Design, Construction and In Situ Testing of a Muon Camera for Earth Science and Civil Engineering Applications

The MUST2 (MUon Survey Tomography based on Micromegas detectors for Unreachable Sites Technology) camera is based on a thin Time Projection Chamber read by a resistive Micromegas. This innovative combination presents interesting distinctive features compared to existing muon detection technologies. It allows a wide angular acceptance of the detector with a low weight and compact volume, well adapted for confined spaces or underground operation. The current work presents the results obtained during the calibration measurements at the reference site, the Low Background Noise Laboratory (LBNL). Preliminary results from field measurement campaign carried out at the dam overlooking the village of Saint-Saturnin-les-Apt (South-East of France) are presented and discussed

Development and applications of micro-pattern gaseous detectors for muon tomography

Cette thèse décrit les premiers essais de tomographie muonique par absorption et par déviation en utilisant des détecteurs Micromegas à haute granularité. Cette technique d'imagerie utilisant les rayons cosmiques gratuits, sans dangers et disponibles partout a démontré sa capacité à imager des objets de tailles variées. Afin de construire des outils compacts, précis, et portables, utiliser une voie d'électronique pour lire chaque motif de lecture est impossible. Pour éviter ce problème, des détecteurs multiplexés ont été conçus, testés et mis en situation dans différentes conditions. Il a été tiré parti des dernières améliorations concernant le détecteur Micromegas telles que le multiplexage génétique ou la lecture 2D par pistes sous une couche résistive. Les prototypes qui ont été fabriqués ont atteint une résolution de 300µm sur une surface d'un quart de mètre carré en ne nécessitant que 61 voies d'électronique. Grâce à ces détecteurs, des campagnes de prise de données ont été faites, à la fois dans l'environnement semi-contrôlé du centre CEA de Saclay et sur le plateau de Gizeh en Egypte. Ces deux campagnes ont permis d'imager avec succès le château d'eau du CEA Saclay ainsi que la pyramide de Khéops et ce malgré les conditions extrêmes que les télescopes à muon ont endurées. Des variations de température de plusieurs dizaines de Kelvin ont été enregistrées alors que l'acquisition de données se déroulait de manière stable, c'est-à-dire que les variations du gain n'impactaient pas le système d'auto déclenchement. Cette stabilité a été rendue possible grâce à un ajustement des hautes tensions vis à vis des conditions environnementales. Cela constitue la première mondiale concernant le fonctionnement d'un dispositif de reconstruction de trace à base de Micromégas en extérieur. En parallèle des expériences de muographie par déviation ont été menées. Un dispositif imageant des objets de petite taille est capable de distinguer divers matériaux sur une échelle de temps de l'ordre d'une journée. Une plus grande installation a permis d'imager un conteneur entier. La résolution du problème inverse a été faite en utilisant à la fois l'algorithme simple dit du PoCA ainsi que celui de maximisation de vraisemblance proposé par Schultz et son équipe.This thesis describes the first attempts to perform both absorption and scattering muon tomography using high granularity Micromegas detectors. This imaging technique using the free, available and harmless cosmic ray muons radiation shows great possibilities to study various sized objects. In order to make compact and precise portable devices, using one channel of electronics per readout pattern is not possible. To avoid this problem multiplexed detectors have been designed, extensively tested and used in numerous conditions. Latest developments in Micromegas design have been used such as the genetic multiplexing and the 2D strip readout using a resistive layer. The prototypes made were able to achieve a 300µm resolution at the scale of 50cm while using only 61 channels of electronics. Using these detectors, muography data taking campaigns have been performed both in the semi-controlled environment of the Saclay site of CEA and in the wild of the Giza plateau in Egypt. These two campaigns succeeded in imaging the CEA Saclay water tower and the Khufu's pyramid despite the extreme conditions endured by the Micromegas muon telescopes. Large temperature variations of a few tens of Kelvin have been recorded together with a stable operation i.e. an even gain ensuring a steady self triggering system. This stability was achieved using high voltage variations with respect to the environmental conditions. Together with this very first worldwide operation of a Micromegas-based tracker outside a laboratory, scattering muographies have also been done. A small setup imaging handheld objects performed well in separating various materials in time scales of the order of the day while a bigger 1m² setup allowing the scan of a full container was successfully operated. The inversion of the ill-posed problem of the muon scattering was performed using the crude PoCA method and the maximum likelihood one described by Schultz et al

Développement et applications de détecteurs gazeux à micro-pistes pour la tomographie muonique

