Thermographic measurement of canopy temperature is a useful tool for predicting water deficit effects on fruit weight in citrus trees

[EN] Alternative and more practical methods for plant water stress detection than stem water potential (ψs) and stomatal conductance (gs) are needed when regulated deficit irrigation (RDI) strategies are applied. The aim of this experiment was to compare sap flow and canopy temperature (Tc) measurements with more classical methods like ψs or gs to predict the effect of deficit irrigation on fresh fruit weight in citrus trees. The experiment was performed during the summer of 2011 in a “Clementina de Nules” orchard undergoing RDI. Sap flow was determined by means of the compensation heat pulse method in well-watered and RDI trees. Tc was measured continuously with infrared thermometers (IRTs) mounted over the canopies and also weekly with an infrared hand-operated thermographic camera taking frontal images of the sunlit side of tree crowns. Concurrently, ψs and gs were also measured on all trees. Results showed that the evolution of the relative transpiration obtained with the sap flow sensors was in agreement with the plant water stress experienced. The values of Tc obtained with the fixed IRTs, normalized by air temperature (Tc − Ta) were in general poorly related with ψs and gs. However, when Tc was obtained from thermal imaging, there was a good correlation with ψs in days of relatively high water stress (i.e. when ψs differences among treatments were >1.0 MPa). The average fruit weight at harvest was significantly correlated with all the stress indicators, and the best correlation was that of thermographic Tc followed by ψs and gs. Overall, results showed that in citrus trees Tc measurement obtained from thermal imaging is a good tool to predict the effect of water deficit on fresh fruit weight.The authors thank H. Hernandez and T. Yeves for their help with the field work. This experiment has been funded by the RIDECO-CONSOLIDER CSD2006-00067 and Telerieg Interreg IV Sudoe projects. C. Ballester was holder of a predoctoral fellowship FPI-INIA-CCAA. D.S. Intrigliolo acknowledges the financial support received from the Spanish Ministry of Economy and Competitiveness (MINECO) program "Ramon y Cajal".Ballester, C.; Castel, J.; Jiménez Bello, MÁ.; Castel Sánchez, JR.; Intrigliolo Molina, DS. (2013). Thermographic measurement of canopy temperature is a useful tool for predicting water deficit effects on fruit weight in citrus trees. Agricultural Water Management. 122:1-6. https://doi.org/10.1016/j.agwat.2013.02.005S1612

Development and validation of an automatic thermal imaging process forassessing plant water status

[EN] Leaf temperature is a physiological trait that can be used for monitoring plant water status. Nowadays, by means of thermography, canopy temperature can be remotely determined. In this sense, it is crucial to automatically process the images. In the present work, a methodology for the automatic analysis of frontal images taken on individual trees was developed. The procedure can be used when cameras take at the same time thermal and visible scenes, so it is not necessary to reference the images. In this way, during the processing in batch, no operator participated. The procedure was developed by means of a non supervised classification of the visible image from which the presence of sky and soil could be detected. In case of existence, a mask was performed for the extraction of intermediate pixels to calculate canopy temperature by means of the thermal image. At the same time, sunlit and shady leaves could be detected and isolated. Thus, the procedure allowed to separately determine canopy temperature either of the more exposed part of the canopy or of the shaded portion. The methodology developed was validated using images taken in several regulated deficit irrigation trials in Persimmon and two citrus cultivars (Clementina de Nules and Navel Lane-Late). Overall, results indicated that similar canopy temperatures were calculated either by means of the automatic process or the manual procedure. The procedure developed allows to drastically reduce the time needed for image analysis also considering that no operator participation was required. This tool will facilitate further investigations in course for assessing the feasibility of thermography for detecting plant water status in woody perennial crops with discontinuous canopies. Preliminary results reported indicate that the type of crop evaluated has an important influence in the results obtained from thermographic imagery. Thus, in Persimmon trees there were good correlations between canopy temperature and plant water status while, in Clementina de Nules and Navel Lane-Late citrus cultivars canopy temperature differences among trees could not be related with tree-to-tree variations in plant water status.This research was supported by funds from the Instituto Valenciano de Investigaciones Agrarias and the "Denominacion de origen Caqui Ribera del Xuquer" via "Proyecto Integral Caqui". from projects Rideco-Consolider CSD2006-0067 and Interreg IV Sudoe Telerieg. Thanks are also due to J. Castel, E. Badal, I. Buesa and D. Guerra for assistance with field work and to the Servicio de Tecnologia del Riego for providing the meteorological data.Jiménez Bello, MÁ.; Ballester, C.; Castel Sanchez, R.; Intrigliolo Molina, DS. (2011). Development and validation of an automatic thermal imaging process forassessing plant water status. Agricultural Water Management. (98):1497-1504. https://doi.org/10.1016/j.agwat.2011.05.002S149715049

