1,440 research outputs found
On the Calibration of Full-polarization 86GHz Global VLBI Observations
We report the development of a semi-automatic pipeline for the calibration of
86 GHz full-polarization observations performed with the Global Millimeter-VLBI
array (GMVA) and describe the calibration strategy followed in the data
reduction. Our calibration pipeline involves non-standard procedures, since
VLBI polarimetry at frequencies above 43 GHz is not yet well established. We
also present, for the first time, a full-polarization global-VLBI image at 86
GHz (source 3C 345), as an example of the final product of our calibration
pipeline, and discuss the effect of instrumental limitations on the fidelity of
the polarization images. Our calibration strategy is not exclusive for the
GMVA, and could be applied on other VLBI arrays at millimeter wavelengths. The
use of this pipeline will allow GMVA observers to get fully-calibrated datasets
shortly after the data correlation.Comment: 10 pages, 10 figures. Accepted for publication in A&
Identification of genomic biomarkers for anthracycline-induced cardiotoxicity in human iPSC-derived cardiomyocytes: an in vitro repeated exposure toxicity approach for safety assessment.
The currently available techniques for the safety evaluation of candidate drugs are usually cost-intensive and time-consuming and are often insufficient to predict human relevant cardiotoxicity. The purpose of this study was to develop an in vitro repeated exposure toxicity methodology allowing the identification of predictive genomics biomarkers of functional relevance for drug-induced cardiotoxicity in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). The hiPSC-CMs were incubated with 156 nM doxorubicin, which is a well-characterized cardiotoxicant, for 2 or 6 days followed by washout of the test compound and further incubation in compound-free culture medium until day 14 after the onset of exposure. An xCELLigence Real-Time Cell Analyser was used to monitor doxorubicin-induced cytotoxicity while also monitoring functional alterations of cardiomyocytes by counting of the beating frequency of cardiomyocytes. Unlike single exposure, repeated doxorubicin exposure resulted in long-term arrhythmic beating in hiPSC-CMs accompanied by significant cytotoxicity. Global gene expression changes were studied using microarrays and bioinformatics tools. Analysis of the transcriptomic data revealed early expression signatures of genes involved in formation of sarcomeric structures, regulation of ion homeostasis and induction of apoptosis. Eighty-four significantly deregulated genes related to cardiac functions, stress and apoptosis were validated using real-time PCR. The expression of the 84 genes was further studied by real-time PCR in hiPSC-CMs incubated with daunorubicin and mitoxantrone, further anthracycline family members that are also known to induce cardiotoxicity. A panel of 35 genes was deregulated by all three anthracycline family members and can therefore be expected to predict the cardiotoxicity of compounds acting by similar mechanisms as doxorubicin, daunorubicin or mitoxantrone. The identified gene panel can be applied in the safety assessment of novel drug candidates as well as available therapeutics to identify compounds that may cause cardiotoxicity
The Bright Gamma-Ray Burst 991208 - Tight Constraints on Afterglow Models from Observations of the Early-Time Radio Evolution
The millimeter wavelength emission from GRB 991208 is the second brightest
ever detected, yielding a unique data set. We present here well-sampled spectra
and light curves over more than two decades in frequency for a two-week period.
