Detection of optical coronal emission from 10^6 K gas in the core of the Centaurus cluster

We report a detection (3.5x10^37 \pm 5.6x10^36 ergps) of the optical coronal emission line [Fe X]6374 and upper limits of four other coronal lines using high resolution VIMOS spectra centred on NGC 4696, the brightest cluster galaxy in the Centaurus cluster. Emission from these lines is indicative of gas at temperatures between 1 and 5 million K so traces the interstellar gas in NGC 4696. The rate of cooling derived from the upper limits is consistent with the cooling rate from X-ray observations (~10 solar masses per year) however we detect twice the luminosity expected for [Fe X]6374 emission, at 1 million K, our lowest temperature probe. We suggest this emission is due to the gas being heated rather than cooling out of the intracluster medium. We detect no coronal lines from [Ca XV], which are expected from the 5 million K gas seen near the centre in X-rays with Chandra. Calcium is however likely to be depleted from the gas phase onto dust grains in the central regions of NGC 4696.Comment: 11 pages, 13 figures, 2 tables, accepted for publication in MNRA

Rectal Transmission of Transmitted/Founder HIV-1 Is Efficiently Prevented by Topical 1% Tenofovir in BLT Humanized Mice

Rectal microbicides are being developed to prevent new HIV infections in both men and women. We focused our in vivo preclinical efficacy study on rectally-applied tenofovir. BLT humanized mice (n = 43) were rectally inoculated with either the primary isolate HIV-1(JRCSF) or the MSM-derived transmitted/founder (T/F) virus HIV-1(THRO) within 30 minutes following treatment with topical 1% tenofovir or vehicle. Under our experimental conditions, in the absence of drug treatment we observed 50% and 60% rectal transmission by HIV-1(JRCSF) and HIV-1(THRO), respectively. Topical tenofovir reduced rectal transmission to 8% (1/12; log rank p = 0.03) for HIV-1(JRCSF) and 0% (0/6; log rank p = 0.02) for HIV-1(THRO). This is the first demonstration that any human T/F HIV-1 rectally infects humanized mice and that transmission of the T/F virus can be efficiently blocked by rectally applied 1% tenofovir. These results obtained in BLT mice, along with recent ex vivo, Phase 1 trial and non-human primate reports, provide a critically important step forward in the development of tenofovir-based rectal microbicides

RNAcentral 2021: secondary structure integration, improved sequence search and new member databases

RNAcentral is a comprehensive database of non-coding RNA (ncRNA) sequences that provides a single access point to 44 RNA resources and >18 million ncRNA sequences from a wide range of organisms and RNA types. RNAcentral now also includes secondary (2D) structure information for >13 million sequences, making RNAcentral the world's largest RNA 2D structure database. The 2D diagrams are displayed using R2DT, a new 2D structure visualization method that uses consistent, reproducible and recognizable layouts for related RNAs. The sequence similarity search has been updated with a faster interface featuring facets for filtering search results by RNA type, organism, source database or any keyword. This sequence search tool is available as a reusable web component, and has been integrated into several RNAcentral member databases, including Rfam, miRBase and snoDB. To allow for a more fine-grained assignment of RNA types and subtypes, all RNAcentral sequences have been annotated with Sequence Ontology terms. The RNAcentral database continues to grow and provide a central data resource for the RNA community

RNAcentral 2021: secondary structure integration, improved sequence search and new member databases.

RNAcentral is a comprehensive database of non-coding RNA (ncRNA) sequences that provides a single access point to 44 RNA resources and >18 million ncRNA sequences from a wide range of organisms and RNA types. RNAcentral now also includes secondary (2D) structure information for >13 million sequences, making RNAcentral the world's largest RNA 2D structure database. The 2D diagrams are displayed using R2DT, a new 2D structure visualization method that uses consistent, reproducible and recognizable layouts for related RNAs. The sequence similarity search has been updated with a faster interface featuring facets for filtering search results by RNA type, organism, source database or any keyword. This sequence search tool is available as a reusable web component, and has been integrated into several RNAcentral member databases, including Rfam, miRBase and snoDB. To allow for a more fine-grained assignment of RNA types and subtypes, all RNAcentral sequences have been annotated with Sequence Ontology terms. The RNAcentral database continues to grow and provide a central data resource for the RNA community. RNAcentral is freely available at https://rnacentral.org

Syncytiotrophoblast derived extracellular vesicles transfer functional placental miRNAs to primary human endothelial cells

