Prirodni i proizvedeni radionuklidi u uzorcima mahovina iz regiona Severne Grčke

Terrestrial mosses obtain most of their nutrients directly from precipitation and dry deposition, and they can be used for monitoring of airborne radionuclide depositions. Ninetyfive samples of Hypnum Cupressiforme were collected in Northern Greece during the end of summer 2016. After the preparation, mosses were measured in a low-background HPGe detector with relative efficiency 32%. The activity concentrations of 7 Be ranged from 69 to 1280 Bq kg-1, and the concentrations of 137Cs ranged from 0 to 425 Bq kg-1. The concentrations of 210Pb were between 147 and 1920 Bq kg-1 and for 40K were between 120 and 750 Bq kg-1. Differences have been observed in the activity concentrations between the mosses collected from ground surface, rocks, branches and near roots. Finally, no correlation between the concentrations of 7 Be and 210Pb has been found.Mahovine svoje nutrijente direktno dobijaju iz padavina i suvom depozicijom i mogu se koristiti za monitoring depozicije radionuklida iz vazduha. Devedesetpet uzoraka mahovina (Hypnum Cupressiforme) je sakupljeno u regionu Severne Grčke tokom kraja leta 2016. godine. Posle pripreme, mahovine su merene na niskofonskom HPGe detektoru relativne efikasnosti 32%. Izmerene koncentracije aktivnosti 7 Be su bile od 69 do 1280 Bq kg-1, aktivnosti 137Cs od 0 do 425 Bq kg-1, aktivnosti 210Pb od 147 do 1920 Bq kg-1 , a 40K od 120 do 750 Bq kg-1. Primećene su razlike u koncentracijama aktivnosti radionuklida u mahovinama koje su sakupljene sa zemlje, kamena, grana stabala i blizu korena stabla. Između 7 Be i 210Pb nije pronađena nikakva korelacija

Prirodni i proizvedeni radionuklidi u uzorcima mahovina iz regiona Severne Grčke

Terrestrial mosses obtain most of their nutrients directly from precipitation and dry deposition, and they can be used for monitoring of airborne radionuclide depositions. Ninetyfive samples of Hypnum Cupressiforme were collected in Northern Greece during the end of summer 2016. After the preparation, mosses were measured in a low-background HPGe detector with relative efficiency 32%. The activity concentrations of 7 Be ranged from 69 to 1280 Bq kg-1, and the concentrations of 137Cs ranged from 0 to 425 Bq kg-1. The concentrations of 210Pb were between 147 and 1920 Bq kg-1 and for 40K were between 120 and 750 Bq kg-1. Differences have been observed in the activity concentrations between the mosses collected from ground surface, rocks, branches and near roots. Finally, no correlation between the concentrations of 7 Be and 210Pb has been found.Mahovine svoje nutrijente direktno dobijaju iz padavina i suvom depozicijom i mogu se koristiti za monitoring depozicije radionuklida iz vazduha. Devedesetpet uzoraka mahovina (Hypnum Cupressiforme) je sakupljeno u regionu Severne Grčke tokom kraja leta 2016. godine. Posle pripreme, mahovine su merene na niskofonskom HPGe detektoru relativne efikasnosti 32%. Izmerene koncentracije aktivnosti 7 Be su bile od 69 do 1280 Bq kg-1, aktivnosti 137Cs od 0 do 425 Bq kg-1, aktivnosti 210Pb od 147 do 1920 Bq kg-1 , a 40K od 120 do 750 Bq kg-1. Primećene su razlike u koncentracijama aktivnosti radionuklida u mahovinama koje su sakupljene sa zemlje, kamena, grana stabala i blizu korena stabla. Između 7 Be i 210Pb nije pronađena nikakva korelacija

Towards norms for accreditation of biobanks for human health and medical research:Compilation of existing guidelines into an ISO certification/accreditation norm-compatible format

In recent years, biobanks have evolved into professional infrastructures that acquire, validate, process, store, manage and distribute biological material of human origin to public or private end-users/researchers. This article (a) highlights the importance of quality assurance for both the biobank basic processes and sample annotation in order to ensure reliable results of research based on these samples, (b) suggests that certification according to international standards can contribute to the organization of the biobanking processes while accreditation can contribute to the organization of sample characterization/validation, and (c) provides a compilation of all existing guidelines against an International Organization for Standardization (ISO) format.</p

