Assembly of the SIR Complex and Its Regulation by O-Acetyl-ADP-Ribose, a Product of NAD-Dependent Histone Deacetylation

SummaryAssembly of silent chromatin domains in budding yeast involves the deacetylation of histone tails by Sir2 and the association of the Sir3 and Sir4 proteins with hypoacetylated histone tails. Sir2 couples deacetylation to NAD hydrolysis and the synthesis of a metabolite, O-acetyl-ADP-ribose (AAR), but the functional significance of NAD hydrolysis or AAR, if any, is unknown. Here we examine the association of the Sir2, Sir3, and Sir4 proteins with each other and histone tails. Our analysis reveals that deacetylation of histone H4-lysine 16 (K16), which is critical for silencing in vivo, is also critical for the binding of Sir3 and Sir4 to histone H4 peptides in vitro. Moreover, AAR itself promotes the association of multiple copies of Sir3 with Sir2/Sir4 and induces a dramatic structural rearrangement in the SIR complex. These results suggest that Sir2 activity modulates the assembly of the SIR complex through both histone deacetylation and AAR synthesis

Smoking Profile of Woodside Juvenile Detention Center and Interventional Goal-Setting Workshop

Abstract: We studied smoking status among teenage residents at the Woodside Juvenile Rehabilitation Center (Woodside) in Vermont. Using a modified CDC survey and focus groups, we found that short-term detention residents (S group) were significantly more likely to be “smokers” than the long-term treatment residents (L group). All residents reported that stress had an important impact on their lives. Based on our findings, we implemented a goal setting workshop for both L and S groups.https://scholarworks.uvm.edu/comphp_gallery/1002/thumbnail.jp

Transformations between 2MASS, SDSS and BVI photometric systems for late-type giants

We present colour transformations from Two Micron All Sky Survey (2MASS) photometric system to Johnson-Cousins system and to Sloan Digital Sky Survey (SDSS) system for late-type giants and vice versa. The giant star sample was formed using surface gravity constraints ($2 < \log g \leq 3$) to Cayrel de Strobel et al.\rq s (2001) spectroscopic catalogue. 2MASS, SDSS and Johnson-Cousins photometric data was taken from Cutri et al. (2003), Ofek (2008) and van Leeuwen (2007), respectively. The final sample was refined applying the following steps: (1) the data were dereddened, (2) the sample stars selected are of the highest photometric quality. We give two--colour dependent transformations as a function of metallicity as well as independent of metallicity. The transformations provide absolute magnitudes and distance determinations which can be used in space density evaluations at relatively short distances where some or all of the SDSS magnitudes of late-type giants are saturated.Comment: The variables and "f" parameters in the equations 15-17 and 27-29 were change

Three-Point Correlation Functions of SDSS Galaxies: Luminosity and Color Dependence in Redshift and Projected Space

The three-point correlation function (3PCF) provides an important view into the clustering of galaxies that is not available to its lower order cousin, the two-point correlation function (2PCF). Higher order statistics, such as the 3PCF, are necessary to probe the non-Gaussian structure and shape information expected in these distributions. We measure the clustering of spectroscopic galaxies in the Main Galaxy Sample of the Sloan Digital Sky Survey (SDSS), focusing on the shape or configuration dependence of the reduced 3PCF in both redshift and projected space. This work constitutes the largest number of galaxies ever used to investigate the reduced 3PCF, using over 220,000 galaxies in three volume-limited samples. We find significant configuration dependence of the reduced 3PCF at 3-27 Mpc/h, in agreement with LCDM predictions and in disagreement with the hierarchical ansatz. Below 6 Mpc/h, the redshift space reduced 3PCF shows a smaller amplitude and weak configuration dependence in comparison with projected measurements suggesting that redshift distortions, and not galaxy bias, can make the reduced 3PCF appear consistent with the hierarchical ansatz. The reduced 3PCF shows a weaker dependence on luminosity than the 2PCF, with no significant dependence on scales above 9 Mpc/h. On scales less than 9 Mpc/h, the reduced 3PCF appears more affected by galaxy color than luminosty. We demonstrate the extreme sensitivity of the 3PCF to systematic effects such as sky completeness and binning scheme, along with the difficulty of resolving the errors. Some comparable analyses make assumptions that do not consistently account for these effects.Comment: 27 pages, 21 figures. Updated to match accepted version. Published in Ap

Neutral hydrogen surveys for high redshift galaxy clusters and proto-clusters

We discuss the possibility of performing blind surveys to detect large-scale features of the universe using 21cm emission. Using instruments with approx. 5'-10' resolution currently in the planning stage, it should be possible to detect virialized galaxy clusters at intermediate redshifts using the combined emission from their constituent galaxies, as well as less overdense structures, such as proto-clusters and the `cosmic web', at higher redshifts. Using semi-analytic methods we compute the number of virialized objects and those at turnaround which might be detected by such surveys. We find a surprisingly large number of objects might be detected even using small (approx. 5%) bandwidths and elaborate on some issues pertinent to optimising the design of the instrument and the survey strategy. The main uncertainty is the fraction of neutral gas relative to the total dark matter within the object. We discuss this issue in the context of the observations which are currently available.Comment: 10 pages, 6 figure

Evidence of Gunn-Peterson damping wings in high-z quasar spectra: strengthening the case for incomplete reionization

