Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide

A simple and economical minibioreactor-gas collector system for determination of hydrogen sulfide produced by a microorganism was designed. The detection of hydrogen sulfide was based on the reaction between the hydrogen sulfide in the gas stream from the culture, and a lead acetate solution 0.090 mol/L, contained in a tube gas collector; the conductimetric back titration of lead excess was made, and hydrogen sulfide was quantified indirectly, being the detection limit 0.5 \u3bcmol. The developed system was applied using Tsukamurella paurometabola DSM 20162 as a model, being the amount of hydrogen sulfide produced, 1.2 \u3bcmol in 24 hrs

Excitation of vibrational modes in the ionization of water molecule by XUV/X-ray radiation

We present a theoretical study of the vibrationally resolved core photoionization of the water molecule up to high photon energies. In order to understand the role of the coupled electron-nuclear motion in polyatomic molecules, we thus have implemented a new methodology to describe all vibrational modes of a polyatomic molecule. We show our preliminary results on the O(1s) photoionization, with special focus on the vibrationally resolved cross sections in a large range of photon energies, reaching up to 1500 e

The cristae modulator Optic atrophy 1 requires mitochondrial ATP synthase oligomers to safeguard mitochondrial function

It is unclear how the mitochondrial fusion protein Optic atrophy 1 (OPA1), which inhibits cristae remodeling, protects from mitochondrial dysfunction. Here we identify the mitochondrial F1Fo-ATP synthase as the effector of OPA1 in mitochondrial protection. In OPA1 overexpressing cells, the loss of proton electrochemical gradient caused by respiratory chain complex III inhibition is blunted and this protection is abolished by the ATP synthase inhibitor oligomycin. Mechanistically, OPA1 and ATP synthase can interact, but recombinant OPA1 fails to promote oligomerization of purified ATP synthase reconstituted in liposomes, suggesting that OPA1 favors ATP synthase oligomerization and reversal activity by modulating cristae shape. When ATP synthase oligomers are genetically destabilized by silencing the key dimerization subunit e, OPA1 is no longer able to preserve mitochondrial function and cell viability upon complex III inhibition. Thus, OPA1 protects mitochondria from respiratory chain inhibition by stabilizing cristae shape and favoring ATP synthase oligomerization

Geographical variation in therapy for bloodstream infections due to multidrug-resistant enterobacteriaceae: a post hoc analysis of the INCREMENT study

We aimed to describe regional differences in therapy for bloodstream infection (BSI) caused by extended-spectrum ?-lactamase-producing Enterobacteriaceae (ESBL-E) or carbapenemase-producing Enterobacteriaceae (CPE). 1,482 patients in 12 countries were included from an observational study of BSI caused by ESBL-E or CPE. Multivariate logistic regression was used to calculate adjusted odds ratios (aORs) for the influence of country of recruitment on empirical use of ?-lactam/?-lactamase inhibitors (BLBLI) or carbapenems, targeted use of BLBLI for ESBL-E and use of targeted combination therapy for CPE. The use of BLBLI for empirical therapy was least likely in sites from Israel (aOR 0.34, 95% CI 0.14-0.81), Greece (aOR 0.49, 95% CI 0.26-0.94) and Canada (aOR 0.31, 95% CI 0.11-0.88) but more likely in Italy (aOR 1.58, 95% CI 1.11-2.2) and Turkey (aOR 2.09, 95% CI 1.14-3.81), compared to Spain as a reference. Empirical carbapenems were more likely to be used in sites from Taiwan (aOR 1.73, 95% CI 1.03-2.92) and USA (aOR 1.89; 95% CI 1.05-3.39), and less likely in Italy (aOR 0.44, 95% CI 0.28-0.69) and Canada (aOR 0.10, 95% CI 0.01-0.74). Targeted BLBLI for ESBL-E was more likely in sites from Italy. Treatment at sites within Israel, Taiwan, Turkey and Brazil was associated with less combination therapy for CPE. Although this study does not provide precise data on the relative prevalence of ESBL-E or CPE, significant variation in therapy exists across countries even after adjustment for patient factors. A better understanding of what influences therapeutic choices for these infections will aid antimicrobial stewardship efforts.PH is supported by an Australian Postgraduate Award from the University of Queensland. The study was funded by the Ministerio de Economía y Competitividad, Instituto de Salud Carlos III - co-financed by European Development Regional Fund "A way to achieve Europe" ERDF, Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015). BGG, JRB, APH and YC also received funds from the COMBACTE-CARE project (grant agreement 115620), Innovative Medicines Initiative (IMI), the European Union's Seventh Framework Programme (FP7/2007-2013) and in-kind contributions from EFPIA companies

Las TIC para la pr\ue1ctica de la melod\ueda en el aula de m\ufasica :

