Learning Design at White Sands Test Facility

During the Fall of 2010, I spent my time at NASA White Sands Test Facility in Las Cruces, NM as an Undergraduate Student Research Program (USRP) Intern. During that time, I was given three projects to work on: Large Altitude Simulation System (LASS) basket strainer, log books, and the design of a case for touch screen monitors used for simulations. I spent most of my time on the LASS basket strainer. The LASS system has a water feed line with a basket strainer that filters out rust. In 2009, there were three misfires which cost approximately $27,000 and about 8% of the allotted time. The strainer was getting a large change in pressure that would result in a shutdown of the system. I have designed a new basket that will eliminate the large pressure change and it can be used with the old basket strainer housing. The LASS system has three steam generators (modules). Documents pertaining to these modules are stored electronically, and the majority of the documents are not able to be searched with keywords, so they have to be gone through one by one. I have come up with an idea on how to organize these files so that the Propulsion Department may efficiently search through the documents needed. Propulsion also has a LASS simulator that incorporates two touch screen monitors. Currently these monitors are in six foot by two foot metal cabinet on wheels. During simulation these monitors are used in the block house and need to be taken out of the block house when not in use. I have designed different options for hand held cases for storing and transporting the monitors in and out of the block house. The three projects previously mentioned demonstrate my contributions to the Propulsion Department and have taught me real world experience that is essential in becoming a productive engineer

unmentionables

This text explores the capacity for shamed bodily materiality to narrate the complexity of healing from sexual trauma while rape culture persists. Because rape is discussed so little in public, sexual healing often takes place under a meaty layer of shame, placed on the survivor’s body. Their truth is frequently interpreted as too much/gross/ugly/unspeakable for the public, and it is simultaneously not enough to be discussed/accepted/pursued as an actual issue. This uncomfortable teeter-totter comes from the patriarchal boundaries drawn between what is privately or publicly acceptable. There are plenty of depictions of sexual violence in popular culture and the canon of art hstory, but we rarely see examples of sexual healing. unmentionables is a heartfelt, raw, and painfully true compilation of my own endeavors to understand these implications as a survivor. Much of my references come from survivors who put words to what I felt was only expressible through sculpture. Dr. Bessel van der Kolk’s research on post-traumatic stress disorder, Julia Kristeva’s writings on the abject, and the phallocentric taboos placed on the vulva collectively provide context for why this is so. Social norms effectively shame what is beneath our skin, what we would rather not look at it but still know is there. This relates uncomfortably to subject of rape because of how the survivor is forced to carry the weight of the traumatic event, socially and physically. Thus the wet, goopy materiality of internal anatomy forms the skin of my sculptures as they embody the sensation of wearing shame. Inspired by Simone de Beauvoir’s writing on the body as dynamic, instead of passive, my sculptures blur the line between interior and exterior, and between private and public. This renders the survivor’s truth material and impossible to ignore. Content warning: sexual assaul

The roles of dispositional flow, dispositional mindfulness, and self-compassion in the Objectification Theory Framework

