Quantitation of the distribution and flux of myosin-II during cytokinesis

BACKGROUND: During cytokinesis, the cell's equator contracts against the cell's global stiffness. Identifying the biochemical basis for these mechanical parameters is essential for understanding how cells divide. To achieve this goal, the distribution and flux of the cell division machinery must be quantified. Here we report the first quantitative analysis of the distribution and flux of myosin-II, an essential element of the contractile ring. RESULTS: The fluxes of myosin-II in the furrow cortex, the polar cortex, and the cytoplasm were examined using ratio imaging of GFP fusion proteins expressed in Dictyostelium. The peak concentration of GFP-myosin-II in the furrow cortex is 1.8-fold higher than in the polar cortex and 2.0-fold higher than in the cytoplasm. The myosin-II in the furrow cortex, however, represents only 10% of the total cellular myosin-II. An estimate of the minimal amount of this motor needed to produce the required force for cell cleavage fits well with this 10% value. The cell may, therefore, regulate the amount of myosin-II sent to the furrow cortex in accordance with the amount needed there. Quantitation of the distribution and flux of a mutant myosin-II that is defective in phosphorylation-dependent thick filament disassembly confirms that heavy chain phosphorylation regulates normal recruitment to the furrow cortex. CONCLUSION: The analysis indicates that myosin-II flux through the cleavage furrow cortex is regulated by thick filament phosphorylation. Further, the amount of myosin-II observed in the furrow cortex is in close agreement with the amount predicted to be required from a simple theoretical analysis

The mass-metallicity gradient relation of early-type galaxies

We present a newly observed relation between galaxy mass and radial metallicity gradients of early-type galaxies. Our sample of 51 early-type galaxies encompasses a comprehensive mass range from dwarf to brightest cluster galaxies. The metallicity gradients are measured out to one effective radius by comparing nearly all of the Lick absorption-line indices to recent models of single stellar populations. The relation shows very different behaviour at low and high masses, with a sharp transition being seen at a mass of ~ 3.5 x 10^10 M_sun (velocity dispersion of ~140 km/s, M_B ~ -19). Low-mass galaxies form a tight relation with mass, such that metallicity gradients become shallower with decreasing mass and positive at the very low-mass end. Above the mass transition point several massive galaxies have steeper gradients, but a clear downturn is visible marked by a broad scatter. The results are interpreted in comparison with competing model predictions. We find that an early star-forming collapse could have acted as the main mechanism for the formation of low-mass galaxies, with star formation efficiency increasing with galactic mass. The high-mass downturn could be a consequence of merging and the observed larger scatter a natural result of different merger properties. These results suggest that galaxies above the mass threshold of ~ 3.5 x 10^10 M_sun might have formed initially by mergers of gas-rich disc galaxies and then subsequently evolved via dry merger events. The varying efficiency of the dissipative merger-induced starburst and feedback processes have shaped the radial metallicity gradients in these high-mass systems.Comment: 5 pageg, 3 figures, accepted by ApJ Lette

Spectroscopic evolution of dusty starburst galaxies

By using a one-zone chemical and spectrophotometric evolution model of a disk galaxy undergoing a dusty starburst, we investigate, numerically, the optical spectroscopic properties in order to explore galaxy evolution in distant clusters. We adopt an assumption that the degree of dust extinction (represented by $A_V$) depends on the ages of starburst populations in such a way that younger stars have larger $A_V$ (originally referred to as selective dust extinction by Poggianti & Wu 2000). In particular, we investigate how the time evolution of the equivalent widths of [OII]$\lambda$3727 and H$\delta$ is controlled by the adopted age dependence. This leads to three main results: (1) If a young stellar population (with the age of $\sim$ $10^6$ yr) is more heavily obscured by dust than an old one ($>$ $10^8$ yr), the galaxy can show an ``e(a)'' spectrum characterized by strong H$\delta$ absorption and relatively modest [OII] emission. (2) A dusty starburst galaxy with an e(a) spectrum can evolve into a poststarburst galaxy with an a+k (or k+a) spectrum 0.2 Gyr after the starburst and then into a passive one with a k-type spectrum 1 Gyr after the starburst. This result clearly demonstrates an evolutionary link between galaxies with different spectral classes (i.e., e(b), e(a), a+k, k+a, and k). (3) A dusty starburst galaxy can show an a+k or k+a spectrum even in the dusty starburst phase if the age-dependence of dust extinction is rather weak, i.e., if young starburst populations with different ages ($\le$ $10^7$ yr) are uniformly obscured by dust.Comment: 27 pages 12 figures,2001,ApJ,in pres

