66 research outputs found
A Comparison of Two Pelvic Floor Muscle Training Programs in Females with Stress Urinary Incontinence: A Pilot Study
Background: Stress urinary incontinence (SUI) is a condition affecting millions of Americans. Few studies have assessed the benefits of different exercises involved in pelvic floor muscle training (PFMT). Purposte: The purpose of this study was to compare the effects of a traditional PFMT program to an assisted pelvic floor muscle training (APFMT) program that included contraction of hip musculature
Nivel de conocimiento sobre la norma técnica de planificación familiar en internas de la E.P de Obstetricia, Cusco 2023
La investigación “Nivel de conocimientos sobre la norma técnica de planificación
familiar en internas de la E.P de Obstetricia, Cusco 2023” tiene como objetivo medir el
nivel de conocimientos sobre la norma técnica de planificación familiar en internas de la
E.P de Obstetricia, Cusco 2023.
El estudio de tipo cuantitativo, descriptivo con un diseño no experimental, de corte
transversal, prospectivo. Con una muestra de 60 internas de Obstetricia aplicando un
cuestionario.
Se trabajó con las variables de estudio: norma técnica de planificación familiar que está
compuesto por los componentes de prestación, organización y gestión; Así mismo se
identificaron variables intervinientes: características sociodemográficas clasificado en
edad y sexo.
Como resultado se obtuvo que el nivel de conocimiento sobre la norma técnica de
planificación familiar fue 82% regular, bueno en un 15%, y deficiente en un 3%; en
cuanto el conocimiento de los componentes de prestación fue regular en un 50%, bueno
en un 48%, y deficiente en un 2%; sobre los componentes de organización el nivel de
conocimiento fue regular en un 75%, deficiente 12% y bueno 13%, finalmente nivel de
conocimiento sobre el componentes de gestión fue regular 72%, deficiente 15%, y bueno
13%.
Conclusión: De acuerdo al estudio realizado se encontró un conocimiento regular de los
3 componentes de prestación de la norma técnica de planificación familiar observando
que se necesita más énfasis en el tema de planificación familiar.The research "Level of knowledge about the technical norm of family planning in inmates
of the E.P of Obstetrics, Cusco 2023" had as objective to determine the level of
knowledge about the technical norm of family planning in inmates of the E.P of obstetrics,
Cusco 2023.
The study was quantitative, descriptive with an experimental, relational, cross-sectional,
prospective design. Where 60 obstetric interns were surveyed applying a questionnaire.
We worked with the study variables: technical norm of family planning that is composed
of the components of provision, organization and management; likewise, intervening
variables were identified: sociodemographic characteristics classified by age and sex.
As a result, it was obtained that the level of knowledge about the technical standard of
family planning was 80% regular, 15% good, and 5% deficient; as far as the knowledge
of the benefit components was regular in 65%, good in 30%, and deficient in 5%;
Regarding the organizational components, the level of knowledge was regular in 83%,
deficient 10% and good 7%, finally the level of knowledge about the management
components was regular 82%, deficient 15%, and good 3%.
Conclusion: According to the study carried out, a regular knowledge of the 3 components
of provision of the technical norm of family planning was found.Planificación familia
The Vehicle, Spring 1983
Vol. 24, No. 2
Table of Contents
A-B-C-D-E-F-G-H....Beth Kennypage 1
Contemporary IssuesBrook Wilsonpage 1
BlackJohn Stockmanpage 2
BeatGraham Lewispage 2
Catholic DazeSuzanne Hornpage 4
AfricaGraham Lewispage 5
The Friendly SkiesRajendra Sinhanpage 5
BreadKen Kempckepage 7
PhotographLinda Fraembspage 8
SnapshotMaggie Kennedypage 9
PoemAnne Smithpage 9
Activities on IceKerri Mahatpage 11
Beecham\u27s Orchard And VineyardBecky Lawsonpage 11
PoemKarri Mahatpage 12
Sneak PreviewsMaggie Kennedypage 12
ZooKen Kempckepage 12
PhotographNick Haskettpage 13
The Slave HouseCraig Barnespage 13
The Nomad Preacher\u27s SermonStacey Flanniganpage 16
Owl Creek RevisitedScott Graypage 16
Thought On CopperGraham Lewispage 20
OutfielderKen Kempckepage 20
HoneymoonJohn Stockmanpage 21
Candy Wrapper Dream GirlStacey Flanniganpage 21
PhotographLinda Fraembspage 22
October DreamMarlene Weekspage 23
IndistinctionStacey Flanniganpage 24
Taking InventorySara Farrispage 24
Flying In From K-Mart, NebraskaMichelle Mitchellpage 25
PhotographNick Haskettpage 26
Bone ChinaMichelle Mitchellpage 27
She Was A DollNick Haskettpage 30
The Seventh DayGeoffrey Andrespage 31
Blade Of Grass (On A Golf Course)Ken Kempckepage 31
PoemKen Kempckepage 32
Cigarette SmokeJean M. Davispage 33
Future LoveR. Lawsonpage 34
PhotographNick Haskettpage 35
Dancing In The StreetBetsy Acklinpage 35
PhotographLinda Fraembspage 38
CleoMarlene Weekspage 39
Teddy BearKen Kempckepage 39
PreludeBecky Lawsonpage 40https://thekeep.eiu.edu/vehicle/1043/thumbnail.jp
Long-Distance Three-Color Neuronal Tracing in Fixed Tissue Using NeuroVue Dyes