Title from PDF of title page, viewed on August 23, 2016Dissertation advisor: Jacob M. MarszalekVitaIncludes bibliographical references (pages 202-229)Thesis (Ph.D.)--School of Education. University of Missouri--Kansas City, 2016Women are at greater risk than men for experiencing eating disorders, depression, and sexual dysfunction (American Psychological Association, 2007; Fredrickson & Roberts, 1997). Objectification theory (Fredrickson & Roberts, 1997) was proposed to explain one process through which sexist social experiences affect women’s mental health outcomes. Objectification theory posits that women are frequently treated as objects in Western society, and that they internalize this treatment such that they view themselves as objects. This selfobjectification affects their experience of themselves in the world, heightening body-related shame and appearance- and safety-related anxiety. It also makes it more difficult for women to feel connected with their bodies and to experience flow, a pleasant sensation of feeling absorbed in the present moment. Flow has a rich body of research dating back to at least 1975, when Csikszentmihalyi wrote about flow as experienced by chess players, dancers, rock climbers, and surgeons. Historically, however, objectification theory researchers have used measures of flow not grounded in Csikszentmihalyi’s multi-dimensional conceptualization. One purpose of the present study was to investigate the aspects of flow most relevant to objectification theory (i.e., concentration, control, and loss of selfconsciousness) using an appropriate, validated measure. A second purpose of the present study was to explore mindfulness and selfcompassion as potential moderators within the objectification theory framework. These strength-based practices have received recent attention for treatment of anxiety, depression, and other mental health concerns. We studied mindfulness and self-compassion at the trait level as a first step in exploring how these cultivatable strengths may buffer against the deleterious effects of objectification. The present study used a correlational design to explore relationships among objectification theory variables and hypothesized strength-based moderators. We sampled data obtained from 500 women recruited through three different methods who completed an online survey consisting of 11 different measures. Data were analyzed using structural equation modeling. Hypothesized moderated relationships were generally not supported, although most correlations were in the expected directions. Overall, results underscored the need to a) study flow within the objectification theory framework using a multi-dimensional conceptualization and b) develop strength-based interventions for treating women’s mental health concerns.Introduction -- Background and review of literature -- Research design and methodology -- Results -- Discussion -- Appendix A. Screening questions -- Appendix B. Demographic questionnaire -- Appendix C. The body surveillance subscale of the objectified body -- Appendix D. The body shame subscale of the objectified body consciousness scale -- Appendix E. The social appearance anxiety scale -- Appendix F. The dispositional flow scale-2 long form -- Appendix G. Measure of physical safety anxiety -- Appendix H. Measure of body responsiveness -- Appendix I. The eating attitudes test -- Appendix J. The center for epidemiologic studies depression scale-short form -- Appendix K. The female sexual function index -- Appendix L. The Freiburg mindfulness inventory-short form -- Appendix M. The self-compassion scale-short form -- Appendix N. AMOS proposed model of the mediating role of the three dimensions of flow in objectification theory -- Appendix O. AMOS retained model of the mediating role of the three dimensions of flow in objectiticaiton theory -- Appendix P. AMOS modified proposed model of the moderating role of dispositional mindefullness in objectification theory -- Appendix Q. AMOS retained model of the moderating role of dispositional mindefullness in objectification theory -- Appendix R. AMOS modified proposed model of the moderating role of self-compassion in objectification theory -- Appendix S. AMOS retained model of the moderating role of self-compassion in objectification theor

Contrasting influences of Drosophila white/mini-white on ethanol sensitivity in two different behavioral assays

Background The fruit fly Drosophila melanogaster has been used extensively to investigate genetic mechanisms of ethanol-related behaviors. Many past studies in flies, including studies from our laboratory, have manipulated gene expression using transposons carrying the genetic-phenotypic marker mini-white, a derivative of the endogenous gene white. Whether the mini-white transgenic marker or the endogenous white gene influence behavioral responses to acute ethanol exposure in flies has not been systematically investigated. Methods We manipulated mini-white and white expression via (i) transposons marked with mini-white, (ii) RNAi against mini-white and white and (iii) a null allele of white. We assessed ethanol sensitivity and tolerance using a previously described eRING assay (based on climbing in the presence of ethanol) and an assay based on ethanol-induced sedation. Results In eRING assays, ethanol-induced impairment of climbing correlated inversely with expression of the mini-white marker from a series of transposon insertions. Additionally, flies harboring a null allele of white or flies with RNAi-mediated knockdown of mini-white were significantly more sensitive to ethanol in eRING assays than controls expressing endogenous white or the mini-white marker. In contrast, ethanol sensitivity and rapid tolerance measured in the ethanol sedation assay were not affected by decreased expression of mini-white or endogenous white in flies. Conclusions Ethanol sensitivity measured in the eRING assay is noticeably influenced by white and mini-white, making eRING problematic for studies on ethanol-related behavior in Drosophila using transgenes marked with mini-white. In contrast, the ethanol sedation assay described here is a suitable behavioral paradigm for studies on ethanol sedation and rapid tolerance in Drosophila including those that use widely available transgenes marked with mini-white

Dietary yeast influences ethanol sedation in Drosophila via serotonergic neuron function

