Ozonized oils: a review of its quality control, stability and effectiveness in the treatment of Acne vulgaris

Acne affects most young people and its topical treatment with antibacterials is associated with increased bacterial resistance to antibiotics and adverse effects. As an alternative, ozone therapy stands out through the application of ozonized oils. The objective of this work was to raise the scientific evidence about the effectiveness in the treatment of acne, in addition to the techniques of characterization and stability of ozonated oils. This is an exploratory, descriptive study with a quantitative approach, based on the analysis of scientific references in a bibliographic review of the expository type, of the last 20 years. Among the selected references, only four manuscripts reporting clinical studies of ozone therapy, with controversial results. Seven articles with the physicochemical characterization of ozonated oils were found. The major part of manuscripts reported the use of sunflower, sesame and olive oil. The more common techniques used to characterize the ozonation process are the peroxide value (PV) and the iodine index (Ii), which represents the proportion of unsaturated groups, whose values increase and decrease, respectively with ozonization progress. The viscosity of oils is increased by the formation of polymeric peroxides; the FTIR spectrum, which identifies the decrease in the stretch bands C = C, in addition to ozone formation, monitored by NMR, are also employed. Increased antimicrobial activity has been demonstrated with the ozone level of the oils, but the activity against Cutibacterium acne has not been reported. Only two article reported satisfactory stability for 6 months of refrigerated ozonized oil or kept at room temperature, showing the need for more specific research to support the application of ozonized oils in the treatment of acne and stability data of these products

Bactericidal action of ABO isoagglutinins at different dilutions of anti-ABO antibodies

Tendo em vista o grande polimorfismo dos sistemas de grupos sanguíneos e que uma das hipóteses para explicá-los seja a co-evolução com patógenos, esta pesquisa teve como objetivo avaliar a ação bactericida in vitro dos anticorpos anti-ABO sobre a bactéria Escherichia coli. Foi observada diferença significativa no crescimento bacteriano (p<0,0001) nas comparações do Grupo O, Grupo A, Grupo B e Grupo AB, quando comparados ao controle positivo, demonstrando o efeito bactericida dos anticorpos anti-ABO. Não houve diferença significativa (p > 0,05) intergrupos sanguíneos O, A, B e AB. Apesar de não haver diferença estatística (p > 0,05) intergrupos sanguíneos O, A, B e AB, o grupo sanguíneo AB apresentou menor ação bactericida. A partir da ação bactericida dos anticorpos anti-ABO, estes podem ser utilizados no futuro como alternativas não farmacológicas no auxílio de quadros clínicos de sepse.In view of the big polymorphism of the blood group systems and that the hypothesis to explain them is the co-evolution with pathogens, this research had as its objective to evaluate the bactericide action in vitro of the anti-ABO antibodies upon the Escherichia coli bacteria. It was observed a substantial difference in the bacterial growth (p<0,0001) in the comparisons of Group O, Group A, Group B, and Group AB when compared to the Positive Control, demonstrating the bactericide effect of the anti-ABO antibodies. There was no significant difference (p > 0,05) between the blood systems O, A, B, and AB. Although there was no statistical difference (p > 0.05) between blood groups O, A, B and AB, blood group AB had less bactericidal action. Based on the bactericidal action of anti-ABO antibodies, these can be used in the future as non-pharmacological alternatives to help with sepsis clinical conditions

Seasonal phytochemical study and antimicrobial potential of Vetiveria zizanioides roots

This paper describes the seasonal phytochemical variation and the antimicrobial potential of V. zizanioides roots collected in Brazil. Considering the high levels of chemical constituents and their biological activity in dichloromethane fraction, the plants were grown in different seasons and the respective dichloromethane fractions were analyzed by gas chromatography-mass spectrometry. The antimicrobial activity was evaluated against several pathogenic microorganisms by determining the minimum inhibitory concentration (MIC) by the agar dilution method. The yields of dichloromethane fractions from plants collected in the autumn and spring occurred in a higher proportion than in other seasons. Khusimol (2) was isolated by column chromatography and identified by NMR and CG-MS, along with other sesquiterpenes, including β-vetivenene (1), vetiselinenol (3), isovalencenol (4), vetivenic acid (5), α-vetivone (6) and β-vetivone (7). Some extracts showed promising antimicrobial effects, with MICs ranging from 31.25 to 500 μg mL–1. Kushimol was slightly active against the tested microorganisms

Composição química e avaliação da atividade antimicrobiana do óleo essencial das folhas de Piper malacophyllum (C. Presl.) C. DC.

