COVID-19 information disorder:six types of harmful information during the pandemic in Europe

{Sten Hansson and Kati Orru and Sten Torpan and Asta Bäck and Austeja Kazemekaityte and Sunniva Frislid Meyer and Johanna Ludvigsen and Lucia Savadori and Alessandro Galvagni and Ala Pigrée}, {COVID-19 information disorder: six types of harmful information during the pandemic in Europe}, {Journal of Risk Research}, {24}, {3-4}, {380-393}, {2021}, {Routledge}, {10.1080/13669877.2020.1871058}, { https://doi.org/10.1080/13669877.2020.1871058}The outbreak of a novel coronavirus disease COVID-19 propelled the creation, transmission, and consumption of false information – unverified claims, misleading statements, false rumours, conspiracy theories, and so on – all around the world. When various official or unofficial sources issue erroneous, misleading or contradicting information during a crisis, people who are exposed to this may behave in ways that cause harm to the health and well-being of themselves or others, e.g., by not taking appropriate risk reducing measures or blaming or harassing vulnerable groups. To work towards a typology of informational content that may increase people’s vulnerability in the context of the coronavirus pandemic, we explored 98 instances of potentially harmful information that spread in six European countries – France, Italy, Norway, Finland, Lithuania, and Estonia – between March and May 2020. We suggest that during the pandemic, exposure to harmful information may have made people more vulnerable in six ways: (1) by discouraging appropriate protective actions against catching/spreading the virus, (2) by promoting the use of false (or harmful) remedies against the virus, (3) by misrepresenting the transmission mechanisms of the virus, (4) by downplaying the risks related to the pandemic, (5) by tricking people into buying fake protection against the virus or into revealing their confidential information, and (6) by victimising the alleged spreaders of the virus by harassment/hate speech. The proposed typology can be used to guide the development of risk communication plans to address each of these information-related vulnerabilities.publishedVersio

Life satisfaction during temporary housing after an earthquake: Comparing three cases in Italy

A disaster has a considerable impact on people who have lost their homes. We analyzed the life satisfaction of displaced people accommodated in temporary housing after three strong earthquakes that occurred in Italy in 2009 (Abruzzo), 2012 (Emilia), and 2016–17 (Central Italy). Information was obtained through an anonymous survey on a large number of variables related to socio-demographic features, temporary housing type and quality, social support and protection network, personal psychological resources and preparedness, and psychophysical health. Responses were collected through face-to-face and telephone interviews, stand-alone paper-and-pencil questionnaires, or online (N = 261). In the whole-sample analysis, the significant predictors of life satisfaction were earthquake preparedness, which earthquake out of three was experienced, protection network, accommodation type and quality, and general health status, thus highlighting the complex and multi-faceted nature of the underpinnings of life satisfaction in people displaced after an earthquake. However, different predictors explained life satisfaction in the analysis of each earthquake, pointing to the need to consider their specificity and the local context. Nevertheless, the perceived quality of the accommodation was a significant predictor both in the whole-sample analysis and in the analysis of two earthquakes (2009 Abruzzo and 2012 Emilia), highlighting the importance of providing temporary houses with appropriate properties (privacy, space, thermal and acoustic insulation, light, quality of materials, surroundings) and placed in locations that allow the resumption of life activities (e.g., education, work, socialization, health and public facilities and services)

The collapse of protoplanetary clumps formed through disc instability: 3D simulations of the pre-dissociation phase

We present 3D smoothed particle hydrodynamics simulations of the collapse of clumps formed through gravitational instability in the outer part of a protoplanetary disc. The initial conditions are taken directly from a global disc simulation, and a realistic equation of state is used to follow the clumps as they contract over several orders of magnitude in density, approaching the molecular hydrogen dissociation stage. The effects of clump rotation, asymmetries, and radiative cooling are studied. Rotation provides support against fast collapse, but non-axisymmetric modes develop and efficiently transport angular momentum outward, forming a circumplanetary disc. This transport helps the clump reach the dynamical collapse phase, resulting from molecular hydrogen dissociation, on a thousand-year timescale, which is smaller than timescales predicted by some previous spherical 1D collapse models. Extrapolation to the threshold of the runaway hydrogen dissociation indicates that the collapse timescales can be shorter than inward migration timescales, suggesting that clumps could survive tidal disruption and deliver a proto-gas giant to distances of even a few AU from the central star.Comment: Accepted for publication in MNRA