Abuse of alcohol is a major clinical problem with far- reaching health consequences. Understanding the environmental and genetic factors that contribute to alcohol- related behaviors is a potential gateway for developing novel therapeutic approaches for patients that abuse the drug. To this end, we have used Drosophila melanogaster as a model to investigate the effect of diet, an environmental factor, on ethanol sedation. Providing flies with diets high in yeast, a routinely used component of fly media, increased their resistance to ethanol sedation. The yeast- induced resistance to ethanol sedation occurred in several different genetic backgrounds, was observed in males and females, was elicited by yeast from different sources, was readily reversible, and was associated with increased nutrient intake as well as decreased internal ethanol levels. Inhibition of serotonergic neuron function using multiple independent genetic manipulations blocked the effect of yeast supplementation on ethanol sedation, nutrient intake, and internal ethanol levels. Our results demonstrate that yeast is a critical dietary component that influences ethanol sedation in flies and that serotonergic signaling is required for the effect of dietary yeast on nutrient intake, ethanol uptake/elimination, and ethanol sedation. Our studies establish the fly as a model for diet- induced changes in ethanol sedation and raise the possibility that serotonin might mediate the effect of diet on alcohol- related behavior in other species.Flies fed a high yeast diet consume more nutrients, have decreased levels of internal ethanol when exposed to ethanol vapor and require longer exposure to ethanol to become sedated (ie, increased ST50). Our studies implicate serotonergic neurons as key regulators of nutrient consumption and therefore, the effect of dietary yeast on ethanol sedation in flies.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/155987/1/adb12779.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/155987/2/adb12779_am.pd

Mushroom Body Ablation Impairs Short-Term Memory and Long-Term Memory of Courtship Conditioning in Drosophila melanogaster

AbstractWe have evaluated the role of the Drosophila mushroom bodies (MBs) in courtship conditioning, in which experience with mated females causes males to reduce their courtship toward virgins (Siegel and Hall 1979). Whereas previous studies indicated that MB ablation abolished learning in an olfactory conditioning paradigm (deBelle and Heisenberg 1994), MB-ablated males were able to learn in the courtship paradigm. They resumed courting at naive levels within 30 min after training, however, while the courtship of control males remained depressed 1 hr after training. We also describe a novel courtship conditioning paradigm that established long-term memory, lasting 9 days. In MB-ablated males, memory dissipated completely within 1 day. Our results indicate that the MBs are not required for learning and immediate recall of courtship conditioning but are required for consolidation of short-term and long-term associative memories

Senescence of the cellular immune response in Drosophila melanogaster

Immune system effectiveness generally declines as animals age, compromising disease resistance. In Drosophila, expression of a variety of immune-related genes elevates during ageing; however how this is linked to increasing pathogen susceptibility in older flies has remained unclear. We investigated whether changes in the Drosophila cellular immune response might contribute to immunosenescence. Experiments studied fly cohorts of different ages and compared the numbers and activity of the circulating haemocytes involved in pathogen defence. In female wildtype Samarkand and Oregon R flies the haemocyte population fell by 31.8% and 10.2% respectively during the first four weeks of adulthood. Interestingly we detected no such decline in male flies. The impact of ageing on the phagocytic activity of haemocytes was investigated by injecting flies with fluorescently labelled microbes or latex beads and assessing the ability of haemocytes to engulf them. For all immune challenges the proportion of actively phagocytosing haemocytes decreased as flies aged. Whilst 24.3% ± 1.15% of haemocytes in one-week-old flies phagocytosed Escherichia coli bacteria or Beauveria bassiana fungal spores, this decreased to 16.7% ± 0.99% in four-week-old flies. This clear senescence of the Drosophila cellular immune response may underpin increased disease susceptibility in older flies

In the Laboratory and during Free-Flight: Old Honey Bees Reveal Learning and Extinction Deficits that Mirror Mammalian Functional Decline