During the pregnancy associated syndrome preeclampsia (PE), there is increased release of placental syncytiotrophoblast extracellular vesicles (STBEVs) and free foetal haemoglobin (HbF) into the maternal circulation. In the present study we investigated the uptake of normal and PE STBEVs by primary human coronary artery endothelial cells (HCAEC) and the effects of free HbF on this uptake. Our results show internalization of STBEVs into primary HCAEC, and transfer of placenta specific miRNAs from STBEVs into the endoplasmic reticulum and mitochondria of these recipient cells. Further, the transferred miRNAs were functional, causing a down regulation of specific target genes, including the PE associated gene fms related tyrosine kinase 1 (FLT1). When co-treating normal STBEVs with HbF, the miRNA deposition is altered from the mitochondria to the ER and the cell membrane becomes ruffled, as was also seen with PE STBEVs. These findings suggest that STBEVs may cause endothelial damage and contribute to the endothelial dysfunction typical for PE. The miRNA mediated effects on gene expression may contribute to the oxidative and endoplasmic reticulum stress described in PE, as well as endothelial reprogramming that may underlay the increased risk of cardiovascular disease reported for women with PE later in life

A quasar-galaxy mixing diagram: quasar spectral energy distribution shapes in the optical to near-infrared

We define a quasar-galaxy mixing diagram using the slopes of their spectral energy distributions (SEDs) from 1 \u3bcm to 3000 \uc5 and from 1 to 3 \u3bcm in the rest frame. The mixing diagram can easily distinguish among quasar-dominated, galaxy-dominated and reddening-dominated SED shapes. By studying the position of the 413 XMM-selected type 1 AGN in the wide-field `Cosmic Evolution Survey' in the mixing diagram, we find that a combination of the Elvis et al. mean quasar SED with various contributions from galaxy emission and some dust reddening is remarkably effective in describing the SED shape from 0.3 to 3 \u3bcm for large ranges of redshift, luminosity, black hole mass and Eddington ratio of type 1 AGN. In particular, the location in the mixing diagram of the highest luminosity AGN is very close (within 1\u3c3) to that of the Elvis et al. SED template. The mixing diagram can also be used to estimate the host galaxy fraction and reddening in quasar. We also show examples of some outliers which might be AGN in different evolutionary stages compared to the majority of AGN in the quasar-host galaxy co-evolution cycle

Performance of the D-Egg Optical Sensor for the IceCube Upgrade

New optical sensors called the "D-Egg" have been developed for cost-effective instrumentation for the IceCube Upgrade. With two 8-inch high QE photomultipliers, they offer increased effective photocathode area while retaining as much of the successful IceCube Digital Optical Module (DOM) design as possible. Mass production of D-Eggs has started in 2020. By the end of 2021, there will be 310 D-Eggs produced with 288 deployed in the IceCube Upgrade. The D-Egg readout system uses advanced technologies in electronics and computing power. Each of the two PMT signals is digitized using ultra-low-power 14-bit ADCs with a sampling frequency of 250-MSPS, enabling seamless and lossless event recording from single-photon signals to signals exceeding 200pe within 10ns, as well as flexible event triggering. In this paper, we report the single photon detection performance as well as the multiple photon recording capability of D-Eggs from the mass production line which have been evaluated with the built-in DAQ system

A Search for Neutrinos from Decaying Dark Matter in Galaxy Clusters and Galaxies with IceCube

The observed dark matter abundance in the Universe can be explained with non-thermal, heavy dark matter models. In order for dark matter to still be present today, its lifetime has to far exceed the age of the Universe. In these scenarios, dark matter decay can produce highly energetic neutrinos, along with other Standard Model particles. To date, the IceCube Neutrino Observatory is the world’s largest neutrino telescope, located at the geographic South Pole. In 2013, the IceCube collaboration reported the first observation of high-energy astrophysical neutrinos. Since then, IceCube has collected a large amount of astrophysical neutrino data with energies up to tens of PeV, allowing us to probe the heavy dark matter models using neutrinos. We search the IceCube data for neutrinos from decaying dark matter in galaxy clusters and galaxies. The targeted dark matter masses range from 10 TeV to 10 PeV. In this contribution, we present the method and sensitivities of the analysis

Testing Hadronic Interaction Models with Cosmic Ray Measurements at the IceCube Neutrino Observatory

The IceCube Neutrino Observatory provides the opportunity to perform unique measurements of cosmic-ray air showers with its combination of a surface array and a deep detector. Electromagnetic particles and low-energy muons (∼GeV) are detected by IceTop, while a bundle of high-energy muons (>~400 GeV) can be measured in coincidence in IceCube. Predictions of air-shower observables based on simulations show a strong dependence on the choice of the high-energy hadronic interaction model. By reconstructing different composition-dependent observables, one can provide strong tests of hadronic interaction models, as these measurements should be consistent with one another. In this work, we present an analysis of air-shower data between 2.5 and 80 PeV, comparing the composition interpretation of measurements of the surface muon density, the slope of the IceTop lateral distribution function, and the energy loss of the muon bundle, using the models Sibyll 2.1, QGSJet-II.04 and EPOS-LHC. We observe inconsistencies in all models under consideration, suggesting they do not give an adequate description of experimental data. The results furthermore imply a significant uncertainty in the determination of the cosmic-ray mass composition through indirect measurements