Muscle pain in mitochondrial diseases: a picture from the Italian network

Muscle pain may be part of many neuromuscular disorders including myopathies, peripheral neuropathies and lower motor neuron diseases. Although it has been reported also in mitochondrial diseases (MD), no extensive studies in this group of diseases have been performed so far. We reviewed clinical data from 1398 patients affected with mitochondrial diseases listed in the database of the "Nation-wide Italian Collaborative Network of Mitochondrial Diseases", to assess muscle pain and its features. Muscle pain was present in 164 patients (11.7%). It was commonly observed in subjects with chronic progressive external ophthalmoplegia (cPEO) and with primary myopathy without cPEO, but also-although less frequently-in multisystem phenotypes such as MELAS, MERFF, Kearns Sayre syndrome, NARP, MNGIE and Leigh syndrome. Patients mainly complain of diffuse exercise-related muscle pain, but focal/multifocal and at rest myalgia were often also reported. Muscle pain was more commonly detected in patients with mitochondrial DNA mutations (67.8%) than with nuclear DNA changes (32.2%). Only 34% of the patients showed a good response to drug therapy. Interestingly, patients with nuclear DNA mutations tend to have a better therapeutic response than patients with mtDNA mutations. Muscle pain is present in a significant number of patients with MD, being one of the most common symptoms. Although patients with a myopathic phenotype are more prone to develop muscle pain, this is also observed in patients with a multi system involvement, representing an important and disabling symptom having poor response to current therapy

Sp1 Is Essential for p16(INK4a) Expression in Human Diploid Fibroblasts during Senescence

BACKGROUND: p16 (INK4a) tumor suppressor protein has been widely proposed to mediate entrance of the cells into the senescent stage. Promoter of p16 (INK4a) gene contains at least five putative GC boxes, named GC-I to V, respectively. Our previous data showed that a potential Sp1 binding site, within the promoter region from −466 to −451, acts as a positive transcription regulatory element. These results led us to examine how Sp1 and/or Sp3 act on these GC boxes during aging in cultured human diploid fibroblasts. METHODOLOGY/PRINCIPAL FINDINGS: Mutagenesis studies revealed that GC-I, II and IV, especially GC-II, are essential for p16 (INK4a) gene expression in senescent cells. Electrophoretic mobility shift assays (EMSA) and ChIP assays demonstrated that both Sp1 and Sp3 bind to these elements and the binding activity is enhanced in senescent cells. Ectopic overexpression of Sp1, but not Sp3, induced the transcription of p16 (INK4a). Both Sp1 RNAi and Mithramycin, a DNA intercalating agent that interferes with Sp1 and Sp3 binding activities, reduced p16 (INK4a) gene expression. In addition, the enhanced binding of Sp1 to p16 (INK4a) promoter during cellular senescence appeared to be the result of increased Sp1 binding affinity, not an alteration in Sp1 protein level. CONCLUSIONS/SIGNIFICANCE: All these results suggest that GC- II is the key site for Sp1 binding and increase of Sp1 binding activity rather than protein levels contributes to the induction of p16 (INK4a) expression during cell aging

