Comparison between conjugated linoleic acid and essential fatty acids in preventing oxidative stress in bovine mammary epithelial cells

Some in vitro and in vivo studies have demonstrated protective effects of conjugated linoleic acid (CLA) isomers against oxidative stress and lipid peroxidation. However, only a few and conflicting studies have been conducted showing the antioxidant potential of essential fatty acids. The objectives of the study were to compare the effects of CLA to other essential fatty acids on the thiol redox status of bovine mammary epithelia cells (BME-UV1) and their protective role against oxidative damage on the mammary gland by an in vitro study. The BME-UV1 cells were treated with complete medium containing 50 μM of cis-9,trans-11 CLA, trans-10,cis-12 CLA, α-linolenic acid, γ-linolenic acid, and linoleic acid. To assess the cellular antioxidant response, glutathione, NADPH, and γ-glutamyl-cysteine ligase activity were measured 48 h after addition of fatty acids (FA). Intracellular reactive oxygen species and malondialdehyde production were also assessed in cells supplemented with FA. Reactive oxygen species production after 3 h of H2O2 exposure was assessed to evaluate and to compare the potential protection of different FA against H2O2-induced oxidative stress. All FA treatments induced an intracellular GSH increase, matched by high concentrations of NADPH and an increase of γ-glutamyl-cysteine ligase activity. Cells supplemented with FA showed a reduction in intracellular malondialdehyde levels. In particular, CLA isomers and linoleic acid supplementation showed a better antioxidant cellular response against oxidative damage induced by H2O2 compared with other FA

An economic, perception and biophysical approach to the use of oat straw as mulch in Mediterranean rainfed agriculture land

Soil erosion is a key cause of land degradation in agriculture lands; and it is a worldwide threat that must be solved by means of nature-based strategies to be able to achieve sustainability. The use of mulches can be a solution, but there is a lack of information on long-term effects of the use of straw. Furthermore, little is known about the perception of farmers and the economic cost on the implantation of straw as a conservation measure. Eight paired plots were selected in Sierra de Enguera on an agriculture field to determine the effect of straw cover on soil erosion. Four plots were tilled three times per year (Control) and four plots were not ploughed and 0.125 kg m−2 y−1 of oat straw cover was applied yearly (Straw). The plots were established in 2002, and runoff and sediment was continuously collected after each rainfall event from 2004 till 2014 when the two managements were applied. The results show an immediate effect of the straw mulches as in these plots the runoff (from 7.7 till 5.9%) and soil erosion (from 47 till 26 Mg ha−1 y−1) was reduced already in the first year. The combined effect of the use of straw yearly and the no-tillage strategy resulted in a reduction of the sediment yield, and 11 years later soil erosion rates were two orders of magnitude lower than in the control plot. However, the perception of the farmers on the use of straw is very negative and they claim that subsidies need to be implemented, as the cost of straw mulch is 1.9 times more expensive than traditional tillage

Land degradation and mitigation policies in the mediterranean region. A brief commentary

Land degradation is more evident where conditions of environmental vulnerability already exist because of arid climate and unsustainable forms of land exploitation. Consequently, semi-arid and dry areas have been identified as vulnerable land, requiring attention from both science and policy perspectives. In some regions, such as the Mediterranean region, land degradation is particularly intense, although there are no extreme ecological conditions. In these contexts, a wide range of formal and informal responses is necessary to face particularly complex and spatially differentiated territorial processes. However, the fit of responses has been demonstrated to be different over time and space according to the underlying socioeconomic context and the specific ecological conditions. The present commentary discusses this sort of \u201centropy\u201d in the policy response to land degradation in Southern Europe, outlining the intrinsic complexity of human\u2013nature dynamics at the base of such processes. Reflecting the need of differentiated regional strategies and more specific national measures to combat desertification, three policy frameworks (agro-environmental, economic, social) with an indirect impact on fighting land degradation have been considered, delineating the importance of policy assemblages. Finally, the importance of policy impact assessment methodologies was highlighted, focusing on the possible responses reinforcing a continental strategy against land degradation. By evidencing the role of participatory planning, developmental policies indirectly addressing land degradation reveal to be an important vector of more specific measures abating desertification risk, creating, in turn, a favorable context for direct interventions of mitigation or adaptation to climate change

