Plantas arom?ticas que vivifican mi ser, la yerbabuena, limoncillo, cidr?n, or?gano y manzanilla

63 P?ginasRecurso Electr?nicoLa sociedad actual frecuentemente se desvincula de los conocimientos te?rico-pr?cticos referentes al empleo de las plantas arom?ticas, olvidando los beneficios en los ?mbitos educativos, familiar y social; como objetivo central se definen estrategias pedag?gicas que favorezcan el reconocimiento y comprensi?n del beneficio con la utilizaci?n de cinco plantas arom?ticas: yerba buena, limoncillo, cidr?n, or?gano y manzanilla, en los estudiantes del CER Socorro de Sabanas del Municipio de Santa Fe de Antioquia y de la I.E. Atanasio Girardot del Municipio de Bello; con Metodolog?a de Investigaci?n Acci?n (IA) considerando los intereses del colectivo frente a la utilizaci?n y beneficios de las plantas arom?ticas, sus efectos curativos, y su empleo en emplastos, bebidas, ba?os, laxantes, entre otros usos. Se cumple con el perfil de la Licenciatura en Educaci?n B?sica establecido por la Universidad del Tolima; se aprecian hallazgos referidos a un grado de concientizaci?n respecto al empleo y uso de las plantas arom?ticas. Result? gratificante para la poblaci?n beneficiaria la adquisici?n de las nociones impartidas, la distribuci?n de macetas y el disfrute de tisanas en cada encuentro para la recolecci?n de informaci?n y socializaci?n final de los resultados. Es gratificante, como evidencia del trabajo y para afianzar los saberes tradicionales, enriquecidos con el enfoque te?rico-pr?ctico de la propuesta, la elaboraci?n y distribuci?n del M?dulo Sembramos la salud. Amerita resaltar la recuperaci?n del patrimonio generado a trav?s de los conversatorios con la comunidad adulta y los educadores de ambas instituciones educativas vinculadas.ABSTRACT. Today's society often split with the theoretical and practical knowledge regarding the use of herbs , forgetting the benefits in education , family and social spheres; like the central objective of teaching strategies that encourage the recognition and understanding of the benefit with the use of five aromatic plants: good grass, lemongrass, Cidr?n , oregano and chamomile in CER students Relief Sheets Municipality of Santa Fe de Antioquia and EI Atanasio Girardot Municipality of Bello ; Research Methodology in Action ( IA ) considering the interests of the collective over the use and benefits of herbs , their healing effects , and their use in plasters, drinks, bathrooms, laxatives, among other uses. It complies with the profile of the Bachelor of Primary Education established by the University of Tolima; findings related to a degree of awareness regarding the employment and use of aromatic plants are appreciated. Gratifying for the acquisition target population received notions, the distribution of pots and enjoys teas at each meeting for the collection of information and socialization of end results. It is gratifying, as evidence of the work and to strengthen traditional knowledge, enriched with theoretical and practical approach of the proposal, development and distribution of sow health module. Amerita highlight the recovery of assets generated through community conversations with adult educators and educational institutions both linked.INTRODUCCI?N 15 1. ANTECEDENTES DEL PROBLEMA 16 1.1. T?TULO DEL TRABAJO 16 2. JUSTIFICACI?N 19 3. FORMULACION DEL PROBLEMA 20 3.1 PREGUNTA MOVILIZADORA 20 3.2 PROBLEMA 20 4. OBJETIVOS 22 4.1 OBJETIVO GENERAL 22 4.2 OBJETIVOS ESPEC?FICOS 22 5. MARCO GENERAL 23 5.2 MARCO CONTEXTUAL 24 5.2.1 Contexto comunitario 24 5.3 COMPONENTE PEDAG?GICO 27 5.3.1 Categor?as generales cultura 27 5.4 COMPONENTE ESPEC?FICO DE ESTUDIO 29 5.4.1 Categor?as espec?ficas 29 5.4.2 Plantas arom?ticas 29 5.4.3 Formas de uso 31 5.4.4 Beneficios 32 5.5 CONSOLIDADO DE LAS PLANTAS OBJETO DE ESTUDIO 32 6. DISE?O METODOL?GICO DEL PROCESO INVESTIGATIVO 40 6.1 POBLACI?N BENEFICIARIA 40 6.2 METODOLOG?A DE TRABAJO 41 6.3 UNIVERSO Y MUESTRA 41 6.4 TIPO DE ESTUDIO 41 6.5 ?REA DE ESTUDIO 42 6.6 PROCEDIMIENTOS DEL DISE?O METODOL?GICO 43 6.7 ESTRATEGIAS DE TRABAJO 43 6.8 INSTRUMENTOS PARA LA RECOLECCI?N DE LA INFORMACI?N 44 7. AN?LISIS DE LA INFORMACI?N 45 7.1 ACTIVIDADES POR DESARROLLAR 45 7.1.1 Etapa inicial 45 7.1.2 Etapa de ejecuci?n 46 7.1.3 Etapa de finalizaci?n 46 8. CRONOGRAMA 47 9. PRESUPUESTO GLOBAL DE LA PROPUESTA 50 10. RECURSOS 53 11. CONCLUSIONES 54 RECOMENDACIONES 55 REFERENCIAS 5