Translational Regulation of Utrophin by miRNAs

Background Utrophin is the autosomal homolog of dystrophin, the product of the Duchenne Muscular Dystrophy (DMD) locus. Its regulation is of therapeutic interest as its overexpression can compensate for dystrophin's absence in animal models of DMD. The tissue distribution and transcriptional regulation of utrophin have been characterized extensively, and more recently translational control mechanisms that may underlie its complex expression patterns have begun to be identified. Methodology/Principal Findings Using a variety of bioinformatic, molecular and cell biology techniques, we show that the muscle isoform utrophin-A is predominantly suppressed at the translational level in C2C12 myoblasts. The extent of translational inhibition is estimated to be ~99% in C2C12 cells and is mediated by both the 5′- and 3′-UTRs of the utrophin-A mRNA. In this study we identify five miRNAs (let-7c, miR-150, miR-196b, miR-296-5p, miR-133b) that mediate the repression, and confirm repression by the previously identified miR-206. We demonstrate that this translational repression can be overcome by blocking the actions of miRNAs, resulting in an increased level of utrophin protein in C2C12 cells. Conclusions/Significance The present study has identified key inhibitory mechanisms featuring miRNAs that regulate utrophin expression, and demonstrated that these mechanisms can be targeted to increase endogenous utrophin expression in cultured muscle cells. We suggest that miRNA-mediated inhibitory mechanisms could be targeted by methods similar to those described here as a novel strategy to increase utrophin expression as a therapy for DMD

Functional characteristics of calcitonin gene-related peptide receptors in human Ewing's sarcoma WE-68 cells

AbstractCalcitonin gene-related peptide (CGRP) receptor activity was studied in WE-68 human Ewing's sarcoma cells. 125I-human CGRP bound in a time-dependent, reversible and saturable manner. Scatchard plots were compatible with the presence of a homogenous population of CGRP receptors with high affinity (Kd = 15 pM, and Bmax = 1.9 fmolmg protein). The potency order of unlabeled peptides, in the presence of radioligand, was: human CGRP-II > human CGRP = chick CGRP > rat CGRP = rat [Tyro]CGRP > human [Tyro] CGRP > > salmon calcitonin (CT) > rat [Tyro]CGRP-(28-37). Each peptide except CT and [Tyio]CGRP-(28-37) stimulated cyclic AMP generation in a concentration-dependent manner, and the relative potencies paralleled their relative ability in inhibiting 125I-human CGRP binding. We conclude that WE-68 Ewing's sarcoma cells express genuine CGRP receptors which upon activation lead to stimulation of cyclic AMP formation.Calcitonin gene-related peptide; Calcitonin; cyclic AMP; (Human; Ewing's sarcoma cell

Regeneration of Soft Tissues Is Promoted by MMP1 Treatment after Digit Amputation in Mice

The ratio of matrix metalloproteinases (MMPs) to the tissue inhibitors of metalloproteinases (TIMPs) in wounded tissues strictly control the protease activity of MMPs, and therefore regulate the progress of wound closure, tissue regeneration and scar formation. Some amphibians (i.e. axolotl/newt) demonstrate complete regeneration of missing or wounded digits and even limbs; MMPs play a critical role during amphibian regeneration. Conversely, mammalian wound healing re-establishes tissue integrity, but at the expense of scar tissue formation. The differences between amphibian regeneration and mammalian wound healing can be attributed to the greater ratio of MMPs to TIMPs in amphibian tissue. Previous studies have demonstrated the ability of MMP1 to effectively promote skeletal muscle regeneration by favoring extracellular matrix (ECM) remodeling to enhance cell proliferation and migration. In this study, MMP1 was administered to the digits amputated at the mid-second phalanx of adult mice to observe its effect on digit regeneration. Results indicated that the regeneration of soft tissue and the rate of wound closure were significantly improved by MMP1 administration, but the elongation of the skeletal tissue was insignificantly affected. During digit regeneration, more mutipotent progenitor cells, capillary vasculature and neuromuscular-related tissues were observed in MMP1 treated tissues; moreover, there was less fibrotic tissue formed in treated digits. In summary, MMP1 was found to be effective in promoting wound healing in amputated digits of adult mice. © 2013 Mu et al

Muscle inactivation of mTOR causes metabolic and dystrophin defects leading to severe myopathy

mTor, acting mainly via mTORC1, controls dystrophin transcription in a raptor- and rictor-independent mechanism