DNA Sampling: a method for probing protein binding at specific loci on bacterial chromosomes

We describe a protocol, DNA sampling, for the rapid isolation of specific segments of DNA, together with bound proteins, from Escherichia coli K-12. The DNA to be sampled is generated as a discrete fragment within cells by the yeast I-SceI meganuclease, and is purified using FLAG-tagged LacI repressor and beads carrying anti-FLAG antibody. We illustrate the method by investigating the proteins bound to the colicin K gene regulatory region, either before or after induction of the colicin K gene promoter

Action des aromates sur l'appétence des Vaches laitières. Méthode expérimentale et test statistique

Craplet Camille, Dejardin J. Action des aromates sur l’appétence des vaches laitières. Méthode expérimentale et test statistique. In: Bulletin de l'Académie Vétérinaire de France tome 121 n°8, 1968. pp. 343-359

"Live” (stained) benthic foraminiferal living depths, stable isotopes, and taxonomy offshore South Georgia, Southern Ocean: implications for calcification depths

It is widely held that benthic foraminifera exhibit species-specific calcification depth preferences, with their tests recording sediment pore water chemistry at that depth (i.e. stable isotope and trace metal compositions). This assumed depth habitat-specific pore water chemistry relationship has been used to reconstruct various palaeoenvironmental parameters, such as bottom water oxygenation. However, many deep-water foraminiferal studies show wide intra-species variation in sediment living depth but relatively narrow intra-species variation in stable isotope composition. To investigate this depth habitat- stable isotope relationship on the shelf we analysed depth distribution and stable isotopes of “living” (Rose Bengal stained) benthic foraminifera from two box cores collected on the South Georgia shelf (ranging from 250–300 m water depth). We provide a comprehensive taxonomic analysis of the benthic fauna, comprising 79 taxonomic groupings. The fauna shows close affinities with shelf assemblages from around Antarctica. We find “live” specimens of a number calcareous species from a range of depths in the sediment column. Stable isotope ratios (δ13C and δ18O) were measured on stained specimens of three species, Astrononion echolsi, Cassidulinoides porrectus and Buccella sp. 1, at 1 cm depth intervals within the down-core sediment sequences. In agreement with studies in deep water settings, we find no significant intraspecies variability in either δ13C foram or δ18O foram with sediment living depth on the South Georgia shelf. Our findings add to the growing evidence that infaunal benthic foraminiferal species calcify at a fixed depth. Given the wide range of depths that we find “living” ‘infaunal’ species, we speculate that they may actually calcify predominantly at the sediment-seawater interface, where carbonate ion concentration and organic carbon availability is at a maximum

Assessment of Subjective Experiences of Boys Entering the Community From a Correctional School

The purpose of this study was to explore, classify, and evaluate the personal experiences of juvenile offenders following release from a state training school. During a two month period beginning in the fall of 1967, a group of twenty-three releasees from the MacLaren School for Boys in Woodburn, Oregon were interviewed on a weekly basis in the Portland metropolitan area. The boys, ranging in age from thirteen to seventeen were contacted in their homes, schools, and other settings to learn of positive, negative, and critical experiences encountered in the community. Open ended questions based upon a twenty- seven item schedule were administered to the boys, focusing upon personal experiences in six major areas: school, family, peer group, community, work and authority. Responses were recorded verbatim and classified according to the boys\u27 judgments of positive and negative connotations. Critical experiences were also recorded to determine specific incidents which brought the boys into contact with legal authorities or otherwise seriously affected their parole status. Positive, negative, and critical experiences from this study were analyzed both descriptively and statistically. Null hypotheses were tested to determine the quality, quantity, and intensity of experiences with respect to such major variables as age of the boys, race, length of stay in the community, records of school attendance, and personality inventory characteristics. Experiences were similarly related with respect to other variables found to be present as a result of the interviewing experience. Findings yielded evidence to indicate a predominance of negative experiences in the boys’ contacts with the community following release. The highest proportion of these occurred within the boys’ families, followed by contacts with authority and school, respectively. Peer group relationships for the boys indicated the highest proportion of positive experiences when compared with other major categories

Determinants of postnatal spleen tissue regeneration and organogenesis

Abstract The spleen is an organ that filters the blood and is responsible for generating blood-borne immune responses. It is also an organ with a remarkable capacity to regenerate. Techniques for splenic auto-transplantation have emerged to take advantage of this characteristic and rebuild spleen tissue in individuals undergoing splenectomy. While this procedure has been performed for decades, the underlying mechanisms controlling spleen regeneration have remained elusive. Insights into secondary lymphoid organogenesis and the roles of stromal organiser cells and lymphotoxin signalling in lymph node development have helped reveal similar requirements for spleen regeneration. These factors are now considered in the regulation of embryonic and postnatal spleen formation, and in the establishment of mature white pulp and marginal zone compartments which are essential for spleen-mediated immunity. A greater understanding of the cellular and molecular mechanisms which control spleen development will assist in the design of more precise and efficient tissue grafting methods for spleen regeneration on demand. Regeneration of organs which harbour functional white pulp tissue will also offer novel opportunities for effective immunotherapy against cancer as well as infectious diseases

