Monitoring Red Deer and Roe Deer Population Density in Yedigoller-Yesiloz Wildlife Reserves in Turkey

International audienceCJUE 4 déc. 2014, aff. C-295/13, D. 2015. 7

Monitoring Red Deer and Roe Deer Population Density in Yedigoller-Yesiloz Wildlife Reserves in Turkey

Wildlife inventories are very new in Turkey. Thus, there is no enough data about red deer and roe deer population. However, these studies have gained momentum in recent years. In this study, it is aimed to determine and to monitor status of red deer and roe deer population densities. In the study, it was used a kind of drive count which was combined with point count. The study was conducted between 2003 and 2010 in October, once a year. Red deer and roe deer densities were determined between 0.45 and 1.19 red deer/km(2); 1.48 and 2.05 roe deer/km(2). Also, average of annual growth rates were estimated for red deer (0.135) and roe deer (-0.0059). The study showed that whilst the red deer population has been increasing, roe deer population has been decreasing in long term period

Arthropod diversity in pure oak forests of coppice origin in northern Thrace (Turkey)

Oak (Quercus spp.) forests are among the most important forest types in Turkey. In the past, oak forests were managed through coppice clear-cutting, but in recent decades they have mostly been converted to high forest. This study was aimed at explaining how arthropod diversity is affected during conversion from coppice to high oak forest and during the early stages of coppice succession. We tested the hypothesis that arthropod richness, abundance and diversity in coppice oak sites varied according to stand age and a number of other forest characteristics. Arthropod communities were sampled in 50 plots using four different methods: pitfall traps, sweep nets, sticky cards and cloth shaking. A total of 13 084 individuals were collected and classified into 193 Recognizable Taxonomic Units (RTUs), with the most RTUs and the greatest number of specimens captured by sweep netting. We identified 17 taxa within RTU’s with more than 1% of the captured arthropods, which constituted 75% of the total specimens. The number of RTUs varied significantly according to trap type. Arthropod richness and Shannon-Wiener biodiversity index (H′) increased with elevation and precipitation. In young (1-40 yrs-old) and middle-aged (41-80 yrs) stands, arthropod biodiversity was not significantly affected by stand type, but slightly increased with diameter at breast height and tree height. Forest characteristics, such as the litter layer, understory and crown diameter, weakly influenced arthropod richness and abundance. Cluster analysis revealed that stand types and trap types differed taxonomically. Principal component analysis showed that stand types were clearly separated by the stand parameters measured. Insect families (Formicidae, Thripidae, Lygaeidae, Dolichopodidae, Luaxanidae, Cicadellidae and Ichneumonidae) could potentially be used as indicators of coppice oak conditions. As the coppice oak changes to mature forest, further studies are needed to better assess the relation between arthropods, forest types and structural characteristics of stands

AB012. Transcriptional and chromatin profiling reveals the molecular architecture and druggable vulnerabilities of thymic epithelial tumors (TETs)

Thymic epithelial tumors (TETs) have been profiled to the present moment mainly through several analyses of FFPE samples. Despite the leap forward brought by the TCGA, several questions remain still unsolved. Among these, TETs are characterized by a strong component of immune infiltrate which makes the transcriptomic analyses conducted so far scarcely interpretable to profile stromal subpopulations constitutive of the tumor. Furthermore, rarely correspondent healthy tissue is available due to the lipomatous atrophy of aged thymi. Therefore, the recent report of (I) isolation, (II) propagation (III) and characterization of human thymic epithelial cells (TECs) and their capacity to reconstitute the functional organ ex vivo and in vivo, represents a novel approach to study the biology of both healthy and neoplastic thymi. Human thymic biopsies (both healthy and neoplastic) were digested and plated on a lethally irradiated murine feeder layer. Both RNA-Seq and CUTANDTAG were performed on cultivated TECs at different passages. Cultured TECs were injected with human thymic interstitial cells into rat decellularized scaffolds and cultivated for 10–12 days. sc-RNA Seq is currently being performed on both healthy and neoplastic thymic mini-organs and their correspondent primary tissues. Here show that we successfully cultivated a cohort of 21 clonogenic TECs in vitro including adult neoplastic TECs, their non-tumoral counterpart and pediatric TECs. We show that at the transcriptome level each class of TECs clusters independently and that neoplastic TECs belong to the same cloud independently from thymoma histotype. Around 1,400 differentially expressed genes (DEGs) can be found when comparing adult neoplastic and non-neoplastic counterpart, among which around 70 are transcription factors. Importantly, we prove for the first time that clonogenic TECs derived from TETs can repopulate a decellularized rat scaffold and recreate a 3D architecture mimicking the primary tumor. This work demonstrates that this culture system allows the expansion of clonogenic TECs from both tumor samples and their non-tumoral counterpart. Those cells, when transplanted into decellularized thymi, reproduce the architecture of the primary tissue, showing that TETs contain progenitor/stem epithelial cells. We are currently characterizing TECs at the transcriptomic and epigenomic level with aim of identifying new druggable targets prior to clinical trials

