Early Alteration of Nucleocytoplasmic Traffic Induced by Some RNA Viruses

AbstractA HeLa cell line expressing the green fluorescent protein fused to the SV40 T-antigen nuclear localization signal (EGFP-NLS) was established. Fluorescence in these cells was confined to the nuclei. After poliovirus infection, cytoplasmic fluorescence in a proportion of cells could be detected by 1 h postinfection (p.i.) and in virtually all of the fluorescent cells by 2 h p.i. The relocation could be prevented by cycloheximide but not by inhibition of poliovirus replication by guanidine · HCl. Nuclear exit of a protein composed of three copies of GFP fused to the NLS also occurred upon poliovirus infection. A similar redistribution of EGFP-NLS took place upon infection with coxsakievirus B3 and, to a lesser extent, with vesicular stomatitis virus. The EGFP-NLS efflux was not due to the loss of NLS. Thus, some positive-strand and negative-strand RNA viruses trigger a rapid nonspecific relocation of nuclear proteins

Prothymosin α fragmentation in apoptosis

AbstractWe observed fragmentation of an essential proliferation-related human nuclear protein prothymosin α in the course of apoptosis induced by various stimuli. Prothymosin α cleavage occurred at the DDVD99 motif. In vitro, prothymosin α could be cleaved at D99 by caspase-3 and -7. Caspase hydrolysis disrupted the nuclear localization signal of prothymosin α and abrogated the ability of the truncated protein to accumulate inside the nucleus. Prothymosin α fragmentation may therefore be proposed to disable intranuclear proliferation-related function of prothymosin α in two ways: by cleaving off a short peptide containing important determinants, and by preventing active nuclear uptake of the truncated protein

Methane in the atmosphere of the transiting hot Neptune GJ436b?

We present an analysis of seven primary transit observations of the hot Neptune GJ436b at 3.6, 4.5 and $8~\mu$m obtained with the Infrared Array Camera (IRAC) on the Spitzer Space Telescope. After correcting for systematic effects, we fitted the light curves using the Markov Chain Monte Carlo technique. Combining these new data with the EPOXI, HST and ground-based $V, I, H$ and $K_s$ published observations, the range $0.5-10~\mu$m can be covered. Due to the low level of activity of GJ436, the effect of starspots on the combination of transits at different epochs is negligible at the accuracy of the dataset. Representative climate models were calculated by using a three-dimensional, pseudo-spectral general circulation model with idealised thermal forcing. Simulated transit spectra of GJ436b were generated using line-by-line radiative transfer models including the opacities of the molecular species expected to be present in such a planetary atmosphere. A new, ab-initio calculated, linelist for hot ammonia has been used for the first time. The photometric data observed at multiple wavelengths can be interpreted with methane being the dominant absorption after molecular hydrogen, possibly with minor contributions from ammonia, water and other molecules. No clear evidence of carbon monoxide and dioxide is found from transit photometry. We discuss this result in the light of a recent paper where photochemical disequilibrium is hypothesised to interpret secondary transit photometric data. We show that the emission photometric data are not incompatible with the presence of abundant methane, but further spectroscopic data are desirable to confirm this scenario.Comment: 19 pages, 10 figures, 1 table, Astrophysical Journal in pres

Spitzer Secondary Eclipse Observations of Five Cool Gas Giant Planets and Empirical Trends in Cool Planet Emission Spectra

In this work we present Spitzer 3.6 and 4.5 micron secondary eclipse observations of five new cool (<1200 K) transiting gas giant planets: HAT-P-19b, WASP-6b, WASP-10b, WASP-39b, and WASP-67b. We compare our measured eclipse depths to the predictions of a suite of atmosphere models and to eclipse depths for planets with previously published observations in order to constrain the temperature- and mass-dependent properties of gas giant planet atmospheres. We find that the dayside emission spectra of planets less massive than Jupiter require models with efficient circulation of energy to the night side and/or increased albedos, while those with masses greater than that of Jupiter are consistently best-matched by models with inefficient circulation and low albedos. At these relatively low temperatures we expect the atmospheric methane to CO ratio to vary as a function of metallicity, and we therefore use our observations of these planets to constrain their atmospheric metallicities. We find that the most massive planets have dayside emission spectra that are best-matched by solar metallicity atmosphere models, but we are not able to place strong constraints on metallicities of the smaller planets in our sample. Interestingly, we find that the ratio of the 3.6 and 4.5 micron brightness temperatures for these cool transiting planets is independent of planet temperature, and instead exhibits a tentative correlation with planet mass. If this trend can be confirmed, it would suggest that the shape of these planets' emission spectra depends primarily on their masses, consistent with the hypothesis that lower-mass planets are more likely to have metal-rich atmospheres.Comment: 16 pages, 14 figures, accepted for publication in Ap

Can we measure the accretion efficiency of Active Galactic Nuclei?

