Purification, Characterization, and Effect of Thiol Compounds on Activity of the Erwinia carotovora L-Asparaginase

L-asparaginase was extracted from Erwinia carotovora and purified by ammonium sulfate fractionation (60–70%), Sephadex G-100, CM cellulose, and DEAE sephadex chromatography. The apparent Mr of enzyme under nondenaturing and denaturing conditions was 150 kDa and 37 ± 0.5 kDa, respectively. L-asparaginase activity was studied in presence of thiols, namely, L-cystine (Cys), L-methionine (Met), N-acetyl cysteine (NAC), and reduced glutathione (GSH). Kinetic parameters in presence of thiols (10–400 μM) showed an increase in Vmax values (2000, 2223, 2380, 2500, and control 1666.7 μmoles mg−1min−1) and a decrease in Km values (0.086, 0.076, 0.062, 0.055 and control 0.098 mM) indicating nonessential mode of activation. KA values displayed propensity to bind thiols. A decrease in Vmax/Km ratio in concentration plots showed inverse relationship between free thiol groups (NAC and GSH) and bound thiol group (Cys and Met). Enzyme activity was enhanced in presence of thiol protecting reagents like dithiothreitol (DTT), 2-mercaptoethanol (2-ME), and GSH, but inhibited by p-chloromercurybenzoate (PCMB) and iodoacetamide (IA)

Fair Use Provisions under the Indian Copyright Act: Awareness Among Librarians of Colleges and Universities in Maharashtra

7-15Indian Copyright Act, 1957 provides a private right in copyright which extensively deals with provisions that guarantee access to knowledge as well. Copyright is an exclusive and assignable legal right given to the author for a particular period for original literary, dramatic, artistic works, and musical work whether published or unpublished. These rights are not absolute but subject to restrictions and limitations imposed by law. Section 52 of the Copyright Act, 1957 is one such provision that restraints the exclusive enjoyment of rights given to the copyright holder. As per Section 52 (1)(o) of the Copyright Act, 1957 a non-commercial library is permitted/allowed to make three copies of a book for library use, provided such books are not available for sale in India. The present article is about the concept of fair dealing which is originated from the Common of laws of England. Although, the concept of fair use and fair dealing are used synonymously, the Indian Copyright Act, 1957 deals with fair dealing under Section 52 of the Copyright Act, 1957. The reproductions of works for research or private study, taking photocopy for research and educational purpose, etc., are covered under the doctrine of fair use. This paper will explain the conceptual framework of the Indian Copyright Act and the empirical reality in libraries of Maharashtra. It attempts to empirically test the usage of fair use provisions under the Indian Copyright Act and the awareness among librarians of Colleges and Universities in Maharashtra. The research methodology used for the present study is both doctrinal and non-doctrinal. It was observed that most of the librarians are aware of Copyright Law and copyright exceptions but when it comes to the implementation librarians also face difficulty and it leads to the infringement of copyright

EXPERIMENTAL PERFORMANCE OF DIFFERENT EVAPORATIVE COOLING PAD MATERIAL OF DIRECT EVAPORATIVE COOLER IN HOT AND DRY REGION

An experimental performance of evaporative cooling pads of different materials based on weather data of vidharbha, India has been carried out. Saturation efficiency and cooling capacity of thickness 4 inch cooling pad materials were measured. Effect of air and water flow rate on saturation efficiency and cooling capacity has been investigated for different cooling pad materials like cellulose, khus-grass, and wood-wool material. Saturation efficiency and cooling capacity have been calculated for flow rates of air between 0.25 to 0.45 m3/s and for water flow rate of 60 to 100 cc/hr. Saturation efficiency and cooling capacity variation with water and air flow rate is plotted for different materials of the pads. It has been observed that cellulose material gives highest saturation efficiency of about 92.8% while Khus-Grass material gives lowest saturation efficiency of about 40.13%. The cooling capacity increases with air flow rate and is obtained between 1.1 to 6.72 kW for different materials

Effect of maturity stages on the quality indices of wood apple (Feronia limonia) and modeling of its kinetics by applying machine learning approaches

In the present investigation, an inexpensive and non-destructive method was tested for the appropriate maturity classification of wood apple (Feronia limonia). The investigation was conducted to establish the pronounced effect of maturity stages on the growth kinetics, physico-chemical properties, and other quality indices of wood apple. A systematic trend was observed for all the properties namely sphericity, bulk density (g/cm3), true density (g/cm3), pH, total soluble solids TSS (°Brix), titratable acidity (%) and TSS/TA ratio, etc. of the fruit. In contrast, regular changes were also observed in the color properties at various maturity stages of the wood apple. The maturity kinetics was formulated by applying recurrent neural network (RNN) in compliance with K means cluster algorithm. RNN modeling was applied by considering color property (redness value) as input and six maturity indices as the output of the formulated structure. The RNN architecture, 1-6-6 showed the best results for forecasting the wood apple maturity based on color features. Further, based on the results of the K means cluster algorithm, the maturity stages were classified into three main categories, illustrated in the form of a simplified color chart. Hence, this investigation can be useful for proper control and identification of wood apple maturity during the processing

