Characterization of the transcript for a depressant insect selective neurotoxin gene with an isolated cDNA clone from the scorpion Buthotus judaicus

AbstractThe poly(A)+ mRNA isolated from the venomous terminal segments of the scorpion Buthotus judaicus was reversed transcribed into cDNA. PCR amplification of the cDNA in presence of oligonucleotide primers prepared on basis of the known amino acid sequence of the depressant insect toxin II yielded a 125 bp long product. This fragment was cloned and its sequence determined. The deduced amino acid sequence has revealed a complete homology with the amino acid sequence of the toxin. This clone was used to probe a Northern blot resolving the poly(A)+ and poly(A)− fractions derived from the scorpion. An organ specific 360 nucleotide transcript which might be the processed product of a 4̃.0 kb precursor was elucidated. This cDNA clone may pave the way for a molecular genetic approach to study the structure-function relationship of scorpion selective insect toxins

Daylight Saving Time

Currently, in most parts of the United States, timepieces are moved forward one hour in the spring and back one hour in the fall to provide an extended daylight period during the summer months. This is known as Daylight Saving Time (DST). Much debate and many changes led to this present practice. This report provides a brief history of the issues surrounding DST, an outline of the legislation that created and modified it, and a list of references to more discussions

NASA's Voyager Spacecraft: A Fact Sheet

This report discusses the Voyager 2, which was launched on August 20, 1977, from Cape Canaveral. Their current mission is to extend the National Aeronautics and Space Administration (NASA) exploration of the outermost edge of the solar system and the region where the sun’s influence ends

Daylight Saving Time

This report details the information related to daylight saving time. The content includes its history, changing an area's time zone, moving an area on or off DS

Federal Statutes: What They Are and Where to Find Them

This report provides a brief overview of Federal statutes and where to find them, both in hard copy and on the Internet. After providing an overview on the basics of Federal statutes, this report gives guidance on where Federal statutes, in their various forms, may be located on the Internet, where they are most readily accessible

Legislative History: A Basic Guide For Constituents

This report provides an overview of legislative history of federal legislation. The report briefly outlines the legislative process and then suggests where to find materials that describe or document a piece of legislation's progress through Congress

Federal Statutes: What They Are and Where to Find Them

This report provides a brief overview of Federal statutes and where to find them, both in hard copy and on the Internet. After providing an overview on the basics of Federal statutes, this report gives guidance on where Federal statutes, in their various forms, may be located on the Internet, where they are most readily accessibl

Projektiranje i analiza digitalnog sata

Sat je jedan od najstarijih ljudskih otkrića. U principu, potrebno je znati osnovne fizičke procese koji se ponavljaju s određenom učestalošću, i način da se izmjeri koliko taj proces traje. Kao što se godišnja doba i faze mjeseca mogu iskoristiti za mjerenje protoka određenih dužih perioda vremena, tako se i kraći periodi mogu koristiti za mjerenje sati i minuta. Sunčani sat koji mjeri vrijeme dana pomoću smjera sjenke koju baca određeni predmet osvjetljen suncem, bio je dobro poznat u drevnim vremenima. Pješčani satovi mjerili su vrijeme prolaskom sitnog pijeska kroz uzani otvor na staklenoj posudi. Razvoj elektronike u 20. stoljeću doveo je do satova bez ikakvog mehanizma. Vrijeme na ovakvim satovima mjerilo se na razne načine, na primjer pomoću kvarcnih kristala ili raspadanjem radioaktivnih elemenata. Čak su i mehanički satovi napajani baterijama, čime je navijanje sata postalo suvišno. Cilj ovog rada je projektirati i analizirati jedan digitalni zidni sat s integriranim krugom Maxim IC type DS3231 koji je kvalificiran od strane proizvođača kao „iznimno točan I2C sat u stvarnom vremenu (RTC), s integriranim temperaturno kompenziranim kristalnim oscilatorom (TCXO) i kristalom.“[1] Smatra se da integracija kristalnog rezonatora povećava dugotrajnu točnost uređaja, garantirajući maksimalnu pogrešku manju od 64 sekunde u godini, i temperaturni opseg od 0 do 40 °C (32 do 104 °F). Uređaj uključuje baterijski ulaz koji održava rad uređaja u odsutnosti vanjskog izvora.Clock is one of the oldest human inventions. In principle, it is necessary to know basic physical processes which are repeated with a certain frequency and method to measure how much this process lasts. Such as the season and phase of the month can be used to measure flow of certain lengthy periods of time, so are shorter periods used to measure hours and minutes. A sunidial shows the time by displaying the position of shadow on flat surface. Hourglasses measured time by passing of the fine sand through narrow opening on a glass container. The objective of this article is to project and analyze digital wall clock with Maxim IC type DS3231, qualified by its manufacturer as an „extremely accurate I2C real time clock (RTC) with integrated temperature compensated crystal oscillator (TCXO) and crystal.“ It is considered that integration of the crystal resonator enhances the long-term accuracy of derive, guaranteeing a maximum error of less than 64 seconds over a year, and over a temperature range 0 to 40 °C (32 to 104 °F). The device incorporates a battery input which maintains running of the device in the absence of external power