This thesis describes the first attempts to perform both absorption and scattering muon tomography using high granularity Micromegas detectors. This imaging technique using the free, available and harmless cosmic ray muons radiation shows great possibilities to study various sized objects. In order to make compact and precise portable devices, using one channel of electronics per readout pattern is not possible. To avoid this problem multiplexed detectors have been designed, extensively tested and used in numerous conditions. Latest developments in Micromegas design have been used such as the genetic multiplexing and the 2D strip readout using a resistive layer. The prototypes made were able to achieve a 300µm resolution at the scale of 50cm while using only 61 channels of electronics. Using these detectors, muography data taking campaigns have been performed both in the semi-controlled environment of the Saclay site of CEA and in the wild of the Giza plateau in Egypt. These two campaigns succeeded in imaging the CEA Saclay water tower and the Khufu's pyramid despite the extreme conditions endured by the Micromegas muon telescopes. Large temperature variations of a few tens of Kelvin have been recorded together with a stable operation i.e. an even gain ensuring a steady self triggering system. This stability was achieved using high voltage variations with respect to the environmental conditions. Together with this very first worldwide operation of a Micromegas-based tracker outside a laboratory, scattering muographies have also been done. A small setup imaging handheld objects performed well in separating various materials in time scales of the order of the day while a bigger 1m² setup allowing the scan of a full container was successfully operated. The inversion of the ill-posed problem of the muon scattering was performed using the crude PoCA method and the maximum likelihood one described by Schultz et al.Cette thèse décrit les premiers essais de tomographie muonique par absorption et par déviation en utilisant des détecteurs Micromegas à haute granularité. Cette technique d'imagerie utilisant les rayons cosmiques gratuits, sans dangers et disponibles partout a démontré sa capacité à imager des objets de tailles variées. Afin de construire des outils compacts, précis, et portables, utiliser une voie d'électronique pour lire chaque motif de lecture est impossible. Pour éviter ce problème, des détecteurs multiplexés ont été conçus, testés et mis en situation dans différentes conditions. Il a été tiré parti des dernières améliorations concernant le détecteur Micromegas telles que le multiplexage génétique ou la lecture 2D par pistes sous une couche résistive. Les prototypes qui ont été fabriqués ont atteint une résolution de 300µm sur une surface d'un quart de mètre carré en ne nécessitant que 61 voies d'électronique. Grâce à ces détecteurs, des campagnes de prise de données ont été faites, à la fois dans l'environnement semi-contrôlé du centre CEA de Saclay et sur le plateau de Gizeh en Egypte. Ces deux campagnes ont permis d'imager avec succès le château d'eau du CEA Saclay ainsi que la pyramide de Khéops et ce malgré les conditions extrêmes que les télescopes à muon ont endurées. Des variations de température de plusieurs dizaines de Kelvin ont été enregistrées alors que l'acquisition de données se déroulait de manière stable, c'est-à-dire que les variations du gain n'impactaient pas le système d'auto déclenchement. Cette stabilité a été rendue possible grâce à un ajustement des hautes tensions vis à vis des conditions environnementales. Cela constitue la première mondiale concernant le fonctionnement d'un dispositif de reconstruction de trace à base de Micromégas en extérieur. En parallèle des expériences de muographie par déviation ont été menées. Un dispositif imageant des objets de petite taille est capable de distinguer divers matériaux sur une échelle de temps de l'ordre d'une journée. Une plus grande installation a permis d'imager un conteneur entier. La résolution du problème inverse a été faite en utilisant à la fois l'algorithme simple dit du PoCA ainsi que celui de maximisation de vraisemblance proposé par Schultz et son équipe

Phages rarely encode antibiotic resistance genes: a cautionary tale for virome analyses.

Antibiotic resistance genes (ARGs) are pervasive in gut microbiota, but it remains unclear how often ARGs are transferred, particularly to pathogens. Traditionally, ARG spread is attributed to horizontal transfer mediated either by DNA transformation, bacterial conjugation or generalized transduction. However, recent viral metagenome (virome) analyses suggest that ARGs are frequently carried by phages, which is inconsistent with the traditional view that phage genomes rarely encode ARGs. Here we used exploratory and conservative bioinformatic strategies found in the literature to detect ARGs in phage genomes, and experimentally assessed a subset of ARG predicted using exploratory thresholds. ARG abundances in 1181 phage genomes were vastly overestimated using exploratory thresholds (421 predicted vs 2 known), due to low similarities and matches to protein unrelated to antibiotic resistance. Consistent with this, four ARGs predicted using exploratory thresholds were experimentally evaluated and failed to confer antibiotic resistance in Escherichia coli. Reanalysis of available human- or mouse-associated viromes for ARGs and their genomic context suggested that bona fide ARG attributed to phages in viromes were previously overestimated. These findings provide guidance for documentation of ARG in viromes, and reassert that ARGs are rarely encoded in phages

