First evidence for a gravitational lensing-induced echo in gamma rays with Fermi LAT

Aims. This article shows the first evidence for gravitational lensing phenomena in high energy gamma-rays. This evidence comes from the observation of a gravitational lens induced echo in the light curve of the distant blazar PKS 1830-211. Methods. Traditional methods for the estimation of time delays in gravitational lensing systems rely on the cross-correlation of the light curves of the individual images. In this paper, we use 300 MeV-30 GeV photons detected by the Fermi-LAT instrument. The Fermi-LAT instrument cannot separate the images of known lenses. The observed light curve is thus the superposition of individual image light curves. The Fermi-LAT instrument has the advantage of providing long, evenly spaced, time series. In addition, the photon noise level is very low. This allows to use directly Fourier transform methods. Results. A time delay between the two compact images of PKS 1830-211 has been searched for both by the autocorrelation method and the "double power spectrum" method. The double power spectrum shows a 3 {\sigma} evidence for a time delay of 27.5 $\pm$ 1.3 days, consistent with the result from Lovell et al. (1998). The relative uncertainty on the time delay estimation is reduced from 20% to 5%.Comment: submitted to A&

Strong normal-incidence infrared absorption in self-organized InAs/InAlAs quantum dots grown on InP(001)

International audienceInAs self-assembled quantum dots in InAlAs matrix grown on InP001 substrates have been fabricated using Stranski-Krastanov growth mode. A strong in-plane polarized intraband absorption in the 10.6-20 m wavelength region has been observed and ascribed to a transition from the ground electron state to an excited state confined in the layer plane along the 110 direction. The absorption at normal-incidence reaches 7.8% for ten layers of n-doped quantum dots. The oscillator strength of the intraband transition is comparable to that achieved in quantum wells for a conduction band intersubband transition. The dependence of the intraband absorption on carrier concentration and temperature suggests a quantum-wire type confinement potential

Ringing effects reduction by improved deconvolution algorithm Application to A370 CFHT image of gravitational arcs

We develop a self-consistent automatic procedure to restore informations from astronomical observations. It relies on both a new deconvolution algorithm called LBCA (Lower Bound Constraint Algorithm) and the use of the Wiener filter. In order to explore its scientific potential for strong and weak gravitational lensing, we process a CFHT image of the galaxies cluster Abell 370 which exhibits spectacular strong gravitational lensing effects. A high quality restoration is here of particular interest to map the dark matter within the cluster. We show that the LBCA turns out specially efficient to reduce ringing effects introduced by classical deconvolution algorithms in images with a high background. The method allows us to make a blind detection of the radial arc and to recover morphological properties similar to thoseobserved from HST data. We also show that the Wiener filter is suitable to stop the iterative process before noise amplification, using only the unrestored data.Comment: A&A in press 9 pages 9 figure

Plasma membrane-associated receptor like kinases relocalize to plasmodesmata in response to osmotic stress

Plasmodesmata act as key elements in intercellular communication, coordinating processes related to plant growth, development, and responses to environmental stresses. While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata also cluster receptor-like activities; whether these two pathways interact is currently unknown. Here we show that specific PM-located Leucine-Rich-Repeat Receptor-Like-Kinases (LRR-RLKs), QSK1 and IMK2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress. This process is remarkably fast, is not a general feature of PM-associated proteins, and is independent of sterol- and sphingolipid- membrane composition. Focusing on QSK1, previously reported to be involved in stress responses, we show that relocalisation in response to mannitol depends on QSK1 phosphorylation. Loss-of-function mutation in QSK1 results in delayed lateral root (LR) development and the mutant is affected in the root response to mannitol stress. Callose-mediated plasmodesmata regulation is known to regulate LR development. We found that callose levels are reduced in the qsk1 mutant background with a root phenotype resembling ectopic expression of PdBG1, an enzyme that degrades callose at the pores. Both the LR and callose phenotypes can be complemented by expression of wild-type and phosphomimic QSK1 variants, but not by phosphodead QSK1 mutant which fails to relocalise at plasmodesmata. Together the data indicate that re-organisation of RLKs to plasmodesmata is important for the regulation of callose and LR development as part of the plant response to osmotic stress

A lack of close binaries among hot horizontal branch stars in globular clusters. II. NGC\,2808