Natural-based nanocomposites for bone tissue engineering and regenerative medicine: a review

Tissue engineering and regenerative medicine has been providing exciting technologies for the development of functional substitutes aimed to repair and regenerate damaged tissues and organs. Inspired by the hierarchical nature of bone, nanostructured biomaterials are gaining a singular attention for tissue engineering, owing their ability to promote cell adhesion and proliferation, and hence new bone growth, compared with conventional microsized materials. Of particular interest are nanocomposites involving biopolymeric matrices and bioactive nanosized fi llers. Biodegradability, high mechanical strength, and osteointegration and formation of ligamentous tissue are properties required for such materials. Biopolymers are advantageous due to their similarities with extracellular matrices, specifi c degradation rates, and good biological performance. By its turn, calcium phosphates possess favorable osteoconductivity, resorbability, and biocompatibility. Herein, an overview on the available natural polymer/calcium phosphate nanocomposite materials, their design, and properties is presented. Scaffolds, hydrogels, and fi bers as biomimetic strategies for tissue engineering, and processing methodologies are described. The specifi c biological properties of the nanocomposites, as well as their interaction with cells, including the use of bioactive molecules, are highlighted. Nanocomposites in vivo studies using animal models are also reviewed and discussed.  The research leading to this work has received funding from the European Union's Seventh Framework Programme (FP7/2007-2013) under grant agreement no REGPOT-CT2012-316331-POLARIS, and from QREN (ON.2 - NORTE-01-0124-FEDER-000016) cofinanced by North Portugal Regional Operational Program (ON.2 - O Novo Norte), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF)

Monitoring Red Deer and Roe Deer Population Density in Yedigoller-Yesiloz Wildlife Reserves in Turkey

Wildlife inventories are very new in Turkey. Thus, there is no enough data about red deer and roe deer population. However, these studies have gained momentum in recent years. In this study, it is aimed to determine and to monitor status of red deer and roe deer population densities. In the study, it was used a kind of drive count which was combined with point count. The study was conducted between 2003 and 2010 in October, once a year. Red deer and roe deer densities were determined between 0.45 and 1.19 red deer/km(2); 1.48 and 2.05 roe deer/km(2). Also, average of annual growth rates were estimated for red deer (0.135) and roe deer (-0.0059). The study showed that whilst the red deer population has been increasing, roe deer population has been decreasing in long term period

Jag vill vara en av dem men jag förstår inte dem - Kvalitativ studie om lärare och elevers tankar om förberedelseklass

Intentionerna med vår undersökning har varit att undersöka nyanlända elevers respektive lärarnas uppfattning om förberedelseklassen. Vi redogör för elevernas förutsättningar och behov, samt hur de kämpar för att anpassa sig i den nya skolan. Vi beskriver även lärarnas tankar om elevernas behov. I vår slutdiskussion har vi dragit slutsatsen att det är mycket som behöver bli bättre i skolan. Vi efterlyser bättre samarbete mellan förberedelseklasslärarna och övriga personal i skolan, samt bättre kontakt med modersmålslärare och föräldrar. Vi anser att ett bra samarbete och bättre inställning till nyanlända elever skulle bidra till ökad utveckling och lärande