This work reports the chemical composition as well as the antibacterial, antifungal and antiparasitic activities of the leaf essential oil from Piper malacophyllum. The oil was extracted by hydrodistillation and analyzed by GC-FID, GC-MS and polarimetry. Among the 28 compounds identified, (+)-camphor was the major constituent. The essential oil showed activity against most of the microorganisms tested, especially antifungal action, with a MIC of 500 µg mL-1 against Trichophyton mentagrophytes and Cryptococcus neoformans. This is the first study reporting the composition and biological properties of leaf essential oil from P. malacophyllum

GASTRO PROTECTIVE AND ANTI-HELICOBACTER PYLORI EFFECTS OF A FLAVONOID RICH FRACTION OBTAINED FROM ACHYROCLINE SATUREOIDES (LAM) D.C.

Objectives: The study aimed to investigate the gastroprotective effects of a flavonoid rich fraction (FRF) obtained from Achyrocline satureoides. Methods: The following protocols were employed: ethanol and NSAID-induced ulcer, ligature pylorus model, and free mucus quantification. Nitric oxide (NO) and sulfhydryl group participation were observed by pretreatment with L-NAME or NEM. Besides, it was assayed the acetic acid-induced chronic ulcer andthe anti-Helicobacter pyloriactivity in vitro. Results: The phytochemical profile of FRF showed three main flavonoids, luteolin, quercetin and 3-O-methyl-quercetin. The administration of FRF was able to prevent the damage evoked by ethanol and NSAID-induced ulcer models. The pH and concentration of H+ in the stomach were not modified by FRF treatment. However, the FRF treatment induces mucus secretion. The effect presented by FRF was mediated by nitric oxide (NO). In chronic ulcer model FRF reduced significantly the lesion area, promoting a cure ratio of 65.42±13.00, a similar data presented by cimetidine treated animals (61.35±11.88). Using an in vitro assay was observed that FRF at 500 µg/mL was able to inhibit bacterial growth. Conclusions: The results show that FRF provided a significant gastroprotective and ulcer healing activity, mainly due to their capacity to enhance mucus secretion

Antibacterial activity and toxicity of drimys brasiliensis

Men have used medicinal plant properties to treat infectious diseases. Both the rise of emerging infectious diseases as the microbial resistance problem has stimulated the searching for new antimicrobial agents. This study evaluated the antibacterial activity and toxicity of crude extracts, fractions and pure compounds from Drimys brasiliensis. The antibacterial activity of five extracts, twelve fractions and five isolated compounds were tested against six Gram-positive and seven Gram-negative bacteria. The methodology used was agar dilution. The extract potential toxicities were evaluated using Artemia salina assay. Antibacterial activity tests showed some promising results, such as bark chloroform extract with minimum inhibitory concentration (MIC) of 62.5 μg/mL for Bacillus cereus, fraction G2 with MIC for Staphylococcus aureus of 62.5 μg/mL, and methoxy-polygodial compound with MIC to Bacillus cereus of 31.25 μg/mL. There was no activity against Gram-negative bacteria. The bark dichloromethane extract showed MIC of 1000 μg/mL against Helicobacter pylori. The best results corresponded to fractions E and G2, with a MIC of 500 μg/mL. Among the isolated compounds, polygodial showed better activity with MIC of 250 μg/mL. Artemia salina tests showed that the bark dichloromethane extract and the fractions E and G2 showed toxicity, with LC50 values of 27.51, 25.29 and 139.7 μg/mL, respectively. The results showed the antibacterial activity of Drimys brasiliensis, with potential toxicity, but with possible antimutagenic action.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Measurement of the inclusive and dijet cross-sections of b-jets in pp collisions at sqrt(s) = 7 TeV with the ATLAS detector

The inclusive and dijet production cross-sections have been measured for jets containing b-hadrons (b-jets) in proton-proton collisions at a centre-of-mass energy of sqrt(s) = 7 TeV, using the ATLAS detector at the LHC. The measurements use data corresponding to an integrated luminosity of 34 pb^-1. The b-jets are identified using either a lifetime-based method, where secondary decay vertices of b-hadrons in jets are reconstructed using information from the tracking detectors, or a muon-based method where the presence of a muon is used to identify semileptonic decays of b-hadrons inside jets. The inclusive b-jet cross-section is measured as a function of transverse momentum in the range 20 < pT < 400 GeV and rapidity in the range |y| < 2.1. The bbbar-dijet cross-section is measured as a function of the dijet invariant mass in the range 110 < m_jj < 760 GeV, the azimuthal angle difference between the two jets and the angular variable chi in two dijet mass regions. The results are compared with next-to-leading-order QCD predictions. Good agreement is observed between the measured cross-sections and the predictions obtained using POWHEG + Pythia. MC@NLO + Herwig shows good agreement with the measured bbbar-dijet cross-section. However, it does not reproduce the measured inclusive cross-section well, particularly for central b-jets with large transverse momenta.Comment: 10 pages plus author list (21 pages total), 8 figures, 1 table, final version published in European Physical Journal