Social isolation stress and chronic glutathione deficiency have a common effect on the glutamine-to-glutamate ratio and myo-inositol concentration in the mouse frontal cortex.

Environmental stress can interact with genetic predisposition to increase the risk of developing psychopathology. In this work, we tested the hypothesis that social isolation stress interacts with impaired glutathione synthesis and have cumulative effects on the neurochemical profile of the frontal cortex. A mouse model with chronic glutathione deficit induced by knockout (-/-) of the glutamate-cysteine ligase modulatory subunit (Gclm) was exposed to social isolation stress from weaning to post-natal day 65. Using magnetic resonance methods at high-field (14.1 T), we analysed the neurochemical profile in the frontal cortex, brain size and ventricular volume of adult animals. Glutathione deficit was accompanied by elevated concentrations of N-acetylaspartate, alanine, and glutamine, as well as the ratio of glutamine-to-glutamate (Gln/Glu), and by a reduction in levels of myo-inositol and choline-containing compounds in the frontal cortex of -/- animals with respect to wild-type littermates. Although there was no significant interaction between social isolation stress and glutathione deficiency, mice reared in isolation displayed lower myo-inositol concentration (-8.4%, p < 0.05) and larger Gln/Glu (+7.6%, p < 0.05), relative to those in group housing. Furthermore, glutathione deficiency caused a reduction in whole brain volume and enlargement of ventricles, but social isolation had no effect on these parameters. We conclude that social isolation caused neurochemical alterations that may add to those associated to impaired glutathione synthesis

Beside and behind the wheel : factors that influence driving stress and driving behavior

A large percentage of traffic accidents are due to human errors. Driving behavior and driving stress influence the probability of making these mistakes. Both are influenced by multiple factors, among which might be elements such as age, gender, sleeping hours, or working hours. The objective of this paper is to study, in a real scenario and without forcing the driver?s state, the relationship between driving behavior, driving stress, and these elements. Furthermore, we aim to provide guidelines to improve driving assistants. In this study, we used 1050 driving samples obtained from 35 volunteers. The driving samples correspond to regular commutes from home to the workplace. ANOVA and ANCOVA tests were carried out to check if there are significant differences in the four factors analyzed. Although the results show that driving behavior and driving stress are affected by gender, age, and sleeping hours, the most critical variable is working hours. Drivers with long working days suffer significantly more driving stress compared to other drivers, with the corresponding effect on their driving style. These drivers were the worst at maintaining the safety distance.5311-8814-F0ED | Sara Maria da Cruz Maia de Oliveira PaivaN/

Glutathione Deficit Affects the Integrity and Function of the Fimbria/Fornix and Anterior Commissure in Mice: Relevance for Schizophrenia.

Structural anomalies of white matter are found in various brain regions of patients with schizophrenia and bipolar and other psychiatric disorders, but the causes at the cellular and molecular levels remain unclear. Oxidative stress and redox dysregulation have been proposed to play a role in the pathophysiology of several psychiatric conditions, but their anatomical and functional consequences are poorly understood. The aim of this study was to investigate white matter throughout the brain in a preclinical model of redox dysregulation. In a mouse model with impaired glutathione synthesis (Gclm KO), a state-of-the-art multimodal magnetic resonance protocol at high field (14.1 T) was used to assess longitudinally the white matter structure, prefrontal neurochemical profile, and ventricular volume. Electrophysiological recordings in the abnormal white matter tracts identified by diffusion tensor imaging were performed to characterize the functional consequences of fractional anisotropy alterations. Structural alterations observed at peri-pubertal age and adulthood in Gclm KO mice were restricted to the anterior commissure and fornix-fimbria. Reduced fractional anisotropy in the anterior commissure (-7.5% ± 1.9, P<.01) and fornix-fimbria (-4.5% ± 1.3, P<.05) were accompanied by reduced conduction velocity in fast-conducting fibers of the posterior limb of the anterior commissure (-14.3% ± 5.1, P<.05) and slow-conducting fibers of the fornix-fimbria (-8.6% ± 2.6, P<.05). Ventricular enlargement was found at peri-puberty (+25% ± 8 P<.05) but not in adult Gclm KO mice. Glutathione deficit in Gclm KO mice affects ventricular size and the integrity of the fornix-fimbria and anterior commissure. This suggests that redox dysregulation could contribute during neurodevelopment to the impaired white matter and ventricle enlargement observed in schizophrenia and other psychiatric disorders