Models based on their binary origin have been very successful in reproducing the properties of field subdwarf-B stars, but the observations of their analogues in globular clusters has posed new problems, while the discovery of multiple populations offered an appealing alternative scenario for the formation of these stars. We search for binaries of period P<200 days among a sample of blue horizontal branch stars (Teff=12000-22000 K) in NGC2808, a cluster known to host three distinct stellar populations and a multimodal horizontal branch. The final sample consists of 64 targets. The radial velocity of the targets was measured in fourteen epochs, spanning a temporal interval of about 75 days. We detect no RV variable object among stars cooler than the photometric G1 gap at 17000 K, while two close (P<10 days) and two intermediate-period (P=10-50 days) systems are found among hotter targets. The close and intermediate-period binary fraction for stars cooler than the gap are fc<5% and fip<10%, respectively, with 95% confidence. The most probable values among hotter stars are fc~20% and fip~30%, but the 90%- confidence level intervals are large (6-42% and 11-72%, respectively). The G1 gap appears as a discontinuity in the binary faction, with a higher incidence of binaries among hotter stars, but a constant increase in f with temperature rather than a discontinuity cannot be excluded from our observations. We find that intermediate-period binaries, never investigated before among cluster HB stars, could play an important role, being more than ~15-20% of the hottest stars of our sample. Our results indicate that fc among hot HB stars is most probably higher for younger clusters, confirming the recently proposed age-fc relation. However, the large observed difference in binary fraction between clusters (e.g. NGC2808 and NGC6752) is still not reproduced by binary population synthesis models

AUX1-mediated root hair auxin influx governs SCFTIR1/AFB-type Ca2+ signaling

Auxin is a key regulator of plant growth and development, but the causal relationship between hormone transport and root responses remains unresolved. Here we describe auxin uptake, together with early steps in signaling, in Arabidopsis root hairs. Using intracellular microelectrodes we show membrane depolarization, in response to IAA in a concentration- and pH-dependent manner. This depolarization is strongly impaired in aux1 mutants, indicating that AUX1 is the major transporter for auxin uptake in root hairs. Local intracellular auxin application triggers Ca2+ signals that propagate as long-distance waves between root cells and modulate their auxin responses. AUX1-mediated IAA transport, as well as IAA- triggered calcium signals, are blocked by treatment with the SCFTIR1/AFB - inhibitor auxinole. Further, they are strongly reduced in the tir1afb2afb3 and the cngc14 mutant. Our study reveals that the AUX1 transporter, the SCFTIR1/AFB receptor and the CNGC14 Ca2+ channel, mediate fast auxin signaling in roots

Comparison of Argentinean Saint Louis Encephalitis Virus Non-Epidemic and Epidemic Strain Infections in an Avian Model

St. Louis encephalitis virus (SLEV, Flavivirus, Flaviviridae) is an emerging mosquito-borne pathogen in South America, with human SLEV encephalitis cases reported in Argentina and Brazil. Genotype III strains of SLEV were isolated from Culex quinquefasciatus mosquitoes in Cordoba, Argentina in 2005, during the largest SLEV outbreak ever reported in South America. The present study tested the hypothesis that the recent, epidemic SLEV strain exhibits greater virulence in birds as compared with a non-epidemic genotype III strain isolated from mosquitoes in Santa Fe Province 27 years earlier. The observed differences in infection parameters between adult House sparrows (Passer domesticus) that were needle-inoculated with either the epidemic or historic SLEV strain were not statistically significant. However, only the House sparrows that were infected with the epidemic strain achieved infectious-level viremia titers sufficient to infect Cx. spp. mosquitoes vectors. Furthermore, the vertebrate reservoir competence index values indicated an approximately 3-fold increase in amplification potential of House sparrows infected with the epidemic strain when pre-existing flavivirus-reactive antibodies were present, suggesting the possibility that antibody-dependent enhancement may increase the risk of avian-amplified transmission of SLEV in South America

How a Diverse Research Ecosystem Has Generated New Rehabilitation Technologies: Review of NIDILRR’s Rehabilitation Engineering Research Centers