Prostorna distribucija prirodnih radionuklida merena u Srbiji upotrebom biomonitora

Активност природних радионуклида је мерена у 217 узорака маховина Hypnum cupressiforme које су сакупљене крајем лета 2015. године на комплетној територији Републике Србије са циљем да се установи просторна дистрибуција радионуклида. Мерења су вршена NaI детектором облика јаме. Посебна пажња је посвећена 7Be који се већ деценијама користи као природни обележивач у изучавању транспортних процеса у атмосфери. Добијено је да је дистрибуција атмосферске депозиције 7Be неуниформна и да се најмања и највећа измерена вредност разликују девет пута. Просторна дистрибуција 7Be не показује корелацију са рељефом терена за разлику од 137Cs кога има више у планинским и шумовитим пределима. Присутност радионуклида из урановог и торијумовог низа у доброј мери зависи од структуре и састава тла на локацијама са којих су узимани узорци.The activities of natural radionuclide were measured in 217 moss samples that were collected at the entire territory of Serbia. Measurements were taken by well-type NaI detector in order to establish the spatial distribution of radionuclides. Special attention was paid to 7Be. It is obtained that the distribution of atmospheric deposition of 7Be is non-uniform; the minimum and maximum measured value differs nine times. No coincidence of the spatial distribution of 7Be with the relief was observed. It was noticed that higher values of 137Cs were detected in mountain and wooded areas. The presence of radionuclides from the Uranium and Thorium chains in the large extent depends on the structure of the soil at the sampling site

Recommandations préanalytiques pour les analyses de protéomique clinique des fluides biologiques

International audienc

LacaScore: a novel plasma sample quality control tool based on ascorbic acid and lactic acid levels

Introduction Metabolome analysis is complicated by the continuous dynamic changes of metabolites in vivo and ex vivo. One of the main challenges in metabolomics is the robustness and reproducibility of results, partially driven by pre-analytical variations. Objectives The objective of this study was to analyse the impact of pre-centrifugation time and temperature, and to determine a quality control marker in plasma samples. Methods Plasma metabolites were measured by gas chromatography-mass spectrometry (GC–MS) and analysed with the MetaboliteDetector software. The metabolites, which were the most labile to pre-analytical variations, were further measured by enzymatic assays. A score was calculated for their use as quality control markers. Results The pre-centrifugation temperature was shown to be critical in the stability of plasma samples and had a significant impact on metabolite concentration profiles. In contrast, pre-centrifugation delay had only a minor impact. Based on the results of this study, whole blood should be kept on wet ice and centrifuged within maximum 3 h as a prerequisite for preparing EDTA plasma samples fit for the purpose of metabolome analysis. Conclusions We have established a novel blood sample quality control marker, the LacaScore, based on the ascorbic acid to lactic acid ratio in plasma, which can be used as an indicator of the blood pre-centrifugation conditions, and hence the suitability of the sample for metabolome analyses. This method can be applied in research institutes and biobanks, enabling assessment of the quality of their plasma sample collections

Tuberculosis diagnostics and biomarkers: needs, challenges, recent advances, and opportunities

Tuberculosis is unique among the major infectious diseases in that it lacks accurate rapid point-of-care diagnostic tests. Failure to control the spread of tuberculosis is largely due to our inability to detect and treat all infectious cases of pulmonary tuberculosis in a timely fashion, allowing continued Mycobacterium tuberculosis transmission within communities. Currently recommended gold-standard diagnostic tests for tuberculosis are laboratory based, and multiple investigations may be necessary over a period of weeks or months before a diagnosis is made. Several new diagnostic tests have recently become available for detecting active tuberculosis disease, screening for latent M. tuberculosis infection, and identifying drug-resistant strains of M. tuberculosis. However, progress toward a robust point-of-care test has been limited, and novel biomarker discovery remains challenging. In the absence of effective prevention strategies, high rates of early case detection and subsequent cure are required for global tuberculosis control. Early case detection is dependent on test accuracy, accessibility, cost, and complexity, but also depends on the political will and funder investment to deliver optimal, sustainable care to those worst affected by the tuberculosis and human immunodeficiency virus epidemics. This review highlights unanswered questions, challenges, recent advances, unresolved operational and technical issues, needs, and opportunities related to tuberculosis diagnostics