The accretion efficiency for individual black holes is very difficult to determine accurately. There are many factors that can influence each step of the calculation, such as the dust and host galaxy contribution to the observed luminosity, the black hole mass and more importantly, the uncertainties on the bolometric luminosity measurement. Ideally, we would measure the AGN emission at every wavelength, remove the host galaxy and dust, reconstruct the AGN spectral energy distribution and integrate to determine the intrinsic emission and the accretion rate. In reality, this is not possible due to observational limitations and our own galaxy line of sight obscuration. We have then to infer the bolometric luminosity from spectral measurements made in discontinuous wavebands and at different epochs. In this paper we tackle this issue by exploring different methods to determine the bolometric luminosity. We first explore the trend of accretion efficiency with black hole mass (efficiency proportional to M^{\sim 0.5}) found in recent work by Davis & Laor and discuss why this is most likely an artefact of the parameter space covered by their PG quasar sample. We then target small samples of AGN at different redshifts, luminosities and black hole masses to investigate the possible methods to calculate the accretion efficiency. For these sources we are able to determine the mass accretion rate and, with some assumptions, the accretion efficiency distributions. Even though we select the sources for which we are able to determine the parameters more accurately, there are still factors affecting the measurements that are hard to constrain. We suggest methods to overcome these problems based on contemporaneous multi-wavelength data measurements and specifically targeted observations for AGN in different black hole mass ranges.Comment: 16 pages, 20 figures, accepted for publication in MNRA

Non-LTE Models and Theoretical Spectra of Accretion Disks in Active Galactic Nuclei. IV. Effects of Compton Scattering and Metal Opacities

We extend our models of the vertical structure and emergent radiation field of accretion disks around supermassive black holes described in previous papers of this series. Our models now include both a self-consistent treatment of Compton scattering and the effects of continuum opacities of the most important metal species (C, N, O, Ne, Mg, Si, S, Ar, Ca, Fe, Ni). With these new effects incorporated, we compute the predicted spectrum from black holes accreting at nearly the Eddington luminosity (L/L_Edd = 0.3) and central masses of 10^6, 10^7, and 10^8 M_sun. We also consider two values of the Shakura-Sunyaev alpha parameter, 0.1 and 0.01. Although it has little effect when M > 10^8 M_sun, Comptonization grows in importance as the central mass decreases and the central temperature rises. It generally produces an increase in temperature with height in the uppermost layers of hot atmospheres. Compared to models with coherent electron scattering, Comptonized models have enhanced EUV/soft X-ray emission, but they also have a more sharply declining spectrum at very high frequencies. Comptonization also smears the hydrogen and the He II Lyman edges. The effects of metals on the overall spectral energy distribution are smaller than the effects of Comptonization for these parameters. Compared to pure hydrogen-helium models, models with metal continuum opacities have reduced flux in the high frequency tail, except at the highest frequencies, where the flux is very low. Metal photoionization edges are not present in the overall disk-integrated model spectra. In addition to our new grid of models, we also present a simple analytic prescription for the vertical temperature structure of the disk in the presence of Comptonization, and show under what conditions a hot outer layer (a corona) is formed.Comment: 22 pages, Latex. 19 figures. Uses corrected version of emulateapj.sty and apjfonts.sty (included). Accepted for publication in Ap

Mapping Exoplanets

The varied surfaces and atmospheres of planets make them interesting places to live, explore, and study from afar. Unfortunately, the great distance to exoplanets makes it impossible to resolve their disk with current or near-term technology. It is still possible, however, to deduce spatial inhomogeneities in exoplanets provided that different regions are visible at different times---this can be due to rotation, orbital motion, and occultations by a star, planet, or moon. Astronomers have so far constructed maps of thermal emission and albedo for short period giant planets. These maps constrain atmospheric dynamics and cloud patterns in exotic atmospheres. In the future, exo-cartography could yield surface maps of terrestrial planets, hinting at the geophysical and geochemical processes that shape them.Comment: Updated chapter for Handbook of Exoplanets, eds. Deeg & Belmonte. 17 pages, including 6 figures and 4 pages of reference