MOTION ANALYSIS IN VIDEO USING OPTICAL FLOW TECHNIQUES

ABSTRACT: This paper presents optical flow estimation technique to estimate the motion vectors in each frame of the video sequence. By thresholding and performing morphological closing on the motion vectors, we produces binary feature images. Using these binary features the cars are located. A bounding Box is drawn around the cars that pass beneath the white line. The algorithm used for this is lucas kanade. Use of the threshold to reduce the noise in small movements between frames is analyzed. Higher the threshold ,the less small movements impact the optical flow calculation. Experiments are done to find the value that best achieves our results

Poboljšan postupak sinteze nekih novih 1,3-diaril-2-propen-1-ona koristeći PEG-400 kao reciklirajuće otapalo i njihovo antimikrobno vrednovanje

A simple and convenient route is described for the synthesis of novel hetero 1,3-diaryl-2-propen-1-ones (chalcones) by using recyclable poly PEG-400 as an alternative reaction solvent. The reaction is clean with excellent yield, shorter reaction time and reduces the use of volatile organic compounds (VOCs). All the synthesized compounds were evaluated for their antimicrobial activities against several pathogenic representatives.Opisana je jednostavna i pogodna metoda sinteze novih hetero 1,3-diaril-2-propen-1-ona (kalkona) koristeći poli(etilenglikol) (PEG-400) kao alternativno otapalo. Reakcija je jednoznačna, a uporaba hlapljivih organskih otapala je smanjena. Iskorištenja na produktima su visoka, a reakcijska vremena kraća. Svi sintetizirani spojevi testirani su na antimikrobno djelovanje na nekoliko patogenih mikroorganizama

Characterization and physical properties of aluminium foam−polydimethylsiloxane nanocomposite hybrid structures

This article reports on the fabrication and characterisation of hybrid structures prepared by impregnating an open-cell aluminum foam with polydimethylsiloxane (PDMS) or PDMS reinforced with graphene oxide, GO (PDMS nanocomposite). The effect of the PDMS and the GO on the mechanical, thermal, acoustic absorption and fire retardancy properties of the resulting hybrid structures were evaluated and compared to the individual components (PDMS, PDMS nanocomposite, open-cell aluminium foams). Results demonstrate that the use of the PDMS cured at 65 °C, as an void filler of the open-cell aluminium foams, changes mechanical and deformation performance, from a rubbery to brittle behaviour, however attaining a higher level of strength (quasi-static: ∼5 MPa; dynamic: > 15 MPa) in the resulting hybrid structures. This change is due to the low chain mobility of the polymer and effective adhesion with struts of the open-cell aluminium foams. Furthermore, these hybrid structures are extremely sensitive to strain-rate testing, exhibiting a maximum compressive stress increase of more than 300 % and 200 %, respectively. The presence of the GO within the PDMS improves significantly the non-flammability of the hybrid structures and increases the sound absorption coefficient.publishe

A workshop on ‘Dietary Sweetness—Is It an Issue?’

This report summarises a workshop convened by ILSI Europe on 3 and 4 April 2017 to discuss the issue of dietary sweetness. The objectives were to understand the roles of sweetness in the diet, establish whether exposure to sweetness affects diet quality and energy intake, and consider whether sweetness per se affects health. Although there may be evidence for tracking of intake of some sweet components of the diet through childhood, evidence for tracking of whole diet sweetness, or through other stages of maturity are lacking. The evidence to date does not support adverse effects of sweetness on diet quality or energy intake, except where sweet food choices increase intake of free sugars. There is some evidence for improvements in diet quality and reduced energy intake where sweetness without calories replaces sweetness with calories. There is a need to understand the physiological and metabolic relevance of sweet taste receptors on the tongue, in the gut and elsewhere in the body, as well as possible differentiation in the effects of sustained consumption of individual sweeteners. Despite a plethora of studies, there is no consistent evidence for an association of sweetness sensitivity/preference with obesity or type 2 diabetes. A multifaceted integrated approach, characterising nutritive and sensory aspects of the whole diet or dietary patterns, may be more valuable in providing contextual insight. The outcomes of the workshop could be used as a scientific basis to inform the expert community and create more useful dialogue among health care professionals

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries