20 research outputs found
Contaminación por Coliformes Totales y Escherichia Coli en Concha (Anadara Tuberculosa y Anadara Similis) en Jambelí, El Oro, Ecuador
This research directs the total microbiological coliform and Escherichia coli analysis in Anadara tubercullosa and Anadara similis; this research was done in the Archipelago of Jambeli, Province of El Oro. In the development of this study, samples were obtained from four strategic points. These samples were taken in the dry season (November) and in the rainy season (December); Chromocult Agar was used as a culture medium in 240 shells. The results obtained in the culture analysis show total coliform in A. similis. The median for the first sampling was 1.5x10-4 CFU/100g; the second sampling median was 2.0x10-4UFC/100g. For A. tuberculose the first sampling median was 1.7x10-4 CFUs/100g; the second sampling median was 2.4x10-4 CFU/100g. Among the four sampling points, point two was the one with the highest contamination; it had 2.1 x 10.4UFC/100g. The presence of E. coli was not found in either species. It was suggested that the species´ bacterial count level be reduced by means of a purifications system and by cooking the bivalves before eating them. Esta investigación dirige el análisis microbiológico de coliformes totales y Escherichia coli en Anadara Tuberculosa y Anadara Similis en el Archipiélago de Jambelí en la provincia de El Oro en Ecuador. Se obtuvieron muestras de cuatro puntos estratégicos en época seca (noviembre) y lluviosa (diciembre) usando el medio de cultivo Agar Chromocult en 240 conchas. En los resultados obtenidos en los cultivos de análisis, se presenció coliformes totales en Anadora Similis, con una media para el primer muestreo de 1,5x10^4 UFC/100g y segundo muestreo de 2.0x10^4UFC/100g y para Anadora Tuberculosa 1,7x10^4 UFC/100g y 2,4x10^4 UFC/100g. Entre los cuatro puntos de muestreo, el más elevado por contaminación es el punto dos con 2,1 x 10^4UFC/100g, en ambas especies no se observó presencia de Escherichia Coli. Se planteó la reducción del nivel bacteriano en las especies mediante un sistema de depuración y llevar a cocción los bivalvos antes de ingerirlos
Strong-Lensing Analysis of a Complete Sample of 12 MACS Clusters at z>0.5: Mass Models and Einstein Radii
We present the results of a strong-lensing analysis of a complete sample of
12 very luminous X-ray clusters at using HST/ACS images. Our modelling
technique has uncovered some of the largest known critical curves outlined by
many accurately-predicted sets of multiple images. The distribution of Einstein
radii has a median value of \simeq28\arcsec (for a source redshift of
), twice as large as other lower- samples, and extends to
55\arcsec for MACS J0717.5+3745, with an impressive enclosed Einstein mass of
. We find that 9 clusters cover a very large area
(>2.5 \sq \arcmin) of high magnification () for a source
redshift of , providing primary targets for accessing the first
stars and galaxies. We compare our results with theoretical predictions of the
standard CDM model which we show systematically fall short of our
measured Einstein radii by a factor of , after accounting for the
effect of lensing projection. Nevertheless, a revised analysis once arc
redshifts become available, and similar analyses of larger samples, are needed
in order to establish more precisely the level of discrepancy with CDM
predictions.Comment: Accepted for publication in MNRAS, 19 pages, 35 figures, 2 tables. V2
includes several changes, mainly additional discussion of the results. A
higher resolution version is available at
ftp://wise-ftp.tau.ac.il/pub/adiz/macs1
ImageCLEF 2019: Multimedia Retrieval in Lifelogging, Medical, Nature, and Security Applications
This paper presents an overview of the foreseen ImageCLEF 2019 lab that will be organized as part of the Conference and Labs of the Evaluation Forum - CLEF Labs 2019. ImageCLEF is an ongoing evaluation initiative (started in 2003) that promotes the evaluation of technologies for annotation, indexing and retrieval of visual data with the aim of providing information access to large collections of images in various usage scenarios and domains. In 2019, the 17th edition of ImageCLEF will run four main tasks: (i) a Lifelog task (videos, images and other sources) about daily activities understanding, retrieval and summarization, (ii) a Medical task that groups three previous tasks (caption analysis, tuberculosis prediction, and medical visual question answering) with newer data, (iii) a new Coral task about segmenting and labeling collections of coral images for 3D modeling, and (iv) a new Security
task addressing the problems of automatically identifying forged content and retrieve hidden information. The strong participation, with over 100 research groups registering and 31 submitting results for the tasks in 2018 shows an important interest in this benchmarking campaign and we expect the new tasks to attract at least as many researchers for 2019
Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments
[EN] Deep investigation of the microbiome of food-production and foodprocessing environments through whole-metagenome sequencing (WMS) can provide detailed information on the taxonomic composition and functional potential of the microbial communities that inhabit them, with huge potential benefits for environmental monitoring programs. However, certain technical challenges jeopardize the application of WMS technologies with this aim, with the most relevant one being the recovery of a sufficient amount of DNA from the frequently low-biomass samples collected from the equipment, tools and surfaces of food-processing plants. Here, we present the first complete workflow, with optimized DNA-purification methodology, to obtain high-quality WMS sequencing results from samples taken from food-production and food-processing environments and reconstruct metagenome assembled genomes (MAGs). The protocol can yield DNA loads >10 ng in >98% of samples and >500 ng in 57.1% of samples and allows the collection of, on average, 12.2 MAGs per sample (with up to 62 MAGs in a single sample) in ~1 week, including both laboratory and computational work. This markedly improves on results previously obtained in studies performing WMS of processing environments and using other protocols not specifically developed to sequence these types of sample, in which <2 MAGs per sample were obtained. The full protocol has been developed and applied in the framework of the European Union project MASTER (Microbiome applications for sustainable food systems through technologies and enterprise) in 114 food-processing facilities from different production sectors.SIThis work was funded by the European Commission under the European Union’s Horizon 2020 research and innovation program under grant agreement no. 818368 (MASTER). C.B. is grateful to Junta de Castilla y León and the European Social Fund for awarding her a pre-doctoral grant (BOCYL-D-07072020-6). A.P. is grateful to Ministerio de Ciencia e Innovación for awarding her a pre-doctoral grant (PRE2021-098910). N.M.Q. is currently funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 101034371. We thank AV Star Systems for their role in creating the Supplementary Video, and M. Coakley and S. Mortensen for their help in its preparation
ImageCLEF 2019: Multimedia Retrieval in Medicine, Lifelogging, Security and Nature
This paper presents an overview of the ImageCLEF 2019 lab, organized as part of the Conference and Labs of the Evaluation Forum - CLEF Labs 2019. ImageCLEF is an ongoing evaluation initiative (started in 2003) that promotes the evaluation of technologies for annotation, indexing and retrieval of visual data with the aim of providing information access to large collections of images in various usage scenarios and domains. In 2019, the 17th edition of ImageCLEF runs four main tasks: (i) a medical task that groups three previous tasks (caption analysis, tuberculosis prediction, and medical visual question answering) with new data, (ii) a lifelog task (videos, images and other sources) about daily activities understanding, retrieval and summarization, (iii) a new security task addressing the problems of automatically identifying forged content and retrieve hidden information, and (iv) a new coral task about segmenting and labeling collections of coral images for 3D modeling. The strong participation, with 235 research groups registering, and 63 submitting over 359 runs, shows an important interest in this benchmark campaign
Jorge Semprún entre sentiment et écriture
1. De l’engagement à l’écriture L’itinéraire de vie de Jorge Semprún ne peut être conçu sans l’engagement politique de sa personne et son engagement social en tant que citoyen. A côté de cela, il y a de fait, chez lui, un engagement linguistique qui jusqu’à aujourd’hui a tourné clairement en faveur de l’une des deux langues qui sont à la base de son éducation et de sa formation : le français et l’espagnol. Jusqu’à présent, la partie a été gagnée par le français, car selon les multiples vies q..
Experiencia y crítica a la educación religiosa en los escritores del 98 y posteriores
Los escritores pertenecientes a la llamada Generación del 98, conscientes de una necesidad de un cambio de rumbo y de una renovación de la vida española, son los primeros en criticar, de una manera abierta y profunda, la política llevada a cabo por los dirigentes españoles a finales del siglo XIX. La iglesia, que jugaba un papel importante y decisivo, era la responsable de la educación de estas clases dirigentes. Y responsable, también, de que las ideas que ella impartía a sus hijos, fueran s..
Diverging cell wall strategies for drought adaptation in two maize inbreds with contrasting lodging resistance
The plant cell wall is a plastic structure of variable composition that constitutes the first line of defence against environmental challenges. Lodging and drought are two stressful conditions that severely impact maize yield. In a previous work, we characterised the cell walls of two maize inbreds, EA2024 (susceptible) and B73 (resistant) to stalk lodging. Here, we show that drought induces distinct phenotypical, physiological, cell wall, and transcriptional changes in the two inbreds, with B73 exhibiting lower tolerance to this stress than EA2024. In control conditions, EA2024 stalks had higher levels of cellulose, uronic acids and p‐coumarate than B73.
