54 research outputs found
Performance and application of an open source automated magnetic optical density meter for analyzing magnetotactic bacteria
We present a spectrophotometer (optical density meter) combined with
electromagnets dedicated to the analysis of magnetotactic bacteria. We have
ensured that our system, called MagOD, can be easily reproduced by providing
the source of the 3D prints for the housing, electronic designs, circuit board
layouts, and microcontroller software. We compare the performance of this novel
system to existing adapted commercial spectrophotometers. In addition, we
demonstrate its use by analyzing the absorbance of magnetotactic bacteria as a
function of their orientation with respect to the light path and their speed of
reorientation after the field has been rotated by 90o. We continuously
monitored the development of a culture of magnetotactic bacteria over a period
of five days, and measured the development of their velocity distribution over
a period of one hour. Even though this dedicated spectrophotometer is
relatively simple to construct and cost-effective, a range of magnetic
field-dependent parameters can be extracted from suspensions of magnetotactic
bacteria. Therefore, this instrument will help the magnetotactic research
community to understand and apply this intriguing micro-organism
X20CoCrWMo10-9//Co3O4: a Metal-Ceramic Composite with Unique Efficiency Values for Water-Splitting in Neutral Regime
Water splitting allows the storage of solar energy into chemical bonds
(H2+O2) and will help to implement the urgently needed replacement of limited
available fossil fuels. Particularly in neutral environment electrochemically
initiated water splitting suffers from low efficiency due to high
overpotentials caused by the anode. Electro-activation of X20CoCrWMo10-9, a
Co-based tool steel resulted in a new composite material
(X20CoCrWMo10-9//Co3O4) that catalyzes the anode half-cell reaction of water
electrolysis with a so far unequalled effectiveness. The current density
achieved with this new anode in pH 7 corrected 0.1 M phosphate buffer is over a
wide range of overpotentials around 10 times higher compared to recently
developed, up-to-date electrocatalysts and represents the benchmark performance
advanced catalysts show in regimes that support water splitting significantly
better than pH 7 medium. X20CoCrWMo10-9//Co3O4 exhibited electrocatalytic
properties not only at pH 7, but also at pH 13, which is much superior to the
ones of IrO2-RuO2, single-phase Co3O4- or Fe/Ni- based catalysts. Both XPS and
FT-IR experiments unmasked Co3O4 as the dominating compound on the surface of
the X20CoCrWMo10-9//Co3O4 composite. Upon a comprehensive dual beam FIB-SEM
(focused ion beam-scanning electron microscopy) study we could show that the
new composite does not exhibit a classical substrate-layer structure due to the
intrinsic formation of the Co-enriched outer zone. This structural
particularity is basically responsible for the outstanding electrocatalytic OER
performance
An open-source automated magnetic optical density meter for analysis of suspensions of magnetic cells and particles
We present a spectrophotometer (optical density meter) combined with electromagnets dedicated to the analysis of suspensions of magnetotactic bacteria. The instrument can also be applied to suspensions of other magnetic cells and magnetic particles. We have ensured that our system, called MagOD, can be easily reproduced by providing the source of the 3D prints for the housing, electronic designs, circuit board layouts, and microcontroller software. We compare the performance of our system to existing adapted commercial spectrophotometers. In addition, we demonstrate its use by analyzing the absorbance of magnetotactic bacteria as a function of their orientation with respect to the light path and their speed of reorientation after the field has been rotated by 90°. We continuously monitored the development of a culture of magnetotactic bacteria over a period of 5 days and measured the development of their velocity distribution over a period of one hour. Even though this dedicated spectrophotometer is relatively simple to construct and cost-effective, a range of magnetic field-dependent parameters can be extracted from suspensions of magnetotactic bacteria. Therefore, this instrument will help the magnetotactic research community to understand and apply this intriguing micro-organism
Gestational weight gain charts for different body mass index groups for women in Europe, North America, and Oceania
BackgroundGestational weight gain differs according to pre-pregnancy body mass index and is related to the risks of adverse maternal and child health outcomes. Gestational weight gain charts for women in different pre-pregnancy body mass index groups enable identification of women and offspring at risk for adverse health outcomes. We aimed to construct gestational weight gain reference charts for underweight, normal weight, overweight, and grades 1, 2 and 3 obese women and to compare these charts with those obtained in women with uncomplicated term pregnancies.MethodsWe used individual participant data from 218,216 pregnant women participating in 33 cohorts from Europe, North America, and Oceania. Of these women, 9065 (4.2%), 148,697 (68.1%), 42,678 (19.6%), 13,084 (6.0%), 3597 (1.6%), and 1095 (0.5%) were underweight, normal weight, overweight, and grades 1, 2, and 3 obese women, respectively. A total of 138, 517 women from 26 cohorts had pregnancies with no hypertensive or diabetic disorders and with term deliveries of appropriate for gestational age at birth infants. Gestational weight gain charts for underweight, normal weight, overweight, and grade 1, 2, and 3 obese women were derived by the Box-Cox t method using the generalized additive model for location, scale, and shape.ResultsWe observed that gestational weight gain strongly differed per maternal pre-pregnancy body mass index group. The median (interquartile range) gestational weight gain at 40weeks was 14.2kg (11.4-17.4) for underweight women, 14.5kg (11.5-17.7) for normal weight women, 13.9kg (10.1-17.9) for overweight women, and 11.2kg (7.0-15.7), 8.7kg (4.3-13.4) and 6.3kg (1.9-11.1) for grades 1, 2, and 3 obese women, respectively. The rate of weight gain was lower in the first half than in the second half of pregnancy. No differences in the patterns of weight gain were observed between cohorts or countries. Similar weight gain patterns were observed in mothers without pregnancy complications.ConclusionsGestational weight gain patterns are strongly related to pre-pregnancy body mass index. The derived charts can be used to assess gestational weight gain in etiological research and as a monitoring tool for weight gain during pregnancy in clinical practice.Peer reviewe