Dissecting development of neuronal connections is critical for understanding neuronal function in both normal and diseased states. Charting the development of the multitude of connections is a monumental task, since a given neuron typically receives hundreds of convergent inputs from other neurons and provides divergent outputs for hundreds of other neurons. Although progress is being made utilizing various mutants and/or genetic constructs expressing fluorescent proteins like GFP, substantial work remains before a database documenting the development and final location of the neuronal pathways in an adult animal is completed. The vast majority of developing neurons cannot be specifically labeled with antibodies and making specific GFP-expressing constructs to tag each of them is an overwhelming task. Fortunately, fluorescent lipophilic dyes have emerged as very useful tools to systematically compare changes in neuronal networks between wild-type and mutant mice. These dyes diffuse laterally along nerve cell membranes in fixed preparations, allowing tracing of the position of a given neuron within the neuronal network in murine mutants fixed at various stages of development. Until recently, however, most evaluations have been limited to one, or at most, two color analyses. We have previously reported three color neuronal profiling using the novel lipophilic dyes NeuroVue (NV) Green, Red and Maroon (Fritzsch et al., Brain. Res. Bull. 66:249–258, 2005). Unfortunately such three color experiments have been limited by the fact that NV Green and its brighter successor, NV Emerald, both exhibit substantially decreased signal intensities when times greater than 48 hours at 37°C are required to achieve neuronal profile filling (unpublished observations). Here we describe a standardized test system developed to allow comparison of candidate dyes and its use to evaluate a series of 488 nm-excited green-emitting lipophilic dyes. The best of these, NV Jade, has spectral properties well matched to NV Red and NV Maroon, better solubility in DMF than DiO or DiA, improved thermostability compared with NV Emerald, and the ability to fill neuronal profiles at rates of 1 mm per day for periods of at least 5 days. Use of NV Jade in combination with NV Red and NV Maroon substantially improves the efficiency of connectional analysis in complex mutants and transgenic models where limited numbers of specimens are available
Electroporation-Induced Electrosensitization
BACKGROUND: Electroporation is a method of disrupting the integrity of cell membrane by electric pulses (EPs). Electrical modeling is widely employed to explain and study electroporation, but even most advanced models show limited predictive power. No studies have accounted for the biological consequences of electroporation as a factor that alters the cell's susceptibility to forthcoming EPs. METHODOLOGY/PRINCIPAL FINDINGS: We focused first on the role of EP rate for membrane permeabilization and lethal effects in mammalian cells. The rate was varied from 0.001 to 2,000 Hz while keeping other parameters constant (2 to 3,750 pulses of 60-ns to 9-µs duration, 1.8 to 13.3 kV/cm). The efficiency of all EP treatments was minimal at high rates and started to increase gradually when the rate decreased below a certain value. Although this value ranged widely (0.1-500 Hz), it always corresponded to the overall treatment duration near 10 s. We further found that longer exposures were more efficient irrespective of the EP rate, and that splitting a high-rate EP train in two fractions with 1-5 min delay enhanced the effects severalfold. CONCLUSIONS/SIGNIFICANCE: For varied experimental conditions, EPs triggered a delayed and gradual sensitization to EPs. When a portion of a multi-pulse exposure was delivered to already sensitized cells, the overall effect markedly increased. Because of the sensitization, the lethality in EP-treated cells could be increased from 0 to 90% simply by increasing the exposure duration, or the exposure dose could be reduced twofold without reducing the effect. Many applications of electroporation can benefit from accounting for sensitization, by organizing the exposure either to maximize sensitization (e.g., for sterilization) or, for other applications, to completely or partially avoid it. In particular, harmful side effects of electroporation-based therapies (electrochemotherapy, gene therapies, tumor ablation) include convulsions, pain, heart fibrillation, and thermal damage. Sensitization can potentially be employed to reduce these side effects while preserving or increasing therapeutic efficiency
The Comprehensive Post-Acute Stroke Services (COMPASS) study: design and methods for a cluster-randomized pragmatic trial
Background: Patients discharged home after stroke face significant challenges managing residual neurological deficits, secondary prevention, and pre-existing chronic conditions. Post-discharge care is often fragmented leading to increased healthcare costs, readmissions, and sub-optimal utilization of rehabilitation and community services. The COMprehensive Post-Acute Stroke Services (COMPASS) Study is an ongoing cluster-randomized pragmatic trial to assess the effectiveness of a comprehensive, evidence-based, post-acute care model on patient-centered outcomes.
