Disruption of Mitochondrial DNA Replication in Drosophila Increases Mitochondrial Fast Axonal Transport In Vivo

Mutations in mitochondrial DNA polymerase (pol γ) cause several progressive human diseases including Parkinson's disease, Alper's syndrome, and progressive external ophthalmoplegia. At the cellular level, disruption of pol γ leads to depletion of mtDNA, disrupts the mitochondrial respiratory chain, and increases susceptibility to oxidative stress. Although recent studies have intensified focus on the role of mtDNA in neuronal diseases, the changes that take place in mitochondrial biogenesis and mitochondrial axonal transport when mtDNA replication is disrupted are unknown. Using high-speed confocal microscopy, electron microscopy and biochemical approaches, we report that mutations in pol γ deplete mtDNA levels and lead to an increase in mitochondrial density in Drosophila proximal nerves and muscles, without a noticeable increase in mitochondrial fragmentation. Furthermore, there is a rise in flux of bidirectional mitochondrial axonal transport, albeit with slower kinesin-based anterograde transport. In contrast, flux of synaptic vesicle precursors was modestly decreased in pol γ−α mutants. Our data indicate that disruption of mtDNA replication does not hinder mitochondrial biogenesis, increases mitochondrial axonal transport, and raises the question of whether high levels of circulating mtDNA-deficient mitochondria are beneficial or deleterious in mtDNA diseases

eHealth to improve patient outcome in rehabilitating myocardial infarction patients

Introduction: Cardiac rehabilitation is aimed at risk factor modification and improving quality of life. eHealth has a couple of potential benefits to improve this aim. The primary purpose of this review is to summarize available literature for eHealth strategies that have been investigated in randomized controlled trials in post-myocardial infarction (MI) patients. The second purpose of this review is to investigate the clinical effectiveness in post-MI patients. Areas covered: The literature was searched using PubMed. Randomized controlled trials (RCTs) describing interventions in patients that had experienced an ST-elevation myocardial infarction or non-ST acute coronary syndrome were eligible for inclusion. Fifteen full-texts were included and their results are described in this review. These RCTs described interventions that used remote coaching or remote monitoring in post-MI patients. Most interventions resulted in an improved cardiovascular risk profile. Remote coaching had a positive effect on activity and dietary intake. Expert opinion: eHealth might be clinically beneficial in post-MI patients, particularly for risk estimation. Moreover, eHealth as a tool for remote coaching on activity is a good addition to traditional cardiac rehabilitation programs. Further research needs to corroborate these findings

Less bleeding by omitting aspirin in non-ST-segment elevation acute coronary syndrome patients:Rationale and design of the LEGACY study

Background: Early aspirin withdrawal, also known as P2Y12-inhibitor monotherapy, following percutaneous coronary intervention (PCI) for non-ST-segment elevation acute coronary syndrome (NSTE-ACS) can reduce bleeding without a trade-off in efficacy. Still the average daily bleeding risk is highest during the first months and it remains unclear if aspirin can be omitted immediately following PCI. Methods: The LEGACY study is an open-label, multicenter randomized controlled trial evaluating the safety and efficacy of immediate P2Y12-inhibitor monotherapy versus dual antiplatelet therapy (DAPT) for 12 months in 3,090 patients. Patients are randomized immediately following successful PCI for NSTE-ACS to 75-100 mg aspirin once daily versus no aspirin. The primary hypothesis is that immediately omitting aspirin is superior to DAPT with respect to major or minor bleeding defined as Bleeding Academic Research Consortium type 2, 3, or 5 bleeding, while maintaining noninferiority for the composite of all-cause mortality, myocardial infarction and stroke compared to DAPT. Conclusions: The LEGACY study is the first randomized study that is specifically designed to evaluate the impact of immediately omitting aspirin, and thus treating patients with P2Y12-inhibitor monotherapy, as compared to DAPT for 12 months on bleeding and ischemic events within 12 months following PCI for NSTE-ACS

Global patterns and predictors of microplastic occurrence and abundance in lentic systems

