Challenges and opportunities for wearable IoT forensics: TomTom Spark 3 as a case study

Wearable IoT devices like fitness trackers and smartwatches continue to create opportunities and challenges for forensic investigators in the acquisition and analysis of evidential artefacts in scenarios where such devices are a witness to a crime. However, current commercial and traditional forensic tools available to forensic investigators fall short of conducting device extraction and analysis of forensic artefacts from many IoT devices due to their heterogeneous nature. In this paper, we conduct a comprehensive forensic analysis and show artefacts of forensic value from the physical TomTom Spark 3 GPS fitness smartwatch, its companion app installed on an Android smartphone, and Bluetooth event logs located in the app’s metadata. Our forensic methodology and analysis involved the combination and use of a non-forensic tool, a commercial forensic tool, and a non-forensic manufacturer-independent analysis platform tool specifically designed for endurance athletes to identify, extract, analyze, and reconstruct user activity data in an investigative scenario. We show forensic metadata associated with the device information, past user activities, and audio files from the physical smartwatch. We recovered data associated with past user activities stored in proprietary activity files and databases maintained by the app on an Android smartphone. From the event logs, we show when user activity was synced with the app and uploaded to the device cloud storage. The results from our work provide vital references for forensic investigators to aid criminal investigations, highlight limitations of current forensic tools, and for developers of forensic tools an incentive into developing forensic software applications and tools that can decode all relevant data generated by wearable IoT devices

Anaphylaxis caused by latex surgical gloves immediately after starting surgery -A case report-

Anaphylaxis is an acute and fatal systemic allergic reaction to an allergen, and it can be an unpredictable and life-threatening cause during anesthesia. Latex is the second most common cause of anaphylaxis following the use of neuromuscular blocking agents during general anesthesia or surgery. We report on a 67-year-old male who had undergone surgery under general anesthesia without any problem but who presented with severe intraoperative anaphylaxis to latex surgical gloves. This case emphasizes the need for anesthesiologists to quickly diagnose and properly manage an allergic reaction in patients under general anesthesia

Real-time Characterization of Antibody Binding to Receptors on Living Immune Cells

Understanding molecular interactions on immune cells is crucial for drug development to treat cancer and autoimmune diseases. When characterizing molecular interactions, the use of a relevant living model system is important, as processes such as receptor oligomerization and clustering can influence binding patterns. We developed a protocol to enable time-resolved analysis of ligand binding to receptors on living suspension cells. Different suspension cell lines and weakly adhering cells were tethered to Petri dishes with the help of a biomolecular anchor molecule, and antibody binding was analyzed using LigandTracer. The protocol and assay described in this report were used to characterize interactions involving eight cell lines. Experiments were successfully conducted in three different laboratories, demonstrating the robustness of the protocol. For various antibodies, affinities and kinetic rate constants were obtained for binding to CD20 on both Daudi and Ramos B-cells, the T-cell co-receptor CD3 on Jurkat cells, and the Fcγ receptor CD32 on transfected HEK293 cells, respectively. Analyzing the binding of Rituximab to B-cells resulted in an affinity of 0.7–0.9 nM, which is similar to values reported previously for living B-cells. However, we observed a heterogeneous behavior for Rituximab interacting with B-cells, which to our knowledge has not been described previously. The understanding of complex interactions will be facilitated with the possibility to characterize binding processes in real-time on living immune cells. This provides the chance to broaden the understanding of how binding kinetics relate to biological function

Contextual adaptation of the Personnel Evaluation Standards for assessing faculty evaluation systems in developing countries: the case of Iran

<p>Abstract</p> <p>Background</p> <p>Faculty evaluations can identify needs to be addressed in effective development programs. Generic evaluation models exist, but these require adaptation to a particular context of interest. We report on one approach to such adaptation in the context of medical education in Iran, which is integrated into the delivery and management of healthcare services nationwide.</p> <p>Methods</p> <p>Using a triangulation design, interviews with senior faculty leaders were conducted to identify relevant areas for faculty evaluation. We then adapted the published checklist of the Personnel Evaluation Standards to fit the Iranian medical universities' context by considering faculty members' diverse roles. Then the adapted instrument was administered to faculty at twelve medical schools in Iran.</p> <p>Results</p> <p>The interviews revealed poor linkages between existing forms of development and evaluation, imbalance between the faculty work components and evaluated areas, inappropriate feedback and use of information in decision making. The principles of Personnel Evaluation Standards addressed almost all of these concerns and were used to assess the existing faculty evaluation system and also adapted to evaluate the core faculty roles. The survey response rate was 74%. Responses showed that the four principles in all faculty members' roles were met <it>occasionally </it>to <it>frequently</it>. Evaluation of teaching and research had the highest mean scores, while clinical and healthcare services, institutional administration, and self-development had the lowest mean scores. There were statistically significant differences between small medium and large medical schools (p < 0.000).</p> <p>Conclusion</p> <p>The adapted Personnel Evaluation Standards appears to be valid and applicable for monitoring and continuous improvement of a faculty evaluation system in the context of medical universities in Iran. The approach developed here provides a more balanced assessment of multiple faculty roles, including educational, clinical and healthcare services. In order to address identified deficiencies, the evaluation system should recognize, document, and uniformly reward those activities that are vital to the academic mission. Inclusion of personal developmental concerns in the evaluation discussion is essential for evaluation systems.</p