Usefulness of thermography for plant water stress detection in citrus and persimmon trees

[EN] The feasibility of using canopy temperature (Tc) measured with a hand-operated infrared thermographic camera as a water stress indicator was evaluated in the field during two seasons on citrus and persimmon trees subjected to different levels of deficit irrigation. In both species, which differ in leaf anatomy and stomatal response to environmental conditions, Tc was compared with midday stem water potential (s) measurements. In persimmon trees, leaf stomatal conductance (gs) was also measured. In 2009, images were taken from the sunlit and shady sides of the canopies. Based on the results obtained, during the second experimental season images were taken from the sunlit side of the trees and also from above the canopy. In persimmon, trees under deficit irrigation had lower s and gs what resulted in a clear increase in Tc regardless of the position from where the pictures were taken. The maximum Tc difference between deficit-irrigated and control trees observed was of 4.4 ◦C, which occurred when the stressed trees hads values 1.1 MPa lower than the control ones. In persimmon trees, Tc was the most sensitive indicator of plant water status particularly due to the lower tree-to-tree variability as compared to s and gs. On the other hand, in citrus trees Tc was not always affected by plant water stress. Only in the second experimental season, when air vapour pressure deficit values were below 2.7 kPa and images were also taken from above the canopies, deficit-irrigated trees had higher Tc than the control ones, this difference being at most 1.7 ◦C. Overall, the results show that hand-operated thermographic cameras can be used to detect plant water stress in both fruit tree species. Nevertheless, the use of Tc measurements to detect plant water stress appears to be more precise in persimmon than in orange citrus. This might be because persimmon trees have larger leaf size which determines higher canopy resistance allowing for higher increases in canopy temperature in response to water stress via stomatal closure. © 2012 Elsevier B.V. All rights reserved.The authors thank E. Badal, I. Buesa, J. Castel, D. Guerra, D. Perez, F. Sanz and A. Yeves, for their help in field work. This research was supported by funds from projects RIDECO-CONSOLIDER CSD2006-00067, Telerieg Interreg IV Sudoe and "Proyecto Integral Caqui". D.S. Intrigliolo acknowledges the financial support received from the Spanish Ministry of Economy and Competitiveness (MINECO) program "Ramon y Cajal".Ballester, C.; Jiménez Bello, MÁ.; Castel, JR.; Intrigliolo, DS. (2013). Usefulness of thermography for plant water stress detection in citrus and persimmon trees. Agricultural and Forest Meteorology. 168:120-129. https://doi.org/10.1016/j.agrformet.2012.08.005S12012916

The ANTARES Optical Beacon System

ANTARES is a neutrino telescope being deployed in the Mediterranean Sea. It consists of a three dimensional array of photomultiplier tubes that can detect the Cherenkov light induced by charged particles produced in the interactions of neutrinos with the surrounding medium. High angular resolution can be achieved, in particular when a muon is produced, provided that the Cherenkov photons are detected with sufficient timing precision. Considerations of the intrinsic time uncertainties stemming from the transit time spread in the photomultiplier tubes and the mechanism of transmission of light in sea water lead to the conclusion that a relative time accuracy of the order of 0.5 ns is desirable. Accordingly, different time calibration systems have been developed for the ANTARES telescope. In this article, a system based on Optical Beacons, a set of external and well-controlled pulsed light sources located throughout the detector, is described. This calibration system takes into account the optical properties of sea water, which is used as the detection volume of the ANTARES telescope. The design, tests, construction and first results of the two types of beacons, LED and laser-based, are presented.Comment: 21 pages, 18 figures, submitted to Nucl. Instr. and Meth. Phys. Res.