Crystal-chemical and biological controls of trace and minor element incorporation into magnetite nanocrystals
Magnetite nanoparticles possess numerous fundamental, biomedical and industrial applications, many of which depend on tuning the magnetic properties. This is often achieved by the incorporation of trace and minor elements into the magnetite lattice. Such incorporation was shown to depend strongly on the magnetite formation pathway (i.e., abiotic vs biological), but the mechanisms controlling element partitioning between magnetite and its surrounding precipitation solution remain to be elucidated. Here, we used a combination of theoretical modelling (lattice and crystal field theories) and experimental evidence (high-resolution inductively coupled plasma mass spectrometry and X-ray absorption spectroscopy) to demonstrate that element incorporation into abiotic magnetite nanoparticles is controlled principally by cation size and valence. Elements from the first series of transition metals (Cr to Zn) constituted exceptions to this finding as their incorporation appeared to be also controlled by the energy levels of their unfilled 3d orbitals, in line with crystal field mechanisms. We then show that element incorporation into biological magnetite nanoparticles produced by magnetotactic bacteria (MTB) cannot be explained by crystal-chemical parameters alone, which points to the biological control exerted by the bacteria over the element transfer between MTB growth medium and the intracellular environment. This screening effect generates biological magnetite with a purer chemical composition than the abiotic materials formed in a solution of similar composition. Our work establishes a theoretical framework for understanding the crystal-chemical and biological controls of trace and minor cation incorporation into magnetite, thereby providing predictive methods to tailor the composition of magnetite nanoparticles for improved control over magnetic properties
Crystal-chemical and biological controls of trace and minor element incorporation into magnetite nanocrystals
Magnetite nanoparticles possess numerous fundamental, biomedical and industrial applications, many of which depend on tuning the magnetic properties. This is often achieved by the incorporation of trace and minor elements into the magnetite lattice. Such incorporation was shown to depend strongly on the magnetite formation pathway (i.e., abiotic vs biological), but the mechanisms controlling element partitioning between magnetite and its surrounding precipitation solution remain to be elucidated. Here, we used a combination of theoretical modelling (lattice and crystal field theories) and experimental evidence (high-resolution inductively coupled plasma mass spectrometry and X-ray absorption spectroscopy) to demonstrate that element incorporation into abiotic magnetite nanoparticles is controlled principally by cation size and valence. Elements from the first series of transition metals (Cr to Zn) constituted exceptions to this finding as their incorporation appeared to be also controlled by the energy levels of their unfilled 3d orbitals, in line with crystal field mechanisms. We then show that element incorporation into biological magnetite nanoparticles produced by magnetotactic bacteria (MTB) cannot be explained by crystal-chemical parameters alone, which points to the biological control exerted by the bacteria over the element transfer between MTB growth medium and the intracellular environment. This screening effect generates biological magnetite with a purer chemical composition than the abiotic materials formed in a solution of similar composition. Our work establishes a theoretical framework for understanding the crystal-chemical and biological controls of trace and minor cation incorporation into magnetite, thereby providing predictive methods to tailor the composition of magnetite nanoparticles for improved control over magnetic properties
Bonding Properties of FCC-like Au44(SR)28 Clusters from X-ray Absorption Spectroscopy
Thiolate-protected gold clusters with precisely controlled atomic composition have recently emerged as promising candidates for a variety of applications because of their unique optical, electronic and catalytic properties. The recent discovery of the Au44(SR)28 total structure is considered as an interesting finding in terms of the face-centered cubic (FCC)-like core structure in small goldâ thiolate clusters. Herein, the unique bonding properties of Au44(SR)28 is analyzed using temperature-dependent X-ray absorption spectroscopy (XAS) measurements at the Au L3-edge, and compared with other FCC-like clusters such as Au36(SR)24 and Au28(SR)20. A negative thermal expansion was detected for the Au-Au bonds of the metal core (the first Au-Au shell), and was interpreted based on the unique Au core structure consisting of the Au4 units. EXAFS fitting results from Au28(SR)20, Au36(SR)24 and Au44(SR)28 show a size dependent negative thermal expansion behavior in the first Au-Au shell, further highlighting the importance of the Au4 units in determining the Au core bonding properties, and shedding lights on the growth mechanism of these FCC-like Au clusters.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
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