Monte Carlo simulation of kilovolt electron transport in solids

A Monte Carlo procedure to simulate the penetration and energy loss of low¿energy electron beams through solids is presented. Elastic collisions are described by using the method of partial waves for the screened Coulomb field of the nucleus. The atomic charge density is approximated by an analytical expression with parameters determined from the Dirac¿Hartree¿Fock¿Slater self¿consistent density obtained under Wigner¿Seitz boundary conditions in order to account for solid¿state effects; exchange effects are also accounted for by an energy¿dependent local correction. Elastic differential cross sections are then easily computed by combining the WKB and Born approximations to evaluate the phase shifts. Inelastic collisions are treated on the basis of a generalized oscillator strength model which gives inelastic mean free paths and stopping powers in good agreement with experimental data. This scattering model is accurate in the energy range from a few hundred eV up to about 50 keV. The reliability of the simulation method is analyzed by comparing simulation results and experimental data from backscattering and transmission measurements

South Georgia marine productivity over the past 15 ka and implications for glacial evolution

The subantarctic islands of South Georgia are located in the Southern Ocean, and they may be sensitive to future climate warming. However, due to a lack of well-dated subantarctic palaeoclimate archives, there is still uncertainty about South Georgia’s response to past climate change. Here, we reconstruct primary productivity changes and infer Holocene glacial evolution by analysing two marine gravity cores: one near Cumberland Bay on the inner South Georgia shelf (GC673: ca. 9.5 to 0.3cal.kyrBP) and one offshore of Royal Bay on the mid-shelf (GC666: ca. 15.2cal.kyrBP to present). We identify three distinct benthic foraminiferal assemblages characterised by the dominance of Miliammina earlandi, Fursenkoina fusiformis, and Cassidulinoides parkerianus that are considered alongside foraminiferal stable isotopes and the organic carbon and biogenic silica accumulation rates of the host sediment. The M. earlandi assemblage is prevalent during intervals of dissolution in GC666 and reduced productivity in GC673. The F. fusiformis assemblage coincides with enhanced productivity in both cores. Our multiproxy analysis provides evidence that the latest Pleistocene to earliest Holocene (ca. 15.2 to 10.5cal.kyrBP) was a period of high productivity associated with increased glacial meltwater discharge. The mid–late Holocene (ca. 8 to 1cal.kyrBP), coinciding with a fall in sedimentation rates and lower productivity, was likely a period of reduced glacial extent but with several short-lived episodes of increased productivity from minor glacial readvances. The latest Holocene (from ca. 1cal.kyrBP) saw an increase in productivity and glacial advance associated with cooling temperatures and increased precipitation which may have been influenced by changes in the southwesterly winds over South Georgia. We interpret the elevated relative abundance of F. fusiformis as a proxy for increased primary productivity which, at proximal site GC673, was forced by terrestrial runoff associated with the spring–summer melting of glaciers in Cumberland Bay. Our study refines the glacial history of South Georgia and provides a more complete record of mid–late Holocene glacial readvances with robust chronology. Our results suggest that South Georgia glaciers were sensitive to modest climate changes within the Holocene

Tests of the Equivalence Principle with Neutral Kaons

We test the Principle of Equivalence for particles and antiparticles, using CPLEAR data on tagged K0 and K0bar decays into pi^+ pi^-. For the first time, we search for possible annual, monthly and diurnal modulations of the observables |eta_{+-}| and phi_{+-}, that could be correlated with variations in astrophysical potentials. Within the accuracy of CPLEAR, the measured values of |eta_{+-}| and phi_{+-} are found not to be correlated with changes of the gravitational potential. We analyze data assuming effective scalar, vector and tensor interactions, and we conclude that the Principle of Equivalence between particles and antiparticles holds to a level of 6.5, 4.3 and 1.8 x 10^{-9}, respectively, for scalar, vector and tensor potentials originating from the Sun with a range much greater than the distance Earth-Sun. We also study energy-dependent effects that might arise from vector or tensor interactions. Finally, we compile upper limits on the gravitational coupling difference between K0 and K0bar as a function of the scalar, vector and tensor interaction range.Comment: 15 pages latex 2e, five figures, one style file (cernart.csl) incorporate

Test of CPT Symmetry and Quantum Mechanics with Experimental data from CPLEAR

We use fits to recent published CPLEAR data on neutral kaon decays to $\pi^+\pi^-$ and $\pi e\nu$ to constrain the CPT--violation parameters appearing in a formulation of the neutral kaon system as an open quantum-mechanical system. The obtained upper limits of the CPT--violation parameters are approaching the range suggested by certain ideas concerning quantum gravity.Comment: 9 pages of uuencoded postscript (includes 3 figures

Radiation hardness qualification of PbWO4 scintillation crystals for the CMS Electromagnetic Calorimeter

This is the Pre-print version of the Article. The official published version can be accessed from the link below - Copyright @ 2010 IOPEnsuring the radiation hardness of PbWO4 crystals was one of the main priorities during the construction of the electromagnetic calorimeter of the CMS experiment at CERN. The production on an industrial scale of radiation hard crystals and their certification over a period of several years represented a difficult challenge both for CMS and for the crystal suppliers. The present article reviews the related scientific and technological problems encountered