Age-dependency of the prognostic impact of tumor genomics in localized resectable MYCN non-amplified neuroblastomas Report from the SIOPEN Biology Group on the LNESG Trials

BACKGROUND: Biology based treatment reduction, i.e. surgery alone also in case of not totally resected tumors, was advised in neuroblastoma patients with localized resectable disease without MYCN amplification. However, whether the genomic background of these tumors may influence outcome was unknown and therefore scrutinized in a meta-analysis comprising two prospective therapy studies and a ‘validation’ cohort. PATIENTS AND METHODS: Diagnostic samples were derived from 406 INSS stages 1/2A/2B tumors from three cohorts: LNESGI/II and COG. Genomic data were analyzed in two age groups (cut-off: 18 months) and quality controlled by the SIOPEN Biology Group. RESULTS: In both patient age groups stage 2 tumors led to similarly reduced event-free survival (5y-EFS: 83+3% versus 80+4%), but overall survival was only decreased in patients >18m (5y-OS: 97+1% versus 87+4%; p=0.001). In the latter age subgroup, only tumors with SCA led to relapses, with 11q loss as the strongest marker (5y-EFS: 40+15% versus 89+5%; p18m but not <18m. CONCLUSION: The tumor genomic make-up of resectable non-MYCN amplified stage 2 neuroblastomas has a distinct age-dependent prognostic impact in neuroblastoma patients. While in the younger age group tumors with unfavourable (SCA) and favorable genetics showed relapses, both without worsening OS, in the older age group only tumors with unfavorable genetics led to relapses and decreased OS.N/

Effect of partial soil wetting on transpiration, vegetative growth and root system of young orange trees

The wetted area fraction is a factor critical to the success of drip irrigation. This study aimed to evaluate the effect of partial soil wetting on transpiration, vegetative growth and root system of young orange trees. The experiment was carried out in a greenhouse where plants were grown in 0.5 m3boxes internally divided into compartments. The wetting of 12 % of soil area was tested on two types of soil cultivated with ‘Valencia’ orange trees grafted onto Rangpur lime and ‘Swingle’ citrumelo rootstocks. Transpiration was determined in 40 plants. Water extraction and root density were evaluated in the compartments. Transpiration is reduced by restriction in wetted soil area, and such reduction is influenced by the number of days after the beginning of partial irrigation, atmospheric evaporative demand and plant phenological stage. Mean transpiration of plants with partial irrigation was equivalent to 84 % of the mean transpiration of plants with 100 % of wetted soil area in the period studied. However, after 156 days of imposing partial irrigation there was no difference in transpiration between treatments. Plant acclimation was caused by an increase in root concentration in the irrigated area. After a period of acclimation, if the entire root system is wetted, soil water extraction becomes proportional to the percentage of wetted area after a short period of time. Despite the reduction in transpiration, there was no difference between treatments with 12 % and 100 % of wetted soil area in terms of vegetative growth

First results of the Instrumentation Line for the deep-sea ANTARES neutrino telescope

In 2005, the ANTARES Collaboration deployed and operated at a depth of 2500 m a so-called Mini Instrumentation Line equipped with Optical Modules (MILOM) at the ANTARES site. The various data acquired during the continuous operation from April to December 2005 of the MILOM confirm the satisfactory performance of the Optical Modules, their front-end electronics and readout system. as well as the calibration devices of the detector. The in situ measurement of the Optical Module time response yields a resolution better than 0.5 ns. The performance of the acoustic positioning system, which enables the spatial reconstruction of the ANTARES detector with a precision of about 10 cm, is verified. These results demonstrate that with the full ANTARES neutrino telescope the design angular resolution of better than 0.3 degrees can be realistically achieved

The data acquisition system for the ANTARES neutrino telescope

The ANTARES neutrino telescope is being constructed in the Mediterranean Sea. It consists of a large three-dimensional array of photo-multiplier tubes. The data acquisition system of the detector takes care of the digitisation of the photo-multiplier tube signals, data transport, data filtering, and data storage. The detector is operated using a control program interfaced with all elements. The design and the implementation of the data acquisition system are described.Comment: 20 pages, 6 figures, accepted for publication in Nucl. Instrum. Meth.