However, upon drought EA2024 displayed increased levels of arabinose‐enriched polymers, such as pectin‐arabinans and arabinogalactan proteins, and a decreased lignin content. By contrast, B73 displayed a deeper rearrangement of cell walls upon drought, including modifications in lignin composition (increased S subunits and S/G ratio; decreased H subunits) and an increase of uronic acids. Drought induced more substantial changes in gene expression in B73 compared to EA2024, particularly in cell wall‐related genes, that were modulated in an inbred‐specific manner. Transcription factor enrichment assays unveiled inbred‐specific regulatory networks coordinating cell wall genes expression. Altogether, these findings reveal that B73 and EA2024 inbreds, with opposite stalk‐lodging phenotypes, undertake different cell wall modification strategies in response to drought. We propose that the specific cell wall composition conferring lodging resistance to B73, compromises its cell wall
plasticity, and renders this inbred more susceptible to drought.This work was supported by the Grants AGL2014-58126-R; RTC-2016-5816-2 and PID2022-142786NB-I00 funded by MCIN/AEI/10.13039/501100011033 and ERDF “A way to make Europe” and received financial support from the CONSOLIDER-INGENIO programme (CSD2007-00036) from the Spanish Ministerio de Ciencia e Innovación. S.C. was financed with a PhD contract (PRE2019-089329) funded by MCIN/AEI/10.13039/501100011033 and by “ESF Investing in your future”, and by SEV-2015-0533-19-1 and CEX2019-000902-S funded by MCIN/AEI/10.13039/501100011033. A.M.-R. was financed with a PhD contract from the Consejería de Educación de Castilla y León and the Fondo Social Europeo (ORDEN EDU/601/2020, July 7th). This work was also supported by the SGR programmes (2017SGR710 and 2021-SGR-01131) from the Secretaria d'Universitats i Recerca del Departament d'Empresa i Coneixement de la Generalitat de Catalunya and by the CERCA Programme/Generalitat de Catalunya. This work was financially supported by RYC2021-033414-l to R.U funded by MCIN/AEI/10.13039/501100011033 by the “European Union Next Generation EU/PRTR”. Finally, we acknowledge financial support from the Grants SEV-2015-0533-19-1 and CEX2019-000902-S funded by MCIN/AEI/10.13039/501100011033.info:eu-repo/semantics/publishedVersio
Challenges - real time nano characterization related technlogieeS
The project CHALLENGES – Real-time nano-CHAracterization reLatEd techNoloGiEeS – aims to develop innovative Non-Destructive Techniques (NDTs) for reliable inline multiscale measurements down to the nanoscale, and fully compatible with different factory environments. The developed metrology technologies will enable the increase of speed, resolution, sensitivity, spectral range and compatibility within different nano-related production environments, finally improving products performance, quality and reliability, with the consequent bosting of competitiveness. The CHALLENGES’s innovation will be developed exploiting the plasmonic enhancement of optical signals. It will provide a non-destructive approach based on the use of multipurpose nano-optical techniques to enable a reliable real-time nano-scale characterization in the factory floor, using plasmonic enhanced Raman, InfraRed (IR) and Photoluminescence signals.
CHALLENGES is focused on broadening the scope of AFM techniques useable in semiconductor manufacturing by implementing suitable plasmonic-based technologies to bring in industrial environments the capabilities of optical spectroscopies at the nanoscale, already demonstrated at lab level. Such composite and hybrid measurement techniques will enable new applications for the already proven industrial AFM-based characterization technologies. Signal amplification by localized plasmon resonance at a sharp AFM tip will allow improving both the spatial resolution well beyond the optical diffraction limit and the local signal intensity with an improvement of the signal/noise ratio.
Improvements are also expected concerning the time scale resolving capabilities. The improvement of the spatial resolution will allow to obtain nanoscale spectral maps, compatible with the size of the current electronic devices, while the improved signal-to-noise ratio will allow for a faster and reliable punctual analysis.
The final goal is to develop nanoscale metrological NDTs based on SPM platforms, for doping, annealing, metal contamination, dangling bonds presence and strain measurement directly within the production lines with real-time capabilities
Diatomitas de la provincia de Albacete
Las primeras referencias a la utilización de diatomitas como rocas industriales se trasladan a la antigua Grecia utilizadas en construcción, como aligerantes, en la fabricación de cerámica estructural y abrasivos. El nombre de diatomita viene del griego diatome que significa cortado por la mitad, este nombre hace referencia a la simetría radial de uno de los grandes grupos de diatomeas. En la antigua Roma la diatomita siguió utilizándose en construcción. Un ejemplo de ello es la iglesia de Santa Sofía en Constantinopla (535 a.C.) que posee un domo de unos 30 metros de diámetro construido con ladrillos de diatomitas (Marurrasse, 1978). No obstante, el éxito de la industria de la diatomita comenzó en el s. XIX con la invención de la dinamita por Alfred Nobel en 1867. Los explosivos de dinamita y de nitroglicerina necesitan grandes cantidades de diatomita que actúa como absorbente y estabilizador. A principios del s. XX se descubrieron otros usos para la diatomita. Dentro de estas primeras aplicaciones son de destacar las de aligerante en construcción, componentes de pulido, filtros y agente de carga. En los años 20 se desarrollaron las técnicas de procesado diversificando el mercado de la diatomita. Durante todo el s. XX se han sucedido repuntes en la producción y consumo de diatomitas relacionados con los principales conflictos bélicos. En este trabajo se incluyen la descripción estructural y mineralógica de las diatomitas, analizando las propiedades que le confieren a estas rocas un interés industrial. Asimismo, se consideran los principales campos de aplicación desde un punto de vista técnico y económico. Se describen los ambientes de formación más significativos haciendo hincapié en los depósitos españoles de la provincia de Albacete. Se detallan los métodos de extracción y procesado mineral previos a la comercialización del producto. En último término, se analizan las condiciones económicas del mercado de las diatomitas prestando especial interés a la actual situación de crisis económica mundial