104 research outputs found
Analysis of aroma compounds and nutrient contents of mabolo (Diospyros blancoi A. DC.), an ethnobotanical fruit of Austronesian Taiwan
AbstractDiospyros blancoi A. DC. is an evergreen tree species of high-quality wood. Mabolo, the fruit of this plant, is popular among the natives in Taiwan, but its potential in economic use has not been fully explored. Mabolo has a rich aroma. Of the 39 different volatile compounds isolated, its intact fruit and peel were found to both contain 24 compounds, whereas the pulp contained 28 compounds. The most important aroma compounds were esters and α-farnesene. Our data show that mabolo is rich in dietary fiber (3.2%), and the contents of other nutrients such as malic acid, vitamin B2, vitamin B3, folic acid, pantothenic acid, and choline chloride were 227.1 mg/100 g, 0.075 mg/100 g, 0.157 mg/100 g, 0.623 mg/100 g, 0.19 mg/100 g, and 62.52 mg/100 g, respectively. Moreover, it is rich in calcium and zinc; the contents of which were found to be 42.8 mg/100 g and 3.6 mg/100 g, respectively. Our results show that D. blancoi has the potential to be bred for a novel fruit
Development of pollen mediated activation tagging system for Phalaenopsis and Doritaenopsis
Abstract In the present study, a novel plant transformation system for
Doritaenopsis and Phalaenopsis has been developed. The
pollen-mediated activation tagging system was established by artificial
pollination. The pollens, co-cultured with Agrobacterium tumefaciens
strain EHA105 harbouring an activation tagging vector (pTAG-8), were
used for pollination. In order to optimize the transformation
efficiency, several factors (concentration of A. tumefaciens,
concentration of acetosyringone during co-cultivation and the duration
of co-cultivation) known to influence Agrobacterium-mediated DNA
transfer were examined. A concentration of 0.5-1 x 108 CFU/ml for A.
tumefaciens, 0.1 mM acetosyringone, and 6 hrs of co-culture period were
found to be the optimal condition for high transformation efficiency.
Integration of T-DNA into the genome of putative transgenic plants was
confirmed by PCR and DNA blot analyses. Single copy of the transgene
was observed in all transgenic plants analyzed. Most of the transgenic
plants had a morphologically normal phenotype and the overall capsule
formation efficiency was similar to control plant. Our results showed a
new approach of genetic transformation in orchids and this method can
be employed for genetic improvement of the orchids
An ALMA Study of the FU Ori─type Object V900 Mon: Implications for the Progenitor
We present ALMA observations of 12CO, 13CO, and C18O J = 2─1 lines and the 230 GHz continuum for the F Ori─type object (FUor) V900 Mon (d ∼ 1.5 kpc), for which the accretio burst was triggered between 1953 and 2009. We identified CO emissio associated with a molecular bipolar outflow extending up to a ∼104 au scale and a rotating molecular envelope extendin over >104 au. The interaction with the hot energetic FUo wind, which was observed using optical spectroscopy, appears limited t a region within ∼400 au of the star. The envelope mass and collimatio of the extended CO outflow suggest that the progenitor of this FUor is low-mass Class I young stellar object (YSO). These parameters for V90 Mon, another FUor, and a few FUor-like stars are consistent with th idea that FUor outbursts are associated with normal YSOs. The continuu emission is marginally resolved in our observations with a 0.″2 × 0.″1 (∼300 × 225 au) beam, and a Gaussian model provides a deconvolved FWH of ∼90 au. The emission is presumably associated with a dust circumstellar disk, plus a possible contribution from a wind or win cavity close to the star. The warm compact nature of the disk continuu emission could be explained with viscous heating of the disk, whil gravitational fragmentation in the outer disk and/or a combination o grain growth and their inward drift may also contribute to its compac nature
PmoB subunit of particulate methane monooxygenase (pMMO) in Methylococcus capsulatus (Bath): The Cu^I sponge and its function
In this study, we describe efforts to clarify the role of the copper cofactors associated with subunit B (PmoB) of the particulate methane monooxygenase (pMMO) from Methylococcus capsulatus (Bath) (M. capsulatus). This subunit exhibits strong affinity toward Cu^I ions. To elucidate the high copper affinity of the subunit, the full-length PmoB, and the N-terminal truncated mutants PmoB_(33–414) and PmoB_(55–414), each fused to the maltose-binding protein (MBP), are cloned and over-expressed into Escherichia coli (E. coli) K12 TB1 cells. The Y374F, Y374S and M300L mutants of these protein constructs are also studied. When this E. coli is grown with the pmoB gene in 1.0 mM Cu^(II), it behaves like M. capsulatus (Bath) cultured under high copper stresswith abundant membrane accumulation and high CuI content. The recombinantPmoB proteins are verified by Western blotting of antibodies directed against the MBP sub-domain in each of the copper-enriched PmoB proteins. Cu K-edge X-rayabsorption near edge spectroscopy (XANES) of the copper ions confirms that all the PmoB recombinants are Cu^I proteins. All the PmoB proteins show evidence of a “dicopper site” according to analysis of the Cu extended X-ray absorption edge fine structure (EXAFS) of the membranes. No specific activities toward methane and propene oxidation are observed with the recombinant membrane-bound PmoB proteins. However, significant production of hydrogen peroxide is observed in the case of the PmoB_(33–414) mutant. Reaction of the dicopper site with dioxygenproduces hydrogen peroxide and leads to oxidation of the CuI ions residing in the C-terminal sub-domain of the PmoB subunit
PmoB subunit of particulate methane monooxygenase (pMMO) in Methylococcus capsulatus (Bath): The Cu^I sponge and its function
