Retencao de fosforo em tecidos de ovinos submetidos a diferentes proporcoes de Ca:P na dieta.

O objetivo deste trabalho foi estudar a retencao de fosforo nos tecidos e fluidos de ovinos submetidos a diferentes proporcoes Ca:P na dieta. Foram utilizados nove ovinos, machos, castrados, mantidos em gaiolas de metabolismo. A racao de mantenca, feno de capim "coast cross", farinha de mandioca e ureia, foi suplementada com Na2HPO4 e CaCO3 para dar as seguintes proporcoes Ca: 0,75:1,0 (T1); 1,5:1,0 (T2) e 3,0:1,0 (T3). Apos 21 dias de adaptacao, cada animal recebeu uma injecao de 7,4 MB1 de 32P na forma de Na2HPO4 e foram coletadas amostras de sangue e fezes poroito dias. Atraves das atividades especificas nas fezes e plasma foi determinada a meia-vida biologica do 32P no plasma. No nono dias apos a injecao de 32P, todos os animais foram sacrificados e coletadas amostras de figado, rim, musculo, coracao e costelas para determinacao das atividades especificas e retencao de 32P nos tecidos. Os teores de P inorganico no plasma e meia-vida biologica do 32P plasma foram de: 4,46; 5,71, 5,62 mg;100 ml e 62,57; 78,82; 70,80 horas, respectivamente para os tratamentos T1, T2 e T3, nao havendo diferenca entre tratamentos. Os valores medios de P inorganico e retencao de 32P nos tecidos foram de 10,86; 9,61; 6,73; 6,54 e 91,09 mg/g de MS e 0,50; 0,31; 0,06; 0,24 e 0,75ùpara figado, rim, musculo, coracao e osso, respectivamente. Conclui-se que para este tipo de racao as diferentes proporcoes Ca:P utilizados nao influiram na retencao de P pelos tecidos

Incorporação de 32P por microrganismos do rúmen usando bagaço de tomate e forrageiras do Semi-Árido como substrato.

A biossíntese de proteína microbiana é um indicativo de qualidade do alimento e pode ser determinada através da incorporação de 32P pelos microrganismos do rúmen. Um ensaio de incubação de líquido ruminal usando como substratos bagaço de tomate, maniçoba, feijão bravo, jurema e capim buffel, foi realizado para determinar a incorporação de 32P a partir destes alimentos. As forragens apresentaram índices de incorporação altos e significativamente superiores (P<0,05) aos do bagaço de tomate

Rumen phosphorus metabolism in sheep

8 páginas, 3 tablas.The objective of the present study was to examine the effect of the level of phosphorus (P) intake on ruminal P kinetics in sheep. Twelve Santa Ines male sheep (average body weight 36 kg) were fed a basal diet consisting of roughage (coast cross hay), concentrate mixture (cassava meal, soya bean meal and urea) and a mineral premix. The treatments consisted of the basal diet supplemented with 0, 1.5, 3 or 4.5 g/kg dry matter (DM) of mono-ammonium phosphate to provide increasing P levels representing treatments T0, T1, T2 and T3, respectively. The P content of experimental diets was 1-5; 2.0; 2.5 and 3.0 g P/kg DM, and considered highly deficient, deficient, adequate and in excess, respectively, compared with standard recommended allowances. Animals were injected with (32)P and thereafter samples of blood were collected over 7 days, while samples of rumen fluid and saliva were collected 4 and 6 days after injection. Phosphorus intake affected P concentration in ruminal fluid, whereas P concentration in saliva was not affected. The values for P turnover time in the rumen were 1.42, 1.23, 1.18 and 1.04 days, whereas values of endogenous P entry into the rumen were 1.05, 1.37, 1.53 and 1.91 g/day for T0, T 1, T2 and T3, respectively, both affected by P intake. The specific activity (SA) of P in saliva, rumen and plasma were also all affected by P intake. The relationship between saliva and rumen SA emphasizes that most endogenous P in the rumen came from saliva. The possibility of an extra P source besides saliva contributing to endogenous P in the rumen is discussed. It is concluded from the results that, regardless of P intake, the flow of endogenous P into the rumen contributes to ensure a minimum Supply of this essential element, which may be important in matching the requirements of the rumen microbes.Fundaçao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP Proc. 2004/14532-5) for financial support. Funding was provided, in part, by the Canada Research Chairs Program.Peer reviewe

Degradação in situ do bagaço de tomate e de algumas forragens do Semi-Árido brasileiro.

A técnica de degradação forrageiras do ação em sacos de náilon tem sido largamente usada na avaliação de alimentos para ruminantes. Três carneiros fistulados no rúmen foram utilizados para avaliar a degradabilidade do bagaço de tomate e de algumas semi-árido brasileiro (capim buffel, maniçoba, feijão bravo e jurema preta). O capim buffel e a maniçoba apresentaram degradabilidades da matéria seca e da proteína bruta crescentes, indicando a necessidade de um tempo maior de incubação. A maniçoba, uma planta nativa da caatinga, apresentou elevados índices de degradabilidade da matéria seca e da proteína bruta

Caracterização de forrageiras do nordeste utilizando a técnica de produção de gases, quantificação de taninos e composição química. 2. Plantas do estado do Ceará.

