121 research outputs found
Shape and phase control of CdS nanocrystals using cationic surfactant in noninjection synthesis
Monodispersed CdS nanocrystals with controllable shape and phase have been successfully synthesized in this study by adding cationic surfactant in noninjection synthesis system. With the increase of the amount of cetyltrimethylammonium chloride (CTAC) added, the shape of the CdS nanocrystals changed from spherical to multi-armed, and the phase changed from zinc-blende to wurtzite. It was found that halide ion Cl- plays a key role in the transformation, and other halide ions such as Br- can also induce similar transformation. We proposed that the strong binding between Cd2+ and halide ions reduced the reactivity of the precursors, decreased the nuclei formed in the nucleation stage, and led to the high concentration of precursor in the growth stage, resulting in the increase of size and phase transformation of CdS nanocrystals. In addition, it was found that the multi-armed CdS nanocrystals lost quantum confinement effect because of the increase of the size with the increase of the concentration of CTAC
The effect of dopant and optical micro-cavity on the photoluminescence of Mn-doped ZnSe nanobelts
Pure and Mn-doped ZnSe nanobelts were synthesized by a convenient thermal evaporation method. Scanning electron microscopy, X-ray powder diffraction, energy dispersive X-ray spectroscopy and corresponding element mapping, and transmission electron microscope were used to examine the morphology, phase structure, crystallinity, composition, and growth direction of as-prepared nanobelts. Raman spectra were used to confirm the effective doping of Mn(2+) into ZnSe nanobelts. Micro-photoluminescence (PL) spectra were used to investigate the emission property of as-prepared samples. A dominant trapped-state emission band is observed in single ZnSe(Mn) nanobelt. However, we cannot observe the transition emission of Mn ion in this ZnSe(Mn) nanobelt, which confirm that Mn powder act as poor dopant. There are weak near-bandgap emission and strong (4)T(1) â (6)A(1) transition emission of Mn(2+) in single [Formula: see text] and [Formula: see text] nanobelt. More interesting, the (4)T(1) â (6)A(1) transition emission in [Formula: see text] nanobelt split into multi-bands. PL mapping of individual splitted sub-bands were carried out to explore the origin of multi-bands. These doped nanobelts with novel multi-bands emission can find application in frequency convertor and wavelength-tunable light emission devices
Coexistence of Aedes aegypti and Aedes albopictus in Jinghong City, Yunnan Province: A Survey of Aedes aegypti Invasion
We conducted this study to confirm that Aedes aegypti has invaded and colonized in Xishuangbanna, Yunan province, China. And we conducted a larval survey for dengue mosquito vectors, Ae. aegypti and Ae. albopictus, to assess on possible competition for breeding sites between these both species in urban environment in Jinghong city. The study was carried out at least once a month in February and March, November and December, and three times for each month from April to October in 2014 in five communities in the municipality of Jinghong city. No less than 50 houses were surveyed each time in one place. We collected immature stages of mosquitoes from all types of breeding sites in houses and their premises. Morphological species identification was carried for Ae. aegypti and Ae. albopictus. Both species occurred in all surveyed areas. Aedes aegypti has occupied a considerable composition (67.75%) since the uncertain time of its first invasion to Jinghong city. Aedes aegypti was predominant species indoor (74.19%), but also presented in surrounding environment (25.81%); while Ae. albopictus favored the outdoor environment (54.55%) as well as in houses (45.45%). We gave the preference water containers (tires) for dengue vectors, described different distribution in different investigated places, and showed with monthly variance by indices of BI and CI. We gave our rational explanation for the variance with raining in this area, Aedes keep a high density value during the rainy season. For both dengue vectors, the possibility cannot be excluded that the invasion of Ae. aegypti in the city might lead to a decrease or elimination of Ae. albopictus in houses and dwellings. Furthermore, we also found that both dengue vectors have already occupied the breeding sites in houses and their premises with water container in Jinghong city, and both should be managed during dengue epidemic season. These findings from this study are relevant for dengue vector control programs
Exosomes from embryonic mesenchymal stem cells alleviate osteoarthritis through balancing synthesis and degradation of cartilage extracellular matrix
Abstract
Background
Mesenchymal stem cell therapy for osteoarthritis (OA) has been widely investigated, but the mechanisms are still unclear. Exosomes that serve as carriers of genetic information have been implicated in many diseases and are known to participate in many physiological processes. Here, we investigate the therapeutic potential of exosomes from human embryonic stem cell-induced mesenchymal stem cells (ESC-MSCs) in alleviating osteoarthritis (OA).