A Year in the Life of the EU-CardioRNA COST Action: CA17129 Catalysing Transcriptomics Research in Cardiovascular Disease

The EU-CardioRNA Cooperation in Science and Technology (COST) Action is a European-wide consortium established in 2018 with 31 European country members and four associate member countries to build bridges between translational researchers from academia and industry who conduct research on non-coding RNAs, cardiovascular diseases and similar research areas. EU-CardioRNA comprises four core working groups (WG1-4). In the first year since its launch, EU-CardioRNA met biannually to exchange and discuss recent findings in related fields of scientific research, with scientific sessions broadly divided up according to WG. These meetings are also an opportunity to establish interdisciplinary discussion groups, brainstorm ideas and make plans to apply for joint research grants and conduct other scientific activities, including knowledge transfer. Following its launch in Brussels in 2018, three WG meetings have taken place. The first of these in Lisbon, Portugal, the second in Istanbul, Turkey, and the most recent in Maastricht, The Netherlands. Each meeting includes a scientific session from each WG. This meeting report briefly describes the highlights and key take-home messages from each WG session in this first successful year of the EU-CardioRNA COST Action. © 2020 by the authors

PENGARUH TEKANAN KETAATAN DAN KOMPLEKSITAS TUGAS TERHADAP AUDIT JUDGMENT (Survey Terhadap Lima Kantor AkuntanPublik di Kota Bandung)

ABSTRAK Seperti yang kita ketahui bahwa seorang auditor dalam melakukan tugasnya membuat audit judgment dipengaruhi banyak faktor, baik bersifat teknis dan non teknis. Salah satu faktor non teknis adalah aspek perilaku individual. Aspek perilaku individu, sebagai salah satu faktor yang banyak mempengaruhi pembuatan audit judgment. Pada penelitian ini ada beberapa faktor yang mempengaruhi audit judgment yaitu tekanan ketaatan dan kompleksitas tugas. Dalam penelitian ini penullis ingin mengetahui sejauh mana “tekanan ketaatan dan kompleksitas tugas terhadap audit judgment”. Sedangkan tujuan dari penelitian ini adalah untuk mengetahui dan mempelajari tekanan ketaatan dan kompleksitas tugas terhadap audit judgment. Hipotesis yang diuji dalam penelitian ini adalah “ jika tekanan ketaatan dan kompleksitas tugas baik, maka audit judgment akan meningkat ( baik pula)”. Hipotesis ini berdasarkan asumsi bahwa tekanan ketaatan dan kompleksitas tugas berpengaruh terhadap audit judgment.dalam penelitian ini penulis menggunakan metode deskriptif asosiatif dengan pendekatan survey dan tes statistik. Penelitian ini terdiri dari atas variabel X1 dan X2 dan audit judgment sebagai veriabel Y atau variabel independen. Uji statistik dilakukan dengan mengolah data dari hasil jawaban kuesioner. Dalam penelitian ini, peulis menyebarkan angket kepada 5 Kantor Akuntan Publik di Kota Bandung khusunya untuk para auditor. Pengumpulan data dilakukan dengan cara penyebaran kuesioner yang telah diuji validitasnya dan reabilitasnya. Penelitian ini dilakukan di 5 KAP di Kota Bandung. Pengambilan sampel ini menggunakan purposive sampling berukuran 28 orang responden. Untuk uji hipotesis penelitian, penulis melakukannya dengan uji t untuk masing-masing variabel X1,X2, dan Y. Dari hasil uji tHitung tekanan ketaatan terhadap audit judgment tHitung =4,178>ttabel = 1.705 kompleksitas tugas terhadap audit judgment 5 tHitung = 3.364 > ttabel = 1,705. Maka, dari hasil uji hipotesis tersebut penulis menyimpulkan bahwa hipotesis penelitian diterima (Ho ditolak, Ha diterima) artinya terdapat pengaruh antara terkanan ketaatan terhadap audit judgment dan kompleksitas tugas terhadap audit judgment Untuk mencari besarnya pengaruh Tekanan ketaatan dan Kompleksitas Tugas terhadap Audit Judgment secara simultan penulis melakukannya dengan uji f dengan koefisien determinasi (KD). Dari hasil uji fhitung dan > f table yaitu 16,182>3,370. Kata kunci : Tekanan Ketaatan dan Kompleksitas tugas Terhadap Audit Judgmen