A study of central galaxy rotation with stellar mass and environment

© 2017. The American Astronomical Society. All rights reserved. We present a pilot analysis of the influence of galaxy stellar mass and cluster environment on the probability of slow rotation in 22 central galaxies at mean redshift z = 0.07. This includes new integral-field observations of five central galaxies selected from the Sloan Digital Sky Survey, observed with the SPIRAL integral-field spectrograph on the Anglo-Australian Telescope. The composite sample presented here spans a wide range of stellar masses, 10.9 < log(M∗/M⊙)lt; 12.0, and are embedded in halos ranging from groups to clusters, 12.9 < log(M 200 Ṁ) < 15.6. We find a mean probability of slow rotation in our sample of P(SR) = 54 ± 7%. Our results show an increasing probability of slow rotation in central galaxies with increasing stellar mass. However, when we examine the dependence of slow rotation on host cluster halo mass, we do not see a significant relationship. We also explore the influence of cluster dominance on slow rotation in central galaxies. Clusters with low dominance are associated with dynamically younger systems. We find that cluster dominance has no significant effect on the probability of slow rotation in central galaxies. These results conflict with a paradigm in which halo mass alone predetermines central galaxy properties

Quasi-stars, giants and the Sch\"onberg-Chandrasekhar limit

The Sch\"onberg-Chandrasekhar (SC) limit is a well-established result in the understanding of stellar evolution. It provides an estimate of the point at which an evolved isothermal core embedded in an extended envelope begins to contract. We investigate contours of constant fractional mass in terms of homology invariant variables U and V and find that the SC limit exists because the isothermal core solution does not intersect all the contours for an envelope with polytropic index 3. We find that this analysis also applies to similar limits in the literature including the inner mass limit for polytropic models of quasi-stars. Consequently, any core solution that does not intersect all the fractional mass contours exhibits an associated limit and we identify several relevant cases where this is so. We show that a composite polytrope is at a fractional core mass limit when its core solution touches but does not cross the contour of the corresponding fractional core mass. We apply this test to realistic models of helium stars and find that stars typically expand when their cores are near a mass limit. Furthermore, it appears that stars that evolve into giants have always first exceeded an SC-like limit.Comment: 9 pages, 7 figures, published in MNRAS. Updated to (more closely) match published versio

The structure and evolution of quasi-stars

The existence of bright quasars at high redshifts implies that supermassive black holes were able to form in the early Universe. Though a number of mechanisms to achieve this have been proposed, none yet stands out. A recent suggestion is the formation of quasi-stars, initially stellar-mass black holes accreting from hydrostatic giant-like envelopes of gas, formed from the monolithic collapse of pre-galactic gas clouds. In this work, we modify the Cambridge STARS stellar evolution package to construct detailed models of the evolution of these objects. We find that, in all of our models, the black hole inside the envelope is able to reach slightly more than one-tenth of the total mass of the system before hydrostatic equilibrium breaks down. This breakdown occurs after a few million years of evolution. We show that the mechanism which causes the hydrostatic evolution to end is present in polytropic models. We also show that the solutions are highly sensitive to the size of the inner boundary radius and that no physical solutions exist if the inner boundary is chosen to be less than about 0.3 of the Bondi radius.Comment: 12 pages, 11 figures. Published in MNRAS. Very belatedly updated to (more closely) match published versio

The Evolution of Bat Vestibular Systems in the Face of Potential Antagonistic Selection Pressures for Flight and Echolocation

PMCID: PMC3634842This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Spectrophotometric evolution of spiral galaxies with truncated star formation: An evolutionary link between spirals and S0s in distant clusters

A one-zone chemo-spectro-photometric model is used to investigate the time evolution of disk galaxies whose star formation is truncated, and to determine the dependence of this evolution on the previous star formation history and the truncation epoch. Truncated spirals show red colors $\sim 1$ Gyr after truncation, and evolve spectrally from an e(b) type, down through the e(a), a+k, and k+a classes, to finally become a k type. The exact behavior in this phase depends on the truncation epoch and the star formation history prior to truncation. For example, earlier type disks show redder colors and do not show a+k-type spectra after truncation of star formation. We also discuss a possible evolutionary link between the k-type galaxies with spiral morphology found in distant clusters, and present-day S0s, by investigating whether truncated spirals reproduce the infrared color-magnitude relation of Coma galaxies. We suggest that only less luminous, later-type disk galaxies whose star formation is truncated at intermediate and high redshifts can reproduce the red $I-K$ colors observed for S0s in the Coma cluster.Comment: 43 pages 19 figures, 2002, ApJ 565, 22

TESS asteroseismology of the known red-giant host stars HD 212771 and HD 203949

International audienc

Analysis of Locally Coupled 3D Manipulation Mappings Based on Mobile Device Motion

We examine a class of techniques for 3D object manipulation on mobile devices, in which the device's physical motion is applied to 3D objects displayed on the device itself. This "local coupling" between input and display creates specific challenges compared to manipulation techniques designed for monitor-based or immersive virtual environments. Our work focuses specifically on the mapping between device motion and object motion. We review existing manipulation techniques and introduce a formal description of the main mappings under a common notation. Based on this notation, we analyze these mappings and their properties in order to answer crucial usability questions. We first investigate how the 3D objects should move on the screen, since the screen also moves with the mobile device during manipulation. We then investigate the effects of a limited range of manipulation and present a number of solutions to overcome this constraint. This work provides a theoretical framework to better understand the properties of locally-coupled 3D manipulation mappings based on mobile device motion