In this study, we describe efforts to clarify the role of the copper cofactors associated with subunit B (PmoB) of the particulate methane monooxygenase (pMMO) from Methylococcus capsulatus (Bath) (M. capsulatus). This subunit exhibits strong affinity toward Cu^I ions. To elucidate the high copper affinity of the subunit, the full-length PmoB, and the N-terminal truncated mutants PmoB_(33–414) and PmoB_(55–414), each fused to the maltose-binding protein (MBP), are cloned and over-expressed into Escherichia coli (E. coli) K12 TB1 cells. The Y374F, Y374S and M300L mutants of these protein constructs are also studied. When this E. coli is grown with the pmoB gene in 1.0 mM Cu^(II), it behaves like M. capsulatus (Bath) cultured under high copper stresswith abundant membrane accumulation and high CuI content. The recombinantPmoB proteins are verified by Western blotting of antibodies directed against the MBP sub-domain in each of the copper-enriched PmoB proteins. Cu K-edge X-rayabsorption near edge spectroscopy (XANES) of the copper ions confirms that all the PmoB recombinants are Cu^I proteins. All the PmoB proteins show evidence of a “dicopper site” according to analysis of the Cu extended X-ray absorption edge fine structure (EXAFS) of the membranes. No specific activities toward methane and propene oxidation are observed with the recombinant membrane-bound PmoB proteins. However, significant production of hydrogen peroxide is observed in the case of the PmoB_(33–414) mutant. Reaction of the dicopper site with dioxygenproduces hydrogen peroxide and leads to oxidation of the CuI ions residing in the C-terminal sub-domain of the PmoB subunit
Shot Noise in Mesoscopic Conductors
Theoretical and experimental work concerned with dynamic fluctuations has
developed into a very active and fascinating subfield of mesoscopic physics. We
present a review of this development focusing on shot noise in small electric
conductors. Shot noise is a consequence of the quantization of charge. It can
be used to obtain information on a system which is not available through
conductance measurements. In particular, shot noise experiments can determine
the charge and statistics of the quasiparticles relevant for transport, and
reveal information on the potential profile and internal energy scales of
mesoscopic systems. Shot noise is generally more sensitive to the effects of
electron-electron interactions than the average conductance. We present a
discussion based on the conceptually transparent scattering approach and on the
classical Langevin and Boltzmann-Langevin methods; in addition a discussion of
results which cannot be obtained by these methods is provided. We conclude the
review by pointing out a number of unsolved problems and an outlook on the
likely future development of the field.Comment: 99 two-column pages; 38 .eps figures included. Submitted to Physics
Reports. Many minor improvements; typos corrected; references added and
update
Shot noise in mesoscopic systems
This is a review of shot noise, the time-dependent fluctuations in the
electrical current due to the discreteness of the electron charge, in small
conductors. The shot-noise power can be smaller than that of a Poisson process
as a result of correlations in the electron transmission imposed by the Pauli
principle. This suppression takes on simple universal values in a symmetric
double-barrier junction (suppression factor 1/2), a disordered metal (factor
1/3), and a chaotic cavity (factor 1/4). Loss of phase coherence has no effect
on this shot-noise suppression, while thermalization of the electrons due to
electron-electron scattering increases the shot noise slightly. Sub-Poissonian
shot noise has been observed experimentally. So far unobserved phenomena
involve the interplay of shot noise with the Aharonov-Bohm effect, Andreev
reflection, and the fractional quantum Hall effect.Comment: 37 pages, Latex, 10 figures (eps). To be published in "Mesoscopic
Electron Transport," edited by L. P. Kouwenhoven, G. Schoen, and L. L. Sohn,
NATO ASI Series E (Kluwer Academic Publishing, Dordrecht
The Overseeing Mother: Revisiting the Frontal-Pose Lady in the Wu Family Shrines in Second Century China
Located in present-day Jiaxiang in Shandong province, the Wu family shrines built during the second century in the Eastern Han dynasty (25–220) were among the best-known works in Chinese art history. Although for centuries scholars have exhaustively studied the pictorial programs, the frontal-pose female image situated on the second floor of the central pavilion carved at the rear wall of the shrines has remained a question. Beginning with the woman’s eyes, this article demonstrates that the image is more than a generic portrait (“hard motif ”), but rather represents “feminine overseeing from above” (“soft motif ”). This synthetic motif combines three different earlier motifs – the frontal-pose hostess enjoying entertainment, the elevated spectator, and the Queen Mother of the West. By creatively fusing the three motifs into one unity, the Jiaxiang artists lent to the frontal-pose lady a unique power: she not only dominated the center of the composition, but also, like a divine being, commanded a unified view of the surroundings on the lofty building, hence echoing the political reality of the empress mother’s “overseeing the court” in the second century during Eastern Han dynasty
Eye-in-Hand Robotic Arm Gripping System Based on Machine Learning and State Delay Optimization
This research focused on using RGB-D images and modifying an existing machine learning network architecture to generate predictions of the location of successfully grasped objects and to optimize the control system for state delays. A five-finger gripper designed to mimic the human palm was tested to demonstrate that it can perform more delicate missions than many two- or three-finger grippers. Experiments were conducted using the 6-DOF robot arm with the five-finger and two-finger grippers to perform at least 100 actual machine grasps, and compared to the results of other studies. Additionally, we investigated state time delays and proposed a control method for a robot manipulator. Many studies on time-delay systems have been conducted, but most focus on input and output delays. One reason for this emphasis is that input and output delays are the most commonly occurring delays in physical or electronic systems. An additional reason is that state delays increase the complexity of the overall control system. Finally, it was demonstrated that our network can perform as well as a deep network architecture with little training data and omitting steps, such as posture evaluation, and when combined with the hardware advantages of the five-finger gripper, it can produce an automated system with a gripping success rate of over 90%. This paper is an extended study of the conference paper
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