Over 50 million United States citizens (1 in 6 people in the US) have a developmental, acquired, or degenerative disability. The average US citizen can expect to live 20% of his or her life with a disability. Rehabilitation technologies play a major role in improving the quality of life for people with a disability, yet widespread and highly challenging needs remain. Within the US, a major effort aimed at the creation and evaluation of rehabilitation technology has been the Rehabilitation Engineering Research Centers (RERCs) sponsored by the National Institute on Disability, Independent Living, and Rehabilitation Research. As envisioned at their conception by a panel of the National Academy of Science in 1970, these centers were intended to take a “total approach to rehabilitation”, combining medicine, engineering, and related science, to improve the quality of life of individuals with a disability. Here, we review the scope, achievements, and ongoing projects of an unbiased sample of 19 currently active or recently terminated RERCs. Specifically, for each center, we briefly explain the needs it targets, summarize key historical advances, identify emerging innovations, and consider future directions. Our assessment from this review is that the RERC program indeed involves a multidisciplinary approach, with 36 professional fields involved, although 70% of research and development staff are in engineering fields, 23% in clinical fields, and only 7% in basic science fields; significantly, 11% of the professional staff have a disability related to their research. We observe that the RERC program has substantially diversified the scope of its work since the 1970’s, addressing more types of disabilities using more technologies, and, in particular, often now focusing on information technologies. RERC work also now often views users as integrated into an interdependent society through technologies that both people with and without disabilities co-use (such as the internet, wireless communication, and architecture). In addition, RERC research has evolved to view users as able at improving outcomes through learning, exercise, and plasticity (rather than being static), which can be optimally timed. We provide examples of rehabilitation technology innovation produced by the RERCs that illustrate this increasingly diversifying scope and evolving perspective. We conclude by discussing growth opportunities and possible future directions of the RERC program

Clinical trials in Charcot-Marie-Tooth disorders: a retrospective and preclinical assessment

ObjectiveThis study aimed to evaluate the progression of clinical and preclinical trials in Charcot-Marie-Tooth (CMT) disorders.BackgroundCMT has historically been managed symptomatically and with genetic counseling. The evolution of molecular and pathologic understanding holds a therapeutic promise in gene-targeted therapies.MethodsClinicalTrials.gov from December 1999 to June 2022 was data extracted for CMT with preclinical animal gene therapy trials also reviewed by PubMed search.ResultsThe number of active trials was 1 in 1999 and 286 in 2022. Academic settings accounted for 91% and pharmaceutical companies 9%. Of the pharmaceutical and academic trials, 38% and 28%, respectively, were controlled, randomized, and double-blinded. Thirty-two countries participated: the United States accounted for 26% (75/286). In total, 86% of the trials were classified as therapeutic: 50% procedural (21% wrist/elbow surgery; 22% shock wave and hydrodissection therapy), 23% investigational drugs, 15% devices, and 11% physical therapy. Sixty-seven therapeutic trials (49%) were designated phases 1–2 and 51% phases 3–4. The remaining 14% represent non-therapeutic trials: diagnostic testing (3%), functional outcomes (4%), natural history (4%), and standard of care (3%). One-hundred and three (36%) resulted in publications. Phase I human pharmaceutical trials are focusing on the safety of small molecule therapies (n = 8) and AAV and non-viral gene therapy (n = 3). Preclinical animal gene therapy studies include 11 different CMT forms including viral, CRISPR-Cas9, and nanoparticle delivery.ConclusionCurrent CMT trials are exploring procedural and molecular therapeutic options with substantial participation of the pharmaceutical industry worldwide. Emerging drug therapies directed at molecular pathogenesis are being advanced in human clinical trials; however, the majority remain within animal investigations

Production of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular expression of Saccharomyces cerevisiae acyl-CoA:ethanol O-acyltransferase Eht1 or Eeb1

Medium-chain volatile flavour esters are important molecules since they have extensive applications in food, fragrance, cosmetic, paint and coating industries, which determine different characteristics of aroma or taste in commercial products. Biosynthesis of these compounds by alcoholysis is catalyzed by acyl-CoA:ethanol O-acyltransferases Eht1 or Eeb1 in Saccharomyces cerevisiae. In this study, these two yeast enzymes were selected to explore their preparations as the form of whole cell biocatalysts for the production of volatile flavour esters. Here, the novel whole cell biocatalysts Pichia pastoris yeasts with functional extracellular expression of Eht1 or Eeb1 were constructed. Flavour production was established through an integrated process with coupled enzyme formation and ester biosynthesis in the recombinant yeasts in one pot, leading to the formation of volatile C6–C14 methyl and ethyl esters from wort medium. Interestingly, there is no significant difference between P. pastoris-EHT1 and P. pastoris-EEB1 in substrate preference during flavour biosynthesis, indicating a similar role of Eht1 and Eeb1 in P. pastoris cells, in contradiction with previous findings in S. cerevisiae to some extent. Consequently the study not only provides a greater understanding of these two enzymes in a heterogeneous host, but also demonstrated the positive effect of the recombinant Eht1 and Eeb1 in ester formation by P. pastoris live cells, potentially paving the way towards achieving efficient production of volatile flavour by an integrated biocatalytic system composed of recombinant enzyme production and flavour biosynthesis