Methods
Exosomes were harvested from conditioned culture media of ESC-MSCs by a sequential centrifugation process. Primary mouse chondrocytes treated with interleukin 1 beta (IL-1β) were used as an in vitro model to evaluate the effects of the conditioned medium with or without exosomes and titrated doses of isolated exosomes for 48 hours, prior to immunocytochemistry or western blot analysis. Destabilization of the medial meniscus (DMM) surgery was performed on the knee joints of C57BL/6 J mice as an OA model. This was followed by intra-articular injection of either ESC-MSCs or their exosomes. Cartilage destruction and matrix degradation were evaluated with histological staining and OARSI scores at the post-surgery 8 weeks.
Results
We found that intra-articular injection of ESC-MSCs alleviated cartilage destruction and matrix degradation in the DMM model. Further in vitro studies illustrated that this effect was exerted through ESC-MSC-derived exosomes. These exosomes maintained the chondrocyte phenotype by increasing collagen type II synthesis and decreasing ADAMTS5 expression in the presence of IL-1β. Immunocytochemistry revealed colocalization of the exosomes and collagen type II-positive chondrocytes. Subsequent intra-articular injection of exosomes derived from ESC-MSCs successfully impeded cartilage destruction in the DMM model.
Conclusions
The exosomes from ESC-MSCs exert a beneficial therapeutic effect on OA by balancing the synthesis and degradation of chondrocyte extracellular matrix (ECM), which in turn provides a new target for OA drug and drug-delivery system development
Chemistry of new particle formation and growth events during wintertime in suburban area of Beijing : Insights from highly polluted atmosphere
The high frequency of new particle formation (NPF) events observed under polluted atmospheric conditions is still poorly understood. To improve our understanding of NPF and its effects, the particle number size distribution (3-1000 nm) and submicron particle chemical composition were measured from 4 November 2017 to 17 January 2018 in suburban Beijing. During this intense campaign, 22 NPF events were identified with a frequency of 29%, including 11 cases that occurred under "clean" conditions (C-NPF) and 11 cases that occurred under "polluted" conditions (P-NPF). The observed formation rate (J(3)) and condensation sink were 4.6-148.9 cm(-3).s(-1) and 0.01-0.07 s(-1), and the majority of NPF events occurred when the condensation sink (CS) values below 0.03 s(-1), indicating that condensation vapor likely constitutes the critical limiting factor for NPF events. The correlations between log J(3) and [H2SO4] that close to previous CLOUD experimental results in the majority of NPF events (68%) suggest the high nucleation rates (up to 100 cm(-3).s(-1)) would be attributed by the amines that enhancing sulfuric acid nucleation, while the reminding cases (32%) possibly attributed to the H2SO4-NH3 clustering mechanism, which is supported by the theoretical expectations for H2SO4 nucleation with NH3 simulated by the MALTE_BOX model. The observed growth rate varied from 4.9 to 37.0 mm.h(-1), with the dominant contribution (>60%) from sulfuric acid during the early phases of growth (similar to 4 nm), which was also sufficient to explain the observed Q(GR) for 50 nm)Peer reviewe
The plasticity of mesenchymal stem cells in regulating surface HLA-I
Summary A low surface expression level of human leukocyte antigen class I(HLA-I) ensures the mesenchymal stem cellsâ(MSCs) escape from the allogeneic recipientsâ immunological surveillance. Here, we discovered that both transcriptional and synthesis levels of HLA-I in MSCs increased continuously after IFN-Îł treatment, while interestingly, their surface HLA-I expression was downregulated after reaching an HLA-I surface expression peak. Microarray data indicated the post-transcriptional process plays an important role in downregulation of surface HLA-I. Further studies identified that IFN-Îł-treated MSCs accelerated HLA-I endocytosis through a Clathrinâindependent Dynamin-dependent endocytosis pathway. Furthermore, the cells which have self-downregulated surface HLA-I expression elicit a weaker immune response than they previously could. Thus, uncovering the plasticity of MSCs in the regulation of HLA-I surface expression would reveal insights into the membrane-transportation events leading to the maintenance of low surface HLA-I expression, providing more evidence for selecting and optimizing low immunogenic MSCs to improve the therapeutic efficiency.Peer reviewe