Trabajo Fin de Grado-Universidad Pontificia de Salamanca, Facultad de Educaci\uf3n, 2019La sociedad en general, ha experimentado un cambio estos \ufaltimos a\uf1os con las TIC (Tecnolog\uedas de la Informaci\uf3n y la Comunicaci\uf3n), lo que facilita a la comunidad educativa su uso para una ense\uf1anza de calidad. Estas tecnolog\uedas traen consigo ventajas e inconvenientes que el profesorado, con su continua formaci\uf3n, deben potenciar o minimizar para conseguir los objetivos propuestos en ense\uf1anza. Este Trabajo de Fin de Grado (TFG) proporciona informaci\uf3n sobre el uso de las Tecnolog\uedas de la Informaci\uf3n y la Comunicaci\uf3n en las aulas de primaria. La necesidad de crear melod\uedas, por medio de aplicaciones, facilita el desarrollo del alumno, fomentando la creatividad y proporcionando nuevas formas de explorar el mundo que nos rodea para que el alumno sea capaz de construir su propio pensamiento. La propuesta did\ue1ctica facilitar\ue1 la comprensi\uf3n y el trabajo a realizar en el aula de m\ufasica

Scutellaria cuevasiana and Scutellaria sublitoralis (Lamiaceae), two new species from Jalisco and Nayarit, Mexico

AbstractScutellaria cuevasiana J. G. González et A. Vázquez and Scutellaria sublitoralis J. G. González are described and illustrated. Scutellaria cuevasiana fits best within section Uliginosae(Epling) Epling. It is morphologically close to S. blepharophylla Epling, S. rosei Fernald and S. seleriana Loes. It differs from them by its magenta corollas, small and sessile floral bracts, longer petioles and bigger leaves. Scutellaria sublitoralis does not manifest any clear morphological affinity with species of the sections enumerated by Epling. It could be related to species in sections Pallidiflorae Epling or Uliginosae, particularly with the “Scutellaria ovata” or “ Scutellaria caerulea” species groups defined by Paton. It is morphologically similar to S. pallidiflora Epling; however, it can be recognized by wider leaves with usually cordate to subcordate bases, persistent floral bracts, floral axis and calyces without glandular-capitate hairs, longer corollas, and narrower toward the throat

Salvia carreyesii, Salvia ibugana and Salvia ramirezii (Lamiaceae), three new species from Jalisco, Mexico

AbstractThree new species from Jalisco, Mexico, are described and illustrated. These species grow in tropical or in tropical and temperate transitional forests. The 3 species belong to Salvia L. subgenus Calosphace (Benth.) Benth. Salvia carreyesii J. G. González is morphologically similar with section Briquetia Epling. It is characterized by the contrasting vegetative morphology between mature and immature individuals, its relatively large and sessile or sub-sessile leaves (the uppermost amplexicaul), glandular-capitate hairs on the floral axis and calyx, and dark violet corollas. The characters of Salvia ibugana J. G. González correspond to those of the species of section Angulatae (Epling) Epling, within which S. ibugana is distinguished by the digitiform papillae disperse on its stems, petioles and floral axis. Salvia ramirezii J. G. González fits well within section Sigmoideae Epling by means of the sigmoid shape of the lower branch of its style. It is morphologically similar to Salvia crucis and S. quercetorum, from which it can be distinguished by the triangular, slightly succulent, shorter, glabrous, lustrous leaves

Food habits of the yellow snapper Lutjanus argentiventris (Peters, 1869) (Percoidei: Lutjanidae) in La Paz Bay, Mexico

A total of 304 yellow snapper Lutjanus argentiventris stomachs were sampled bimonthly from April 2003 to April 2004, of which 44% contained food. We identified 54 prey species. From the index of relative importance (IRI), fish eggs (62%), Harengula thrissina (23%), Porichthys margaritatus (3.3%), and Abudefduf troschelii (2.3%) were the prey of greatest importance in its trophic spectrum. In juvenile snappers, the most important preys were unidentified organic matter (32%), the crustacean Upogebia pugettensis (29%), penaeid shrimp (6.5%), and fish eggs (5.2%). The Levin index determined that the diet breadth was low (Bi=0.0002), indicating that it is a specialist predator showing preference for fish eggs and H. thrissina. The same result was found in males (Bi=0.0002) and females (Bi = 0.0009). Although juveniles also showed a low trophic breadth, they preferred unidentified organic matter and U. pugettensis. The Morisita-Horn index showed considerable overlap in diet between genders (Cλ>0.6), though diet overlap was low between juveniles and adults (Cλ0,6), mientras que para juveniles y adultos se registró un traslapo bajo (Cλ<0,6), reflejando la diferencia de su hábitat