Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018.

Over the past decade, the Nomenclature Committee on Cell Death (NCCD) has formulated guidelines for the definition and interpretation of cell death from morphological, biochemical, and functional perspectives. Since the field continues to expand and novel mechanisms that orchestrate multiple cell death pathways are unveiled, we propose an updated classification of cell death subroutines focusing on mechanistic and essential (as opposed to correlative and dispensable) aspects of the process. As we provide molecularly oriented definitions of terms including intrinsic apoptosis, extrinsic apoptosis, mitochondrial permeability transition (MPT)-driven necrosis, necroptosis, ferroptosis, pyroptosis, parthanatos, entotic cell death, NETotic cell death, lysosome-dependent cell death, autophagy-dependent cell death, immunogenic cell death, cellular senescence, and mitotic catastrophe, we discuss the utility of neologisms that refer to highly specialized instances of these processes. The mission of the NCCD is to provide a widely accepted nomenclature on cell death in support of the continued development of the field

EVALUATING THE IMPACTS OF INPUT AND PARAMETER UNCERTAINTY ON STREAMFLOW SIMULATIONS IN LARGE UNDER-INSTRUMENTED BASINS

In data-poor regions around the world, particularly in less-privileged countries, hydrologists cannot always take advantage of available hydrological models to simulate a hydrological system due to the lack of reliable measurements of hydrological variables, in particular rainfall and streamflows, needed to implement and evaluate these models. Rainfall estimates obtained with remotely deployed sensors constitute an excellent source of precipitation for these basins, however they are prone to errors that can potentially affect hydrologic simulations. Concurrently, limited access to streamflow measurements does not allow a detailed representation of the system's structure through parameter estimation techniques. This dissertation presents multiple studies that evaluate the usefulness of remotely sensed products for different hydrological applications and the sensitivity of simulated streamflow to parameter uncertainty across basins with different hydroclimatic characteristics with the ultimate goal of increasing the applicability of land surface models in ungauged basins, particularly in South America. Paper 1 presents a sensitivity analysis of daily simulated streamflows to changes in model parameters along a hydroclimatic gradient. Parameters controlling the generation of surface and subsurface flow were targeted for the study. Results indicate that the sensitivity is strongly controlled by climate and that a more parsimonious version of the model could be implemented. Paper 2 explores how errors in satellite-estimated precipitation, due to infrequent satellite measurements, propagate through the simulation of a basin's hydrological cycle and impact the characteristics of peak streamflows within the basin. Findings indicate that nonlinearities in the hydrological cycle can introduce bias in simulated streamflows with error-corrupted precipitation. They also show that some characteristics of peak discharges are not conditioned by errors in satellite-estimated precipitation at a daily time step. Paper 3 evaluates the dominant sources of error in three satellite products when representing convective storms and how shifts in the location of the storm affect simulated peak streamflows in the basin. Results indicate that satellite products show some deficiencies retrieving convective processes and that a ground bias correction can mitigate these deficiencies but without sacrificing the potential for real-time hydrological applications. Finally, spatially shifted precipitation fields affect the magnitude of the peaks, however, its impact on the timing of the peaks is dampened out by the system's response at a daily time scale.Release after 2/16/201

Seasonal and Spatial Variations of PM10 and PM2.5 Oxidative Potential in Five Urban and Rural Sites across Lombardia Region, Italy

Oxidative potential (OP) of particulate matter (PM) is gaining strong interest as a promising health exposure metric. This study investigated OP of a large set of PM10 and PM2.5 samples collected at five urban and background sites near Milan (Italy), one of the largest and most polluted urban areas in Europe, afflicted with high particle levels. OP responses from two acellular assays, based on ascorbic acid (AA) and dithiothreitol (DTT), were combined with atmospheric detailed composition to examine any possible feature in OP with PM size fraction, spatial and seasonal variations. A general association of volume-normalized OP with PM mass was found; this association may be related to the clear seasonality observed, whereby there was higher OP activity in wintertime at all investigated sites. Univariate correlations were used to link OP with the concentrations of the major chemical markers of vehicular and biomass burning emissions. Of the two assays, AA was particularly sensitive towards transition metals in coarse particles released from vehicular traffic. The results obtained confirm that the responses from the two assays and their relationship with atmospheric pollutants are assay- and location-dependent, and that their combination is therefore helpful to singling out the PM redox-active compounds driving its oxidative properties

Survey of neonatal respiratory care and surfactant administration in very preterm infants in the Italian neonatal network

Introduction: Variation of respiratory care is described between centers around the world.The Italian Neonatal Network (INN), as a national group of the Vermont-Oxford Network (VON) allows to perform a wide analysis of respiratory care in very low birth weight infants. Methods:We analyzed the dataset of infants enrolled in the INN in 2009 and 2010 and, for surfactant administration only, from 2006 to 2010 from 83 participating centers. All definitions are those of the (VON). A questionnaire analysis was also performed with a questionnaire on centers practices. Results: We report data for 8297 infants. Data on ventilator practices and outcomes are outlined. Variation for both practices and outcome is found. Trend in surfactant administration is also analyzed. Conclusions. The great variation across hospitals in all the surveyed techniques points to the possibility of implementing potentially better practices with the aim of reducing unwanted variation. These data also show the power of large neonatal networks in identifying areas for potential improvement. \ua9 Mattioli 1885

Survey of neonatal respiratory care and surfactant administration in very preterm infants in the Italian neonatal network

Introduction: Variation of respiratory care is described between centers around the world.The Italian Neonatal Network (INN), as a national group of the Vermont-Oxford Network (VON) allows to perform a wide analysis of respiratory care in very low birth weight infants. Methods:We analyzed the dataset of infants enrolled in the INN in 2009 and 2010 and, for surfactant administration only, from 2006 to 2010 from 83 participating centers. All definitions are those of the (VON). A questionnaire analysis was also performed with a questionnaire on centers practices. Results: We report data for 8297 infants. Data on ventilator practices and outcomes are outlined. Variation for both practices and outcome is found. Trend in surfactant administration is also analyzed. Conclusions. The great variation across hospitals in all the surveyed techniques points to the possibility of implementing potentially better practices with the aim of reducing unwanted variation. These data also show the power of large neonatal networks in identifying areas for potential improvement. © Mattioli 1885