人工气候箱在“六淫”研究中的应用

“六淫“的发生与气候密切相关,人工气候箱可以模拟出自然界可能出现的各种气候类型,为“六淫“实验研究的最重要手段之一,已在风、寒、湿、燥邪等研究中取得一定成果。然而由于气候对人体的影响规律尚不明确,所以研究中还存在同种邪气气候箱条件设置各异、动物在气候箱放置时间各异等问题,也是“六淫“研究今后要深入探讨的问题。认为随着人工气候箱的发展,可以模拟出更精确、复杂的气候环境,可望在“六淫“和其他气候相关科研中发挥更大作用。国家自然科学基金资助项目(81302876); 福建省自然科学基金资助项目(2013J05123

Bioaccumulation of nickel from aqueous solutions by genetically engineered Escherichia coli SE5000

[中文文摘]利用基因工程菌E .coliSE5 0 0 0对水体中的镍离子进行富集研究 .菌体细胞对Ni2 + 的富集速率很快 ,富集过程满足Langmuir等温线模型 .经基因改造的基因工程菌不仅最大镍富集容量与原始宿主菌相比增加了 4倍多 ,而且对pH值的变化呈现出更强的适应性 .对离子强度及其它共存重金属离子的影响的实验结果表明 :Na+ ,Ca2 + ,Cd2 + ,Pb2 + 的影响较小 ,但Mg2 + ,Hg2 + ,Cr3 + 和Cu2 + 所引起的负面效应较大 .金属螯合剂EDTA的存在对基因工程菌的富集行为影响很大.[英文文摘]A genetically engineered E. coli SE5000 strain which simultaneously expressed nickel transport system and metallothionine was used to accumulate Ni2 + from aqueous solution. Bioaccumulation process was rapid and followed linearized Langmuir isotherm. A more than fourfolds increase of Ni2 + binding capacity was obtained by genetically engineered E. coli cells compared with original host E. coli cells. A pH assay showed that genetically engineered E. coli cells could accumulate Ni2 + effectively over a broad range of pH (4 to10) and the optimal pH was 816. The presence of 1000mg·L- 1 Na + and Ca2 + , or 50mg·L - 1 Cd2 + or Pb2 + did not have obvious effects on Ni2 + bioaccumulation , while Mg2 + , Hg2 + , Cr3 + and Cu2 + posed severe adverse effects on Ni2 + uptake by genetically engineered E. coli . The presence of EDTA could inhibit nickel uptake.2003年教育部优秀青年教师资助计划; 浙江省自然科学基金 (M203130 )

第三代移动通信系统的发展分析

文章回顾了移动通信技术从第一代到第二代的演进,对发展第三代移动通信所面临的问题与提交给ITU各种技术标准进行了简单说明,进而重点介绍第三代移动通信标准(ITU-RSPC)、系统结构及基本特征

Effects of night-time warming on the rates and main chemical components of root exudates produced by Picea asperata seedlings in subalpine coniferous forests

以连续7a红外辐射增温处理下的云杉幼苗为对象,定量分析了增温对植物根系碳(C) 、氮(N)分泌速率以及主要挥发性化学成分的影响。结果表明: (1)增温显著增加了云杉幼苗根系C分泌速率,而对N分泌速率无显著影响,并伴随着根系分泌物C ∶N化学计量比显著增加; (2)不同化学组分输入含量变化对增温的响应具有明显差异,其响应幅度和方向与化学组分种类有关。其中糖类、氨基酸和酚类化合物的含量在增温处理下均显著增加,而酯类、醚类相对含量显著降低; (3)进一步分析表明,同组分中不同化合物成分含量对增温的响应也有所差异。例如,增温仅导致酚类化合物中2,6-二叔丁基-4-甲基苯酚和4-叔丁基杯[4]芳烃含量显著增加(分别比对照增加了88.9%和375.7%),而对其余酚类化合物成分含量无显著影响。结果表明增温可导致植物根系分泌物各组分相对含量发生深刻地变化,这对于进一步认识不同环境变化下根系分泌物输入及其所诱导的特异性土壤微生物养分过程具有重要的理论意义