(-)-Epigallocatechin-3-gallate and hydroxytyrosol improved antioxidative and anti-inflammatory responses in bovine mammary epithelial cells

(-)-Epigallocatechin-3-gallate and hydroxytyrosol improved antioxidative and anti-inflammatory responses in bovine mammary epithelial cells L. Basiricò1, P. Morera1, D. Dipasquale1, R. Bernini1, L. Santi1, A. Romani2, N. Lacetera1 and U. Bernabucci1† 1Dipartimento di Scienze Agrarie e Forestali (DAFNE), Università degli Studi della Tuscia, via San Camillo de Lellis, 01100, Viterbo, Italy; 2Dipartimento di Statistica, Informatica, Applicazioni (DiSiA) “Giuseppe Parenti”, Università degli Studi di Firenze, via Morgagni 59, 50134, Firenze, Italy (-)-Epigallocatechin-3-gallate (EGCG), the major phenolic compound of green tea, and hydroxytyrosol (HTyr), a phenol found in olive oil, have received attention due to their wide-ranging health benefits. To date, there are no studies that report their effect in bovine mammary gland. Therefore, the aim of this study was to evaluate the anti-oxidative and anti-inflammatory effects of EGCG and HTyr in bovine mammary epithelial cell line (BME-UV1) and to compare their antioxidant and anti-inflammatory in vitro efficacy. Sample of EGCG was obtained from a commercially available green tea extract while pure HTyr was synthetized in our laboratories. The mammary oxidative stress and inflammatory responses were assessed by measuring the oxidative stress biomarkers and the gene expression of inflammatory cytokines. To evaluate the cellular antioxidant response, glutathione (GSH/ GSSH), γ-glutamylcysteine ligase activity, reactive oxygen species and malondialdehyde (MDA) production were measured after 48-h incubation of 50 μM EGCG or 50 μM of HTyr. Reactive oxygen species production after 3 h of hydrogen peroxide (50 μM H2O2) or lipopolysaccharide (20 μM LPS) exposure was quantified to evaluate and to compare the potential protection of EGCG and HTyr against H2O2-induced oxidative stress and LPS-induced inflammation. The anti-inflammatory activity of EGCG and HTyr was investigated by the evaluation of pro and anti-inflammatory interleukins (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and IL-10) messenger RNA abundance after treatment of cells for 3 h with 20 μM of LPS. Data were analyzed by one-way ANOVA. (-)-Epigallocatechin-3-gallate or HTyr treatments induced higher concentrations of intracellular GSH compared to control cells, matched by an increase of γ-glutamylcysteine ligase activity mainly in cells treated with HTyr. Interestingly, EGCG and HTyr prevented oxidative lipid damage in the BME-UV1 cells by a reduction of intracellular MDA levels. (-)-Epigallocatechin-3-gallate and HTyr were able to enhance cell resistance against H2O2-induced oxidative stress. It was found that EGCG and HTyr elicited a reduction of the three inflammatory cytokines TNF-α, IL-1β, IL-6 and an increase of the anti-inflammatory cytokine IL-10. Hydroxytyrosol has proved to be a strong antioxidant compound, and EGCG has shown mainly an anti-inflammatory profile. These results indicated that EGCG and HTyr may provide dual protection because they were able to attenuate oxidative stress and inflammatory responses, suggesting that these phenolic compounds are potential natural alternatives to be used in dairy cattle as feed supplement for reducing the development of oxidative and inflammatory processes related to parturition or as topical treatments for the control of bovine intramammary inflammation. Animal (2019), 13:12, pp 2847–2856Ministry for education, University and Research of Italy (MIUR) for financial support (Law 232/216, Departments of Excellence)

Policies can help to apply successful strategies to control soil and water losses. The case of chipped pruned branches (CPB) in Mediterranean citrus plantations