The majority of microplastic research has focused on seawater, with fewer than 4% of microplasticsrelated studies occurring on freshwaters. The limited available information suggests that the abundance of microplastics in freshwaters is often as high or even higher than marine environments. However, comprehensive investigations on occurrence and fate of microplastics in freshwaters are scarce and highly fragmented, partly because detection and identification of microplastic particles is rather complex. In addition, up to now, harmonized and standardized protocols for the sampling and analysis of microplastics in freshwaters do not exist, and studies with different research aims and hypotheses often report unstandardized results, making comparison among studies difficult. In the present study, we performed the first global standardized sampling and analysis effort to investigate the occurrence and distribution of microplastics in surface water of lakes and reservoirs with different anthropogenic impacts. Participants aim to collect water samples of freshwater systems with different features (e.g., area, depth, thermal behavior, watershed), following a common protocol. This 18 establishes the collection of samples by horizontal trawling of a plankton net and, after treatment with hydrogen peroxide, the polymer identification through micro-Raman spectroscopy. This GLEON project will allow obtaining comparable data about microplastic contamination in different freshwater systems around the globe. With this global dataset, our goals are to determine whether a relationship exists between the abundance of microplastics and the waterbody/watershed attributes and understand which factors are likely to influence the occurrence of microplastics in surface water of lentic systems.info:eu-repo/semantics/publishedVersio

Global patterns and predictors of microplastic occurrence and abundance in lentic systems

