On the capture width of wave energy converters

This paper extends the theory on capture width, a commonly used performance indicator for a wave energy converter (WEC). The capture width of a linear WEC is shown to depend on two properties: the spectral power fraction (a property introduced in this paper), which depends entirely on the sea state, and the monochromatic capture width, which is determined by the geometry of the WEC and the chosen power take off (PTO) coefficients. Each of these properties is examined in detail. Capture width is shown to be a measure of how well these two properties coincide. A study of the effects of PTO control on the capture width suggests that geometry control, a form of control that has not been the focus of much academic research, despite its use in the wave energy industry, deserves more attention. The distinction between geometry control and PTO control is outlined. While capture width is a valuable design tool, its limitations must be recognised. The assumptions made in the formulation of capture width are listed, and its limitations as a tool for estimating annual power capture of a WEC are discussed

Some investigations into non passive listening

Our knowledge of the function of the auditory nervous system is based upon a wealth of data obtained, for the most part, in anaesthetised animals. More recently, it has been generally acknowledged that factors such as attention profoundly modulate the activity of sensory systems and this can take place at many levels of processing. Imaging studies, in particular, have revealed the greater activation of auditory areas and areas outside of sensory processing areas when attending to a stimulus. We present here a brief review of the consequences of such non-passive listening and go on to describe some of the experiments we are conducting to investigate them. In imaging studies, using fMRI, we can demonstrate the activation of attention networks that are non-specific to the sensory modality as well as greater and different activation of the areas of the supra-temporal plane that includes primary and secondary auditory areas. The profuse descending connections of the auditory system seem likely to be part of the mechanisms subserving attention to sound. These are generally thought to be largely inactivated by anaesthesia. However, we have been able to demonstrate that even in an anaesthetised preparation, removing the descending control from the cortex leads to quite profound changes in the temporal patterns of activation by sounds in thalamus and inferior colliculus. Some of these effects seem to be specific to the ear of stimulation and affect interaural processing. To bridge these observations we are developing an awake behaving preparation involving freely moving animals in which it will be possible to investigate the effects of consciousness (by contrasting awake and anaesthetized), passive and active listening

Evidence that stimulation of gluconeogenesis by fatty acid is mediated through thermodynamic mechanisms

AbstractWe have studied the stimulatory effects of palmitate on the rate of glucose synthesis from lactate in isolated hepatocytes. Control of the metabolic flow was achieved by modulating the activity of enolase using graded concentrations of fluoride. Unexpectedly, palmitate stimulated gluconeogenesis even when enolase was rate-limiting. This stimulation was also observed when the activities of phosphoenolpyruvate carboxykinase and aspartate aminotransferase were modulated using graded concentrations of quinolinate and aminooxyacetate, respectively. Linear force-flow relationships were found between the rate of gluconeogenesis and indicators of cellular energy status (i.e. mitochondrial membrane and redox potentials and cellular phosphorylation potential). These findings suggest that the fatty acid stimulation of glucose synthesis is in part mediated through thermodynamic mechanisms

Administration of human chorionic gonadotropin at embryo transfer induced ovulation of a first-wave dominant follicle and increased progesterone and transfer pregnancy rates

Beef Cattle Research, 2011 is known as Cattlemen’s Day, 2011Embryo transfer (ET) has become more widespread in recent years as a way to improve cattle genetics. According to the annual statistical survey of the American Embryo Transfer Association, more than 200,000 fresh and frozen bovine embryos were transferred in 2008. But despite advancements in reproductive technologies that have occurred since ET was commercialized in the 1970s, industrywide pregnancy rates are only 62.4 and 56.9% for fresh and frozen-thawed ET, respectively. Using ET helps avoid problems from failed fertilization; however, fertilization failure has been characterized as a relatively unimportant factor of pregnancy loss. Approximately 10% of pregnancy failures resulted from fertilization failure and another 10% from failed embryo development. Approximately 20 to 25% of the pregnancy loss in an ET program could be characterized as early embryonic loss

Modelling eggshell maculation

The eggshells of many avian species are characterised by distinctive patterns of maculation, consisting of speckles, spots, blotches or streaks, the spatial-statistical properties of which vary considerably between (and often within) species. Understanding the mechanisms underlying the production of eggshell maculation would enable us to explore the costs and constraints on the evolution of maculation patterns, but as yet this area is surprisingly understudied. Here I present a simple model of eggshell maculation, which is based on the known biology of pigment deposition, and which can produce a range of realistic maculation patterns. In particular, it provides an explanation for previous observations of maculation heterogeneity and diversity, and allows testable predictions to be made regarding maculation patterns, including a possible signalling role