Loss of brain function is one of the most negative and feared aspects of aging. Studies of invertebrates have taught us much about the physiology of aging and how this progression may be slowed. Yet, how aging affects complex brain functions, e.g., the ability to acquire new memory when previous experience is no longer valid, is an almost exclusive question of studies in humans and mammalian models. In these systems, age related cognitive disorders are assessed through composite paradigms that test different performance tasks in the same individual. Such studies could demonstrate that afflicted individuals show the loss of several and often-diverse memory faculties, and that performance usually varies more between aged individuals, as compared to conspecifics from younger groups. No comparable composite surveying approaches are established yet for invertebrate models in aging research. Here we test whether an insect can share patterns of decline similar to those that are commonly observed during mammalian brain aging. Using honey bees, we combine restrained learning with free-flight assays. We demonstrate that reduced olfactory learning performance correlates with a reduced ability to extinguish the spatial memory of an abandoned nest location (spatial memory extinction). Adding to this, we show that learning performance is more variable in old honey bees. Taken together, our findings point to generic features of brain aging and provide the prerequisites to model individual aspects of learning dysfunction with insect models

Neuregulin-1 Regulates Cell Adhesion via an ErbB2/Phosphoinositide-3 Kinase/Akt-Dependent Pathway: Potential Implications for Schizophrenia and Cancer

Neuregulin-1 (NRG1) is a putative schizophrenia susceptibility gene involved extensively in central nervous system development as well as cancer invasion and metastasis. Using a B lymphoblast cell model, we previously demonstrated impairment in NRG1alpha-mediated migration in cells derived from patients with schizophrenia as well as effects of risk alleles in NRG1 and catechol-O-methyltransferase (COMT), a second gene implicated both in schizophrenia susceptibility and in cancer.Here, we examine cell adhesion, an essential component process of cell motility, using an integrin-mediated cell adhesion assay based on an interaction between ICAM-1 and the CD11a/CD18 integrin heterodimer expressed on lymphoblasts. In our assay, NRG1alpha induces lymphoblasts to assume varying levels of adhesion characterized by time-dependent fluctuations in the firmness of attachment. The maximum range of variation in adhesion over sixty minutes correlates strongly with NRG1alpha-induced migration (r(2) = 0.61). NRG1alpha-induced adhesion variation is blocked by erbB2, PI3K, and Akt inhibitors, but not by PLC, ROCK, MLCK, or MEK inhibitors, implicating the erbB2/PI3K/Akt1 signaling pathway in NRG1-stimulated, integrin-mediated cell adhesion. In cell lines from 20 patients with schizophrenia and 20 normal controls, cells from patients show a significant deficiency in the range of NRG1alpha-induced adhesion (p = 0.0002). In contrast, the response of patient-derived cells to phorbol myristate acetate is unimpaired. The COMT Val108/158Met genotype demonstrates a strong trend towards predicting the range of the NRG1alpha-induced adhesion response with risk homozygotes having decreased variation in cell adhesion even in normal subjects (p = 0.063).Our findings suggest that a mechanism of the NRG1 genetic association with schizophrenia may involve the molecular biology of cell adhesion

Characterization of lamin Mutation Phenotypes in Drosophila and Comparison to Human Laminopathies

Lamins are intermediate filament proteins that make up the nuclear lamina, a matrix underlying the nuclear membrane in all metazoan cells that is important for nuclear form and function. Vertebrate A-type lamins are expressed in differentiating cells, while B-type lamins are expressed ubiquitously. Drosophila has two lamin genes that are expressed in A- and B-type patterns, and it is assumed that similarly expressed lamins perform similar functions. However, Drosophila and vertebrate lamins are not orthologous, and their expression patterns evolved independently. It is therefore of interest to examine the effects of mutations in lamin genes. Mutations in the mammalian lamin A/C gene cause a range of diseases, collectively called laminopathies, that include muscular dystrophies and premature aging disorders. We compared the sequences of lamin genes from different species, and we have characterized larval and adult phenotypes in Drosophila bearing mutations in the lam gene that is expressed in the B-type pattern. Larvae move less and show subtle muscle defects, and surviving lam adults are flightless and walk like aged wild-type flies, suggesting that lam phenotypes might result from neuromuscular defects, premature aging, or both. The resemblance of Drosophila lam phenotypes to human laminopathies suggests that some lamin functions may be performed by differently expressed genes in flies and mammals. Such still-unknown functions thus would not be dependent on lamin gene expression pattern, suggesting the presence of other lamin functions that are expression dependent. Our results illustrate a complex interplay between lamin gene expression and function through evolution