Post-Weaning Protein Malnutrition Increases Blood Pressure and Induces Endothelial Dysfunctions in Rats

Malnutrition during critical periods in early life may increase the subsequent risk of hypertension and metabolic diseases in adulthood, but the underlying mechanisms are still unclear. We aimed to evaluate the effects of post-weaning protein malnutrition on blood pressure and vascular reactivity in aortic rings (conductance artery) and isolated-perfused tail arteries (resistance artery) from control (fed with Labina®) and post-weaning protein malnutrition rats (offspring that received a diet with low protein content for three months). Systolic and diastolic blood pressure and heart rate increased in the post-weaning protein malnutrition rats. In the aortic rings, reactivity to phenylephrine (10−10–3.10−4 M) was similar in both groups. Endothelium removal or L-NAME (10−4 M) incubation increased the response to phenylephrine, but the L-NAME effect was greater in the aortic rings from the post-weaning protein malnutrition rats. The protein expression of the endothelial nitric oxide isoform increased in the aortic rings from the post-weaning protein malnutrition rats. Incubation with apocynin (0.3 mM) reduced the response to phenylephrine in both groups, but this effect was higher in the post-weaning protein malnutrition rats, suggesting an increase of superoxide anion release. In the tail artery of the post-weaning protein malnutrition rats, the vascular reactivity to phenylephrine (0.001–300 µg) and the relaxation to acetylcholine (10−10–10−3 M) were increased. Post-weaning protein malnutrition increases blood pressure and induces vascular dysfunction. Although the vascular reactivity in the aortic rings did not change, an increase in superoxide anion and nitric oxide was observed in the post-weaning protein malnutrition rats. However, in the resistance arteries, the increased vascular reactivity may be a potential mechanism underlying the increased blood pressure observed in this model

Biospectroscopy for Plant and Crop Science

Plants as our most renewable natural resource are indispensable within earth's biosphere, especially for food security. Providing food security in a modern world requires an ever-increasing understanding of how plants, and their products, respond to changes in the environment. In this respect, a combination of physical and chemical analytical methods can be used to study the structure and function of plants at the whole-plant, organ, tissue, cellular, and biochemical levels. Vibrational spectroscopy in biology, sometimes known as biospectroscopy, encompasses a number of techniques, among them mid-infrared and Raman spectroscopy. These techniques are well-established label-free, nondestructive, and environmentally friendly analytical methods that generate a spectral “signature” of samples using mid-infrared radiation. The resultant wavenumber spectrum containing hundreds of variables as unique as a biochemical “fingerprint” represents the biomolecules (proteins, lipids, carbohydrates, nucleic acids) present within a sample, which may serve as spectral “biomarkers” for the discrimination of distinct as well as closely related biomaterials, for various applications. In plants, biospectroscopy has been used to characterize surface structures in intact plant tissues such as leaves and fruit, plant cuticles, and cell walls, as well as to study the effects of stress on plant species. Not only does this allow the effective discrimination and “chemoidentification” of different plant structures, varieties, and cultivars, it also permits chemical profiling of plant tissues for physiological applications such as plant health monitoring and disease detection. Technical advancements are starting to overcome the major limitations of biospectroscopy such as detection sensitivity, penetration/imaging depth, and computational analysis speed, expanding the application of biospectroscopy in the plant and crop sciences. Vibrational spectra thereby serve as a basis for localization, identification, quantification of key compounds within plants, as well as to track dynamic processes for molecular-level analytics and diagnostics. This provides development potential as sensors in automatic decision-making platforms for areas including precision farming and the food production/supply chain. In this chapter we will discuss the application of biospectroscopy to study plant and crop biology and consider the potential for advancements to make biospectroscopy a more prominent technology for fundamental plant research and applied crop science as part of solutions to agricultural challenges both now and in the future