Endosomal interactions during root hair growth

The dynamic localization of endosomal compartments labeled with targeted fluorescent protein tags is routinely followed by time lapse fluorescence microscopy approaches and single particle tracking algorithms. In this way trajectories of individual endosomes can be mapped and linked to physiological processes as cell growth. However, other aspects of dynamic behavior including endosomal interactions are difficult to follow in this manner. Therefore, we characterized the localization and dynamic properties of early and late endosomes throughout the entire course of root hair formation by means of spinning disc time lapse imaging and post-acquisition automated multitracking and quantitative analysis. Our results show differential motile behavior of early and late endosomes and interactions of late endosomes that may be specified to particular root hair domains. Detailed data analysis revealed a particular transient interaction between late endosomes—termed herein as dancing-endosomes—which is not concluding to vesicular fusion. Endosomes preferentially located in the root hair tip interacted as dancing-endosomes and traveled short distances during this interaction. Finally, sizes of early and late endosomes were addressed by means of super-resolution structured illumination microscopy (SIM) to corroborate measurements on the spinning disc. This is a first study providing quantitative microscopic data on dynamic spatio-temporal interactions of endosomes during root hair tip growth

Immunolocalization of dually phosphorylated MAPKs in dividing root meristem cells of Vicia faba, Pisum sativum, Lupinus luteus and Lycopersicon esculentum

Key message In plants, phosphorylated MAPKs display constitutive nuclear localization; however, not all studied plant species show co-localization of activated MAPKs to mitotic microtubules. Abstract The mitogen-activated protein kinase (MAPK) signaling pathway is involved not only in the cellular response to biotic and abiotic stress but also in the regulation of cell cycle and plant development. The role of MAPKs in the formation of a mitotic spindle has been widely studied and the MAPK signaling pathway was found to be indispensable for the unperturbed course of cell division. Here we show cellular localization of activated MAPKs (dually phosphorylated at their TXY motifs) in both interphase and mitotic root meristem cells of Lupinus luteus, Pisum sativum, Vicia faba (Fabaceae) and Lycopersicon esculentum (Solanaceae). Nuclear localization of activated MAPKs has been found in all species. Colocalization of these kinases to mitotic microtubules was most evident in L. esculentum, while only about 50 % of mitotic cells in the root meristems of P. sativum and V. faba displayed activated MAPKs localized to microtubules during mitosis. Unexpectedly, no evident immunofluorescence signals at spindle microtubules and phragmoplast were noted in L. luteus. Considering immunocytochemical analyses and studies on the impact of FR180204 (an inhibitor of animal ERK1/2) on mitotic cells, we hypothesize that MAPKs may not play prominent role in the regulation of microtubule dynamics in all plant species

Liver-Targeting of Interferon-Alpha with Tissue-Specific Domain Antibodies

PMCID: PMC3581439This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Macro optical projection tomography for large scale 3D imaging of plant structures and gene activity

Optical projection tomography (OPT) is a well-established method for visualising gene activity in plants and animals. However, a limitation of conventional OPT is that the specimen upper size limit precludes its application to larger structures. To address this problem we constructed a macro version called Macro OPT (M-OPT). We apply M-OPT to 3D live imaging of gene activity in growing whole plants and to visualise structural morphology in large optically cleared plant and insect specimens up to 60 mm tall and 45 mm deep. We also show how M-OPT can be used to image gene expression domains in 3D within fixed tissue and to visualise gene activity in 3D in clones of growing young whole Arabidopsis plants. A further application of M-OPT is to visualise plant-insect interactions. Thus M-OPT provides an effective 3D imaging platform that allows the study of gene activity, internal plant structures and plant-insect interactions at a macroscopic scale

Pharmacokinetic Characteristics, Pharmacodynamic Effect and In Vivo Antiviral Efficacy of Liver-Targeted Interferon Alpha

Work carried out by JM was funded by the National Institutes of Health (NIH). NIH contract number is HHSN272201000039I/HHSN27200001/ A19

Extraction of unspecified relations from the web

The subject of this thesis is non-specific knowledge extraction from the web. In recent years, tools that improve the results of this type of knowledge extraction were created. The aim of this thesis is to become familiar with these tools, test and propose the use of results. In this thesis these tools are described and compared and extraction is carried out using OLLIE. Based on the results of the extractions, two methods of enriching extractions using name entity recognition, are proposed. The first method proposes to modify the weights of extractions and second proposes the enrichment of extractions by named entities. The paper proposed ontology, which allows to capture the structure of enriched extractions. In the last part practical experiment is carried out, in which the proposed methods are demonstrated. Future research in this field would be useful in areas of extraction and categorization of relational phrases

Comparison of chosen football clubs in Germany and Slovakia

katedra: KTV; rozsah: 62 s., 5 s. obr. přílohCieľom bakalárskej práce je pomocou získaných informácií porovnať a zistiť rozdiely dvoch futbalových klubov. Vybral som nemecký klub FSV Budissa Bautzen a slovenský klub TJ Bánová. Potrebné materiály a informácie som získal od prezidentov oboch klubov. Porovnaním bolo zistené, že kluby svojou organizačnou štruktúrou, veľkosťou, marketingovou činnosťou a štruktúrou rozpočtu sú veľmi odlišné. Práca klubu FSV Budissa Bautzen je kvalitnejšia. Záverečné porovnania obsahujú navrhnuté odporúčania, ktoré by mohli viesť k rozvoju klubov.Subject of this dissertation is comparison and identification of differences between two football clubs using available information. I have chosen the German club FSV Budissa Bautzen and Slovakian club TJ Bánová. I have obtained the necessary information from both club´s chairmen. Comparison has revealed that organizational structures, size, marketing activities and budget structures of both clubs are very different. The FSV Budissa Bautzen club operation is more advanced. Final comparison includes recommendations which could stimulate further development of both clubs