Drone Deployment Algorithms for Effective Communication Establishment in Disaster Affected Areas

Communication establishment is crucial for rescue operations in disaster affected areas. A standard tool for communication is the use of cell phones. However, they can be useless in situations where the cellular network’s base stations are damaged in a disaster. A contemporary approach to re-establishing a communication network is by hosting base stations in drones. However, low battery life and difficulty in calculating the number of drones needed in different terrains are limitations of the above approach. This paper introduces a novel terrain-aware algorithm that calculates the minimum number of drones needed to cover an area with no voids in the network coverage. Our method ensures that the drones are deployed at optimal heights to maximize the average leftover energy in the network. We apply the algorithm for an actual location in Pettimudi, India and find the optimal number and positions of the drones to cover the area effectively without voids. In addition, we provide a simulation of the the communication establishment using above drones, and our experiments yield an average network efficiency of 98%, showing the effectiveness of our method

Drone Deployment Algorithms for Effective Communication Establishment in Disaster Affected Areas

Communication establishment is crucial for rescue operations in disaster affected areas. A standard tool for communication is the use of cell phones. However, they can be useless in situations where the cellular network’s base stations are damaged in a disaster. A contemporary approach to re-establishing a communication network is by hosting base stations in drones. However, low battery life and difficulty in calculating the number of drones needed in different terrains are limitations of the above approach. This paper introduces a novel terrain-aware algorithm that calculates the minimum number of drones needed to cover an area with no voids in the network coverage. Our method ensures that the drones are deployed at optimal heights to maximize the average leftover energy in the network. We apply the algorithm for an actual location in Pettimudi, India and find the optimal number and positions of the drones to cover the area effectively without voids. In addition, we provide a simulation of the the communication establishment using above drones, and our experiments yield an average network efficiency of 98%, showing the effectiveness of our method

Electroporation-mediated delivery of the FER Gene in the Resolution of Trauma-related Fatal Pneumonia

Injured patients with lung contusion (LC) are at risk of developing bacterial pneumonia (PNA) followed by sepsis and death. A recent genome-wide association study (GWAS) showed FER gene expression positively correlating with survival rates among individuals with above conditions. We sought to determine whether electroporation (EP)-mediated delivery of FER gene could indeed improve survival, in a lethal model of combined LC and PNA. C57BL/6 mice sustained unilateral LC, which preceded a 500 Klebsiella colony forming unit (CFU) inoculation by 6 h. In-between these insults, human FER plasmid (pFER) was introduced into the lungs followed by eight EP pulses applied externally (10 ms at 200 V cm-1). Control groups included EP of empty vector (pcDNA3) or Na+/K+-ATPase genes (pPump) and no treatment (LC+PNA). We recorded survival, histology, lung mechanics, bronchial alveolar lavage (BAL) fluid, FER and inflammatory gene expression and bacteriology. The data show that 7-day survival was significantly improved by pFER compared with control groups. pFER increased BAL monocytes and activated antibacterial response genes (nitric oxide synthase (NOS), Fizz). pFER treatment showed decreased lung and blood Klebsiella counts reaching, in some cases, complete sterilization. In conclusion, FER gene delivery promoted survival in LC+PNA mice via recruitment of activated immune cells, improving efficiency of bacterial clearance within contused lung.http://deepblue.lib.umich.edu/bitstream/2027.42/177331/2/Electroporation-mediated delivery of the FER gene in the resolution of trauma-related fatal pneumonia.pdfPublished versionDescription of Electroporation-mediated delivery of the FER gene in the resolution of trauma-related fatal pneumonia.pdf : Accepted versio

An Unusual Transduction Pathway in Human Tonic Smooth Muscle Myosin

The motor protein myosin binds actin and ATP, producing work by causing relative translation of the proteins while transducing ATP free energy. Smooth muscle myosin has one of four heavy chains encoded by the MYH11 gene that differ at the C-terminus and in the active site for ATPase due to alternate splicing. A seven-amino-acid active site insert in phasic muscle myosin is absent from the tonic isoform. Fluorescence increase in the nucleotide sensitive tryptophan (NST) accompanies nucleotide binding and hydrolysis in several myosin isoforms implying it results from a common origin within the motor. A wild-type tonic myosin (smA) construct of the enzymatic head domain (subfragment 1 or S1) has seven tryptophan residues and nucleotide-induced fluorescence enhancement like other myosins. Three smA mutants probe the molecular basis for the fluorescence enhancement. W506+ contains one tryptophan at position 506 homologous to the NST in other myosins. W506F has the native tryptophans except phenylalanine replaces W506, and W506+(Y499F) is W506+ with phenylalanine replacing Y499. W506+ lacks nucleotide-induced fluorescence enhancement probably eliminating W506 as the NST. W506F has impaired ATPase activity but retains nucleotide-induced fluorescence enhancement. Y499F replacement in W506+ partially rescues nucleotide sensitivity demonstrating the role of Y499 as an NST facilitator. The exceptional response of W506 to active site conformation opens the possibility that phasic and tonic isoforms differ in how influences from active site ATPase propagate through the protein network