There is a need to devise management strategies that control soil and water losses in agriculture land to allow the design of proper policies to achieve sustainability. It is the responsibility of scientists to work with other actors to co-construct strategies that will lead to sustainable land-use policies. Using chipped pruned branches (CPB) as mulch can be a viable option because they represent local (in situ) organic material that can restore soil nutrients and organic matter. This research assesses: i) the perception of farmers towards different types of management strategies and CPB's costs; ii) the biomass yield of citrus branches and the impact of CPB on soil properties; iii) how CPB affects soil erosion and runoff generation in citrus plantations; and, iv) a discussion about how to favour the use of CPB thought successful policies. To achieve those goals we carried out: i) one-hundred interviews to assess the perception of farmers and twelve interviews to assess the economic balance of twelve land owners; ii) soil was sampled at 0-2 and 4-6 cm depths; iii) pruned material was surveyed for 40 trees; and iv) forty rainfall simulation experiments (55mmh−1) were carried out in two citrus plantations at paired sites (Control versus CPB), in La Costera District in Eastern Spain. Forty circular (0.25m2) plots were installed in four rows (4×5=20 plots) in control (CON) and CPB plots (20+20=40 plots) to perform the rainfall simulations over one hour. The cost of chipping ranged from 102 to 253 ha−1, and was related to the size of the farm. The soil quality, runoff and erosion assessment showed that CPB is a suitable strategy. CPB increased organic matter from 1.3% to 2.9% after 10 years in the 0-2 cm depth layer, while the 4-6 cm layer was largely not affected (OM moved from 1.1 to 1.3% after 10 years), and soil bulk density showed a similar trend: a decrease from 1.36 to 1.16 g cm−3 in the surface layer with no change in the subsurface layer. The hydrological and erosional responses were different between CON and CPB. The CON plots initiated ponding (40 s) and runoff (107 s) earlier than the CPB plots (169 and 254 s, respectively); and runoff discharge was 60% in CON vs 43% in CPB plots. Sediment concentration was four times larger in the CON plots than in the CPB (11.3 g l−1 vs 3 g l−1), and soil erosion was 3.8 Mg ha−1 h-1 vs 0.7 Mg ha−1 h−1. CPB mulches were effective at controlling soil and water losses in Mediterranean citrus plantations as they showed the relationship between vegetation/litter cover and soil erosion rates. However, the farmer's perception survey showed that the use of CPB was not welcomed nor accepted by the farmers. Policies that aim to promote CPB as soil conservation mulch need to be promoted by subsidies as the farmers requested, and by education to demonstrate the positive effects of CPB to of the farming community

Integrating Dehumanization and Attachment in the Prediction of Teen Dating Violence Perpetration

Dehumanization has the potential to account for different abusive behaviors because it involves making negative judgments of others that make it easier to harm them. However, research has not resorted to this mechanism to analyze teen dating violence (TDV) perpetration, nor has it examined its association with the broader representations of others linked to attachment. The first objective of this study was to analyze whether dehumanization of one’s partner (lesser perceived agency and experience) and attribution of evilness were associated with a higher level of TDV perpetration and specific attachment styles. The second objective was to develop a structural equation model (SEM) that allowed integration of the links between all these factors. Participants in this cross-sectional study were 1799 adolescents who completed a survey in high schools. The results showed that those who were classified as high in TDV perpetration did perceive lower agency, lower experience, and higher evilness in their partners. The dehumanized perception of one’s partner was found to vary according to the attachment styles, with those highest in avoidance (dismissive and fearful) dehumanizing their partners the most. The SEM showed that dehumanization is related to avoidant and not to anxious attachment. It also pointed to the relevance of attribution of evilness in predicting TDV perpetration. The invariance of the model was tested across gender subsamples. These findings allow better understanding of how violence may arise in early relationships and where to focus intervention with adolescent

La agricultura ecológica a largo plazo en plantaciones de cítricos permite la recuperación del carbono orgánico del suelo