Noninjection Synthesis of CdS and Alloyed CdSxSe1âxNanocrystals Without Nucleation Initiators
CdS and alloyed CdSxSe1âx nanocrystals were prepared by a simple noninjection method without nucleation initiators. Oleic acid (OA) was used to stabilize the growth of the CdS nanocrystals. The size of the CdS nanocrystals can be tuned by changing the OA/Cd molar ratios. On the basis of the successful synthesis of CdS nanocrystals, alloyed CdSxSe1âx nanocrystals can also be prepared by simply replacing certain amount of S precursor with equal amount of Se precursor, verified by TEM, XRD, EDX as well as UVâVis absorption analysis. The optical properties of the alloyed CdSxSe1âx nanocrystals can be tuned by adjusting the S/Se feed molar ratios. This synthetic approach developed is highly reproducible and can be readily scaled up for potential industrial production
Experimental study on pool boiling in a porous artery structure
In this work, a porous artery structure is proposed to enhance the critical heat flux (CHF) of pool boiling based on the concept of âphase separation and modulationâ and extensive experimental studies have been carried out for validation. In the experiment, multiple rectangular arteries were machined directly into the top surface of a copper rod to provide individual flow paths for vapor escaping. The arteries were covered by a microporous copper plate where capillary forces can be developed at the liquid/vapor interface to prevent the vapor from penetrating the porous structure and realize strong liquid suction simultaneously. The pool wall was made of transparent quartz glass to enable a visualization study where the liquid/vapor distribution and movement can be observed directly. Favorable results have been reached as expected, and a maximum heat flux up to 805 W/cm2 was achieved with no indication of any dry-out, which successfully validated this new concept. In addition, the effects of the diameter and thickness of the porous copper plate, and the connection method between the porous copper plate and copper fin on the pool boiling heat transfer in the porous artery structure were investigated, and the inherent physical mechanisms were analyzed and discussed
Characterization and source identification of fine particulate matter in urban Beijing during the 2015 Spring Festival
The Spring Festival (SF) is the most important holiday in China for family reunion and tourism. During the 2015 SF an intensive observation campaign of air quality was conducted to study the impact of the anthropogenic activities and the dynamic characteristics of the sources. During the study period, pollution episodes frequently occurred with 12 days exceeding the Chinese Ambient Air Quality Standards for 24-h average PM2.5 (75 Îźg/m3), even 8 days with exceeding 150 Îźg/m3. The daily maximum PM2.5 concentration reached 350 Îźg/m3 while the hourly minimum visibility was <0.8 km. Three pollution episodes were selected for detailed analysis including chemical characterization and diurnal variation of the PM2.5 and its chemical composition, and sources were identified using the Positive Matrix Factorization model. The first episode occurring before the SF was characterized by more formation of SO42â and NO3â and high crustal enrichment factors for Ag, As, Cd, Cu, Hg, Pb, Se and Zn and seven categories of pollution sources were identified, whereby vehicle emission contributed 38% to the PM2.5. The second episode occurring during the SF was affected heavily by large-scale firework emissions, which led to a significant increase in SO42â, Clâ, OC, K and Ba; these emissions were the largest contributor to the PM2.5 accounting for 36%. During the third episode occurring after the SF, SO42â, NO3â, NH4+ and OC were the major constituents of the PM2.5 and the secondary source was the dominant source with a contribution of 46%. The results provide a detailed understanding on the variation in occurrence, chemical composition and sources of the PM2.5 as well as of the gaseous pollutants affected by the change in anthropogenic activities in Beijing throughout the SF. They highlight the need for limiting the firework emissions during China's most important traditional festival
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