites between 2009 and 2011. The difference in mean species richness between the two forest management types was tested through permutation tests, while differences in species composition were tested by principal coordinates analysis and the permutational multivariate analysis of variance. Mean species richness of soil macrofungi, deadwood lichens, bark beetles, and longhorn beetles were significantly higher in the abandoned than in the non-intensively managed forests. Deadwood fungi and epiphytic lichens did not differ in mean species richness between the two study sites, while mean species richness of ground beetles and birds were higher in the non-intensively managed than in the abandoned forest. Significant differences in species composition between the two sites were found for all the taxa, except for longhorn beetles. These results indicate that improving forest landscape heterogeneity through the creation of a mosaic of abandoned and extensively managed forests should better fulfill the requirements of ecologically different taxa

Pyroclastic Stones as Building Materials in Medieval Romanesque Architecture of Sardinia (Italy): Chemical-Physical Features of Rocks and Associated Alterations

The paper discusses the chemical-physical-petrographic features and decay processes of San Nicola Church (11th−14th century) building materials, one of the most representative Medieval Churches in Sardinia, stylistically attributable to the Romanesque architecture. The monument was built up into two stages and shows a characteristic size uniformity of the ashlars. The masonry is mainly made up of rhyodacitic pyroclastites belonging to the Sardinian Eocene-Miocene magmatic phase (38–15 My). These volcanic rocks were widely used in Medieval architecture for the excellent workability, but its minero-petrographic features greatly favor the decay, due to the action of chemical-physical alteration processes. Although the alteration degree and macroscopic forms of decay vary from zone to zone in the monument, according to the different compositional features of the volcanic stones, weathering and exposure condition, the pyroclastic rocks are generally affected by greater alteration than other igneous lithotypes, due to their petrographic-volcanological characteristics (e.g., low-medium welding grade, medium-high porosity). The results concerning mineralogical and petrographic features, respectively, carried out by the application of X-Ray Powder Diffraction (XRPD), Optical Microscope (OM), and Scanning Electron Microscope (SEM) techniques, both on fresh building rocks and associated alterations, are here presented and discussed. Furthermore, the relationships between fresh pyroclastites and alteration processes affecting these rocks will be discussed

Boschi vetusti e riserve forestali nel Veneto: patrimoni di biodiversita\u300

Vengono sintetizzate le criticita\u300 emergenti legate alle dinamiche di cambiamento della biodiversita\u300 nelle foreste del Veneto anche esaminando i dati delle politiche forestali degli ultimi decenni e delle trasformazioni di boschi e foreste di montagna, collina e pianura conseguenti all\u2019abbandono colturale e ad iniziative locali di contrasto alle alterazioni del paesaggio forestale, di miglioramento ambientale ed estetico e di difesa della biodiversita\u300 forestale e naturalistica dei territori del Veneto. Seppur in un confronto europeo l'Italia e\u300 forse uno degli stati piu\u300 virtuosi nella gestione del patrimonio boschivo, sia in termini di risparmio di biomassa (35% di prelievo sull'incremento contro la media europea di oltre 60%) che per modalita\u300 di prelievo (e\u300 uno dei pochi stati dove il taglio a raso e\u300 vietato e, a differenza di molti paesi europei, non ammette l'imboschimento con specie esotiche), in questo approfondimento vengono esaminati gli scenari possibili di riferimento per una programmazione piu\u300 mirata, incisiva ed innovativa delle politiche di tutela della biodiversita\u300 forestale del Veneto, guardando ad alcune esperienze europee volte alla tutela e valorizzazione dei boschi antichi o vetusti. Boschi e foreste si stanno espandendo nel Veneto anche in pianura: ma questo trend e\u300 frutto dell\u2019abbandono colturale e/o conseguenza di una programmazione territoriale adeguata? Si propongono linee guida per iniziative locali di contrasto alla banalizzazione estetica ed ambientale del paesaggio e per contro di miglioramento della difesa della biodiversita\u300 forestale e naturalistica dei territori del Veneto partendo dalla tutela del patrimonio genetico dei \u201cboschi vetusti\u201d

What do measures of patient satisfaction with the doctor tell us?