[ES] Se ha demostrado que el manejo del suelo bajo agricultura ecológica puede aumentar el contenido de carbono orgánico en el suelo moderando el incremento de los gases de efecto invernadero, pero hasta la fecha las evaluaciones cuantitativas basadas en mediciones a largo plazo han sido escasas, especialmente bajo condiciones mediterráneas. En esta investigación se examinaron los cambios en el contenido de carbono orgánico como respuesta a la agricultura ecológica con cobertura vegetal en una plantación de cítricos en el área mediterránea, utilizando una base de datos de 21 años. El incremento de contenido de carbono orgánico en el suelo fue más evidente tras cinco años desde el cambio del manejo del suelo, sugiriendo que, para plantaciones de cítricos en ambientes mediterráneos, los estudios deberían tener una duración superior a cinco años. La sata de secuestro de carbono orgánico no cambió significativamente durante los 21 años de observaciones, con valores que oscilaron entre -1.10 Mg C ha-1 a-1 y 1.89 Mg C ha-1 a-1. Tras 21 años, un total de 61 t CO2 ha-1 fueron secuestradas en las áreas de acumulación de carbono en el suelo. Estos resultados demuestran que la agricultura ecológica es una estrategia efectiva para restaurar o incrementar los niveles de carbono orgánico en el suelo en los sistemas de cítricos mediterráneos.[EN] It has been shown that soil management under organic farming can enhance soil organic carbon, thereby mitigating atmospheric greenhouse gas increases, but until now quantitative evaluations based on long term experiments are scarce, especially under Mediterranean conditions. Changes in soil organic carbon (SOC) content were examined in response to organic management with cover crops in a Mediterranean citrus plantation using 21 years of survey data. Soil organic carbon increase was more apparent 5 years after a land management change suggesting that, for citrus plantations on Mediterranean conditions, studies should be longer than five years in duration. Soil organic carbon sequestration rate did not significantly change during the 21 years of observation, with values ranging from -1.10 Mg C ha(-1) y(-1) to 1.89 Mg C ha(-1) y(-1). After 21 years, 61 Mg CO2 ha(-1) were sequestered in long-lived soil C pools. These findings demonstrate that organic management is an effective strategy to restore or increase SOC content in Mediterranean citrus systems.This research was funded by the European Union Seventh Framework Program (FP7/2007-2013) under grant no. 603498 (RECARE Project) and the research projects GL2008-02879/BTE and LEDDRA 243857.Novara, A.; Pulido, M.; Rodrigo-Comino, J.; Di Prima, S.; Smith, P.; Gristina, L.; Giménez Morera, A.... (2019). Long-term organic farming on a citrus plantation results in soil organic carbon recovery. Cuadernos de Investigación Geográfica. 45(1):271-286. https://doi.org/10.18172/cig.3794S27128645

Infrastructure and equipment for radiation oncology in the spanish national health system: analysis of external beam radiotherapy 2015-2020.

PURPOSE Planning for radiation oncology requires reliable estimates of both demand for radiotherapy and availability of technological resources. This study compares radiotherapy resources in the 17 regions of the decentralised Spanish National Health System (SNHS). MATERIAL AND METHODS The Sociedad Española de Oncología Radioterápica (SEOR) performed a cross-sectional survey of all Spanish radiation oncology services (ROS) in 2015. We collected data on SNHS radiotherapy units, recording the year of installation, specific features of linear accelerators (LINACs) and other treatment units, and radiotherapeutic techniques implemented by region. Any machine over 10 years old or lacking a multileaf collimator or portal imaging system was considered obsolete. We performed a k-means clustering analysis using the Hartigan-Wong method to test associations between the gross domestic regional product (GDRP), the number of LINACs per million population and the percentage of LINACs over 10 years old. RESULTS The SNHS controls 72 (61%) of the 118 Spanish ROS and has 180 LINACs, or 72.5% of the total public and private resources. The mean rate of LINACs per million population is 3.9 for public ROS, and 42% (n=75) of the public accelerators were obsolete in 2015: 61 due to age and 14 due to technological capability. There was considerable regional variation in terms of the number and technological capacity of radiotherapy units; correlation between GRDP and resource availability was moderate. CONCLUSION Despite improvements, new investments are still needed to replace obsolete units and increase access to modern radiotherapy. Regular analysis of ROS in each Spanish region is the only strategy for monitoring progress in radiotherapy capacity.pre-print1471 K

Screening for coping style increases the power of gene expression studies

Background: Individuals of many vertebrate species show different stress coping styles and these have a striking influence on how gene expression shifts in response to a variety of challenges. Principal Findings: This is clearly illustrated by a study in which common carp displaying behavioural predictors of different coping styles (characterised by a proactive, adrenaline-based or a reactive, cortisol-based response) were subjected to inflammatory challenge and specific gene transcripts measured in individual brains. Proactive and reactive fish differed in baseline gene expression and also showed diametrically opposite responses to the challenge for 80% of the genes investigated. Significance: Incorporating coping style as an explanatory variable can account for some the unexplained variation that is common in gene expression studies, can uncover important effects that would otherwise have passed unnoticed and greatly enhances the interpretive value of gene expression data

Gla Rich Protein (GRP) is associated to osteoarthritis being highly accumulated in the joint tissues and synovial fluid

info:eu-repo/semantics/publishedVersio