The majority of microplastic research has focused on seawater, with fewer than 4% of microplasticsrelated studies occurring on freshwaters. The limited available information suggests that theabundance of microplastics in freshwaters is often as high or even higher than marine environments.However, comprehensive investigations on occurrence and fate of microplastics in freshwaters arescarce and highly fragmented, partly because detection and identification of microplastic particles israther complex. In addition, up to now, harmonized and standardized protocols for the sampling andanalysis of microplastics in freshwaters do not exist, and studies with different research aims andhypotheses often report unstandardized results, making comparison among studies difficult. In thepresent study, we performed the first global standardized sampling and analysis effort to investigatethe occurrence and distribution of microplastics in surface water of lakes and reservoirs with differentanthropogenic impacts. Participants aim to collect water samples of freshwater systems with differentfeatures (e.g., area, depth, thermal behavior, watershed), following a common protocol. This establishes the collection of samples by horizontal trawling of a plankton net and, after treatmentwith hydrogen peroxide, the polymer identification through micro-Raman spectroscopy. This GLEONproject will allow obtaining comparable data about microplastic contamination in different freshwatersystems around the globe. With this global dataset, our goals are to determine whether a relationshipexists between the abundance of microplastics and the waterbody/watershed attributes andunderstand which factors are likely to influence the occurrence of microplastics in surface water oflentic systems.Fil: Nava, Veronica. University Of Milano-Bicocca; ItaliaFil: Frezzotti, Maria Luce. University Of Milano-Bicocca; ItaliaFil: Aherne, Julian. Trent University; CanadáFil: Alfonso, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; ArgentinaFil: AntãoGeraldes, Ana M.. Instituto Politécnico De Bragança; PortugalFil: Attermeyer, Katrin. No especifíca;Fil: Bah, Abdou R.. City University of New York; Estados UnidosFil: Bao, Roberto. Universidad da Coruña; EspañaFil: Bartrons, Mireia. Universidad Politécnica de Catalunya; EspañaFil: Berger, Stella A.. Leibniz Institute for Zoo and Wildlife Research; AlemaniaFil: Biernaczyk, Marcin. No especifíca;Fil: Breider, Florian. Institute Of Environmental Engineering; SueciaFil: Brookes, Justin. University of Adelaide; AustraliaFil: Cañedo Argüelles, Miguel. Universidad de Barcelona; EspañaFil: Canle, Moisés. Universidad da Coruña; EspañaFil: Capelli, Camilla. Institute Of Earth Sciences; SueciaFil: Cereijo, José. Universidad da Coruña; EspañaFil: Carballeira, Rafael. Universidad da Coruña; EspañaFil: Christensen, Søren T.. Cphbusiness Laboratory And Environment; DinamarcaFil: Christoffersen, Kirsten S.. Universidad de Copenhagen; DinamarcaFil: Clayer, Francois. Norwegian Institute For Water Research; NoruegaFil: De Eyto, Elvira. Marine Institute; MayotteFil: De Senerpont Domis, Lisette N.. Netherlands Institute of Ecology; Países BajosFil: Jordi Delgado, Martín. Universidad da Coruña; EspañaFil: Doubek, Jonathan P.. Lake Superior State University; Estados UnidosFil: Eaton, Ashley. University of Vermont; Estados UnidosFil: Erdogan, Seyda. Bozok University; TurquíaFil: Erina, Oxana. Lomonosov Moscow State University; RusiaFil: Ersoy, Zeynep. Universidade De Évora; PortugalFil: Feuchtmayr, Heidrun. No especifíca;Fil: Fugère, Vincent. Université du Québec a Montreal; CanadáFil: Galafassi, Silvia. Water Research Institute; ItaliaFil: Gonçalves, Vitor. Universidad de Porto. Facultad de Ciências. Centro de Investigação em Biodiversidade e Recursos Genéticos; PortugalFil: Grossart, Hans Peter. No especifíca;Fil: Hamilton, David. Griffith University; AustraliaFil: Hanson, Paul C.. University of Wisconsin; Estados UnidosFil: Harris, Ted. University of Kansas; Estados UnidosFil: Başaran Kankılıç, Gökben. Kırıkkale University; TurquíaFil: Kessler, Rebecca. University of Kansas; Estados UnidosFil: Jacquet, Stéphan. No especifíca;Fil: Kangur, Külli. Estonian University Of Life Sciences; EstoniaFil: Kiel, Christine. No especifíca;Fil: Knoll, Lesley. University of Minnesota; Estados UnidosFil: Kokorīte, Ilga. No especifíca;Fil: Lavoie, Isabelle. Institut National De La Recherche Scientifique; CanadáFil: Leiva Presa, Àngels. No especifíca;Fil: Lepori, Fabio. University Of Applied Sciences And Arts Of Southern Switzerland; SuizaFil: Lusher, Amy. Norwegian Institute For Water Research; NoruegaFil: Macintyre, Sally. University of California; Estados UnidosFil: Matias, Miguel. Universidade De Évora; PortugalFil: Matsuzaki, Shin Ichiro S.. National Institute For Enviromental Studies; JapónFil: Mccarthy, Valerie. Dundalk Institute Of Technology; IrlandaFil: Mcelarney, Yvonne. Agri - Food And Bioscience Institute; IrlandaFil: Mcnally, David. Winooski High School; Estados UnidosFil: Mekonen Belay, Berte. Netherlands Institute Of Ecology; Países BajosFil: Messyasz, Beata. Adam Mickiewicz University In Poznan; PoloniaFil: Mlambo, Musa. Rhodes University. Albany Museum.; SudáfricaFil: Nandini, Sarma. Universidad Nacional Autónoma de México; MéxicoFil: Nodine, Emily. Rollins College; Estados UnidosFil: Özen, Arda. Çankırı Karatekin University; TurquíaFil: Ozkundakci, Deniz. Waikato Regional Council Science; Nueva ZelandaFil: Vazquez Perez, Ricardo. Universidad da Coruña; EspañaFil: Pociecha, Agnieszka. Institute Of Nature Conservation; PoloniaFil: Raposeiro, Pedro. Universidad de Porto. Facultad de Ciências. Centro de Investigação em Biodiversidade e Recursos Genéticos; PortugalFil: Rõõm, Eva Ingrid. Estonian University Of Life Sciences; EstoniaFil: Salmaso, Nico. Research And Innovation Centre; ItaliaFil: Sarma, Singaraju S. S.. Facultad De Estudios Superiores Iztacala; MéxicoFil: Saulnier Talbot, Émilie. Laval University. Centre Hospitalier de L'universite Laval; CanadáFil: Scordo, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; ArgentinaFil: Sibomana, Claver. University Of Burundi; BurundiFil: Stepanowska, Katarzyna. West Pomeranian University Of Technology In Szczecin; PoloniaFil: Tavşanoğlu, Ü. Nihan. Çankırı Karatekin University; TurquíaFil: Tolotti, Monica. Research And Innovation Centre; ItaliaFil: Udoh, Abel. Gazi University; TurquíaFil: Urrutia Cordera, Pablo. Uppsala University; SueciaFil: Valois, Amanda. National Institute Of Water And Atmospheric Research; Nueva ZelandaFil: Vandergoes, Marcus. No especifíca;Fil: Verburg, Piet. National Institute Of Water And Atmospheric Research; Nueva ZelandaFil: Volta, Pietro. Water Research Institute - National Research Council; ItaliaFil: Wain, Danielle. No especifíca;Fil: Wesolek, Brian. Bay Mills Indian Community; Estados UnidosFil: Weyhenmeyer, Gesa. Uppsala University; SueciaFil: Wightman, Ryan. Agri - Food And Bioscience Institute; IrlandaFil: Wood, Susie. Cawthron Institute; Nueva ZelandaFil: Wu, Naicheng. Ningbo University; ChinaFil: Zawiska, Izabela. Polish Academy of Sciences; ArgentinaFil: Zawisza, Edyta. Polish Academy of Sciences; ArgentinaFil: Zink, Lauren. University Of Lethbridge; CanadáFil: Leoni, Barbara. University Of Milano Bicocca; ItaliaThe Global Lake Ecological Observatory Network All Hands' MeetingEstados UnidosGlobal Lake Ecological Observatory Networ