Genotype-renal function correlation in type 2 autosomal dominant polycystic kidney disease

Autosomal dominant polycystic kidney disease (ADPKD) is a common Mendelian disorder that affects approximately 1 in 1000 live births. Mutations of two genes, PKD1 and PKD2, account for the disease in approximately 80 to 85% and 10 to 15% of the cases, respectively. Significant interfamilial and intrafamilial renal disease variability in ADPKD has been well documented. Locus heterogeneity is a major determinant for interfamilial disease variability (i.e., patients from PKD1-linked families have a significantly earlier onset of ESRD compared with patients from PKD2-linked families). More recently, two studies have suggested that allelic heterogeneity might influence renal disease severity. The current study examined the genotype-renal function correlation in 461 affected individuals from 71 ADPKD families with known PKD2 mutations. Fifty different mutations were identified in these families, spanning between exon 1 and 14 of PKD2. Most (94%) of these mutations were predicted to be inactivating. The renal outcomes of these patients, including the age of onset of end-stage renal disease (ESRD) and chronic renal failure (CRF; defined as creatinine clearance less than or equal to 50 ml/min, calculated using the Cockroft and Gault formula), were analyzed. Of all the affected individuals clinically assessed, 117 (25.4%) had ESRD, 47 (10.2%) died without ESRD, 65 (14.0%) had CRF, and 232 (50.3%) had neither CRF nor ESRD at the last follow-up. Female patients, compared with male patients, had a later mean age of onset of ESRD (76.0 [95% CI, 73.8 to 78.1] versus 68.1 [95% Cl, 66.0 to 70.2] yr) and CRF (72.5 [95% Cl, 70.1 to 74.9] versus 63.7 [95% CI, 61.4 to 66.0] yr). Linear regression and renal survival analyses revealed that the location of PKD2 mutations did not influence the age of onset of ESRD. However, patients with splice site mutations appeared to have milder renal disease compared with patients with other mutation types (P < 0.04 by log rank test; adjusted for the gender effect). Considerable renal disease variability was also found among affected individuals with the same PKD2 mutations. This variability can confound the determination of allelic effects and supports the notion that additional genetic and/or environmental factors may modulate the renal disease severity in ADPKD

Study On Optimization Of Group Control System Of A Group LTD.

A集团是厦门市属的国有企业集团之一，自成立以来，为主承担厦门大中型基础设施建设工程及城市片区开发的投资建设任务。经过历届领导班子和全体员工多年来的辛勤付出和艰苦创造，A集团自成立至今在交通基础设施投资建设领域取得了辉煌的成就，并在此基础上延伸出了相关与非相关产业的数家企业。但是A集团目前存在着集团规划目标与组织行为存在偏差、集团总部职能定位不清晰、对权属企业的管控模式模糊、集团内控体系不完善等严峻问题，这些问题若不得到有效的解决，将严重影响A集团的整体发展。 企业集团管控课题纷繁复杂、体系庞大。本文从A集团现状和问题入手，采取理论与实践相结合的办法。在分析国内外集团管控体系理论研究成果的基础...How to build an effective group control system to guarantee uniformity and maximized benefits between parent and subsidiary company, is an important topic and a long-term focus of management theories, and also a management weapon in urgent need by many enterprise group to cope with the sharp market competition. In 23 years’ accumulation and development, A group has 7 wholly-owned and holding s...学位：工商管理硕士院系专业：管理学院_工商管理硕士(工商管理硕士)学号：1792011115070

Medium optimization for Schizochytrium sp. to produce docosahexaenoic acid (DHA)

以筛选得到的Schizochytrium sp.菌株作为研究对象,考察了培养基碳源、氮源对该菌株生物量的影响,通过响应面法建立了菌体生物量与葡萄糖、酵母粉以及玉米浆浓度之间的关系,得到较佳培养基组成为:126g/L葡萄糖、10g/L酵母膏、2g/L玉米浆、5g/L蛋白胨、0.5倍浓度自然海水1L。摇瓶发酵结果表明:在5d培养时间,Schizochytriumsp.生物量、积累油脂以及DHA分别可达42.9、34.1、13.8g/L。One strain of Schizochytrium sp.was selected as the mode microorganism to evaluate the influences of carbon and nitrogen resource on the biomass.By employing response surface method,the relationship between biomass and nutrients was founded and the optimized culture medium for Schizochytrium sp.was determined.The optimized medium consisted of 126 g/L glucose,10 g/L yeast extract,2 g/L corn steep liquor,5 g/L soy peptone and half the salt concentration of seawater 1 L.The maximum biomass,total fatty acid and DHA reached 42.9 g/L,34.1 g/L and 13.8 g/L on the optimized culture medium after 5 days incubation,respectively