Methods: Forty-one hospitals in North Carolina were randomized (as 40 units) to either implement the COMPASS care model or continue their usual care. The recruitment goal is 6000 patients (3000 per arm). Hospital staff ascertain and enroll patients discharged home with a clinical diagnosis of stroke or transient ischemic attack. Patients discharged from intervention hospitals receive 2-day telephone follow-up; a comprehensive clinic visit within 2 weeks that includes a neurological evaluation, assessments of social and functional determinants of health, and an individualized COMPASS Care PlanTM integrated with a community-specific resource database; and additional follow-up calls at 30 and 60 days post-stroke discharge. This model is consistent with the Centers for Medicare and Medicaid Services transitional care management services provided by physicians or advanced practice providers with support from a nurse to conduct patient assessments and coordinate follow-up services. Patients discharged from usual care hospitals represent the control group and receive the standard of care in place at that hospital. Patient-centered outcomes are collected from telephone surveys administered at 90 days. The primary endpoint is patient-reported functional status as measured by the Stroke Impact Scale 16. Secondary outcomes are: caregiver strain, all-cause readmissions, mortality, healthcare utilization, and medication adherence. The study engages patients, caregivers, and other stakeholders (including policymakers, advocacy groups, payers, and local community coalitions) to advise and support the design, implementation, and sustainability of the COMPASS care model.
Discussion: Given the high societal and economic burden of stroke, identifying a care model to improve recovery, independence, and quality of life is critical for stroke survivors and their caregivers. The pragmatic trial design provides a real-world assessment of the COMPASS care model effectiveness and will facilitate rapid implementation into clinical practice if successful
An original phylogenetic approach identified mitochondrial haplogroup T1a1 as inversely associated with breast cancer risk in BRCA2 mutation carriers
Introduction: Individuals carrying pathogenic mutations in the BRCA1 and BRCA2 genes have a high lifetime risk of breast cancer. BRCA1 and BRCA2 are involved in DNA double-strand break repair, DNA alterations that can be caused by exposure to reactive oxygen species, a main source of which are mitochondria. Mitochondrial genome variations affect electron transport chain efficiency and reactive oxygen species production. Individuals with different mitochondrial haplogroups differ in their metabolism and sensitivity to oxidative stress. Variability in mitochondrial genetic background can alter reactive oxygen species production, leading to cancer risk. In the present study, we tested the hypothesis that mitochondrial haplogroups modify breast cancer risk in BRCA1/2 mutation carriers. Methods: We genotyped 22,214 (11,421 affected, 10,793 unaffected) mutation carriers belonging to the Consortium of Investigators of Modifiers of BRCA1/2 for 129 mitochondrial polymorphisms using the iCOGS array. Haplogroup inference and association detection were performed using a phylogenetic approach. ALTree was applied to explore the reference mitochondrial evolutionary tree and detect subclades enriched in affected or unaffected individuals. Results: We discovered that subclade T1a1 was depleted in affected BRCA2 mutation carriers compared with the rest of clade T (hazard ratio (HR) = 0.55; 95% confidence interval (CI), 0.34 to 0.88; P = 0.01). Compared with the most frequent haplogroup in the general population (that is, H and T clades), the T1a1 haplogroup has a HR of 0.62 (95% CI, 0.40 to 0.95; P = 0.03). We also identified three potential susceptibility loci, including G13708A/rs28359178, which has demonstrated an inverse association with familial breast cancer risk. Conclusions: This study illustrates how original approaches such as the phylogeny-based method we used can empower classical molecular epidemiological studies aimed at identifying association or risk modification effects.Peer reviewe
- …