Characterization of Multiple-Herbicide-Resistant Echinochloa colona from Arkansas

Echinochloa species are highly adaptive weeds that have the potential to impact crops in a variety of environments. This has positioned them as the most problematic weeds in a number of USA cropping systems with some species having the distinction of the \u27worst herbicide-resistant weeds\u27 in the world. Recent evidence has positioned Echinochloa colona (junglerice) as the most dominant in Arkansas and throughout the Mid-South, USA, especially in rice (Oryza sativa L.) and soybean (Glycine max L.) production fields. A history of extensive herbicide-use for management and a lack of integrated or diverse approaches to management have led to rampant herbicide resistance within production fields. The goal of this research is to assess herbicide-resistant E. colona from the field to the genomic level. Five objectives are the focus of this research: (1) characterize the current status of herbicide-resistant Echinochloa in Arkansas rice and assess the distribution of resistance patterns with time, (2) evaluate the underlying mechanisms driving multiple resistance in E. colona (3) assemble a de novo transcriptome of E. colona and assess the mechanisms of resistance to quinclorac, (4) use the transcriptome to characterize the response to propanil in multiple-resistant and susceptible E. colona and identify the basis for resistance to propanil, and (5) use the transcriptome analysis in response to multiple herbicides to identify the biological functions of susceptible and resistant E. colona following herbicide treatment. This research used a population that is highly resistant to propanil and quinclorac, and with elevated tolerance to cyhalofop and glufosinate. This E. colona accession has non-target site resistance via independent mechanisms involving cytochrome P450 enzymes and glycosyltransferase enzymes for propanil and quinclorac, respectively. Herbicide resistance co-evolved with abiotic stress tolerance potentially through the enhancement of the trehalose biosynthetic pathway. This research had generated the first assembled transcriptome of E. colona and description of the transcriptomic responses to the common rice herbicides cyhalofop, propanil, and quinclorac, as well as the non-selective herbicide glufosinate. This research generated the first global transcriptome comparison across multiple herbicides, characterizing the patterns of gene expression following herbicide treatment with diverse herbicide modes of action

Assessing location attractiveness for manufacturing automobiles

Purpose: Evaluating country manufacturing location attractiveness on various performance measures deepens the analysis and provides a more informed basis for manufacturing site selection versus reliance on labor rates alone. A short list of countries can be used to drive regional considerations for site-specific selection within a country. Design/methodology/approach: The two-step multi attribute decision model contains an initial filter layer to require minimum values for low weighted attributes and provides a rank order utility score for twenty three countries studied. The model contains 11 key explanatory variables with Labor Rate, Material Cost, and Logistics making up the top 3 attributes and representing 54% percent of the model weights. Findings: We propose a multi attribute decision framework for strategically assessing the attractiveness of a country as a location for manufacturing automobiles. Research limitations/implications: Consideration of country level wage variation, specific tariffs, and other economic incentives provides a secondary analysis after the initial list of candidate countries is defined. Practical implications: The results of our modeling shows China, India, and Mexico are currently the top ranked countries for manufacturing attractiveness. These three markets hold the highest utility scores throughout sensitivity analysis on the labor rate attribute weight rating, highlighting the strength and potential of manufacturing in China, India, and Mexico. Originality/value: Combining MAUT with regression analysis to simplify model to core factors then using a “must have” layer to handle extreme impacts of low weight factors and allowing for ease of repeatabilityPeer Reviewe

Karyomegalic interstitial nephritis and DNA damage-induced polyploidy in fan1 nuclease-defective knock-in mice

The Fan1 endonuclease is required for repair of DNA interstrand cross-links (ICLs). Mutations in human Fan1 cause karyomegalic interstitial nephritis (KIN), but it is unclear whether defective ICL repair is responsible or whether Fan1 nuclease activity is relevant. We show that Fan1 nuclease-defective (Fan1nd/nd) mice develop a mild form of KIN. The karyomegalic nuclei from Fan1nd/nd kidneys are polyploid, and fibroblasts from Fan1nd/nd mice become polyploid upon ICL induction, suggesting that defective ICL repair causes karyomegaly. Thus, Fan1 nuclease activity promotes ICL repair in a manner that controls ploidy, a role that we show is not shared by the Fanconi anemia pathway or the Slx4–Slx1 nuclease also involved in ICL repair

How pressure affects costs of power conversion machinery in compressed air energy storage; part II: Heat exchangers