关于尽快启动我国“灾害金融”问题研究的思考

我国是世界上受自然灾害影响最为严重的国家之一,目前所使用的防灾减灾金融手段大多具有明显的行政应急色彩,且后续负效应较大,难以作为一种应对自然灾害的长期措施而加以广泛应用。笔者认为,我国应尽快启动"灾害金融"问题研究,以填补我国在该领域的研究空白;同时,通过对灾害金融工具、灾害金融运行机制、灾害金融效应评估等问题的研究,构建中国灾害金融的理论研究与运行体系,为我国灾后急救与重建提供高效、有序的金融支持。教育部人文社会科学一般项目成果,项目批准号:07JA79003

束缚和异氟烷麻醉对大鼠心率变异性及HPA轴影响的比较

目的为了观察束缚固定和异氟烷(流量:0.8 L,浓度:1.5%)麻醉状态中大鼠的心率变异性的变化;并通过比较9天每天30 min束缚干预和异氟烷麻醉对大鼠的体重、痛阈以及与HPA轴相关激素的影响,来评估长期使用异氟烷麻醉和束缚固定对大鼠应激程度的影响,选择更适宜的大鼠固定方法,为基础医学的实验方法提供重要的参考。方法 SD大鼠随机分为3组:空白组,束缚组,异氟烷麻醉组。急性实验通过记录大鼠心电图15 min,观察束缚固定和异氟烷麻醉情况下大鼠心率,心率变异性的变化。慢性实验中比较连续干预9天(30 min/d)前后3组大鼠体重、痛阈变化以及与应激状态相关激素含量的变化。结果 1)急性试验:与空白组大鼠相比,束缚干预和异氟烷麻醉均造成大鼠心率显著增加,具有统计学意义(P 0.05)。结论以上结果提示:低浓度的异氟烷造成麻醉中大鼠心率增加,心率变异性下降,产生以交感兴奋为主的自主神经功能状态改变。多次束缚固定易造成大鼠HPA轴激活,产生慢性应激的效应。在长期需要固定动物的实验中,与束缚固定相比异氟烷麻醉是更好的固定方法。国家自然科学基金(81674083

Genetic engineering of E-coli SE5000 and its potential for Ni2+ bioremediation

A genetically engineered Escherichia coli SE5000 strain simultaneously expressing nickel transport system and metallothionein was constructed to accumulate Ni2+ from aqueous solution. Bioaccumulation was fast and followed linearized Langmuir isotherm. Compared with 1.62 mg/g of Ni2+ uptake capacity by original host E. coli cells, genetically engineered E. coli could bind 7.14 mg/g Ni2+, and it accumulated Ni2+ effectively over a broad range of pH (4-10) and the optimal pH was 8.6. The presence of 1000mg/l Na+ and Ca2+ or 50 mg/l Cd2+ and Pb2+ did not decrease Ni2+ bioaccumulation significantly, but Mg2+, Hg2+, Cr3+ and Cu2+ posed severe deleterious influences on Ni2+ uptake by genetically engineered E. coli. Furthermore, the presence of EDTA inhibited nickel bioaccumulation. (C) 2004 Elsevier Ltd. All rights reserved

戊型肝炎病毒抗体双抗原夹心法ELISA的建立与初步应用

目的　建立抗戊型肝炎病毒 (HEV)抗体检测的双抗原夹心ELISA(DS -ELISA) ,并应用于多种动物血清的检测。方法　将大肠杆菌表达的一段HEVORF2区重组抗原分别包被微孔板和进行辣根过氧化物酶 (HRP)标记 ,利用 5份阳性血清和 4 0份阴性血清建立双抗原夹心ELISA ;用 4 0 0份义务献血员血清比较双抗原夹心ELISA与间接法IgG抗体ELISA的符合情况 ;用 3只HEV感染猴系列血清比较双抗原夹心ELISA试剂和Genelabs公司HEVIgG试剂 ;用双抗原夹心ELISA试剂检测新疆地区的部分牛、绵羊、山羊、猪血清和上海地区的部分鸡血清中的HEV抗体。结果　建立了检测HEV抗体的双抗原夹心ELISA方法 ,对 4 0 0份义务献血员血清的检测表明其与间接法IgG抗体ELISA试剂的符合情况良好 ,并且有更高的s/co比值 ;与Genelabs公司HEVIgG试剂的比较表明双抗原夹心ELISA试剂的检出更早 ,尤其是持续时间及强度明显优于Genelabs试剂 ;在所检测的各种动物中均发现了HEV抗体 ,其中猪抗体的阳性率最高 ,表明双抗原夹心ELISA试剂可同时用于不同动物的抗HEV抗体检测。结论　利用大肠杆菌表达的HEV重组抗原建立了双抗原夹心法ELISA ,并可同时用于不同动物的抗HEV抗体检测