氮素富集对青藏高原东缘窄叶鲜卑花灌丛根系分泌物碳输入的影响

为探究高寒灌丛生态系统根系分泌物碳(C)输入通量对大气氮(N)沉降的响应规律,该文以青藏高原东缘窄叶鲜卑花(Sibiraea angustata)灌丛为研究对象,采用根系分泌物野外原位收集法,分析了不同施N水平(对照N0=0 g·m~(–2)·a~(–1);低N处理N5=5 g·m~(–2)·a~(–1);高N处理N10=10 g·m~(–2)·a~(–1))对根系分泌物C输入速率与通量季节动态变化规律的影响。结果表明:(1)窄叶鲜卑花灌丛单位根生物量、单位根长、单位根表面积根系分泌物C输入速率均表现出明显的季节性动态变化,具体表现为8月>6月>10月,并呈现出与5 cm土壤温度相一致的变化趋势。(2)施N降低了窄叶鲜卑花灌丛单位根生物量、单位根长和单位根表面积根系分泌物C输入速率,但仅N10处理与对照(N0处理)间存在显著差异(p<0.05)。(3)N5和N10处理下,窄叶鲜卑花灌丛细根生物量与N0处理相比分别降低了23.36%和33.84%。(4)由于施N导致根系分泌物C输入速率与细根生物量二者均显著降低,使得施N对窄叶鲜卑花灌丛根系分泌物C输入通量(g·m~(–2)·a~(–1))有显著的抑制作用,并随着施N浓度的增加抑制作用增大。推测其可能的原因是N素富集在一定程度上缓和了植物根系对养分的微生物驱动需求,从而降低了植物根系分泌物C输入通量,即N素富集条件下植物采取了低N收益-低C投入的生理策略。该研究结果对于进一步认知不同环境变化下高寒灌丛生态系统根系分泌物C输入及其介导的土壤生物C-养分循环过程具有重要的理论意义

Effect of Sanren Tang (三仁汤) on Mucin 5AC, Aquaporin 1 and Aquaporin 5 in Lung Tissue of Damp-heat Stressed Mice

目的探讨三仁汤防治湿热环境下呼吸系统疾病的可能作用机制。方法72只小鼠随机分为空白组、模型组、三仁汤组,每组24只。空白组普通环境喂养,其余两组; 置入气候箱接受高温高湿刺激。三仁汤组小鼠于放入气候箱前、放入气候箱后第12h分别予三仁汤(浓度1g/ml)0.2ml灌胃各1次,空白组和模型组给; 予等量生理盐水灌胃。放入气候箱后第12h、24h、4天时每组取材8只,检测肺组织黏蛋白5AC; (MUC5AC)、水通道蛋白1(AQP1)、水通道蛋白5(AQP5)表达水平。结果模型组第24h时MUC5AC水平高于空白组,三仁汤组第12h; 、24h时MUC5AC表达低于模型组(P0.05)。各组不同时间点AQP1表达水平比较差异均无统计学意义(P>0.; 05)。结论三仁汤可能通过促进肺组织AQP5表达,从而抑制MUC5AC分泌,阻止湿热环境下呼吸系统疾病气道黏液高分泌状态进展。Objective To explore the possible mechanism of Sanren Tang (三仁汤) in; preventing and treating respiratory diseases under damp-heat; circumstances. Methods Seventy-two mice were randomized into blank; group, model group and Sanren Tang group, with 24 mice in each group.; The blank group was fed under conventional environment, while the other; two groups were put into climate chamber with high temperature and high; humidity. The Sanren Tang group was given Sanren Tang (1g/ml 0.2ml)by; gavage before and at the 12th hour of being put into climate chamber.; The blank group and model group were given equivalent normal saline by; gavage. At the 12th, 24th hour and on the 4th day after being put into; climate chamber, eight mice in each group were selected to draw; materials, and the levels of mucin 5AC (MUC5AC), aquaporin 1 (AQP1) and; aquaporin 5 (AQP5) were detected. Results The 24th-hour MUC5AC level in; the model group was higher than that in the blank group; the MUC5AC; level at the 12th and the 24th hour in Sanren Tang group was; respectively lower than that in the model group (P0.05). In each group, there was no significant difference of AQP1; level at different time points (P>0.05). Conclusion By promoting the; expression of AQP5 in lung tissue, Sanren Tang may inhibit the secretion; of MUC5AC, and prevent the airway mucus hypersecretion in respiratory; diseases under damp-heat circumstances.国家自然科学基金项目; 福建省自然科学基

2000–2010年中国典型陆地生态系统实际蒸散量和水分利用效率数据集

蒸散是陆地生态系统水分循环和能量平衡的关键过程,水分利用效率是反映生态系统碳水循环间耦合关系的重要指标,二者在生态学、农学、水文学、气候学等多个学科中均具有重要的应用价值。涡度相关法被认为是现今唯一能直接测量生物圈与大气间物质与能量交换通量的标准方法,已成为生态系统尺度碳水交换通量观测的主要方法。本文通过整合中国陆地生态系统通量观测联盟(China FLUX)的长期观测数据和中国区域其他观测站点基于涡度相关法发表的文献数据,构建了一套中国典型陆地生态系统实际蒸散量和水分利用效率数据集。本数据集共有实际蒸散量数据记录143条、水分利用效率数据记录96条,涉及5种生态系统类型45个生态系统,时间跨度为2000–2010年。本数据集可以为陆地生态系统碳水循环、生态系统管理和评估、全球变化等相关领域的研究提供数据支持

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel