Pre-plant release enhanced the earlier establishment of Nesidiocoris tenuis in open field tomato

Ο εχθρός της τομάτας, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) που εισέβαλε στη χώρα μας, πολύ σύντομα έγινε σημαντικός εχθρός τόσο των υπαίθριων όσο και των θερμοκηπιακών καλλιεργειών. Η εξαπόλυση του αρπακτικού Nesidiocoris tenuis (Reuter) (Heteroptera: Miridae) στο φυτώριο έχει βρεθεί ότι μπορεί να συμβάλει στην αντιμετώπιση του T. absoluta σε καλλιέργειες τομάτας στο θερμοκήπιο. Ο στόχος της παρούσας μελέτης είναι να αξιολογηθεί κατά πόσο σε υπαίθρια καλλιέργεια τομάτας, η εξαπόλυση του N. Tenuis στο φυτώριο μπορεί να συμβάλει στην έγκαιρη εγκατάστασή του. Συμπερασματικά, η εξαπόλυση του N. tenuis συνέβαλε ουσιαστικά στην έγκαιρη αποίκηση της καλλιέργειας σε σύγκριση με τα τεμάχια του μάρτυρα. Επίσης, ο ρόλος του ιθαγενούς πληθυσμού του M. pygmaeus πρέπει να λαμβάνεται σοβαρά υπόψη στα προγράμματα ολοκληρωμένης αντιμετώπισης. Η μέθοδος αυτή είναι αποτελεσματική στην πιο έγκαιρη εγκατάσταση του N. tenuis και θα πρέπει να αξιολογηθεί περαιτέρω η συμβολή της στην αντιμετώπιση του Τ. absoluta.The invasive pest of the tomato crops, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), soon after its establishment became a major problem of outdoor and greenhouse tomato crops across the Mediterranean countries. The pre-plant release of the predator Nesidiocoris tenuis (Reuter) (Heteroptera: Miridae) has been found to substantially contribute to its establishment on the crop and efficient control of T. absoluta in greenhouses. The aim of the current study was to assess whether the pre-plant release of N. tenuis could contribute to its earlier establishment. It was concluded that the release of N. tenuis in the nursery contributed substantially in the earlier increase of its numbers in the field in comparison to the control plots. The native populations of M. pygmaeus appeared on the tomato plants before the native populations of N. tenuis. Thus, the conservation of both predators should be taken into consideration in the integrated management strategies against T. absoluta. Therefore, the application of this method in open field tomato crop enhances the earlier establishment of N. tenuis and should be further evaluated in the control of T. absoluta

Nonabsorbable Antibiotics Reduce Bacterial and Endotoxin Translocation in Hepatectomised Rats

There is increasing evidence that septic complications, occurring after major hepatectomies, may be caused by gram negative bacteria, translocating from the gut. We investigated in rats, the effect of extended hepatectomy on the structure and morphology of the intestinal mucosa as well as on the translocation of intestinal bacteria and endotoxins. We also examined the effect of nonabsorbable antibiotics on reducing the intestinal flora and consequently the phenomenon of translocation by administering neomycin sulphate and cefazoline. Hepatectomy was found to increase translocation, while administration of nonabsorbable antibiotics decreased it significantly. In addition, hepatectomy increased the aerobic cecal bacterial population, which normalised in the group receiving antibiotics. Among the histological parameters evaluated, villus height demonstrated a significant reduction after hepatectomy, while the number of villi per cm and the number of mitoses per crypt, remained unchanged. Our results indicate that administration of nonabsorbable antibiotics presents a positive effect on bacterial and endotoxin translocation after extended hepatectomy, and this may be related to reduction of colonic bacterial load as an intraluminal effect of antibiotics

Glucocorticoids regulate AKR1D1 activity in human liver in vitro and in vivo

Steroid 5β-reductase (AKR1D1) is highly expressed in human liver where it inactivates endogenous glucocorticoids and catalyses an important step in bile acid synthesis. Endogenous and synthetic glucocorticoids are potent regulators of metabolic phenotype and play a crucial role in hepatic glucose metabolism. However, the potential of synthetic glucocorticoids to be metabolised by AKR1D1 as well as to regulate its expression and activity has not been investigated. The impact of glucocorticoids on AKR1D1 activity was assessed in human liver HepG2 and Huh7 cells; AKR1D1 expression was assessed by qPCR and Western blotting. Genetic manipulation of AKR1D1 expression was conducted in HepG2 and Huh7 cells and metabolic assessments were made using qPCR. Urinary steroid metabolite profiling in healthy volunteers was performed pre- and post-dexamethasone treatment, using gas chromatography-mass spectrometry. AKR1D1 metabolised endogenous cortisol, but cleared prednisolone and dexamethasone less efficiently. In vitro and in vivo, dexamethasone decreased AKR1D1 expression and activity, further limiting glucocorticoid clearance and augmenting action. Dexamethasone enhanced gluconeogenic and glycogen synthesis gene expression in liver cell models and these changes were mirrored by genetic knockdown of AKR1D1 expression. The effects of AKR1D1 knockdown were mediated through multiple nuclear hormone receptors, including the glucocorticoid, pregnane X and farnesoid X receptors. Glucocorticoids down-regulate AKR1D1 expression and activity and thereby reduce glucocorticoid clearance. In addition, AKR1D1 down-regulation alters the activation of multiple nuclear hormone receptors to drive changes in gluconeogenic and glycogen synthesis gene expression profiles, which may exacerbate the adverse impact of exogenous glucocorticoids

Glucocorticoids regulate AKR1D1 activity in human liver in vitro and in vivo

Steroid 5β-reductase (AKR1D1) is highly expressed in human liver where it inactivates endogenous glucocorticoids and catalyses an important step in bile acid synthesis. Endogenous and synthetic glucocorticoids are potent regulators of metabolic phenotype and play a crucial role in hepatic glucose metabolism. However, the potential of synthetic glucocorticoids to be metabolised by AKR1D1 as well as to regulate its expression and activity has not been investigated. The impact of glucocorticoids on AKR1D1 activity was assessed in human liver HepG2 and Huh7 cells; AKR1D1 expression was assessed by qPCR and Western blotting. Genetic manipulation of AKR1D1 expression was conducted in HepG2 and Huh7 cells and metabolic assessments were made using qPCR. Urinary steroid metabolite profiling in healthy volunteers was performed pre- and post-dexamethasone treatment, using gas chromatography-mass spectrometry. AKR1D1 metabolised endogenous cortisol, but cleared prednisolone and dexamethasone less efficiently. In vitro and in vivo, dexamethasone decreased AKR1D1 expression and activity, further limiting glucocorticoid clearance and augmenting action. Dexamethasone enhanced gluconeogenic and glycogen synthesis gene expression in liver cell models and these changes were mirrored by genetic knockdown of AKR1D1 expression. The effects of AKR1D1 knockdown were mediated through multiple nuclear hormone receptors, including the glucocorticoid, pregnane X and farnesoid X receptors. Glucocorticoids down-regulate AKR1D1 expression and activity and thereby reduce glucocorticoid clearance. In addition, AKR1D1 down-regulation alters the activation of multiple nuclear hormone receptors to drive changes in gluconeogenic and glycogen synthesis gene expression profiles, which may exacerbate the adverse impact of exogenous glucocorticoids

Antimicrobial use in European acute care hospitals: results from the second point prevalence survey (PPS) of healthcare-associated infections and antimicrobial use, 2016 to 2017

Antimicrobial agents used to treat infections are life-saving. Overuse may result in more frequent adverse effects and emergence of multidrug-resistant microorganisms. In 2016-17, we performed the second point-prevalence survey (PPS) of healthcare-associated infections (HAIs) and antimicrobial use in European acute care hospitals. We included 1,209 hospitals and 310,755 patients in 28 of 31 European Union/European Economic Area (EU/EEA) countries. The weighted prevalence of antimicrobial use in the EU/EEA was 30.5% (95% CI: 29.2-31.9%). The most common indication for prescribing antimicrobials was treatment of a community-acquired infection, followed by treatment of HAI and surgical prophylaxis. Over half (54.2%) of antimicrobials for surgical prophylaxis were prescribed for more than 1 day. The most common infections treated by antimicrobials were respiratory tract infections and the most commonly prescribed antimicrobial agents were penicillins with beta-lactamase inhibitors. There was wide variation of patients on antimicrobials, in the selection of antimicrobial agents and in antimicrobial stewardship resources and activities across the participating countries. The results of the PPS provide detailed information on antimicrobial use in European acute care hospitals, enable comparisons between countries and hospitals, and highlight key areas for national and European action that will support efforts towards prudent use of antimicrobials