Objetivou-se caracterizar por meio da técnica ?in vitro?, quantificação de taninos e análises químicas o potencial forrageiro de quatro espécies de plantas: catingueira 'Caesalpinia bracteosa', jucazeiro 'Caesalpinia ferrea', jurema preta 'Mimosa hostilis' e sabiá 'Mimosa caesalpinifolia'. Os conteúdos de fenóis totais e taninos totais foram analisados pelo método Folin-Ciocalteu e taninos condensados pelo método butanol-HCl e a avaliação da qualidade das plantas foi feita pela técnica de produção de gases. Jucazeiro e catingueira foram as plantas que apresentaram menores valores de taninos condensados e os melhores resultados em relação à produção potencial de gases. As plantas com altos teores de taninos condensados podem interferir no consumo dos animais

Impact of Baseline Retinal Nonperfusion and Macular Retinal Capillary Nonperfusion on Outcomes in the COPERNICUS and GALILEO Studies

To evaluate the impact of baseline retinal capillary nonperfusion (RNP) and macular retinal capillary nonperfusion (MNP) status on outcomes at week&nbsp;24 (W24)

Observation of an Excited Bc+ State

Using pp collision data corresponding to an integrated luminosity of 8.5 fb-1 recorded by the LHCb experiment at center-of-mass energies of s=7, 8, and 13 TeV, the observation of an excited Bc+ state in the Bc+π+π- invariant-mass spectrum is reported. The observed peak has a mass of 6841.2±0.6(stat)±0.1(syst)±0.8(Bc+) MeV/c2, where the last uncertainty is due to the limited knowledge of the Bc+ mass. It is consistent with expectations of the Bc∗(2S31)+ state reconstructed without the low-energy photon from the Bc∗(1S31)+→Bc+γ decay following Bc∗(2S31)+→Bc∗(1S31)+π+π-. A second state is seen with a global (local) statistical significance of 2.2σ (3.2σ) and a mass of 6872.1±1.3(stat)±0.1(syst)±0.8(Bc+) MeV/c2, and is consistent with the Bc(2S10)+ state. These mass measurements are the most precise to date

Bose-Einstein correlations of same-sign charged pions in the forward region in pp collisions at √s=7 TeV

Bose-Einstein correlations of same-sign charged pions, produced in protonproton collisions at a 7 TeV centre-of-mass energy, are studied using a data sample collected by the LHCb experiment. The signature for Bose-Einstein correlations is observed in the form of an enhancement of pairs of like-sign charged pions with small four-momentum difference squared. The charged-particle multiplicity dependence of the Bose-Einstein correlation parameters describing the correlation strength and the size of the emitting source is investigated, determining both the correlation radius and the chaoticity parameter. The measured correlation radius is found to increase as a function of increasing charged-particle multiplicity, while the chaoticity parameter is seen to decreas

Study of J /ψ production in Jets

The production of J/ψ mesons in jets is studied in the forward region of proton-proton collisions using data collected with the LHCb detector at a center-of-mass energy of 13 TeV. The fraction of the jet transverse momentum carried by the J/ψ meson, z(J/ψ)≡pT(J/ψ)/pT(jet), is measured using jets with pT(jet)>20 GeV in the pseudorapidity range 2.5<η(jet)<4.0. The observed z(J/ψ)distribution for J/ψ mesons produced in b-hadron decays is consistent with expectations. However, the results for prompt J/ψ production do not agree with predictions based on fixed-order nonrelativistic QCD. This is the first measurement of the pT fraction carried by prompt J/ψ mesons in jets at any experiment

Study of charmonium production in b -hadron decays and first evidence for the decay Bs0

Using decays to φ-meson pairs, the inclusive production of charmonium states in b-hadron decays is studied with pp collision data corresponding to an integrated luminosity of 3.0 fb−1, collected by the LHCb experiment at centre-of-mass energies of 7 and 8 TeV. Denoting byBC ≡ B(b → C X) × B(C → φφ) the inclusive branching fraction of a b hadron to a charmonium state C that decays into a pair of φ mesons, ratios RC1C2 ≡ BC1 /BC2 are determined as Rχc0ηc(1S) = 0.147 ± 0.023 ± 0.011, Rχc1ηc(1S) =0.073 ± 0.016 ± 0.006, Rχc2ηc(1S) = 0.081 ± 0.013 ± 0.005,Rχc1 χc0 = 0.50 ± 0.11 ± 0.01, Rχc2 χc0 = 0.56 ± 0.10 ± 0.01and Rηc(2S)ηc(1S) = 0.040 ± 0.011 ± 0.004. Here and below the first uncertainties are statistical and the second systematic.Upper limits at 90% confidence level for the inclusive production of X(3872), X(3915) and χc2(2P) states are obtained as RX(3872)χc1 < 0.34, RX(3915)χc0 < 0.12 andRχc2(2P)χc2 < 0.16. Differential cross-sections as a function of transverse momentum are measured for the ηc(1S) andχc states. The branching fraction of the decay B0s → φφφ is measured for the first time, B(B0s → φφφ) = (2.15±0.54±0.28±0.21B)×10−6. Here the third uncertainty is due to the branching fraction of the decay B0s → φφ, which is used for normalization. No evidence for intermediate resonances is seen. A preferentially transverse φ polarization is observed.The measurements allow the determination of the ratio of the branching fractions for the ηc(1S) decays to φφ and p p asB(ηc(1S)→ φφ)/B(ηc(1S)→ p p) = 1.79 ± 0.14 ± 0.32