ARTEMISA: Architecture of an eco-driving assistant based on the anticipation

This paper presents the architecture of an eco driving assistant. The assistant evaluates the fulfil ment of classic eco-driving advices such as: main tain a constant speed, driving at high gear, slow down smoothly and so on. In addition, the assistant issues advices based on the anticipation. Anticipa tion is the key of eco-driving. The assistant is ca pable of detecting traffic signs beforehand and it checks if the speed is suitable for not having to slow down sharply. In addition, the system propos es an optimal average speed according to the con ditions of the road. To model the environment where the vehicle is moving, we use an Android mobile device. These devices are ideal due to to their multiple network connections (Bluetooth, UTMS and WIFI) and sen sors (camera, acceleration sensor, GPS and so on). To obtain the vehicle’s parameters (speed, fuel consumption, RPM, etc.), we use the diagnostic port (OBD2). The proposed system can improve fuel consump tion and safety. In addition, it is independent of the type of vehicle.Ministerio de Ciencia e Innovación TIN2009-14378-C02-02European Union REA FP7/2007-2013 n° 286533Ministerio de Ciencia e Innovación HAUS IPT-2011-1049-43000

Analysis of mobility changes caused by COVID-19 in a context of moderate restrictions using data collected by mobile devices

Since its inception, COVID-19 has changed several dynamics in society, both on a personal and professional level. Mobility was one of the most affected aspects, as a result of the necessary social distancing and preventive measures that had to be enacted by the various countries and which restricted, at various times, freedom of movement. The impact that COVID-19 had, and still has, on mobility is important to be understood so that the necessary measures can be taken in order to return to normality and, for example, not regress in the steps that were being taken in encouraging the use of public transport as a measure to combat the carbon footprint as well as traffic congestion in cities. This paper intends to analyze the reality of Spain and Portugal, in the period between May 10th and July 2nd, 2021, in which both countries had already finished restricting mobility measures. The study used data from Google Community Mobility Reports and was done by regions, taking into account the average age of inhabitants and the number of inhabitants in each region. The analysis focused on different categories of places such as retail and recreation, groceries and pharmacies, parks, transit stations, workplaces, and residential. One of the main conclusions of this study is the lower use of public transports for fear of a greater risk of infection. This could be a problem in the medium term if this trend continues. On the other hand, it is important to highlight a greater presence in parks after the end of the lockdown, which promotes healthy habits that combat problems such as overweight or obesity. COVID-19 can be seen as an opportunity to promote more active mobility through the creation of infrastructure, such as for bicycles.5311-8814-F0ED | Sara Maria da Cruz Maia de Oliveira PaivaN/

COVID-19 and its effects on the driving style of spanish drivers

Spain is one of the countries most affected by COVID-19. Until May 5, 2021 there have been 3.54 million people infected and 78,399 deaths according to the Johns Hopkins Institute. This has meant that Spain is one of the countries in the world where the most restrictive measures have been adopted and which have had a strong impact on the economic, social, and mental health of citizens. One of the consequences of this pandemic is a severe increase in the strain on the healthcare system. Many of those infected had to be admitted to hospitals and even to Intensive Care Units (ICU). In this scenario, it is essential to reduce traffic accidents in order to avoid overcrowded hospitals. However, the Spanish General Directorate of Traffic highlights in several traffic reports that despite the decrease in the volume and distance of trips, the number of traffic accidents has not altered significantly and even during some periods has been higher than before the pandemic. The aim of this work is to establish if there has been a change in the driving style of drivers during the pandemic and to identify the group of people adopting a more inefficient and unsafe driving style. To this end, a study was carried out with 30 volunteers and their current driving style was compared with their driving style before the pandemic. The results show that drivers have adopted a more aggressive driving style than before the pandemic, especially women and those who have suffered anxiety due to COVID-19.5311-8814-F0ED | Sara Maria da Cruz Maia de Oliveira PaivaN/

Single paternal dexamethasone challenge programs offspring metabolism and reveals multiple candidates in RNA-mediated inheritance.