In the field of compressed air energy storage, a critical economic aspect that has been overlooked in existing literature relates to the influence of storage pressure on the capital cost of power conversion system. In Part I, a comprehensive study was conducted to address this question focusing on compressors and expanders. This part is devoted to the heat exchangers and basically assesses the engineering rationale behind the relationship between the cost per kW for HXs and operating pressure. Based on the performed analysis, the operating pressure of a HX impacts two crucial cost-related factors: the heat transfer area and required tube thicknesses. Higher operating pressures are associated with the smaller heat transfer area tending to lower costs, but increasing pressure raises tube thickness requirements, tending to increase costs. Below approximately 200 bar, the former effect prevails over the latter, leading to cost reductions with rising pressure. Conversely, at higher pressures, the latter effect outweighs the former, resulting in cost increases with increasing pressure. On the other hand, as the number of compression stages is increased to attain higher storage pressures, there is a noteworthy variation in the cost contribution of HXs. Specifically, the contribution of HX costs within the PCS machinery escalates from 10% at a storage pressure of 30 bar to approximately 35% at a storage pressure of 350 bar. This cost increase is accompanied by a substantial reduction in costs associated with other PCS machinery components (compressors and expanders), ultimately justifying the advantages of operating at higher storage pressures

How pressure affects costs of power conversion machinery in compressed air energy storage; Part I: Compressors and expanders

This study addresses a critical economic aspect in compressed air energy storage that has not been discussed much in existing literature: the impact of operating pressure on machinery capital cots. It aims to answer whether the cost per unit of power for power conversion systems changes with the maximum storage pressure. Considering that higher storage pressures are associated with greater energy density, enhanced energy storage capabilities and improved system efficiency. This paper helps clarify uncertainties in initial cost estimations for power-generation plants. Effects of operating pressure on the components and overall sizes and consequently costs of power conversion machinery are individually investigated in two parts. Part I encompasses the compressor and expanders, and part II comprehensively discusses the effects of the operating pressure on the costs of heat exchangers. The analysis employs a conceptual engineering approach, revealing that higher intake pressure reduces overall compressor/expander size, leading to cost savings. Additionally, increasing the number of compression stages for higher storage pressures enhances exergy storage cost-effectiveness. To establish an advanced adiabatic CAES plant with a storage pressure of 200 bar instead of 50 bar, there is potential for a 6 % reduction in $/kW expenditure

Adiabatic Compressed Air Energy Storage system performance with application-oriented designed axial-flow compressor

Medium and long-duration energy storage systems are expected to play a critical role in the transition towards electrical grids powered by renewable energy sources. ACAES is a promising solution, capable of handling power and energy ratings over hundreds of MW and MWh, respectively. One challenge with ACAES is achieving the required highly efficient operation in the compressor over the range of conditions encountered in the system as the pressure in the air store changes. In this paper, an application-oriented axial-flow compressor is designed, aiming towards efficient operation throughout the operation range, whilst also associating the performance prediction to a practical compressor geometry. A two-step design methodology based on inviscid, axisymmetric flow conditions has been implemented, leading to the flowtrack, blade-row geometries and the compressor performance map. The compressor model is integrated into an ACAES model, including two compression spools, two expansion stages with preheat, a constant volume high pressure storage operating between 5.5 and 7.7 MPa and two separate Thermal Energy Storage units. While the existing ACAES literature either ignores the transient off-design operation or uses generic numerical correlations (which are not associated to a particular geometry), the key novelty of this paper is the application of a detailed design method for turbomachinery to ACAES. The results indicate that the designed compressor requires 33 stages over the two spools, and is able to operate efficiently over the storage pressure range, showing that if the application-oriented design procedure is applied to the compressor, it does not stop ACAES reaching 70% round-trip efficiency, outputting 35MW for approximately 15 h. Importantly, the specific ACAES requirement of conserving heat at higher temperatures has been fulfilled by decreasing the number of intercoolers. Finally, it is recommended that a similar level of scrutiny is applied to the other components (i.e. expanders, heat exchangers and TES units), keeping in mind the unique set of operational requirements of ACAES. This work is an important step towards removing the common misconception that off-the-shelf components can be easily be used in typical ACAES designs

Pregnancy-Associated Hypertension in Glucose-Intolerant Pregnancy and Subsequent Metabolic Syndrome

To evaluate whether pregnancy-associated hypertension (preeclampsia or gestational hypertension), among women with varying degrees of glucose intolerance during pregnancy is associated with maternal metabolic syndrome 5-10 years later

High [CO2] and Temperature Increase Resistance to Cyhalofop-Butyl in Multiple-Resistant Echinochloa colona