Production of β-glucanase by cultivation of immobilized recombinant E.coli cells in a packed bed reactor

以多孔陶瓷为载体吸附法固定重组大肠杆菌E.coli JM 109-pLF3表达胞外β-葡聚糖酶。考察了固定床间歇培养时循环流速和曝气量对发酵液酶活力的影响。当循环流速达到44.19mL/min,曝气量达到0.6mL/min时,培养48h后,发酵液的酶活力达100.3U/mL。固定化细胞具有良好的重复使用能力,在连续5批次实验中,培养48h后的酶活力均在100U/mL左右。固定床连续培养时,固定化细胞能够保持恒定的产酶效率,当稀释率为0.05h-1时,发酵液中得到的酶活力为39.1U/mL。β-glucanase produced by immobilized Escherichia coli JM 109-pLF3 in a packed bed bioreactor was studied.In batch fermentation,the highest enzyme activity of 100.3 U/mL was achieved at a recycling rate of 44.49 mL/min and an aeration rate of 0.6 mL/min after 48 h cultivation.In repeated batch operation,the enzyme activity up to 100 U/mL was obtained in 5 cycless,howing high stability of the immobilized cells.In continuous fermentation,the production of β-glucanase by immobilized cells was kept more or less constant.When the dilution rate was 0.05 h-1,the enzyme activity in the fermentation broth was 39.1 U/mL.厦门市科技计划项目(No.3502Z20055017

生物法制备纳米银溶胶的稳定性

利用生物还原法制备纳米银溶胶,借助于UV-Vis表征技术对其热稳定性和化学稳定性进行考察。结果表明:生物法制备的纳米银溶胶在100℃下加热6h,UV-Vis谱图未发生明显变化;H+和具有高价阳离子的电解质对其稳定性的影响明显;OH-对银溶胶的稳定性影响相对较弱。生物法制备的纳米银溶胶在热稳定性、化学稳定性方面均略优于柠檬酸三钠法制得的银溶胶

Component analysis and risk assessment of anaerobically digested slurry from households in China

采集了中国8个省市的43个不同原料家庭户用沼气池的沼液,进行化学需氧量(COd)、氨氮(nH+4-n)、磷酸盐(PO43？)和重金属等指标分析,阐述不同原料导致沼液成分的差异,评价了不同来源沼液存在的生态风险,旨在为不同来源沼液的资源化利用提供理论依据。研究结果表明,以牛粪和秸秆为原料的沼液COd浓度较高,分别达到6800 Mg·l？1和5800 Mg·l？1;以猪粪和混合粪便为原料的沼液氨氮浓度较高,都超过1800 Mg·l？1,而牛粪原料沼液氨氮浓度明显低于其他原料,平均值仅有450 Mg·l？1,因此以牛粪为原料的沼液COd/nH+4-n显著高于其他三种原料,COd/nH+4-n达到15,而其他三种原料的沼液COd/nH+4-n均低于5;所有原料沼液磷酸盐浓度的平均值均低于80 Mg·l？1;沼液中汞的污染较严重,且在不同原料和不同地区的沼液中具有普遍性;潜在生态风险指数rI分析结果表明,云南、河南和湖北的沼液rI介于130~260之间,属于中等生态危害,存在一定的生态风险。Forty-three biogas slurries from household biogas plants using different substrates were collected from different parts of China.The contents of chemical oxygen demand(COD), ammonium nitrogen(NH+ 4-N), phosphate(PO4 3?) and heavy metals were determined.The concentrations of COD in cow dung and straw as raw materials were higher than other treatments, reaching 6800 mg·L?1 and 5800 mg·L?1 respectively.The concentrations of NH+ 4-N in pig manure and mix manure were higher than other treatments, reaching more than 1800 mg·L?1..The concentration of NH+ 4-N in cow dung was significantly lower than that in other materials, with the average being 450 mg·L?1.Therefore, COD/ NH+ 4 ratio of 15 in the slurry with cow dung as raw material was significantly higher than those in other three materials(less than 5).The average PO4 3? concentrations of all raw biogas were lower than 80 mg·L?1.Mercury pollution was serious and universal in different materials and different provinces.Environment risk evaluation showed that the pollution in biogas slurries from Yunnan, Henan and Hubei was medium and could not be ignored.国家重点基础研究发展计划项目(2013CB733505)~