Role of Ovarian Proteins Secreted by Toxoneuron nigriceps (Viereck) (Hymenoptera, Braconidae) in the Early Suppression of Host Immune Response

Toxoneuron nigriceps (Viereck) (Hymenoptera, Braconidae) is an endophagous parasitoid of the larval stages of the tobacco budworm, Heliothis virescens (Fabricius) (Lepidoptera, Noctuidae). During oviposition, T. nigriceps injects into the host body, along with the egg, the venom, the calyx fluid, which contains a Polydnavirus (T. nigriceps BracoVirus: TnBV), and the Ovarian Proteins (OPs). Although viral gene expression in the host reaches detectable levels after a few hours, a precocious disruption of the host metabolism and immune system is observed right after parasitization. This alteration appears to be induced by female secretions including TnBV venom and OPs. OPs, originating from the ovarian calyx cells, are involved in the induction of precocious symptoms in the host immune system alteration. It is known that OPs in braconid and ichneumonid wasps can interfere with the cellular immune response before Polydnavirus infects and expresses its genes in the host tissues. Here we show that T. nigriceps OPs induce several alterations on host haemocytes that trigger cell death. The OP injection induces an extensive oxidative stress and a disorganization of actin cytoskeleton and these alterations can explain the high-level of haemocyte mortality, the loss of haemocyte functionality, and so the reduction in encapsulation ability by the host

TARTESSUS: A customized electrospun drug delivery system loaded with Irinotecan for Local and sustained chemotherapy release in pancreatic cancer

Post-surgical chemotherapy in pancreatic cancer has notorious side effects due to the high dose required. Multiple devices have been designed to tackle this aspect and achieve a delayed drug release. This study aimed to explore the controlled and sustained local delivery of a reduced drug dose from an irinotecan-loaded electrospun nanofiber membrane (named TARTESSUS) that can be placed on the patients' tissue after tumor resection surgery. The drug delivery system formulation was made of polycaprolactone (PCL). The mechanical properties and the release kinetics of the drug were adjusted by the electrospinning parameters and by the polymer ratio between 10 w.t.% and 14 w.t.% of PCL in formic acid:acetic acid:chloroform (47.5:47.5:5). The irinotecan release analysis was performed and three different release periods were obtained, depending on the concentration of the polymer in the dissolution. The TARTESSUS device was tested in 2D and 3D cell cultures and it demonstrated a decrease in cell viability in 2D culture between 72 h and day 7 from the start of treatment. In 3D culture, a decrease in viability was seen between 72 h, day 7 (p < 0.001), day 10 (p < 0.001), 14 (p < 0.001), and day 17 (p = 0.003) as well as a decrease in proliferation between 72 h and day 10 (p = 0.030) and a reduction in spheroid size during days 10 (p = 0.001), 14 (p < 0.001), and 17 (p < 0.001). In conclusion, TARTESSUS showed a successful encapsulation of a chemotherapeutic drug and a sustained and delayed release with an adjustable releasing period to optimize the therapeutic effect in pancreatic cancer treatment

Development of nanomechanical biosensors for the determination of cell mechanical properties

Póster presentado en el GEM4 Summer school, celebrado en Londres del 10 al 14 de septiembre de 2012.Peer Reviewe

Screening for cervical Cancer in high-risk populations: DNA pap test or hybrid capture II test alone?

This study was designed to evaluate whether Hybrid Capture II (HC2) test alone refer women to colposcopy as appropriately as DNA Papanicolaou (Pap) test, in the context of a high-risk group of women using the recently validated DNACitoliq LBC system. Women with suspected cervical disease were included in this crosssectional study at a tertiary center in São Paulo, Brazil, for further workup. All women had cervical material collected for LBC and HC2 for high-risk human papillomavirus (hrHPV)-DNA test. Irrespective of cytology and HC2 results, colposcopy, and cervical biopsy when applicable, was systematically performed. All tests were performed blindly. Sensitivity, specificity, positive and negative predictive values, and overall accuracy of both methods were computed in relation to histology. A total of 1,080 women were included: 36.4% (393/1080) had ACUS+, 10.2% (110/1080) were high-grade squamous intraepithelial lesions (HSIL) or cancer. Mean age was 33.5 years. All women underwent colposcopy, and cervical biopsies were performed in 38.4% (415/1080): 33% (137/415) of the biopsies were negative, 14.4% (155/415) were low-grade squamous intraepithelial lesions (LSIL), 10.7% (116/415) were HSIL, and 0.6% (7/415) were cancer. HC2 sensitivity to diagnose biopsy-proven HSIL was 100%. Because all HSIL cases had a positive HC2 test, sensitivity could not be improved by adding LBC. Specificity and positive and negative predictive values of DNA Pap were not significantly different from HC2 test alone when considering LSIL+ histology as ‘‘gold standard’’ and HSIL+ histology. As a screening strategy for women with high-risk for cervical cancer, DNA Pap test does not seem to add substantially to HC2 alone in terms of appropriately referring to colposcopy

Biopsy confirmation of metastatic sites in breast cancer patients:clinical impact and future perspectives

Determination of hormone receptor (estrogen receptor and progesterone receptor) and human epidermal growth factor receptor 2 status in the primary tumor is clinically relevant to define breast cancer subtypes, clinical outcome,and the choice of therapy. Retrospective and prospective studies suggest that there is substantial discordance in receptor status between primary and recurrent breast cancer. Despite this evidence and current recommendations,the acquisition of tissue from metastatic deposits is not routine practice. As a consequence, therapeutic decisions for treatment in the metastatic setting are based on the features of the primary tumor. Reasons for this attitude include the invasiveness of the procedure and the unreliable outcome of biopsy, in particular for biopsies of lesions at complex visceral sites. Improvements in interventional radiology techniques mean that most metastatic sites are now accessible by minimally invasive methods, including surgery. In our opinion, since biopsies are diagnostic and changes in biological features between the primary and secondary tumors can occur, the routine biopsy of metastatic disease needs to be performed. In this review, we discuss the rationale for biopsy of suspected breast cancer metastases, review issues and caveats surrounding discordance of biomarker status between primary and metastatic tumors, and provide insights for deciding when to perform biopsy of suspected metastases and which one (s) to biopsy. We also speculate on the future translational implications for biopsy of suspected metastatic lesions in the context of clinical trials and the establishment of bio-banks of biopsy material taken from metastatic sites. We believe that such bio-banks will be important for exploring mechanisms of metastasis. In the future,advances in targeted therapy will depend on the availability of metastatic tissue

Cognitive impairment induced by delta9-tetrahydrocannabinol occurs through heteromers between cannabinoid CB1 and serotonin 5-HT2A receptors

Delta-9-tetrahydrocannabinol (THC), the main psychoactive compound of marijuana, induces numerous undesirable effects, including memory impairments, anxiety, and dependence. Conversely, THC also has potentially therapeutic effects, including analgesia, muscle relaxation, and neuroprotection. However, the mechanisms that dissociate these responses are still not known. Using mice lacking the serotonin receptor 5-HT2A, we revealed that the analgesic and amnesic effects of THC are independent of each other: while amnesia induced by THC disappears in the mutant mice, THC can still promote analgesia in these animals. In subsequent molecular studies, we showed that in specific brain regions involved in memory formation, the receptors for THC and the 5-HT2A receptors work together by physically interacting with each other. Experimentally interfering with this interaction prevented the memory deficits induced by THC, but not its analgesic properties. Our results highlight a novel mechanism by which the beneficial analgesic properties of THC can be dissociated from its cognitive side effects

The Interplay between Entamoeba and Enteropathogenic Bacteria Modulates Epithelial Cell Damage

In amoebiasis, a human disease that is a serious health problem in many developing countries, efforts have been made to identify responsible factors for the tissue damage inflicted by the parasite Entamoeba histolytica. This amoeba lives in the lumen of the colon without causing damage to the intestinal mucosa, but under unknown circumstances becomes invasive, destroying the intestinal tissue. Bacteria in the intestinal flora have been proposed as inducers of higher amoebic virulence, but the causes or mechanisms responsible for the induction are still undetermined. Mixed intestinal infections with Entamoeba histolytica and enteropathogenic bacteria, showing exacerbated manifestations of disease, are common in endemic countries. We implemented an experimental system to study amoebic virulence in the presence of pathogenic bacteria and its consequences on epithelial cells. Results showed that amoebae that ingested enteropathogenic bacteria became more virulent, causing more damage to epithelial cells. Bacteria induced release of inflammatory proteins by the epithelial cells that attracted amoebae, facilitating amoebic contact to the epithelial cells and higher damage. Our results, although a first approach to this complex problem, provide insights into amoebic infections, as interplay with other pathogens apparently influences the intestinal environment, the behavior of cells involved and the manifestations of the disease