A small angle X-ray scattering study on high pH silica precipitations

Silica precipitations were carried out at high pH (7-10) and elevated temperatures (60-90 degrees C) by means of simultaneous dosing of diluted water glass and sulphuric acid into a stirred thermostatted reaction vessel. In order to investigate the development of the vulnerable silica structures during the processes, every 5-10 min small samples were taken from the reaction mixture and analysed using small angle X-ray scattering. It was found that a narrow particle size distribution was maintained throughout the entire preparation procedure (or at least from about 1 h until the end of the process), despite the fact that the primary silica particles were growing continuously. In addition, a continuous decrease in the total number of primary particles was observed, indicating that the primary particles were involved in clustering and aging processes, and that (except for the initial reaction stages) newly dosed silica does not form new particles but is deposited onto the already existing particles. Ostwald ripening, which is regarded as the most important aging mechanism, probably proceeds relatively rapidly under the applied process conditions, leading to a persistent high degree of monodispersit

Reconstructing mass profiles of simulated galaxy clusters by combining Sunyaev-Zeldovich and X-ray images

We present a method to recover mass profiles of galaxy clusters by combining data on thermal Sunyaev-Zeldovich (tSZ) and X-ray imaging, thereby avoiding to use any information on X-ray spectroscopy. This method, which represents a development of the geometrical deprojection technique presented in Ameglio et al. (2007), implements the solution of the hydrostatic equilibrium equation. In order to quantify the efficiency of our mass reconstructions, we apply our technique to a set of hydrodynamical simulations of galaxy clusters. We propose two versions of our method of mass reconstruction. Method 1 is completely model-independent, while Method 2 assumes instead the analytic mass profile proposed by Navarro et al. (1997) (NFW). We find that the main source of bias in recovering the mass profiles is due to deviations from hydrostatic equilibrium, which cause an underestimate of the mass of about 10 per cent at r_500 and up to 20 per cent at the virial radius. Method 1 provides a reconstructed mass which is biased low by about 10 per cent, with a 20 per cent scatter, with respect to the true mass profiles. Method 2 proves to be more stable, reducing the scatter to 10 per cent, but with a larger bias of 20 per cent, mainly induced by the deviations from equilibrium in the outskirts. To better understand the results of Method 2, we check how well it allows to recover the relation between mass and concentration parameter. When analyzing the 3D mass profiles we find that including in the fit the inner 5 per cent of the virial radius biases high the halo concentration. Also, at a fixed mass, hotter clusters tend to have larger concentration. Our procedure recovers the concentration parameter essentially unbiased but with a scatter of about 50 per cent.Comment: 13 pages, 11 figures, submitted to MNRA

Spatially resolved multiomics of human cardiac niches

The function of a cell is defined by its intrinsic characteristics and its niche: the tissue microenvironment in which it dwells. Here we combine single-cell and spatial transcriptomics data to discover cellular niches within eight regions of the human heart. We map cells to microanatomical locations and integrate knowledge-based and unsupervised structural annotations. We also profile the cells of the human cardiac conduction system1. The results revealed their distinctive repertoire of ion channels, G-protein-coupled receptors (GPCRs) and regulatory networks, and implicated FOXP2 in the pacemaker phenotype. We show that the sinoatrial node is compartmentalized, with a core of pacemaker cells, fibroblasts and glial cells supporting glutamatergic signalling. Using a custom CellPhoneDB.org module, we identify trans-synaptic pacemaker cell interactions with glia. We introduce a druggable target prediction tool, drug2cell, which leverages single-cell profiles and drug-target interactions to provide mechanistic insights into the chronotropic effects of drugs, including GLP-1 analogues. In the epicardium, we show enrichment of both IgG+ and IgA+ plasma cells forming immune niches that may contribute to infection defence. Overall, we provide new clarity to cardiac electro-anatomy and immunology, and our suite of computational approaches can be applied to other tissues and organs

Geographical and temporal distribution of SARS-CoV-2 clades in the WHO European Region, January to June 2020

We show the distribution of SARS-CoV-2 genetic clades over time and between countries and outline potential genomic surveillance objectives. We applied three available genomic nomenclature systems for SARS-CoV-2 to all sequence data from the WHO European Region available during the COVID-19 pandemic until 10 July 2020. We highlight the importance of real-time sequencing and data dissemination in a pandemic situation. We provide a comparison of the nomenclatures and lay a foundation for future European genomic surveillance of SARS-CoV-2.Peer reviewe