Alien Registration- Doucette, Daniel A. (Portland, Cumberland County)

https://digitalmaine.com/alien_docs/24039/thumbnail.jp

Alien Registration- Doucette, Daniel A. (Portland, Cumberland County)

https://digitalmaine.com/alien_docs/24039/thumbnail.jp

Alien Registration- Doucette, Daniel A. (Portland, Cumberland County)

https://digitalmaine.com/alien_docs/24039/thumbnail.jp

Nationalism and Patriotic Idealism as Thematic Aspects in Art

This thesis has a two-fold purpose: (1) to provide an historical outline of the incidence of patriotic thematic material in Western art by presenting examples of works from dominant Western cultures beginning with Greece and Rome and including Britain, France, Germany, Spain, Italy and the United States. Examples given are in sequence from the eleventh century to the present excluding the twelfth, thirteenth, and fourteenth centuries. Greek and Roman examples are from two major epochs—the Hellenistic period of Greece, 323 to 146 B.C. (Greek period of nationalism) and the period 27 to 117 A.D. in Roman history (the period in which Rome achieved great power and prosperity). The outline is limited in the sense that each culture or time period is not covered in depth; an attempt is not made to trace influences in style or techniques or to give an extensive critique on the works. Rather, by the presentations of the examples it is intended to demonstrate that patriotic imagery does exist in art throughout history, it does exist in cultures of Western man, and by the nature of the content in the works (and through our knowledge of history and the artists) it does present a culture\u27s viewpoint toward national imagery (in some cases viewpoints of segments of the population and in other cases viewpoints of a large majority). Examples given were chosen on the basis of their status as major historical works and because of the extensive amount of national imagery employed in each. (2) To present a discussion of my personal artistic involvement with the patriotic theme and to present selected examples of my works in which national imagery has been employed. Discussion of my work is confined to art work produced in the period 1969-1971 and is intended to provide a comprehensive survey of work completed during this interval

The state of the Martian climate

60°N was +2.0°C, relative to the 1981–2010 average value (Fig. 5.1). This marks a new high for the record. The average annual surface air temperature (SAT) anomaly for 2016 for land stations north of starting in 1900, and is a significant increase over the previous highest value of +1.2°C, which was observed in 2007, 2011, and 2015. Average global annual temperatures also showed record values in 2015 and 2016. Currently, the Arctic is warming at more than twice the rate of lower latitudes

Development of an amplicon-based sequencing approach in response to the global emergence of mpox

The 2022 multicountry mpox outbreak concurrent with the ongoing Coronavirus Disease 2019 (COVID-19) pandemic further highlighted the need for genomic surveillance and rapid pathogen whole-genome sequencing. While metagenomic sequencing approaches have been used to sequence many of the early mpox infections, these methods are resource intensive and require samples with high viral DNA concentrations. Given the atypical clinical presentation of cases associated with the outbreak and uncertainty regarding viral load across both the course of infection and anatomical body sites, there was an urgent need for a more sensitive and broadly applicable sequencing approach. Highly multiplexed amplicon-based sequencing (PrimalSeq) was initially developed for sequencing of Zika virus, and later adapted as the main sequencing approach for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Here, we used PrimalScheme to develop a primer scheme for human monkeypox virus that can be used with many sequencing and bioinformatics pipelines implemented in public health laboratories during the COVID-19 pandemic. We sequenced clinical specimens that tested presumptively positive for human monkeypox virus with amplicon-based and metagenomic sequencing approaches. We found notably higher genome coverage across the virus genome, with minimal amplicon drop-outs, in using the amplicon-based sequencing approach, particularly in higher PCR cycle threshold (Ct) (lower DNA titer) samples. Further testing demonstrated that Ct value correlated with the number of sequencing reads and influenced the percent genome coverage. To maximize genome coverage when resources are limited, we recommend selecting samples with a PCR Ct below 31 Ct and generating 1 million sequencing reads per sample. To support national and international public health genomic surveillance efforts, we sent out primer pool aliquots to 10 laboratories across the United States, United Kingdom, Brazil, and Portugal. These public health laboratories successfully implemented the human monkeypox virus primer scheme in various amplicon sequencing workflows and with different sample types across a range of Ct values. Thus, we show that amplicon-based sequencing can provide a rapidly deployable, cost-effective, and flexible approach to pathogen whole-genome sequencing in response to newly emerging pathogens. Importantly, through the implementation of our primer scheme into existing SARS-CoV-2 workflows and across a range of sample types and sequencing platforms, we further demonstrate the potential of this approach for rapid outbreak response.This publication was made possible by CTSA Grant Number UL1 TR001863 from the National Center for Advancing Translational Science (NCATS), a component of the National Institutes of Health (NIH) awarded to CBFV. INSA was partially funded by the HERA project (Grant/ 2021/PHF/23776) supported by the European Commission through the European Centre for Disease Control (to VB).info:eu-repo/semantics/publishedVersio

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Alien Registration- Doucette, Daniel A. (Portland, Cumberland County)

https://digitalmaine.com/alien_docs/24039/thumbnail.jp

Alien Registration- Doucette, Daniel A. (Portland, Cumberland County)

https://digitalmaine.com/alien_docs/24039/thumbnail.jp