Social marketing and healthy eating : Findings from young people in Greece

This document is the Accepted Manuscript version. The final publication is available at Springer via http://dx.doi.org/10.1007/s12208-013-0112-xGreece has high rates of obesity and non-communicable diseases owing to poor dietary choices. This research provides lessons for social marketing to tackle the severe nutrition-related problems in this country by obtaining insight into the eating behaviour of young adults aged 18–23. Also, the main behavioural theories used to inform the research are critically discussed. The research was conducted in Athens. Nine focus groups with young adults from eight educational institutions were conducted and fifty-nine participants’ views towards eating habits, healthy eating and the factors that affect their food choices were explored. The study found that the participants adopted unhealthier nutritional habits after enrolment. Motivations for healthy eating were good health, appearance and psychological consequences, while barriers included lack of time, fast-food availability and taste, peer pressure, lack of knowledge and lack of family support. Participants reported lack of supportive environments when deciding on food choices. Based on the findings, recommendations about the development of the basic 4Ps of the marketing mix, as well as of a fifth P, for Policy are proposedPeer reviewe

The intonation and pragmatics of Greek wh-questions

We experimentally tested three hypotheses regarding the pragmatics of two tunes (one high-ending, one flat-ending) used with Greek wh-questions: (a) the high-ending tune is associated with information-seeking questions, while the flat-ending tune is also appropriate when wh-questions are not information-seeking in which case their function can instead be akin to that of a statement; (b) the high-ending tune is more polite, and (c) more appropriate for contexts leading to information-seeking questions. The wh-questions used as experimental stimuli were elicited from four speakers in contexts likely to lead to either information-seeking or non-information-seeking uses. The speakers produced distinct tunes in response to the contexts; acoustic analysis indicates these are best analysed as L*+H L-!H% (rising), and L+H* L-L% (flat). In a perception experiment where participants heard the questions out of context, they chose answers providing information significantly more frequently after high-ending than flat-ending questions, confirming hypothesis (a). In a second experiment testing hypotheses (b) and (c), participants evaluated wh-questions for appropriateness and politeness in information- and non-information-seeking contexts. High-ending questions were rated more appropriate in information-seeking contexts, and more polite independently of context relative to their flat-ending counterparts. Finally, two follow-up experiments showed that the interpretation of the two tunes was not affected by voice characteristics of individual speakers, and confirmed a participant preference for the high-ending tune. Overall, the results support our hypotheses and lead to a compositional analysis of the meaning of the two tunes, while also showing that intonational meaning is determined by both tune and pragmatic context

Hypoxia-induced long non-coding RNA Malat1 is dispensable for renal ischemia/reperfusion-injury

Renal ischemia-reperfusion (I/R) injury is a major cause of acute kidney injury (AKI). Non-coding RNAs are crucially involved in its pathophysiology. We identified hypoxia-induced long non-coding RNA Malat1 (Metastasis Associated Lung Adenocarcinoma Transcript 1) to be upregulated in renal I/R injury. We here elucidated the functional role of Malat1 in vitro and its potential contribution to kidney injury in vivo. Malat1 was upregulated in kidney biopsies and plasma of patients with AKI, in murine hypoxic kidney tissue as well as in cultured and ex vivo sorted hypoxic endothelial cells and tubular epithelial cells. Malat1 was transcriptionally activated by hypoxia-inducible factor 1-a. In vitro, Malat1 inhibition reduced proliferation and the number of endothelial cells in the S-phase of the cell cycle. In vivo, Malat1 knockout and wildtype mice showed similar degrees of outer medullary tubular epithelial injury, proliferation, capillary rarefaction, inflammation and fibrosis, survival and kidney function. Small-RNA sequencing and whole genome expression analysis revealed only minor changes between ischemic Malat1 knockout and wildtype mice. Contrary to previous studies, which suggested a prominent role of Malat1 in the induction of disease, we did not confirm an in vivo role of Malat1 concerning renal I/Rinjury