Chaperone-mediated native folding of a β-scorpion toxin in the periplasm of E.coli

Background: Animal neurotoxin peptides are valuable probes for investigating ion channel structure/function relationships and represent lead compounds for novel therapeutics and insecticides. However, misfolding and aggregation are common outcomes when toxins containing multiple disulfides are expressed in bacteria. Methods: The ß-scorpion peptide toxin Bj-xtrIT from Hottentotta judaica and four chaperone enzymes (DsbA, DsbC, SurA and FkpA) were co-secreted into the oxidizing environment of the E.coli periplasm. Expressed Bj-xtrIT was purified and analyzed by HPLC and FPLC chromatography. Its thermostability was assessed using synchrotron radiation circular dichroism spectroscopy and its crystal structure was determined. Results: Western blot analysis showed that robust expression was only achieved when cells co-expressed the chaperones. The purified samples were homogenous and monodisperse and the protein was thermostable. The crystal structure of the recombinant toxin confirmed that it adopts the native disulfide connectivity and fold. Conclusions: The chaperones enabled correct folding of the four-disulfide-bridged Bj-xtrIT toxin. There was no apparent sub-population of misfolded Bj-xtrIT, which attests to the effectiveness of this expression method. General Significance: We report the first example of a disulfide-linked scorpion toxin natively folded during bacterial expression. This method eliminates downstream processing steps such as oxidative refolding or cleavage of a fusion-carrier and therefore enables efficient production of insecticidal Bj-xtrIT. Periplasmic chaperone activity may produce native folding of other extensively disulfide-reticulated proteins including animal neurotoxins. This work is therefore relevant to venomics and studies of a wide range of channels and receptors

Recycling sediments between source and sink during a eustatic cycle: Systems of late Quaternary northwestern Gulf of Mexico Basin

The northwestern Gulf of Mexico Basin is an ideal natural laboratory to study and understand source-to-sink systems. An extensive grid of high-resolution seismic data, hundreds of sediment cores and borings and a robust chronostratigraphic framework were used to examine the evolution of late Quaternary depositional systems of the northwestern Gulf of Mexico throughout the last eustatic cycle (~125 ka to Present). The study area includes fluvial systems with a wide range of drainage basin sizes, climate settings and water and sediment discharges. Detailed paleogeographic reconstructions are used to derive volumetric estimates of sediment fluxes (Volume Accumulation Rates). The results show that the response of rivers to sea-level rise and fall varied across the region. Larger rivers, including the former Mississippi, Western Louisiana (presumably the ancestral Red River), Brazos, Colorado and Rio Grande rivers, constructed deltas that advanced across the shelf in step-wise fashion during Marine Isotope Stages (MIS) 5-2. Sediment delivery to these deltas increased during the overall sea-level fall due to increases in drainage basin area and erosion of sediment on the inner shelf, where subsidence is minimal, and transport of that sediment to the more rapidly subsiding outer shelf. The sediment supply from the Brazos River to its delta increased at least 3-fold and the supply of the Colorado River increased at least 6-fold by the late stages of sea-level fall through the lowstand. Repeated filling and purging of fluvial valleys from ~119-22 ka contributed to the episodic growth of falling-stage deltas.During the MIS 2 lowstand (~22-17 ka), the Mississippi River abandoned its falling-stage fluvial-deltaic complex on the western Louisiana shelf and drained to the Mississippi Canyon. Likewise, the Western Louisiana delta was abandoned, presumably due to merger of the Red River with the Mississippi River, terminating growth of the Western Louisiana delta. The Brazos River abandoned its MIS 3 shelf margin delta to merge with the Trinity, Sabine and Calcasieu rivers and together these rivers nourished a lowstand delta and slope fan complex. The Colorado and Rio Grande rivers behaved more as point sources of sediment to thick lowstand delta-fan complexes.Lowstand incised valleys exhibit variable morphologies that mainly reflect differences in onshore and offshore relief and the time intervals these valleys were occupied. They are deeper and wider than falling stage channel belts and are associated with a shelf-wide surface of erosion (sequence boundary).During the early MIS 1 (~17 ka to 7~10 ka) sea-level rise, the offshore incised valleys of the Calcasieu, Sabine, Trinity, Brazos, Colorado, and Rio Grande rivers were filled with sediment. The offshore valleys of smaller rivers of central Texas would not be filled until the late Holocene, mainly by highstand mud. The lower, onshore portions of east Texas incised valleys were filled with sediment mainly during the Holocene, with rates of aggradation in the larger Brazos and Colorado valleys being in step with sea-level rise. Smaller rivers filled their valleys with back-stepping fluvial, estuarine and tidal delta deposits that were offset by flooding surfaces. In general, the sediment trapping capacity of bays increased as evolving barrier islands and peninsulas slowly restricted tidal exchange with the Gulf and valley filling led to more shallow, wider bays. A widespread period of increased riverine sediment flux and delta growth is attributed to climate change during MIS 1, between ~11.5 and 8.0 ka, and occurred mainly under cool-wet climate conditions.Relatively small sea-level oscillations during the MIS 1 transgression (~17 ka to ~4.0 ka) profoundly influenced coastal evolution, as manifested by landward stepping shorelines, on the order of tens of kilometers within a few thousand years. The current barriers, strand plains and chenier plains of the study area formed at different times over the past ~8 ka, due mainly to differences in sand supply and the highly variable relief on the MIS 2 surface on which these systems formed.Modern highstand deposition on the continental shelf formed the Texas Mud Blanket, which occurs on the central Texas shelf and records a remarkable increase in fine-grained sediment supply. This increase is attributed to greater delivery of sediments from the Colorado and Brazos rivers, which had filled their lower valleys and abandoned their transgressive deltas by late Holocene time, and to an increase in westward directed winds and surface currents that delivered suspended sediments from the Mississippi River to the Texas shelf.Collectively, our results demonstrate that source-to-sink analyses in low gradient basin settings requires a long-term perspective, ideally a complete eustatic cycle, because most of the sediment that was delivered to the basin by rivers underwent more than one cycle of erosion, transport and sedimentation that was regulated by sea-level rise and fall. Climate was a secondary control. The export of sediments from the hinterland to the continental shelf was not directly in step with temperature change, but rather varied between different fluvial-deltaic systems