Theory of disk accretion onto supermassive black holes

Accretion onto supermassive black holes produces both the dramatic phenomena associated with active galactic nuclei and the underwhelming displays seen in the Galactic Center and most other nearby galaxies. I review selected aspects of the current theoretical understanding of black hole accretion, emphasizing the role of magnetohydrodynamic turbulence and gravitational instabilities in driving the actual accretion and the importance of the efficacy of cooling in determining the structure and observational appearance of the accretion flow. Ongoing investigations into the dynamics of the plunging region, the origin of variability in the accretion process, and the evolution of warped, twisted, or eccentric disks are summarized.Comment: Mostly introductory review, to appear in "Supermassive black holes in the distant Universe", ed. A.J. Barger, Kluwer Academic Publishers, in pres

Magnetic fields of active galactic nuclei and quasars with polarized broad H-alpha lines

We present estimates of magnetic field in a number of AGNs from the Spectropolarimetric atlas of Smith, Young & Robinson (2002) from the observed degrees of linear polarization and the positional angles of spectral lines (H-alpha) (broad line regions of AGNs) and nearby continuum. The observed polarization is lower than the Milne value in a non-magnetized atmosphere. We hypothesize that the polarized radiation escapes from optically thick magnetized accretion discs and is weakened by the Faraday rotation effect. This effect is able to explain both the value of the polarization and the position angle. We estimate the required magnetic field in the broad line region by using simple asymptotic analytical formulas for Milne's problem in magnetized atmosphere, which take into account the last scattering of radiation before escaping from the accretion disc. The polarization of a broad spectral line escaping from disc is described by the same mechanism. The characteristic features of polarization of a broad line is the minimum of the degree of polarization in the center of the line and continuous rotation of the position angle from one wing to another. These effects can be explained by existence of clouds in the left (velocity is directed to an observer) and the right (velocity is directed from an observer) parts of the orbit in a rotating keplerian magnetized accretion disc. The base of explanation is existence of azimuthal magnetic field in the orbit. The existence of normal component of magnetic field makes the picture of polarization asymmetric. The existence of clouds in left and right parts of the orbit with different emissions also give the contribution in asymmetry effect. Assuming a power-law dependence of the magnetic field inside the disc, we obtain the estimate of the magnetic field strength at first stable orbit near the central SMBH for a number of AGNs.Comment: 15 pages, 4 figure

Characterising the Atmospheres of Transiting Planets with a Dedicated Space Telescope

Exoplanetary science is one of the fastest evolving fields of today's astronomical research, continuously yielding unexpected and surprising results. Ground-based planet-hunting surveys, together with dedicated space missions such as Kepler and CoRoT, are delivering an ever-increasing number of exoplanets, over 690, and ESA's Gaia mission will escalate the exoplanetary census into the several thousands. The next logical step is the characterization of these new worlds. What is their nature? Why are they as they are? Use of the Hubble Space Telescope and Spitzer Space Telescope to probe the atmospheres of transiting hot, gaseous exoplanets has opened perspectives unimaginable even just 10 years ago, demonstrating that it is indeed possible with current technology to address the ambitious goal of characterizing the atmospheres of these alien worlds. However, these successful measurements have also shown the difficulty of understanding the physics and chemistry of these exotic environments when having to rely on a limited number of observations performed on a handful of objects. To progress substantially in this field, a dedicated facility for exoplanet characterization, able to observe a statistically significant number of planets over time and a broad spectral range will be essential. Additionally, the instrument design (e.g., detector performances, photometric stability) will be tailored to optimize the extraction of the astrophysical signal. In this paper, we analyze the performance and tradeoffs of a 1.2/1.4 m space telescope for exoplanet transit spectroscopy from the visible to the mid-IR. We present the signal-to-noise ratio as a function of integration time and stellar magnitude/spectral type for the acquisition of spectra of planetary atmospheres for a variety of scenarios: hot, warm, and temperate planets orbiting stars ranging in spectral type from hot F- to cooler M-dwarfs. Our results include key examples of known planets (e.g., HD 189733b, GJ 436b, GJ 1214b, and Cancri 55 e) and simulations of plausible terrestrial and gaseous planets, with a variety of thermodynamical conditions. We conclude that even most challenging targets, such as super-Earths in the habitable zone of late-type stars, are within reach of an M-class, space-based spectroscopy mission