Yield and Woody Biomass Traits of Novel Shrub Willow Hybrids at Two Contrasting Sites

Shrub willow has great potential as a dedicated bioenergy crop, but commercialization and adoption by growers and end-users will depend upon the identification and selection of high-yielding cultivars with biomass chemistry and quality amenable to conversion to biofuels and bioenergy. In this study, critical traits for biomass production were evaluated among new genotypes of shrub willow produced through hybrid breeding. This study assessed the variation in yield, pest and disease resistance, biomass composition, and wood density in shrub willow, as well as the impact of genotypic and environmental factors on these particular phenotypes. Analysis of clonal genotypes established on two contrasting sites in New York State, Tully and Belleville, showed statistical differences by site for all of the traits. The greatest yield was observed at Belleville, NY, for two cultivars, ‘Fish Creek' (41Mg ha−1) and ‘Onondaga' (40Mg ha−1). Yields of Salix eriocephala genotypes were lowest, and they displayed susceptibility to rust and beetle damage. Variation in cellulose content in the stem biomass was controlled by environmental factors, with the majority of the genotypes displaying greater cellulose content at Belleville compared with Tully. In contrast, wood density was significantly greater at Tully than Belleville, and cellulose content was correlated with wood density. There were no significant correlations between biomass yield and density or any of the composition traits. These trials demonstrate that new genotypes produce improved yield and pest and disease resistance, with diverse compositional traits that can be matched with conversion technologie

Correlations of expression of cell wall biosynthesis genes with variation in biomass composition in shrub willow ( Salix spp.) biomass crops

We have measured significant genetically determined variation in biomass composition among breeding populations of shrub willow, a biomass feedstock crop. This project was aimed to ask whether patterns of cell wall gene expression can be correlated with genetic variation in biomass composition at harvest, in order to develop assays of early differences in gene expression as indicators of harvestable biomass chemical composition and potentially reduce the time of selection for new willow genotypes. Previous studies have demonstrated that manipulation of expression of cell wall biosynthetic genes results in altered biomass chemical composition. We analyzed genes encoding enzymes involved in lignin biosynthesis and carbohydrate active enzymes selected based on their functional characterization and conservation in Populus trichocarpa and Arabidopsis thaliana. Fragments of 20 genes were cloned from young stem cDNA of Salix sachalinensis and Salix miyabeana. Expression profiling in willow stem apical tissue and developing stem tissue was performed for each isolated gene using probe-based quantitative real-time PCR. Two willow parental genotypes and six progeny within a hybrid family were selected for analysis, and significant differences in expression among the individuals and between tissue types were observed for most of the genes. Significant correlations between patterns of gene expression and variation in the biomass chemical composition of those genotypes provide insight into the genetic regulation of lignocellulosic deposition in this important bioenergy crop and could be utilized as a tool for early selection of new genotype

Willow Cultivars

The State University of New York College of Environmental Science and Forestry (SUNY-ESF) presents fact sheets on Salix purpurea ‘Allegany’, Salix sachalinensis × S. miyabeana ‘Canastota’, Salix purpurea ‘Fish Creek’, Salix purpurea × S. miyabeana ‘Millbrook’, Salix purpurea × S. miyabeana ‘Oneida’, Salix purpurea ‘Onondaga’, Salix viminalis × S. miyabeana ‘Otisco’, Salix viminalis × S. miyabeana ‘Owasco’, Salix eriocephala ‘S25’, Salix caprea hybrid ‘S365’, Salix sachalinensis × S. miyabeana ‘Sherburne’, Salix × dasyclados ‘SV1’, Salix sachalinensis ‘SX61’, Salix miyabeana ‘SX64’, Salix miyabeana ‘SX67’, Salix viminalis × S. miyabeana ‘Tully Champion’

Early selection of novel triploid hybrids of shrub willow with improved biomass yield relative to diploids

BACKGROUND: Genetic improvement of shrub willow (Salix), a perennial energy crop common to temperate climates, has led to the development of new cultivars with improved biomass yield, pest and disease resistance, and biomass composition suitable for bioenergy applications. These improvements have largely been associated with species hybridization, yet little is known about the genetic mechanisms responsible for improved yield and performance of certain willow species hybrids. RESULTS: The top performing genotypes in this study, representing advanced pedigrees compared with those in previous studies, were mostly triploid in nature and outperformed current commercial cultivars. Of the genotypes studied, the diploids had the lowest mean yield of 8.29 oven dry Mg ha(−1) yr(−1), while triploids yielded 12.65 Mg ha(−1) yr(−1), with the top five producing over 16 Mg ha(−1) yr(−1). Triploids had high stem area and height across all three years of growth in addition to greatest specific gravity. The lowest specific gravity was observed among the tetraploid genotypes. Height was the early trait most correlated with and the best predictor of third-year yield. CONCLUSIONS: These results establish a paradigm for future breeding and improvement of Salix bioenergy crops based on the development of triploid species hybrids. Stem height and total stem area are effective traits for early prediction of relative yield performance

Characterization of a large sex determination region in Salix purpurea L. (Salicaceae).