New directions in island biogeography

Aim: Much of our current understanding of ecological and evolutionary processes comes from island research. With the increasing availability of data on distributions and phylogenetic relationships and new analytical approaches to understanding the processes that shape species distributions and interactions, a re-evaluation of this ever-interesting topic is timely. Location: Islands globally. Methods: We start by arguing that the reasons why island research has achieved so much in the past also apply to the future. We then critically assess the current state of island biogeography, focusing on recent changes in emphasis, including research featured in this special issue of Global Ecology and Biogeography. Finally, we suggest promising themes for the future. We cover both ecological and evolutionary topics, although the greater emphasis on island ecology reflects our own backgrounds and interests. Results: Much ecological theory has been directly or indirectly influenced by research on island biotas. Currently, island biogeography is renascent, with research focusing on, among other things, patterns and processes underlying species interaction networks, species coexistence and the assembly of island communities through ecological and evolutionary time. Continuing island research should provide additional insight into biological invasions and other impacts of human activities, functional diversity and ecosystem functioning, extinction and diversification, species pools and more. Deeper understanding of the similarities and differences between island and mainland systems will aid transferability of island theory to continental regions. Main conclusions: As research in biogeography and related fields expands in new directions, islands continue to provide opportunities for developing insights, both as natural laboratories for ecology and evolution and because of the exceptions islands often present to the usual ‘rules’ of ecology. New data collection initiatives are needed on islands world-wide and should be directed towards filling gaps in our knowledge of within-island distributions of species, as well as the functional traits and phylogenetic relationships of island species

Scattering of charge carriers in graphene induced by topological defects

We study the scattering of graphene quasiparticles by topological defects, represented by holes, pentagons and heptagons. For holes, we found that at low concentration they give a negligible contribution to the resistivity. Whenever pentagons or heptagons are introduced we realize that a fermionic current is scattered by defects

Professionalism, Golf Coaching and a Master of Science Degree: A commentary

As a point of reference I congratulate Simon Jenkins on tackling the issue of professionalism in coaching. As he points out coaching is not a profession, but this does not mean that coaching would not benefit from going through a professionalization process. As things stand I find that the stimulus article unpacks some critically important issues of professionalism, broadly within the context of golf coaching. However, I am not sure enough is made of understanding what professional (golf) coaching actually is nor how the development of a professional golf coach can be facilitated by a Master of Science Degree (M.Sc.). I will focus my commentary on these two issues