Single traumatic events that elicit an exaggerated stress response can lead to the development of neuropsychiatric conditions. Rodent studies suggested germline RNA as a mediator of effects of chronic environmental exposures to the progeny. The effects of an acute paternal stress exposure on the germline and their potential consequences on offspring remain to be seen. We find that acute administration of an agonist for the stress-sensitive Glucocorticoid receptor, using the common corticosteroid dexamethasone, affects the RNA payload of mature sperm as soon as 3 hr after exposure. It further impacts early embryonic transcriptional trajectories, as determined by single-embryo sequencing, and metabolism in the offspring. We show persistent regulation of tRNA fragments in sperm and descendant 2-cell embryos, suggesting transmission from sperm to embryo. Lastly, we unravel environmentally induced alterations in sperm circRNAs and their targets in the early embryo, highlighting this class as an additional candidate in RNA-mediated inheritance of disease risk.KG was funded by the Swiss National Science Foundation early postdoc and advanced postdoc mobility a SPARK and Novartis foundation grant. Some of this work was supported by Cancer Research UK (C13474/A18583, C6946/A14492) and Wellcome (104640/Z/14/Z, 092096/Z/10/Z) to EAM. GP and MH were supported by a core grant from the Wellcome Trust. The lab of JB is currently funded by the ETH Zurich, SNSF Project Grant 310030_172889/1, ETH Research Grant ETH-20 19-1, the Kurt und Senta Herrmann-Stiftung, the Botnar Research Center for Child Health and a 3R Competence Center Project Grant. JK was supported by a Swiss-european mobility programme scholarship

User preferences in the design of advanced driver assistance systems

The transport network and mobility aspects are constantly changing, and major changes are expected in the coming years in terms of safety and sustainability purposes. In this paper, we present the main conclusions and analysis of data collected from a survey of drivers in Spain and Portugal regarding user preferences, highlighting the main functionalities and behavior that an advanced driver assistance system must have in order to grant it special importance on the road to prevent accidents and also to enable drivers to have a pleasant journey. Based on the results obtained from the survey, we developed and present a working prototype for an advanced driver assistance system (ADAS), its architecture and rules systems that allowed us to create and test some scenarios in a real environment.5311-8814-F0ED | Sara Maria da Cruz Maia de Oliveira PaivaN/

Redox dysregulation, neuroinflammation, and NMDA receptor hypofunction: A "central hub" in schizophrenia pathophysiology?

Accumulating evidence points to altered GABAergic parvalbumin-expressing interneurons and impaired myelin/axonal integrity in schizophrenia. Both findings could be due to abnormal neurodevelopmental trajectories, affecting local neuronal networks and long-range synchrony and leading to cognitive deficits. In this review, we present data from animal models demonstrating that redox dysregulation, neuroinflammation and/or NMDAR hypofunction (as observed in patients) impairs the normal development of both parvalbumin interneurons and oligodendrocytes. These observations suggest that a dysregulation of the redox, neuroimmune, and glutamatergic systems due to genetic and early-life environmental risk factors could contribute to the anomalies of parvalbumin interneurons and white matter in schizophrenia, ultimately impacting cognition, social competence, and affective behavior via abnormal function of micro- and macrocircuits. Moreover, we propose that the redox, neuroimmune, and glutamatergic systems form a "central hub" where an imbalance within any of these "hub" systems leads to similar anomalies of parvalbumin interneurons and oligodendrocytes due to the tight and reciprocal interactions that exist among these systems. A combination of vulnerabilities for a dysregulation within more than one of these systems may be particularly deleterious. For these reasons, molecules, such as N-acetylcysteine, that possess antioxidant and anti-inflammatory properties and can also regulate glutamatergic transmission are promising tools for prevention in ultra-high risk patients or for early intervention therapy during the first stages of the disease