This data set has allowed us for the first time to trace the evolution of the
characteristic synchrotron self-absorption frequency nu_a and peak frequency
nu_m, and the peak flux density F_m: we obtain nu_a \propto t^{-0.15 +- 0.12},
nu_m \propto t^{-1.7 +- 0.4}, and $_m \propto t^{-0.47 +- 0.11}. From the radio
data we find that models of homogeneous or wind-generated ambient media with a
spherically symmetric outflow can be ruled out. A model in which the
relativistic outflow is collimated (a jet) can account for the observed
evolution of the synchrotron parameters, the rapid decay at optical
wavelengths, and the observed radio to optical spectral flux distributions that
we present here, provided that the jet transition has not been fully completed
in the first two weeks after the event. These observations provide additional
evidence that rapidly decaying optical/X-ray afterglows are due to jets and
that such transitions either develop very slowly or perhaps never reach the
predicted asymptotic decay F(t) \propto t^{-p}.Comment: 15 pages, 4 figures, submitted to ApJ Letter
Star formation in the early universe: beyond the tip of the iceberg
We present late-time Hubble Space Telescope imaging of the fields of six
Swift GRBs lying at 5.0<z<9.5. Our data includes very deep observations of the
field of the most distant spectroscopically confirmed burst, GRB 090423, at
z=8.2. Using the precise positions afforded by their afterglows we can place
stringent limits on the luminosities of their host galaxies. In one case, that
of GRB 060522 at z=5.11, there is a marginal excess of flux close to the GRB
position which may be a detection of a host at a magnitude J(AB)=28.5. None of
the others are significantly detected meaning that all the hosts lie below
L\star at their respective redshifts, with star formation rates SFR<4Mo/yr in
all cases. Indeed, stacking the five fields with WFC3-IR data we conclude a
mean SFR<0.17Mo/yr per galaxy. These results support the proposition that the
bulk of star formation, and hence integrated UV luminosity, at high redshifts
arises in galaxies below the detection limits of deep-field observations.
Making the reasonable assumption that GRB rate is proportional to UV luminosity
at early times allows us to compare our limits with expectations based on
galaxy luminosity functions derived from the Hubble Ultra-Deep Field (HUDF) and
other deep fields. We infer that a luminosity function which is evolving
rapidly towards steeper faint-end slope (alpha) and decreasing characteristic
luminosity (L\star), as suggested by some other studies, is consistent with our
observations, whereas a non-evolving LF shape is ruled out at >90% confidence.
Although it is not yet possible to make stronger statements, in the future,
with larger samples and a fuller understanding of the conditions required for
GRB production, studies like this hold great potential for probing the nature
of star formation, the shape of the galaxy luminosity function, and the supply
of ionizing photons in the early universe.Comment: ApJ in press. 14 pages, 6 figures. (small updates from version 1
Cerebrospinal fluid findings in patients with neurological manifestations in post-COVID-19 syndrome
BACKGROUND: Information on cerebrospinal fluid (CSF) findings in patients with neurological manifestations in post-COVID-19 syndrome is scarce. METHODS: Retrospective evaluation of 84 CSF samples in patients fulfilling post-COVID-19 criteria in two neurological post-COVID-19 outpatient clinics. RESULTS: In 68% of samples, all CSF parameters were normal. The most frequent pathological CSF finding was elevation of total protein (median total protein 33.3 mg/dl [total range 18.5-116.2]) in 20 of 83 (24%) samples. The second most prevalent pathological finding was a blood-CSF barrier dysfunction as measured by elevation of QAlb (median QAlb 4.65 [2.4-13.2]) in 11/84 (13%). Pleocytosis was found in only 5/84 (6%) samples and was mild in all of them. CSF-restricted oligoclonal bands were found in 5/83 (6%) samples. Anti-neuronal autoantibodies in CSF were negative in most cases, whilst 12/68 (18%) samples were positive for anti-myelin autoantibodies in serum. PCR for herpesviridae (HSV-1/-2, VZV, EBV, CMV, HHV6) showed, if at all, only weakly positive results in CSF or EDTA whole blood/plasma. CONCLUSIONS: The majority of samples did not show any pathologies. The most frequent findings were elevation of total protein and blood-CSF barrier dysfunction with no signs of intrathecal inflammation. CSF analysis still keeps its value for exclusion of differential diagnoses
Complete chloroplast genome sequence of Holoparasite Cistanche Deserticola (Orobanchaceae) reveals gene loss and horizontal gene transfer from Its host Haloxylon Ammodendron (Chenopodiaceae)
The central function of chloroplasts is to carry out photosynthesis, and its gene content and structure are highly conserved across land plants. Parasitic plants, which have reduced photosynthetic ability, suffer gene losses from the chloroplast (cp) genome accompanied by the relaxation of selective constraints. Compared with the rapid rise in the number of cp genome sequences of photosynthetic organisms, there are limited data sets from parasitic plants. The authors report the complete sequence of the cp genome of Cistanche deserticola, a holoparasitic desert species belonging to the family Orobanchaceae
Challenging GRB models through the broadband dataset of GRB060908
Context: Multiwavelength observations of gamma-ray burst prompt and afterglow
emission are a key tool to disentangle the various possible emission processes
and scenarios proposed to interpret the complex gamma-ray burst phenomenology.