Dioecy has evolved numerous times in plants, but heteromorphic sex chromosomes are apparently rare. Sex determination has been studied in multiple Salix and Populus (Salicaceae) species, and P. trichocarpa has an XY sex determination system on chromosome 19, while S. suchowensis and S. viminalis have a ZW system on chromosome 15. Here we use whole genome sequencing coupled with quantitative trait locus mapping and a genome-wide association study to characterize the genomic composition of the non-recombining portion of the sex determination region. We demonstrate that Salix purpurea also has a ZW system on chromosome 15. The sex determination region has reduced recombination, high structural polymorphism, an abundance of transposable elements, and contains genes that are involved in sex expression in other plants. We also show that chromosome 19 contains sex-associated markers in this S. purpurea assembly, along with other autosomes. This raises the intriguing possibility of a translocation of the sex determination region within the Salicaceae lineage, suggesting a common evolutionary origin of the Populus and Salix sex determination loci

Captive reptile mortality rates in the home and implications for the wildlife trade

The trade in wildlife and keeping of exotic pets is subject to varying levels of national and international regulation and is a topic often attracting controversy. Reptiles are popular exotic pets and comprise a substantial component of the live animal trade. High mortality of traded animals raises welfare concerns, and also has implications for conservation if collection from the wild is required to meet demand. Mortality of reptiles can occur at any stage of the trade chain from collector to consumer. However, there is limited information on mortality rates of reptiles across trade chains, particularly amongst final consumers in the home. We investigated mortality rates of reptiles amongst consumers using a specialised technique for asking sensitive questions, additive Randomised Response Technique (aRRT), as well as direct questioning (DQ). Overall, 3.6% of snakes, chelonians and lizards died within one year of acquisition. Boas and pythons had the lowest reported mortality rates of 1.9% and chameleons had the highest at 28.2%. More than 97% of snakes, 87% of lizards and 69% of chelonians acquired by respondents over five years were reported to be captive bred and results suggest that mortality rates may be lowest for captive bred individuals. Estimates of mortality from aRRT and DQ did not differ significantly which is in line with our findings that respondents did not find questions about reptile mortality to be sensitive. This research suggests that captive reptile mortality in the home is rather low, and identifies those taxa where further effort could be made to reduce mortality rate

The discovery of a T6.5 subdwarf

We report the discovery of ULAS J131610.28+075553.0, an sdT6.5 dwarf in the UKIDSS Large Area Survey 2 epoch proper motion catalogue. This object displays significant spectral peculiarity, with the largest yet seen deviations from T6 and T7 templates in the Y and K bands for this subtype. Its large, similar to 1 arcsec yr(-1), proper motion suggests a large tangential velocity of V-tan approximate to 240-340 km s(-1), if we assume its M-J lies within the typical range for T6.5 dwarfs. This makes it a candidate for membership of the Galactic halo population. However, other metal-poor T dwarfs exhibit significant under luminosity both in specific bands and bolometrically. As a result, it is likely that its velocity is somewhat smaller, and we conclude it is a likely thick disc or halo member. This object represents the only T dwarf earlier than T8 to be classified as a subdwarf, and is a significant addition to the currently small number of known unambiguously substellar subdwarfs.Peer reviewe

A new benchmark T8-9 brown dwarf and a couple of new mid-T dwarfs from the UKIDSS DR5+ LAS

Benchmark brown dwarfs are those objects for which fiducial constraints are available, including effective temperature, parallax, age, metallicity. We searched for new cool brown dwarfs in 186 sq.deg. of the new area covered by the data release DR5+ of the UKIDSS Large Area Survey. Follow-up optical and near-infrared broad-band photometry, and methane imaging of four promising candidates, revealed three objects with distinct methane absorption, typical of mid- to late-T dwarfs, and one possibly T4 dwarf. The latest-type object, classified as T8-9, shares its large proper motion with Ross 458 (BD+13o2618), an active M0.5 binary which is 102" away, forming a hierarchical low-mass star+brown dwarf system. Ross 458C has an absolute J-band magnitude of 16.4, and seems overluminous, particularly in the K band, compared to similar field brown dwarfs. We estimate the age of the system to be less than 1 Gyr, and its mass to be as low as 14 Jupiter masses for the age of 1 Gyr. At 11.4 pc, this new late T benchmark dwarf is a promising target to constrain the evolutionary and atmospheric models of very low-mass brown dwarfs. We present proper motion measurements for our targets and for 13 known brown dwarfs. Two brown dwarfs have velocities typical of the thick disk and may be old brown dwarfs.Comment: 15 pages, 10 figures and 6 tables. Accepted by MNRAS. Uses mn2e.cls and aas_macr

Ultracool dwarf benchmarks with \emph{Gaia} primaries

This article has been accepted for publication in Monthly Notices of the Royal Astronomical Society. © 2017 The Author(s). Published by Oxford University Press on behalf of the Royal Astronomical Society. All rights reserved.We explore the potential of \emph{Gaia} for the field of benchmark ultracool/brown dwarf companions, and present the results of an initial search for metal-rich/metal-poor systems. A simulated population of resolved ultracool dwarf companions to \emph{Gaia} primary stars is generated and assessed. Of order $\sim$24,000 companions should be identifiable outside of the Galactic plane ($|b| > 10\,$deg) with large-scale ground- and space-based surveys including late M, L, T, and Y types. Our simulated companion parameter space covers $0.02 \le M/M_{\odot} \le 0.1$, $0.1 \le {\rm age/Gyr} \le 14$, and $-2.5 \le {\rm [Fe/H]} \le 0.5$, with systems required to have a false alarm probability $$0.6\, kau}\,Peer reviewedFinal Accepted Versio