Objective: To gain an understanding of how patient satisfaction (PS) with the doctor (PSD) is conceptualized through an empirical review of how it is currently being measured. The content of PS questionnaire items was examined to (a) determine the primary domains underlying PSD, and (b) summarize the specific doctor-related characteristics and behaviors, and patient-related perceptions, composing each domain. Methods: A scoping review of empirical articles that assessed PSD published from 2000 to November 2013. MEDLINE and PsycINFO databases were searched. Results: The literature search yielded 1726 articles, 316 of which fulfilled study inclusion criteria. PSD was realized in one of four health contexts, with questions being embedded in a larger questionnaire that assessed PS with either: (1) overall healthcare, (2) a specific medical encounter, or (3) the healthcare team. In the fourth context, PSD was the questionnaire's sole focus. Five broad domains underlying PSD were revealed: (1) Communication Attributes; (2) Relational Conduct; (3) Technical Skill/Knowledge; (4) Personal Qualities; and (5) Availability/Accessibility. Conclusions: Careful consideration of measurement goals and purposes is necessary when selecting a PSD measure. Practice implications: The five emergent domains underlying PSD point to potential key areas of physician training and foci for quality assessment

On reminder effects, drop-outs and dominance: evidence from an online experiment on charitable giving

We present the results of an experiment that (a) shows the usefulness of screening out drop-outs and (b) tests whether different methods of payment and reminder intervals affect charitable giving. Following a lab session, participants could make online donations to charity for a total duration of three months. Our procedure justifying the exclusion of drop-outs consists in requiring participants to collect payments in person flexibly and as known in advance and as highlighted to them later. Our interpretation is that participants who failed to collect their positive payments under these circumstances are likely not to satisfy dominance. If we restrict the sample to subjects who did not drop out, but not otherwise, reminders significantly increase the overall amount of charitable giving. We also find that weekly reminders are no more effective than monthly reminders in increasing charitable giving, and that, in our three months duration experiment, standing orders do not increase giving relative to one-off donations

Improvement of endurance of DMD animal model using natural polyphenols

Duchenne Muscular Dystrophy (DMD), the most common form of muscular dystrophy, is characterized by muscular wasting caused by dystrophin deficiency that ultimately ends in force reduction and premature death. In addition to primary genetic defect, several mechanisms contribute to DMD pathogenesis. Recently, antioxidant supplementation was shown to be effective in the treatment of multiple diseases including muscular dystrophy. Different mechanisms were hypothesized such as reduced hydroxyl radicals, nuclear factor-\u3baB deactivation and NO protection from inactivation. Following these promising evidences, we investigated the effect of the administration of a mix of dietary natural polyphenols (ProAbe) on dystrophic mdx mice in term of muscular architecture and functionality. We observed a reduction of muscle fibrosis deposition and myofiber necrosis together with an amelioration of vascolarization. More importantly, the recovery of the morphological features of dystrophic muscle leads to an improvement of the endurance of treated dystrophic mice. Our data confirmed that ProAbe-based diet may represent a strategy to co-adjuvate the treatment of DMD

Inositol 1,4,5-trisphosphate (IP3)-dependent Ca2+ signaling mediates delayed myogenesis in Duchenne muscular dystrophy fetal muscle

Duchenne muscular dystrophy (DMD) is a progressive neuromuscular disorder characterized by muscle wasting and premature death. The defective gene is dystrophin, a structural protein, absence of which causes membrane fragility and myofiber necrosis. Several lines of evidence showed that in adult DMD patients dystrophin is involved in signaling pathways that regulate calcium homeostasis and differentiation programs. However, secondary aspects of the disease, such as inflammation and fibrosis development, might represent a bias in the analysis. Because fetal muscle is not influenced by gravity and does not suffer from mechanical load and/or inflammation, we investigated 12-week-old fetal DMD skeletal muscles, highlighting for the first time early alterations in signaling pathways mediated by the absence of dystrophin itself. We found that PLC/IP3/IP3R/Ryr1/Ca2+ signaling is widely active in fetal DMD skeletal muscles and, through the calcium-dependent PKCa protein, exerts a fundamental regulatory role in delaying myogenesis and in myofiber commitment. These data provide new insights into the origin of DMD pathology during muscle development