The spectra of several high-redshift (z>6) quasars have shown evidence for a Gunn-Peterson (GP) damping wing, indicating a substantial mean neutral hydrogen fraction (x_HI > 0.03) in the z ~ 6 intergalactic medium (IGM). However, previous analyses assumed that the IGM was uniformly ionized outside of the quasar's HII region. Here we relax this assumption and model patchy reionization scenarios for a range of IGM and quasar parameters. We quantify the impact of these differences on the inferred x_HI, by fitting the spectra of three quasars: SDSS J1148+5251 (z=6.419), J1030+0524 (z=6.308), and J1623+3112 (z=6.247). We find that the best-fit values of x_HI in the patchy models agree well with the uniform case. More importantly, we confirm that the observed spectra favor the presence of a GP damping wing, with peak likelihoods decreasing by factors of > few - 10 when the spectra are modeled without a damping wing. We also find that the Ly alpha absorption spectra, by themselves, cannot distinguish the damping wing in a relatively neutral IGM from a damping wing in a highly ionized IGM, caused either by an isolated neutral patch, or by a damped Ly alpha absorber (DLA). However, neutral patches in a highly ionized universe (x_HI < 0.01), and DLAs with the large required column densities (N_HI > few x 10^{20} cm^{-2}) are both rare. As a result, when we include reasonable prior probabilities for the line of sight (LOS) to intercept either a neutral patch or a DLA at the required distance of ~ 40-60 comoving Mpc away from the quasar, we find strong lower limits on the neutral fraction in the IGM, x_HI > 0.1 (at 95% confidence). This strengthens earlier claims that a substantial global fraction of hydrogen in the z~6 IGM is in neutral form.Comment: 18 pages, 7 figures, version accepted for publication in the MNRA

‘A nation of town criers’: civic publicity and historical pageantry in inter-war Britain

Historical pageantry emerged in 1905 as the brainchild of the theatrical impresario Louis Napoleon Parker. Large casts of volunteers re-enacted successive scenes of local history, as crowds of thousands watched on, in large outdoor arenas. As the press put it, Britain had caught ‘pageant fever’. Towards the end of the 1920s, there was another outburst of historical pageantry. Yet, in contrast to the Edwardian period, when pageants took place in small towns, this revival was particularly vibrant in large industrial towns and cities. This article traces the popularity of urban pageantry to an inter-war ‘civic publicity’ movement. In doing so, it reassesses questions of local cultural decline; the role of local government; and the relationship of civic responsibility to popular theatre

Fundamental Parameters and Abundances of Metal-Poor Stars: The SDSS Standard BD +17 4708

The atmospheric parameters and iron abundance of the Sloan Digital Sky Survey (SDSS) spectrophotometric standard star BD +17 4708 are critically examined using up-to-date Kurucz model atmospheres, LTE line formation calculations, and reliable atomic data. We find Teff = 6141+-50 K, log g = 3.87+-0.08, and [Fe/H]=-1.74+-0.09. The line-of-sight interstellar reddening, bolometric flux, limb-darkened angular diameter, stellar mass, and the abundances of Mg, Si, and Ca are also obtained. This star is a unique example of a moderately metal-poor star for which the effective temperature can be accurately constrained from the observed spectral energy distribution (corrected for reddening). Such analysis leads to a value that is higher than most spectroscopic results previously reported in the literature (~5950 K). We find that the ionization balance of Fe lines is satisfied only if a low Teff (~5950 K) is adopted. With our preferred Teff (6141 K), the mean iron abundance we obtain from the FeII lines is lower by about 0.15 dex than that from the FeI lines, and therefore, the discrepancy between the mean iron abundance from FeI and FeII lines cannot be explained by overionization by UV photons as the main non-LTE effect. We also comment on non-LTE effects and the importance of inelastic collisions with neutral H atoms in the determination of oxygen abundances in metal-poor stars from the 777 nm OI triplet. (Abridged)Comment: A&A in pres

A Mismatch in the Ultraviolet Spectra between Low-Redshift and Intermediate-Redshift Type Ia Supernovae as a Possible Systematic Uncertainty for Supernova Cosmology

We present Keck high-quality rest-frame ultraviolet (UV) through optical spectra of 21 Type Ia supernovae (SNe Ia) in the redshift range 0.11 < z < 0.37 and a mean redshift of 0.22 that were discovered during the Sloan Digital Sky Survey-II (SDSS-II) SN Survey. Using the broad-band photometry of the SDSS survey, we are able to reconstruct the SN host-galaxy spectral energy distributions (SEDs), allowing for a correction for the host-galaxy contamination in the SN Ia spectra. Comparison of composite spectra constructed from a subsample of 17 high-quality spectra to those created from a low-redshift sample with otherwise similar properties shows that the Keck/SDSS SNe Ia have, on average, extremely similar rest-frame optical spectra but show a UV flux excess. This observation is confirmed by comparing synthesized broad-band colors of the individual spectra, showing a difference in mean colors at the 2.4 - 4.4 sigma level for various UV colors. We further see a slight difference in the UV spectral shape between SNe with low-mass and high-mass host galaxies. Additionally, we detect a relationship between the flux ratio at 2770 and 2900 A and peak luminosity that differs from that observed at low redshift. We find that changing the UV SED of an SN Ia within the observed dispersion can change the inferred distance moduli by ~0.1 mag. This effect only occurs when the data probe the rest-frame UV. We suggest that this discrepancy could be due to differences in the host-galaxy population of the two SN samples or to small-sample statistics.Comment: 28 pages, 21 figures, accepted by AJ, spectra are available at http://www.cfa.harvard.edu/~rfoley/data