Design, Construction and In Situ Testing of a Muon Camera for Earth Science and Civil Engineering Applications

The MUST2 (MUon Survey Tomography based on Micromegas detectors for Unreachable Sites Technology) camera is based on a thin Time Projection Chamber read by a resistive Micromegas. This innovative combination presents interesting distinctive features compared to existing muon detection technologies. It allows a wide angular acceptance of the detector with a low weight and compact volume, well adapted for confined spaces or underground operation. The current work presents the results obtained during the calibration measurements at the reference site, the Low Background Noise Laboratory (LBNL). Preliminary results from field measurement campaign carried out at the dam overlooking the village of Saint-Saturnin-les-Apt (South-East of France) are presented and discussed

Operation Improvements and Emittance Reduction of the ESRF Booster

International audienceThe ESRF storage ring will be replaced by the Extremely Brilliant Source (EBS) in 2020 and the equilibrium emittance will decrease from the present 4 nmrad to 134 pmrad. The current injector system, composed by a linac and a synchrotron booster, will be used to inject into the new storage ring. To increase the injection efficiency in the new storage ring, three methods to reduce the horizontal emittance of the booster have been considered and tested. This paper presents the studies and achievements in terms of operation improvements and emittance reduction

Invasive fungal diseases in patients with autoimmune diseases: a case series from the French RESSIF network

International audienceObjectives We aimed to describe patients with autoimmune diseases (AID) developing invasive fungal disease (IFD) and identify factors associated with short-term mortality. Methods We analysed cases of IFD associated with AID from the surveillance network of invasive fungal diseases (Réseau de surveillance des infections fongiques invasives, RESSIF) registry of the French national reference centre for invasive mycoses. We studied association of AID-specific treatments with 30-day mortality. We analysed total lymphocyte and CD4-T cell counts in patients with Pneumocystis jirovecii pneumonia (PCP). Results From 2012 to 2018, 549 individuals with IFD and AID were included, mainly with PCP (n=227, 41.3%), fungemia (n=167, 30.4%) and invasive aspergillosis (n=84, 15.5%). Rheumatoid arthritis (RA) and anti-neutrophil cytoplasmic antibodies (ANCA)-associated vasculitides (AAV) were the most frequent AID in PCP (n=55 and 25, respectively) and invasive aspergillosis (n=15 and 10, respectively), inflammatory bowel diseases (IBDs) were predominant in fungemia (n=36). At IFD diagnosis, 365 (66.5%) patients received glucocorticoids (GCs), 285 (51.9%) immunosuppressants, 42 (7.7%) tumor necrosis factor (TNF)-α blockers, 75 (13.7%) other biologics. Mortality at 30 days was 28.1% (143/508). Fungemia and high-dose GCs were independently associated with higher 30-day mortality. In PCP patients, lymphopenia <1500/mm 3 was frequent (132/179, 73.7%) even if CD4+T cell count exceeded 200/mm 3 in 56/78 patients (71.8%) (median 472.5/mm 3 , IQR 160–858). Conclusion IFD associated with AID occurs primarily in RA, AAV and IBD, especially when treated with GCs and immunosuppressants. Mortality is high, especially for patients on high-dose GCs. Lymphopenia may help identify risk of PCP, but normal CD4+T cell count does not rule out the risk. Further studies are needed to assess the individual risk factors for IFD

Discovery of a big void in Khufu’s Pyramid by observation of cosmic-ray muons

International audienceThe Great Pyramid or Khufu’s Pyramid was built on the Giza Plateau (Egypt) during the IVth dynasty by the pharaoh Khufu (Cheops), who reigned from 2509 to 2483 BC$^1$ . Despite being one of the oldest and largest monuments on Earth, there is no consensus about how it was built. To better understand its internal structure, we imaged the pyramid using muons, which are by-products of cosmic rays that are only partially absorbed by stone. The resulting cosmic-ray muon radiography allows us to visualize the known and potentially unknown voids in the pyramid in a non-invasive way. Here we report the discovery of a large void (with a cross section similar to the Grand Gallery and a length of 30m minimum) above the Grand Gallery, which constitutes the first major inner structure found in the Great Pyramid since the 19$^{th}$ century. This void, named ScanPyramids Big Void, was first observed with nuclear emulsion films installed in the Queen’s chamber (Nagoya University), then confirmed with scintillator hodoscopes set up in the same chamber (KEK) and re-confirmed with gas detectors12 outside of the pyramid (CEA)This large void has therefore been detected with a high confidence by three different muon detection technologies andthree independent analyses. These results constitute a breakthrough for the understanding of Khufu’s Pyramid and its internal structure. While there is currently no information about the role of this void, these findings show how modern particle physics can shed new light on the world’s archaeological heritag