Changes in the environment, specifically rising temperature and increasing atmospheric carbon dioxide concentration [CO2], can alter the growth and physiology of weedy plants. These changes could alter herbicide efficacy, crop-weed interaction, and weed management. The objectives of this research were to quantify the effects of increased atmospheric [CO2] and temperature on absorption, translocation and efficacy of cyhalofop-butyl on multiple-resistant (MR) and susceptible (S) Echinochloa colona genotypes. E. colona, or junglerice, is a troublesome weed in rice and in agronomic and horticultural crops worldwide. Cyhalofop-butyl is a grass herbicide that selectively controls Echinochloa spp. in rice. Maximum 14C-cyhalofop-butyl absorption occurred at 120 h after herbicide treatment (HAT) with >97% of cyhalofop-butyl retained in the treated leaf regardless of [CO2], temperature, or genotype. Neither temperature nor [CO2] affected herbicide absorption into the leaf. The translocation of herbicide was slightly reduced in the MR plants vs. S plants either under elevated [CO2] or high temperature. Although plants grown under high [CO2] or high temperature were taller than those in ambient conditions, neither high [CO2] nor high temperature reduced the herbicide efficacy on susceptible plants. However, herbicide efficacy was reduced on MR plants grown under high [CO2] or high temperature about 50% compared to MR plants at ambient conditions. High [CO2] and high temperature increased the resistance level of MR E. colona to cyhalofop-butyl. To mitigate rapid resistance evolution under a changing climate, weed management practitioners must implement measures to reduce the herbicide selection pressure. These measures include reduction of weed population size through reduction of the soil seedbank, ensuring complete control of current infestations with multiple herbicide modes of action in mixture and in sequence, augmenting herbicides with mechanical control where possible, rotation with weed-competitive crops, use of weed-competitive cultivars, use of weed-suppressive cover crops, and other practices recommended for integrated weed management

Antimicrobial resistance among migrants in Europe: a systematic review and meta-analysis

BACKGROUND: Rates of antimicrobial resistance (AMR) are rising globally and there is concern that increased migration is contributing to the burden of antibiotic resistance in Europe. However, the effect of migration on the burden of AMR in Europe has not yet been comprehensively examined. Therefore, we did a systematic review and meta-analysis to identify and synthesise data for AMR carriage or infection in migrants to Europe to examine differences in patterns of AMR across migrant groups and in different settings. METHODS: For this systematic review and meta-analysis, we searched MEDLINE, Embase, PubMed, and Scopus with no language restrictions from Jan 1, 2000, to Jan 18, 2017, for primary data from observational studies reporting antibacterial resistance in common bacterial pathogens among migrants to 21 European Union-15 and European Economic Area countries. To be eligible for inclusion, studies had to report data on carriage or infection with laboratory-confirmed antibiotic-resistant organisms in migrant populations. We extracted data from eligible studies and assessed quality using piloted, standardised forms. We did not examine drug resistance in tuberculosis and excluded articles solely reporting on this parameter. We also excluded articles in which migrant status was determined by ethnicity, country of birth of participants' parents, or was not defined, and articles in which data were not disaggregated by migrant status. Outcomes were carriage of or infection with antibiotic-resistant organisms. We used random-effects models to calculate the pooled prevalence of each outcome. The study protocol is registered with PROSPERO, number CRD42016043681. FINDINGS: We identified 2274 articles, of which 23 observational studies reporting on antibiotic resistance in 2319 migrants were included. The pooled prevalence of any AMR carriage or AMR infection in migrants was 25·4% (95% CI 19·1-31·8; I2 =98%), including meticillin-resistant Staphylococcus aureus (7·8%, 4·8-10·7; I2 =92%) and antibiotic-resistant Gram-negative bacteria (27·2%, 17·6-36·8; I2 =94%). The pooled prevalence of any AMR carriage or infection was higher in refugees and asylum seekers (33·0%, 18·3-47·6; I2 =98%) than in other migrant groups (6·6%, 1·8-11·3; I2 =92%). The pooled prevalence of antibiotic-resistant organisms was slightly higher in high-migrant community settings (33·1%, 11·1-55·1; I2 =96%) than in migrants in hospitals (24·3%, 16·1-32·6; I2 =98%). We did not find evidence of high rates of transmission of AMR from migrant to host populations. INTERPRETATION: Migrants are exposed to conditions favouring the emergence of drug resistance during transit and in host countries in Europe. Increased antibiotic resistance among refugees and asylum seekers and in high-migrant community settings (such as refugee camps and detention facilities) highlights the need for improved living conditions, access to health care, and initiatives to facilitate detection of and appropriate high-quality treatment for antibiotic-resistant infections during transit and in host countries. Protocols for the prevention and control of infection and for antibiotic surveillance need to be integrated in all aspects of health care, which should be accessible for all migrant groups, and should target determinants of AMR before, during, and after migration. FUNDING: UK National Institute for Health Research Imperial Biomedical Research Centre, Imperial College Healthcare Charity, the Wellcome Trust, and UK National Institute for Health Research Health Protection Research Unit in Healthcare-associated Infections and Antimictobial Resistance at Imperial College London