Aims: We collected a large dataset on GRB060908 in order to carry out a
comprehensive analysis of the prompt emission as well as the early and late
afterglow. Methods: Data from Swift-BAT, -XRT and -UVOT together with data from
a number of different ground-based optical/NIR and millimeter telescopes
allowed us to follow the afterglow evolution from about a minute from the
high-energy event down to the host galaxy limit. We discuss the physical
parameters required to model these emissions. Results: The prompt emission of
GRB060908 was characterized by two main periods of activity, spaced by a few
seconds of low intensity, with a tight correlation between activity and
spectral hardness. Observations of the afterglow began less than one minute
after the high-energy event, when it was already in a decaying phase, and it
was characterized by a rather flat optical/NIR spectrum which can be
interpreted as due to a hard energy-distribution of the emitting electrons. On
the other hand, the X-ray spectrum of the afterglow could be fit by a rather
soft electron distribution. Conclusions: GRB060908 is a good example of a
gamma-ray burst with a rich multi-wavelength set of observations. The
availability of this dataset, built thanks to the joint efforts of many
different teams, allowed us to carry out stringent tests for various
interpretative scenarios showing that a satisfactorily modeling of this event
is challenging. In the future, similar efforts will enable us to obtain
optical/NIR coverage comparable in quality and quantity to the X-ray data for
more events, therefore opening new avenues to progress gamma-ray burst
research.Comment: A&A, in press. 11 pages, 5 figure
The extraordinarily bright optical afterglow of GRB 991208 and its host galaxy
Observations of the extraordinarily bright optical afterglow (OA) of GRB
991208 started 2.1 d after the event. The flux decay constant of the OA in the
R-band is -2.30 +/- 0.07 up to 5 d, which is very likely due to the jet effect,
and after that it is followed by a much steeper decay with constant -3.2 +/-
0.2, the fastest one ever seen in a GRB OA. A negative detection in several
all-sky films taken simultaneously to the event implies either a previous
additional break prior to 2 d after the occurrence of the GRB (as expected from
the jet effect). The existence of a second break might indicate a steepening in
the electron spectrum or the superposition of two events. Once the afterglow
emission vanished, contribution of a bright underlying SN is found, but the
light curve is not sufficiently well sampled to rule out a dust echo
explanation. Our determination of z = 0.706 indicates that GRB 991208 is at 3.7
Gpc, implying an isotropic energy release of 1.15 x 10E53 erg which may be
relaxed by beaming by a factor > 100. Precise astrometry indicates that the GRB
coincides within 0.2" with the host galaxy, thus given support to a massive
star origin. The absolute magnitude is M_B = -18.2, well below the knee of the
galaxy luminosity function and we derive a star-forming rate of 11.5 +/- 7.1
Mo/yr. The quasi-simultaneous broad-band photometric spectral energy
distribution of the afterglow is determined 3.5 day after the burst (Dec 12.0)
implying a cooling frequency below the optical band, i.e. supporting a jet
model with p = -2.30 as the index of the power-law electron distribution.Comment: Accepted for publication in Astronomy and Astrophysics, 9 pages, 6
figures (Fig. 3 and Fig. 4 have been updated
A new measurement of direct CP violation in two pion decays of the neutral kaon
The NA48 experiment at CERN has performed a new measurement of direct CP
violation, based on data taken in 1997 by simultaneously collecting K_L and K_S
decays into pi0pi0 and pi+pi-. The result for the CP violating parameter
Re(epsilon'/epsilon) is (18.5 +/- 4.5(stat)} +/- 5.8 (syst))x10^{-4}.Comment: 18 pages, 6 figure
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